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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: For the functionality validation of miR-34a mimic (MC11030, Thermo Fisher), PC3 cells were seeded onto individual wells of a 96-well plate ...
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bioRxiv - Plant Biology 2021Quote: ... The coding sequence of OsSWEET11b was PCR amplified without STOP codon (primers: Table S1) using Phusion High-Fidelity PCR polymerase (Thermo Fisher Scientific) and cloned into the Gateway donor vector pDONR221 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Diluted cDNA was used for qRT-PCR using gene specific primers (Table S2) and Power SYBR Green PCR Master Mix (Applied Biosystems, A25742). Gapdh was used as an endogenous control.
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bioRxiv - Cancer Biology 2022Quote: ... This single-stranded cDNA was then used to perform quantitative real-time PCR (qRT-PCR) with specific primers using PowerUP SYBR Green 2x Master Mix (Thermo Fisher Scientific) on a 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... PCR amplification was performed with barcoded reverse PCR primers in 25 cycles in Taq DNA Pol reaction buffer 1 × (Thermo Fisher Scientific), with 1 μM of each reverse barcoded primer and forward primer (Table SI1) ...
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bioRxiv - Cell Biology 2023Quote: ... was performed on 12 ng cDNA with SybrGreen PCR mastermix (LifeTechnologies) and specific primers (supplementary Table 2) and run in an ABI? real-time PCR machine (Applied Biosystems, ThermoFisher Scientific). The raw data was analysed through -ΔCt as described in Supplementary Methods.
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was performed with specific primers (Supplemental Table S4) using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) and cycling conditions for PCR were 50°C for 2 min ...
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bioRxiv - Biochemistry 2021Quote: ... 500 ng RNA of the total isolated RNA were used as a template for the synthesis of cDNA using Oligo(dT) primers and the Superscript™ II RT protocol (Invitrogen). qPCR was performed using ORA qPCR Green ROX L Mix (HighQu ...
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bioRxiv - Molecular Biology 2021Quote: Reverse Transcription (RT) was performed with 2 μg total RNA and using 3.2 μg random hexamer primers (ThermoFisher Scientific, Cat# SO142) and Transcriptor Reverse Transcriptase (Roche ...
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bioRxiv - Genetics 2019Quote: ... This RNA was converted to cDNA using an oligo dT primer (RT-Uni) and Superscript III (ThermoFisher Scientific, Waltham, MA, USA) for use as a PCR template ...
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bioRxiv - Microbiology 2021Quote: ... The product was used as a template for reverse transcription (RT) with a linker-specific reverse primer using TaqMan Reverse Transcription Reagents from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots of the RNA preparations were subjected to RT with primer PI (5’-AGGCTTGCAAACGGAGTCTAA-3’) and RevertAid reverse transcriptase (Thermo Scientific). RT products were amplified by PCR with Thermus thermophilus DNA polymerase (Biotools ...
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bioRxiv - Cell Biology 2021Quote: ... Individual cells were sorted into 96-well plates containing 4uL of lysis buffer with specific barcoded RT primers (final concentration: 0,2% Triton, 0,4 U/μL RNaseOUT (Thermofisher Scientific, Waltham, Massachussetts), 400nM idx_RT_primers) ...
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bioRxiv - Immunology 2021Quote: ... RRV cDNA was generated from 10 μl of serum derived RNA or 1 μg of tissue derived RNA using a sequence-tagged (indicated with lower case letters) RRV-specific RT primer (5′-ggcagtatcgtgaattcgatgcAACACTCCCGTCGACAACAGA-3′) with SuperScript IV reverse tran-scriptase (Life Technologies). RRV genomes were quantified by RT-qPCR using a tag sequence-specific reverse primer (5′-GGCAGTATCGTGAATTCGATGC-3′ ...
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bioRxiv - Molecular Biology 2022Quote: Immunopurifed RNA (45 ng) was reverse transcribed (RT) with U6 and U2 snRNA reverse primers using SuperScript™ III Reverse Transcriptase (ThermoFisher), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1μg of RNA was reverse transcribed to cDNA by using oligo-dT primers and MMLV-RT (Invitrogen, Waltham, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: 10 ng of total RNA was reverse transcribed with MegaplexTM miRNA-specific stem-loop RT Primers Human Pool A v 2.1 (Thermo Fisher Scientific) and TaqMan® MicroRNA reverse transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse Transcription (RT) was performed on 500 ng of RNA with random primers and SuperScript II Reverse Transcriptase (Thermo Fisher Scientific).
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bioRxiv - Genomics 2024Quote: ... Reverse Transcription (RT) was performed with 1 μg total RNA and using 0.2 μg random hexamer primers (ThermoFisher Scientific, Cat# SO142) and 200 U SuperScript® IV Reverse Transcriptase (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... First-strand cDNA was synthesized using a ratio of 2:1 random primers:oligo (dT) primer and SuperScript II RT (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR reaction was performed using the ViiA7 Real-Time PCR System (Thermo Fisher, USA) in 384-well plates ...
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bioRxiv - Plant Biology 2019Quote: Viral progeny was analyzed by reverse transcription (RT)-PCR using RevertAid reverse transcriptase (Thermo Scientific) and Phusion DNA polymerase followed by electrophoretic separation of the amplification products in 1% agarose gels that were stained with ethidium bromide ...
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bioRxiv - Cell Biology 2020Quote: Real-time RT-PCR was performed in 96-well optic plates (Applied Biosystems, N801-0560) in an Applied Biosystems ABI7500 thermocycler ...
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bioRxiv - Cancer Biology 2019Quote: ... RT-qPCR was performed on an ABI 7900HT Fast Real Time PCR machine (Applied Biosystems) and analysed on SDS Biosystem software ...
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bioRxiv - Immunology 2021Quote: ... cDNA was obtained using Verso cDNA kit Reverse Transcription for RT-PCR (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT–qPCR was performed with Real-Time PCR System (Applied Biosystems, Quantstudio 7 Flex), using SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... Thermal cycling was run on a Step-One Plus RT-PCR thermal cycler (Applied Biosystems) with the following cycle parameters ...
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bioRxiv - Immunology 2019Quote: ... Quantitative RT-PCR was performed using the Fast SYBR™ Green Master Mix (Applied Biosystems). Samples were processed using the BioRad CFX96 Real-Time System ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR analysis was performed using the Power SYBR Green PCR master mix (Thermo Fisher) on the QuantStudio 6 Flex RT-PCR system ...
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bioRxiv - Microbiology 2019Quote: ... and the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen). For all experiments ...
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bioRxiv - Genetics 2019Quote: RT-PCR was conducted using SuperScript IV first-Strand synthesis system (ThermoFisher Scientific, Waltham, MA). Exon 14b was amplified with primers F ...
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bioRxiv - Cancer Biology 2021Quote: ... All RT-qPCRs were run on a QuantStudioTM 3 Real-Time PCR Machine (Applied Biosystems).
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bioRxiv - Cancer Biology 2020Quote: ... RT–PCR was performed using the Veriti ™ 96-Well Fast Thermal Cycler (Applied Biosystems) and real-time qPCR was performed with the StepOnePlus™ real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative RT-PCR using SYBR Green (Quantabio) was performed via QuantStudio 3 system (Applied Biosystems). Primers are listed in Supplemental Table 4.
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bioRxiv - Cancer Biology 2020Quote: ... Complementary DNA (cDNA) was synthesized and amplified using a One Step RT PCR kit (Invitrogen) with PDLIM2-specific primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real time quantitative PCR (RT-qPCR) was performed using SYBR Green Master mix (Applied Biosystems) following manufacturer indications ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR were performed on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher) using 5 μL Power SYBR Green PCR Mix (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... we used the RNA UltraSense™ One-Step Quantitative RT-PCR System from Applied Biosystems™ (11732927 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT-PCR (qPCR) experiments were performed with Fast SYBR Green master mix (Applied Biosystems) according to manufacturer’s instructions and analysed on a QuantStudio Real-Time PCR Light Cycler (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the ABI-PRISM 7900HT Fast Real-Time instruments (Applied Biosystems) by using optical-grade 96-well plates ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were performed on the StepOnePlus™ Real-Time PCR System (Applied Biosystems). Gag primers used were FWD ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative RT-PCR was performed using the Power SYBR® Green Master mix (Applied Biosystems) on 7500 Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... All mRNA RT-qPCRs were performed on a 7500 REAL TIME PCR SYSTEM (Applied Biosystems). mRNA real time quantification was generally performed in a two step format using Eurogentec Reverse Transcriptase Core Kit and MESA GREEN qPCR Master Mix Plus for SYBR Assay with Low Rox kit from Eurogentec following the suppliers’ protocols ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... RT-PCR reactions were performed using the TaqMan Gene Expression Master Mix (Thermo Fisher, 4369016). The following TaqMan Gene Expression Assays primers were obtained from Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated using the SuperScript III one-step RT-PCR (#12574018, ThermoFisher Scientific, USA) and random hexamers (#48190011 ...
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bioRxiv - Cancer Biology 2022Quote: ... RT-qPCR was performed using a StepOnePlus Real-Time PCR system (Applied Biosystems, CA, USA) and PowerUp SYBR Green Master Mix (A25918 ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR reactions were performed on QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) and analyzed using QuantStudio 12K Flex Applied Biosystems software v1.2.3 ...
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time RT-PCR was performed using the SYBR Green Master mix kit (ThermoFisher). Primer sequences were as follows:
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bioRxiv - Developmental Biology 2022Quote: ... RNA was reverse transcribed using Superscript III First-Strand Synthesis System for RT-PCR (Invitrogen), and cDNA was amplified and detected using TaqMan Universal PCR master mix (Applied Biosystems ...