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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Cell Biology 2023Quote: Sac2 knockdown was performed with siRNA targeting mouse gene sequence Inpp5f: 5’-GGAAUGCGGUAUAAACGAATT-3’ and was from Ambion (Life Technologies). OSBP knockdown was performed using ON-TARGET plus SMART pool Mouse OSBP siRNA from Dharmacon ...
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bioRxiv - Neuroscience 2023Quote: ... washed in PBS (3 x 5 min) mounted on glass slides (SuperFrost Plus Microscope Slides, ThermoFisher Scientific, Waltham, MA, USA) and left to air-dry overnight ...
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bioRxiv - Zoology 2023Quote: ... the sections were again washed with TBS (3 times; 5 min each) and mounted in Fluromount-G mounting media with DAPI (004959-52, Invitrogen). The dried slides were visualized using Leica DM4000B fluorescence microscope equipped with Leica DFC310 FX camera ...
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bioRxiv - Developmental Biology 2023Quote: ... was synthesized on templates that contain the T7 promoter sequence (5’-TAATACGACTCACTATAGGGTACT-3’) at each end using a MEGAscript T7 kit (Ambion); templates were amplified from 0–4 h embryonic cDNA using specific primers (5’-AGCAGATGACGACGACAACA-3 ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg of antibody (3 µg for H3K27ac) was added to 5 µg of sonicated chromatin along with Dynabeads Protein A magnetic beads (Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed 3 times for 5 minutes each with PBS and mounted using ProLong Diamond anti-fade mountant with DAPI (Invitrogen) and allowed to cure overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 5′-CCUGGAUAAUGAUGAAGGA-3′), OSBP (predesigned, cat. no. 4392420) and nontargeting control siRNA (predesigned, cat. no. 4390844) were purchased from Ambion. ON-TARGET plus Human PI4K2A siRNA (predesigned ...
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bioRxiv - Cell Biology 2022Quote: ... All samples were boiled for 5 min prior to separation by SDS PAGE on precast 3-8% Tris-Acetate NuPAGE gels (Invitrogen). SDS-PAGE fractionated proteins were transferred to a nitrocellulose membrane using a semidry Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Physiology 2022Quote: ... R:3’-UUGAACGUCACUAUAUUAACUGUUGUA-5’) dicer-substrate siRNA (DsiRNA) (20 nM, Integrated DNA Technologies, Coralville, IA) using Lipofectamine 3000 transfection reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant was clarified and resuspended in PBS (3 mM EDTA) with 5 μg/mL Hoechst (33342 Thermo Fisher Scientific) and incubate 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... and probe 5’-FAM-TCCAGCCTCCATAGCCGGGAAGG-TAMRA-3’ were used in a 25μL reaction with Supermix Platinum™ Quantitative PCR SuperMix-UDG (Invitrogen). The reaction was performed in a 96-well format for real time quantification on Applied Biosystems 7900HT Fast Real-Time PCR System ...
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bioRxiv - Systems Biology 2023Quote: ... labeled with secondary antibodies (2 hrs at RT) and washed again in PBS (3 x 5 min at RT) before being mounted in Prolong Gold (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... For the construction of the RNH2A KO clones, RNASEH2A (Chr19, exon 2) gRNA (5’-TAACAGATGGCGTAGACCAT-3’) was cloned into GeneArtTM CRISPR Nuclease Vector with OFP reporter (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were washed with PBS 3 times for 5 minutes and Mili-Q water once before being mounted on slides by Profade-Antifade (P36935, Invitrogen) overnight in dark ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed 3 times for 5 minutes with PBS and then incubated with secondary antibodies (Alexa fluorophores 488 and 555, Invitrogen) for 1 hour in dark at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... siLuc (5′-CGUACGCGGAAUACUUCGAUUdTdT-3′) and siARP3 (SMARTpool siRNA L-012077-00-0010 (Dharmacon)) were transfected using RNAiMax (Thermo Fisher Scientific) according to manufacturer’s instruction.
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bioRxiv - Molecular Biology 2023Quote: ... Takara)-coated plates at an MOI of 5 and the transduced population was enriched by puromycin (3 µg/mL; Gibco, China) selection for 5 days ...
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bioRxiv - Pathology 2024Quote: ... right arm target 5’-ACA TCG CTA ACC TGC TG CT-3’) were synthesized using the mMESSAGE mMACHINE kit (Ambion) and injected into one-cell stage embryos at 100 pg per embryo ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were dissociated using 0.05% Trypsin for 5 min at room temperature and subjected to 3 washes with Hanks’ Balanced Salt Solution (HBSS, Gibco). Cell supernatants were triturated in DMEM media with 20% fetal bovine serum using a 1000 μL pipette and applied to a 100 μm cell strainer (MidSci ...
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bioRxiv - Cancer Biology 2024Quote: ... Human lung fibroblast MRC5 and its SV40-transformed derivatives were cultured in a 5% CO2 and 3% O2-regulated incubator in MEM medium without Phenol Red (Gibco), completed with 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: Ribosome footprints were prepared from the youngest fully expanded leaf from 5 week old tobacco plants or from 3-week old Arabidopsis seedlings using Ambion RNase I (Invitrogen) as described previously (Chotewutmontri et al. ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 80–100 pairs of ovaries from 3- to 5-day-old flies (for the analysis of piRNA production in somatic follicle cells) using TRIzol Reagent (Ambion). After 2S rRNA depletion ...
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bioRxiv - Molecular Biology 2024Quote: ... The siRNA sequences targeting human TFEB (5□-GAA AGG AGA CGA AGG UUC AAC AUC A-3□) were purchased from Invitrogen. The siRNA sequences targeting human NPC1 (5□- CAA UUG UGA UAG CAA UAU UTT-3□ ...
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bioRxiv - Microbiology 2024Quote: ... was cultured at a density of 3 × 10^5 cells/ml in RPMI-1640 medium (Roswell Park Memorial Institute Medium, Gibco), supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: The cell line containing the Cre-Blasticidin and the CMV-GFP cassettes were cultured for 3-5 days in IMDM media (Gibco, with 10% FBS and 1% Penicilin/Streptomycin ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed 3 times with imaging buffer and incubated in imaging buffer containing 5 μM EGTA-AM (#E1219, Invitrogen). After 45 min of incubation ...
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bioRxiv - Genomics 2024Quote: ... Media was changed daily and 3×10^5 cells were passaged every other day using TryplE (Life technologies, Cat.N: 12604013) with a 30-minute selective sedimentation step where the detached cells were kept in an uncoated 10cm dish at 37C to separate ES from MEFs ...
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bioRxiv - Immunology 2024Quote: ... Excess secondary antibodies were washed off with PBS (3 washes, 5 minutes each) followed by addition of 32.4 μM of Hoechst nuclear staining reagent (ThermoFisher #H3570) to label nuclei ...
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bioRxiv - Neuroscience 2024Quote: ... Thereafter, sections were rinsed as before (3 x 5 min, cold TBS) then incubated with AlexaFluor conjugated secondary antibodies (Thermofisher) in 5% normal goat serum in TBS for 2 h at 4°C with agitation ...
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bioRxiv - Neuroscience 2024Quote: ... and Biotinylated Dextran Amine (BDA, cases AV, AT: equal parts 10% 10 kDA and 10% 3 kDA, 5 µl; Invitrogen). Dextran amines with molecular weight 10 kDA (BDA in AT and AV ...
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bioRxiv - Neuroscience 2024Quote: Duplex of the MEF2C enhancer sequence containing a mutation site [5′-ATGTATTTTTCTGCAATAAGT-3′ (×2)] in human genomic DNA were synthesized (Invitrogen). Additionally ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed 3 times with 1X PBS for 5 minutes each and incubated with secondary antibodies Alexa Fluor 633 (A21094, Thermofisher), Alexa Fluor 555 (A21422 ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µl sample was applied to glow-discharged grids and incubated for 5 s followed by blotting for 3 sec using a Vitrobot Mark IV (FEI ThermoFisher) operated at 4 °C and 100% humidity ...
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bioRxiv - Genomics 2024Quote: Antibodies were obtained in >50 ug quantities and labeled with bifunctional 5’ Acrydite - bit sequence - 3’ DBCO oligos (IDT) by enzymatic modification and click chemistry (SiteClick Antibody Azido Modification Kit, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: siRNA oligonucleotides targeting DEK (5’-GAAGGCTAAGCGAACCAA A-3’) at a final concentration of 50 nM were transfected into cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... LNCaP cells were seeded at 80% confluence and grown for 3 days in phenol red-free RPMI Medium with 5% charcoal-stripped Fetal Bovine Serum (FBS; #cat 12676029, Gibco) and then stimulated with 10 nM 5-α-dihydrotestosterone (DHT ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was washed 3 x 5 min with PBS and thereafter mounted with Prolong Diamond Antiface Mountant (ThermoFisher, P36961). The tissue samples were placed in the dark at RT for at least 1 day before imaging.
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bioRxiv - Genetics 2024Quote: The resulting cDNA was amplified with primers SD6-PSPL3_RT-FW (5’-TCACCTGGACAACCTCAAAG-3’) and RTpSAD-RV (CSIC Patent P201231427) using Platinum Taq DNA polymerase (Life Technologies) and the following cycling conditions ...
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 times for 5 min with 1x PBS before mounting onto glass slides using ProLong Diamond Antifade mountant (Invitrogen) and left to dry overnight at 4°C before imaging.
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was incubated 2h at 37°C and then loaded on a 15% acrylamide/8M urea precast gel (Invitrogen, cat n° EC6885BOX). The ligated RNA was purified through gel extraction ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed three times with PBS/0.025% Triton X-100 before incubation (room temperature for 2h) with the following secondary antibodies: anti-mouse coupled to Alexafluor 555 (A31570, Invitrogen, Waltham, MA, USA), anti-rabbit coupled to Alexafluor 488 (A21206 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were washed in 3 times in 3% PBST (10 min) and incubated in goat anti-chicken Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in blocking buffer at room temperature for 2hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Genetics 2021Quote: ... Quality and quantity of the RNA were measured using the NanoDrop-1000 spectrophotometer (Thermo Scientific, Wilmington DE, USA).
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bioRxiv - Genetics 2021Quote: ... Quality and quantity of the RNA were measured using the NanoDrop-1000 spectrophotometer (Thermo Scientific, Wilmington DE, USA) and RNA integrity was determined by agarose gel electrophoresis (3 μL of sample RNA on 1% agarose gel).
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bioRxiv - Genetics 2020Quote: ... following de-paraffinization the slides were incubated in eBioscience IHC Antigen Retrieval Solution (ThermoFisher; Cat# 00-4955-58) and washed in 1x PBS three times for five minutes ...