Labshake search
Citations for Thermo Fisher :
1551 - 1600 of 2644 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... was cultured at 2-8×105 cells/ml in Fischer’s medium with 20% horse serum and 2 mM L-glutamine (all Gibco).
-
bioRxiv - Developmental Biology 2021Quote: ... Samples on day 8 or 10 were dissociated with enzyme mix composed of 0.25% Trypsin (Thermo Fisher Scientific, 25200056), and 0.05×DNaseI (STEMCELL Technologies ...
-
bioRxiv - Neuroscience 2022Quote: C57BL/6J mice (8-14 weeks) were injected with CypHer-Aβ (ipsilateral) and NHS-Fluorescein (contralateral, Thermo Fisher 46410) as previously described60 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were settled onto 8-well glass chamber slides (Nunc™ Lab-Tek™ II Chamber Slide™ System) and stained according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Between 8-15 μg of DNA was incubated with 100 μl (~10 mg) of Dynabeads MyOne Streptavidin C1 (Invitrogen) for 15 min with rotation ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
-
bioRxiv - Biochemistry 2019Quote: ... fluorescent probes Prodan (6-propionyl-2[dimethylamino]-naphthalene) and ANS (1-anilinonaphthalene-8-sulfonic acid) were also from Invitrogen; PMB ...
-
bioRxiv - Evolutionary Biology 2020Quote: We mixed 0.8 μl of PCR product with 8.8 μl of formamide and 0.4 μl of marker Liz 500 (Applied Biosystems). We heated the mixture to 95°C for 5 min and let it cool down ...
-
bioRxiv - Developmental Biology 2019Quote: ... chilled on ice for 5 min and electrophoresed in a 3 – 8% Tris-Acetate NuPAGE gel (Novex Life Technologies). Transfer of the proteins onto a nitrocellulose membrane was done in transfer buffer (5% methanol ...
-
bioRxiv - Cell Biology 2019Quote: ... 60 000 cells/ml were seeded in 8-well Nunc™ Lab-Tek™ II Chambered Coverglass (Thermo Fisher) and differentiated for 5-7 days by adding 10 μM all-trans retinoic acid (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: 40 ng of RNA was amended with 8 μl of a 10,000X dilution of External RNA Control Consortium47 (ERCC) Spike-In Mix 1 (Ambion). The ERCC mix consists of 92 transcripts ranging from 250 to 2,000 nt in length with a large dynamic fold range ...
-
bioRxiv - Genomics 2019Quote: ... The cells were then seeded onto Matrigel-coated 6-well plates containing Essential 8™ Medium (ThermoFisher #A15169-01) with added Y-27632 for recovery following nucleofection ...
-
bioRxiv - Biochemistry 2020Quote: Oligonucleotides were supplied PAGE-purified by IDT and resuspended to 100 μM in TE buffer pH 8 (Invitrogen, #AM9849). Equimolar amounts were mixed in 200 μL aliquots at 40μM in TE supplemented with 50 mM NaCl ...
-
bioRxiv - Cancer Biology 2019Quote: ... 5-8×104 cells in 100 μL of 0.9x PBS were loaded into EZ Single Cytofunnel (ThermoFisher Scientific A78710003) containing coated Shandon Cytoslides (ThermoFisher Scientific 5991056 ...
-
bioRxiv - Genomics 2021Quote: ... we seeded cells onto Geltrex-coated Nunc Lab-Tek 8-well chambered coverglass (Thermo Scientific, cat. 12-565-470) at a density of 4-5×104 cells per well ...
-
bioRxiv - Immunology 2021Quote: ... and proteins with a molecular weight above 100 kDa were separated on 3-8% Tris/Acetate gels (Life Technologies) in MOPS Buffer (Invitrogen) ...
-
bioRxiv - Neuroscience 2020Quote: ... Digestion products were resolved in 3-8% tris-acetate SDS-PAGE followed and visualized by Zinc staining (Life Technologies). Images acquisition (Chemi-Doc Camera ...
-
bioRxiv - Neuroscience 2020Quote: ... primary DP cells from P5-8 Tgfbr2fl/fl molars were grown in alpha-minimum essential medium (alpha-MEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum ...
-
bioRxiv - Systems Biology 2021Quote: ... 75,000 cells were seeded into collagen-1 (Cultrex #3442-050-01) coated 8-well plates (Thermo Fisher Scientific 267062) in GM ...
-
bioRxiv - Bioengineering 2021Quote: A standard endodermal differentiation protocol calls for HUES-8 spheroids to be cultured in MCDB131 medium (Thermo Fisher Scientific) containing 200 ng/mL Nodal or 100 ng/mL Activin A (both obtained from R&D Systems ...
-
bioRxiv - Biochemistry 2020Quote: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed with 3-8% Tris-Acetate gels (Invitrogen, Carlsbad, CA), as described previously [27] ...
-
bioRxiv - Biochemistry 2020Quote: ... and 8 pmol of purified DNA was then used for in vitro transcription with T7 TranscriptAid kits (Thermo Scientific). The resulting RNA was purified with Agencourt RNAClean XP beads supplemented with an additional 12% of PEG-8000 and quantified via NanoDrop ...
-
bioRxiv - Cell Biology 2021Quote: ... Beads were washed extensively (8 times) in extraction buffer before being resuspended in 1 ml of Trizol (Invitrogen, 15596026) and vortexed vigorously ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
-
bioRxiv - Bioengineering 2021Quote: The tissue was washed with 40 μl 1X RT buffer containing 8 μl Maxima 5x RT Buffer (EP0751, Thermofisher), 1 μl RNase Inhibitor (30281 ...
-
bioRxiv - Microbiology 2020Quote: Human ileocecal adenocarcinoma cells (HCT-8 cells, ATCC CCL-244) were maintained in RPMI 1640 medium (Gibco, ATCC modification) supplemented with 10% fetal bovine serum ...
-
bioRxiv - Microbiology 2020Quote: Cells seeded into 8-chamber culture slides (Nunc™ Lab-Tek™ II Chamber Slide™ System, ThermoFisher Scientific) (0.4×106 cells/well ...
-
bioRxiv - Microbiology 2021Quote: ... A total of 20 μg of whole cell lysate were resolved on a 3-8% Tris-Acetate NuPAGE (Invitrogen) and analyzed by immunoblotting with anti-SPTBN1 ...
-
bioRxiv - Immunology 2020Quote: ... following SDS-PAGE in reducing and non-reducing conditions using NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies). Purified proteins were dialyzed overnight against PBS using Slide-A-Lyzer® dialysis cassettes (10 kDa MW cut-off ...
-
bioRxiv - Microbiology 2020Quote: ... BS-C-1 cells were seeded into Nunc™ 8-well Lab-Tek II chambered coverglass slides (Thermo Scientific) to obtain 50 to 70% confluence on the day of tracking ...
-
bioRxiv - Cell Biology 2021Quote: WA09 (H9) hESC line was purchased from WiCell (wicell.org) and maintained in Essential 8 medium (A151700, Thermo Fisher Scientific) on hESC-qualified growth factor reduced Geltrex-coated (A1413302 ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were passaged (0.05% Trypsin EDTA, #25300–054, Gibco, Waltham, MA and 5mg/mL soybean Trypsin inhibitor, #9035-81-8, Gibco) and seeded at 4,000 cells/cm2 into 1× 60 mm and 1× 35 mm collagen-coated culture dishes ...
-
bioRxiv - Microbiology 2022Quote: ... pH 8) at room temperature in PierceTM Slide A Lyzer® Mini Dialysis Units (Thermo Fisher Scientific, Waltham, MA). Turbidity measurements at A340 nm were collected in order to quantify the reaction progress by using NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
-
bioRxiv - Biophysics 2022Quote: ... at 37°C and 8% CO2 to a density of 3.5×106 cells/mL in FreeStyle suspension media (Gibco) supplemented with 2% fetal bovine serum (Gibco ...
-
bioRxiv - Cell Biology 2022Quote: ... 10 μl of the supernatant was used for SDS-PAGE on 8-12% Tris-Glycine gels (NOVEX™, Invitrogen) with Tris-Glycine SDS Running buffer (NOVEX™ ...
-
bioRxiv - Cell Biology 2022Quote: ... livers were perfused in situ with Hank’s balanced salt solution without calcium containing 8 mM HEPES (Thermo Fisher Scientific) via the inferior vena cava after cutting the portal vein to allow outflow of the perfusate ...
-
bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
-
The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: DRG cultures were prepared as described with the neurons plated on 8-well Chamber Slides (154534; Nunc Lab-Tek) coated with poly-D-lysine (P0899 ...
-
bioRxiv - Neuroscience 2021Quote: ... HeLa digest was fractionated into 8 fractions using a high pH reversed-phase peptide fractionation Kit (ThermoFisher, Cat. #84868). Twenty μg of HeLa digest was loaded to a C18 solid phase spin column and eluted with a serial concentration of ACN (5% to 50%) ...
-
bioRxiv - Plant Biology 2021Quote: ... and PpEF1α were cloned into pCR™8/GW/TOPO™ or pCR™-Blunt (Thermo Fisher Scientific, Inc.). To analyze virulence and protein accumulation in N ...
-
bioRxiv - Neuroscience 2020Quote: ... The individual colonies were picked to 24-well matrigel-coated plate containing Essential 8 Medium (E8, Thermo Fisher Scientific) between days 17-28 and passaged with 0.5 mM EDTA weekly ...
-
bioRxiv - Neuroscience 2020Quote: ... of brain grey matter tissue was homogenized in 500 μL of 8 M urea lysis buffer (8 M urea, 100 mM Na2HPO4, pH 8.5) with HALT protease and phosphatase inhibitor cocktail (ThermoFisher) using a Bullet Blender (NextAdvance) ...
-
bioRxiv - Neuroscience 2021Quote: A solution of 2 μM of Calcein-AM and 8 μM of ethidium homodimer-1 (both from L3224, ThermoFisher) in DPBS (14190-144 ...
-
bioRxiv - Microbiology 2022Quote: Between 7×104 and 1.2×105 HepG2 cells (ATCC) per well were seeded in an 8-well Lab-Tek chamber slide (Nunc), precoated with rat tail collagen type I (Sigma ...
-
bioRxiv - Neuroscience 2022Quote: ... 2010) as follows: protein samples were separated in NuPAGE™ 3-8% Tris-Acetate Protein Gels (Thermo Fisher EA03755) in Tris-Acetate-EDTA running buffer (Thermo Fisher BP13354 ...
-
bioRxiv - Genetics 2022Quote: ... Cells were pelleted at 3,500 x g for 8 minutes and the pellet resuspended in 1 ml of Trizol (Invitrogen) and incubated for 5 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... 20μg of each respective sample was loaded onto a 1mm 3-8% Tris-Acetate Mini Protein gel (NuPAGE) along with 10μl of Spectra Multicolor High Range protein ladder (Thermofisher). The gel was run at 80V for 15 minutes and then increased to 120V until the dye front reached the bottom ...
-
bioRxiv - Microbiology 2022Quote: ... interferon-β treated (250 U/ml for 8 h) or infected with influenza WSN (MOI 0.02 for 24 h) using TRIzol (Invitrogen). Biologic triplicate sample RNA was sent for library preparation and paired-end RNA-seq by Novogene (BioProject PRJNA667475) ...
-
bioRxiv - Neuroscience 2019Quote: Single-molecule FRET measurements were carried out using ~30 pM of labelled tau in phosphate buffer (40 mM potassium phosphate, 50 mM KCl, pH 7.4) in 8-chambered Nunc coverslips (ThermoFisher) passivated with poly(ethylene glycol ...