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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and primary antibodies for 3 days: chicken anti-GFP (1:750, Thermo Fisher Scientific Cat# A10262 ...
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bioRxiv - Cell Biology 2023Quote: ... 0.05% Tween-20 in PBS along with 1:1000 To-Pro-3 (Invitrogen) for 2h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... species-specific secondary antibodies (Life Technologies; 1:300 dilution in 3% BSA/PBS). Images were collected on a Zeiss Axioimager.D2 upright microscope using a 63x/Plan-neofluar objective and captured using a Coolsnap HQ2 camera (Photometrics ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 U/ml EPO (Zyrop 4000 IU injection) and 1× Pen-Strep (Gibco) as per established protocols ...
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bioRxiv - Biophysics 2023Quote: ... NucGreen™ Dead 488 (Invitrogen™, 1 drop per 3 ml in PBS); Alexa 647 Phalloidin (Invitrogen™ at 1/500) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA and lipofectamine (1:3 w/v) were diluted in Opti-MEM (Gibco) and incubated at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
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The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... The embryos were immunostained with TO-PRO-3 (Thermo Fisher; T3605; 1:1000) to identify the nuclei and images were captured using an A1 confocal microscope (Nikon) ...
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bioRxiv - Cancer Biology 2024Quote: ... and sgRNAs at a 1:3 molar ratio in Neon buffer R (Invitrogen) and incubating at room temperature for 10 min ...
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bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... at 37°C with 5% CO2 with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1⍰% non-essential amino acids (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... were grown at 37°C in 5% CO2 with 1% Penicillin/Streptomycin (PS; 5000U.mL-1 and 5000µg.mL-1; Life Technologies) and supplemented with 1% non-essential amino acids (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... 1:200 in 1% PBSA and then incubated for 5 min with Syto9 (1:1000) ® (Thermo Scientific) for nuclear staining ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C with 5% CO2 with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies).
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bioRxiv - Plant Biology 2020Quote: ... 5 mg·mL-1 in acetone diluted 1:250 in Dulbecco’s phosphate-buffered saline (DPBS, ThermoFisher) and propidium iodide (PI ...
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bioRxiv - Genomics 2022Quote: ... media was changed to 1:1 KSR: N2B media with puromycin (5 ug/ml, Gibco). Puromycin was maintained in the media throughout the differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Biophysics 2024Quote: ... 5 μg·ml-1 blasticidin (MilliporeSigma, 203350) and 100 μg·ml-1 zeocin (Thermo Scientific Chemicals, J67140XF). Transfected cells were maintained in the complete culture medium ...
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bioRxiv - Cancer Biology 2023Quote: ... media was changed to 1:1 KSR: N2B media with puromycin (5 ug/ml, GIBCO). Puromycin was maintained in the media throughout the differentiation ...
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bioRxiv - Biophysics 2021Quote: ... The ligation was desalted by dialysis using membrane filters for 1h and later concentrated 3.3X using a SpeedVac concentrator (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were serum-starved for 1h by adding 1.5mL Opti-MEM® reduced-serum medium (Gibco, Life Technologies) per well prior to transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... Dissociated murine prostate cells were obtained by digesting pre-minced prostate tissue for 1h at 37°C in digestive medium prepared in prepared in ADMEM/F12 (Gibco), and containing 1mg/mL Collagenase Type I (ThermoFischer Scientific ...
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bioRxiv - Genomics 2020Quote: ... W4021) were added for 1h and protein signals were detected using Pierce™ ECL Western Blotting Substrate kit (ThermoFisher 32109), using a Fujifilm LAS4000 luminescence imager and band intensities quantified using ImageJ ...
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bioRxiv - Biochemistry 2020Quote: ... slides were rinsed with PBS and incubated for 1h with secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 568 anti-rat; Invitrogen) and DAPI (4083 ...
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bioRxiv - Microbiology 2020Quote: ... The non-encapsidated DNA was then removed by a 1h incubation at 37°C with 4 U TURBO DNase enzyme (Invitrogen), 1X TURBO DNase Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... washed thrice with PBST followed by 1h incubation with Alexa fluor conjugated secondary antibody (Thermo Fisher Scientific Cat # 11008, 21244) then again washed thrice with 1XPBST ...
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bioRxiv - Immunology 2022Quote: ... were generated by plastic adherence for 1h in complete RPMI 1640 (680 μM L-Glutamine and 10 mM Hepes, GIBCO) supplemented with 5% pooled human serum (The Blood Bank ...
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bioRxiv - Cell Biology 2021Quote: ... samples were incubated at room temperature for 1h with the corresponding fluorescence secondary antibodies conjugated to Alexa Fluor 488 or 555 (Invitrogen) at 1:400 ...
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bioRxiv - Bioengineering 2021Quote: ... Chips were then incubated at 37°C for ~1h until cells were fully dissociated into single cells at which point DTI (Gibco) was added to neutralize the enzymatic activity ...
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Activation of endoplasmic reticulum stress via clustering of the inner nuclear membrane protein SUN2bioRxiv - Cell Biology 2022Quote: ... were coated for 1h with 0.1% gelatin solution (ATCC, PCS-999-027) and washed twice with PBS (pH 7.4, Thermo Fisher Scientific). HEK293FT cells were plated on the gelatin coated dish and grown overnight in normal growth medium to reach ∼ 70% confluency ...
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bioRxiv - Immunology 2021Quote: from PyMT or wild-type littermate control were cut in small pieces and digested for 1h at 37°C in CO2-independent medium (Gibco) containing 150 μg/mL DNAse and 75 μg/mL Liberase TL (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were put back on ice and treated for 1h with anti-Alexa fluor® 488 (#A11094 from Life Technologies) in order to quench remaining surface signal ...
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were serum-starved for 1h by adding 1.5mL Opti-MEM® reduced-serum medium (Gibco, Life Technologies) per well prior to transfection ...
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bioRxiv - Biophysics 2020Quote: ... fixed cells are incubated 1h at room temperature with Myosin 4 Monoclonal Antibody conjugated with Alexa Fluor 488 (MF20, Thermofisher) at 5μg/mL (1:100 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were blocked in blocking buffer for 1h at room temperature and incubated with BODIPY 558/568 (Invitrogen, D38D35) for 20 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... and immunocomplexes were next incubated for 1h at 4°C with 40 μL of Dynabeads Protein A (Thermo Fisher Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated for 1h at room temperature in PBS- tween with 2.5% BSA and developed using ECL Western blot reagent (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were subsequently rinsed and incubated for 1h at RT with the appropriate HorseRadish-Peroxidase (HRP)-conjugated secondary antibodies (Invitrogen). The visualisation of the signal was conducted using Immobilon™ Forte Western HRP Substrate (Millipore ...
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bioRxiv - Immunology 2023Quote: ... 100 uL ofmedium from the bottom chamber were collected at either 1h or 4h after Dextran addiction and fluorescence was quantified (Emission; 490, Absorption; 520) on black 96 multiwell plates (Nunc) on CLARIOstar OPTIMA (BMG LabTech ...
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bioRxiv - Biophysics 2022Quote: ... The ligation was desalted by dialysis using membrane filters for 1h and later concentrated 3.3X using a SpeedVac concentrator (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... sections were boiled in sodium citrate buffer (pH 6.0) for 15 min and incubated with the following antibodies for 1h: anti-CD8 (4SM15, Thermo Scientific), anti-NCR1 (EPR23097-35 ...
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bioRxiv - Cell Biology 2024Quote: ... incubated for 1h at RT with secondary antibody (4 µg/ml goat anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific) in PBS-BSA ...
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bioRxiv - Pathology 2024Quote: ... The iPSCs were fed every other day and were passaged 1:5-10 every 5 days using Versene (Invitrogen). All the protocol was approved by the Medical Research Ethics Committee of Wenzhou Medical University (2017-066 ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mM EDTA, 5 mM MgCl2, 1% NP-40, 0.5 mM DTT, 1X protease and phosphatase inhibitor cocktail; ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed at room temperature for 2h with Alexa 488 anti-mouse IgG-conjugated antibodies (Molecular Probes Inc., Eugene, OR, USA). Cell nuclei were stained with DAPI (Molecular Probes) ...
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bioRxiv - Cancer Biology 2022Quote: ... with or without N-ethyl-N-nitrourea 10μg/ml or 50μg/ml and treated 2h with 1mg/ml Collagenase type IV (Gibco by life technologies ref 17104-019).
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bioRxiv - Cancer Biology 2021Quote: ... Drug dilution series (1:3) were prepared in PDAC organoid culture medium containing 1 μg/ml Hoechst (Invitrogen) and 1 μg/ml PI (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Plasmid DNA was mixed with PEI (1 mg/ml) at 1:3 in Opti-MEM medium (Gibco, 11058021) and added to cells after short incubation ...
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bioRxiv - Genetics 2022Quote: Calu-3 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM)/F12 (1:1) GlutaMAX™ (ThermoFisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the tumoroids in a 1:3 and 1:10 effector:target (E:T) ratio in RPMI 1640 (Gibco) supplemented with 10% FBS ...