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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... MCF-7 and T47D were purchased from ATCC and were cultured in phenol red–free DMEM (Gibco) with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... using a QuantStudio 7 Flex Real-Time PCR System utilizing TaqMan Gene Expression Master Mix (Applied Biosystems).
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bioRxiv - Biochemistry 2023Quote: ... and 10% glycerol) using 0.5-mL Zeba™ spin desalting column (7-kDa MWCO) (ThermoFisher Scientific, #89882). The solution was warmed to room temperature and 5 μL of 5 mg/mL taxol-stabilized MTs was added ...
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bioRxiv - Immunology 2024Quote: ... and qPCR experiments were completed on a QuantStudio 7 flex real-time PCR system (Applied Biosystems, 4485701) using the Taqman gene expression master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: The brains of 7-day-old flies were dissected in Schneider’s insect medium (Thermo Fisher Scientific, 21720). They were then fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and transfected with indicated siRNAs (listed in Supplementary table 7) with the use of Lipofectamine RNAiMAX (Invitrogen), according to manufacturer’s instructions using Lipofectamine RNAiMAX (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were dissected from pregnant mice at embryonic day 7.7–7.8 (wild type) or 8.0 (iv/iv) in Dulbecco’s Modified Eagle Medium (DMEM, 12320-032, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2023Quote: ... Single mES cells (7 × 105 cells) were transfected with vectors using Lipofectamine LTX (#15338100; Thermo Fisher Scientific) [DNA ...
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bioRxiv - Genomics 2023Quote: ... MCF-7 breast cancer cells were seeded for 24 hours in phenol red-free MEM medium (GIBCO) supplemented with 5% charcoal-stripped calf serum (Atlanta Biologicals) ...
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bioRxiv - Immunology 2023Quote: ... Relative quantification of genes of interest was performed by qPCR analysis using QuantStudio Pro 7 system (ThermoFisher) and PowerTrack SYBR Green master mix (ThermoFisher) ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... Non-Tg and PS19 neurons were transfected with various constructs at DIV5-7 using Lipofectamine 2000 (Invitrogen) followed by time-lapse imaging 10-14 days after transfection prior to quantification analysis.
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bioRxiv - Bioengineering 2024Quote: ... Herceptin was buffer exchanged in PBS by using 7 K MWCO Zeba Spin Desalting Columns (ThermoFisher Scientific). Then it was incubated with 20ME (Molar equivalent ...
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bioRxiv - Bioengineering 2024Quote: ... and passaged every 7 days by treatment with TrypLE Select Enzyme (Thermo Fisher Scientific Inc., MA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were run and data analyzed using the QuantStudioTM 7 Flex Real-time PCR system (Applied Biosystems). Data were calculated using the 2-△△Ct method.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher) at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR products were detected using an Applied Biosystems ViiA 7 Real-Time PCR System (ThermoFisher Scientific). Relative gene expression was determined by the 2-ΔΔCt method ...
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bioRxiv - Immunology 2024Quote: ... Frozen lungs were cut into 7-8 µm sections using a Cryostar NX50 cryostat (Thermo Fisher Scientific) and mounted on silane-treated glass slides (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... Relative quantification of genes of interest was performed by qPCR analysis using QuantStudio Pro 7 system (ThermoFisher) and SYBR Green master mix (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred to nitrocellulose membranes using the iBlot 7-min blotting system (Thermo Fisher Scientific). Membranes were blocked for 1 h at room temperature in 1 x casein blocking buffer (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... the residual medium was aspirated and 3.5 ml of the collected medium supplemented with 2 mM BrU (ThermoFisher) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid transfections were performed using 2 μg plasmid per 3.5-cm well plates using Lipofectamine 2000 (Life Technologies) transfection reagent according to the manufacturer’s recommendations at 48 hours before harvest ...
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bioRxiv - Microbiology 2020Quote: ... upon which 1 μg of yeast RNA and 10× structure buffer (0.1 M Tris at pH 7, 1 M KCl, 0.1 M MgCl2; Ambion) were added ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... cultured neurons were transfected at 7 days in vitro (DIV) with a commercial calcium phosphate transfection kit (Invitrogen) as previously described49 ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR was performed using let-7 and 2S rRNA TaqMan MicroRNA Assay primer mix (Thermo Fisher Scientific) with the TaqMan Small RNA Assay (Applied Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Cell Biology 2020Quote: Following buffer exchange to 100 mM NaHCO3 with 0.5 ml 7 kDa Spin Desalting Columns (Thermo Fisher, 89882), the anti-TFAM (TA332462 ...
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bioRxiv - Cell Biology 2020Quote: ... 7- Amino-4-Chlormethylcumarin (CMAC) was part of the Yeast Vacuole Marker Sampler Kit (Y7531) from Thermo Fisher and staining was performed according to manufacturer recommendations ...
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bioRxiv - Physiology 2019Quote: ... Malpighian tubules from 7-day-old adult female flies were dissected under Schneider’s insect medium (Thermo Fisher Scientific) and isolated into 10 μl drops of a 1:1 mixture of Schneider’s medium ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... roots of 7-day-old seedlings were incubated with 50 µM Amplex™ UltraRed Reagent (AUR, Molecular Probes) for 20 min in dark conditions and rinsed with liquid MS ...
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bioRxiv - Microbiology 2021Quote: ... mRNA expression of the indicated genes was analyzed by QuantStudio 7 Flex Real Time PCR System (Applied Biosystems) using SYBR green master mix ...
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bioRxiv - Microbiology 2020Quote: ... Vero E6 and Huh-7 cells (Apath LLC, Brooklyn) were maintained in Dulbecco’s modified eagle medium (DMEM, Gibco) supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: EV-rich fractions (7-10) from SEC were initially determined by protein (Pierce™ BCA Protein Assay, ThermoFisher) and particle quantification using nanoparticle tracking analysis (NTA ...
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bioRxiv - Bioengineering 2021Quote: ... Apoptosis was detected by incubating devices with 5μM of CellEvent™Caspase 3/7 Green detection reagent (Invitrogen) for 30 minutes at 37°C.
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bioRxiv - Biochemistry 2020Quote: ... Hs00961560_m1 Hs INSR) and the QuantStudio™ 7 Flex Real-Time PCR system (Thermo Fisher Scientific, MA, USA) and results analysed by the comparative Ct method ...
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bioRxiv - Cancer Biology 2020Quote: ... were used to analyse mRNA expression levels using a QuantStudio 7 Flex RT PCR machine (Thermo Fisher Scientific). TaqMan probes used were FAP (Hs00990791_m1) ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a total volume of 10 μl on a ViiA 7 system (Thermo Fisher Scientific). Gapdh was used as a housekeeping gene and data were evaluated by conversion to ΔCt values.
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bioRxiv - Cell Biology 2021Quote: ... Both COS-7 and HEK 293T were transfected by means of Lipofectamine and PLUS reagent (Thermo Fisher Scientific). The calcium phosphate transfection method was instead used to transfect WT or ΔOLF Lphn2-HA-pCCL in HEK 293T cells and to produce the corresponding viral particles subsequently employed to transduce ECs ...
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bioRxiv - Microbiology 2021Quote: ... a 20μL reaction was loaded into a ViiA 7 Real Time PCR system (Thermo Fisher Scientific, Waltham, MA). The following conditions were used ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR analysis was performed using a sequence detection system (ABI ViiA 7 system; Thermal Fisher Scientific). The following primers sets were used for analysis ...
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bioRxiv - Neuroscience 2020Quote: ... 2x SYBR master mix (Thermoscientific) were used and the reaction was run in Thermocycler (Applied Biosystems ViiA 7) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... MEL cell line-derived clones 4D7 and 2M12 (7) were maintained in 20 µg/mL of blasticidin (Invitrogen) and 1 mg/mL of G418 (Gibco) ...
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bioRxiv - Biophysics 2020Quote: Microinjection was performed in COS-7 cell line cultured in 10% (v/v) fetal bovine serum (Life technologies) DMEM at 1·105 cell/well density ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... were used for experiments in passage 4 to 7 and were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of 7-amino-actinomycin D (7AAD, 50 μg/ml working solution in PBS, Thermo Fisher Scientific) was added to the stained cells 5–10 min before measurement.