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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: The concentration of all RNA samples was measured on a Nanodrop 2000/2000c (Thermo Fisher Scientific, Wilmington, DE, USA). mRNA from each time point was sequenced using Illumina HiSeq platform at an average of 85 M reads per library (Novogene ...
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bioRxiv - Evolutionary Biology 2020Quote: The concentration of all RNA samples was measured on a Nanodrop 2000/2000c (Thermo Fisher Scientific, Wilmington, DE, USA). Dried in samples where shipped on dry ice to Novogene (Hong Kong ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was resuspended in nuclease-free water and quantified using a spectrophotometer (NanoDrop, Thermo Fisher Scientific, Wilmington, DE, USA). RNA (1 µg ...
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bioRxiv - Genomics 2020Quote: ... and RNA concentration was determined by UV-Vis spectrophotometry using a Nanodrop™ 2000 (Thermo Fisher Scientific, Wilmington, DE). Complementary RNA (cRNA ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was isolated from the supernatant and quantified using the NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, United States), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... expression vectors were constructed by inserting de novo synthesized (Geneart) codon optimized HC coding regions into expression vector pcDNA3.3 (Invitrogen). The HC coding regions consisted of the VH regions of mAbs 7D8 (human CD20-specific [Teeling et al. ...
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bioRxiv - Genomics 2023Quote: ... DNA purity was determined using a NanoDrop 1000 spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA) and quantified using the Qubit 2.0 Fluorometric Quantitation (Invitrogen, Life technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was quantified using a nano-drop spectrophotometer (NanoDropTM 2000/2000 c, Thermo Fisher Scientific, DE, United States). High throughput genotyping was carried out using Illumina rice 7K Chip ...
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bioRxiv - Microbiology 2023Quote: ... The quantity and quality of DNA extracts were confirmed using NanoDrop spectrophotometer ND-1000 (Thermo Fisher Scientific, Wilmington, DE). All DNA extracts were stored at –80°C.
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bioRxiv - Developmental Biology 2022Quote: ... The digestion was terminated with termination buffer containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Wilmington, DE, USA) and the samples were filtered through a 40-μm filter and centrifuged ...
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bioRxiv - Synthetic Biology 2022Quote: ... The content of protein in the samples was determined with the A280 Total Protein Assay (Thermo Scientific, Wilmington, DE). A volume equivalent to 100 μg protein per sample was taken through a modified filter-aided sample preparation (FASP ...
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bioRxiv - Cancer Biology 2023Quote: ... T-47D cells were kindly donated by Dr Miquel Angel Pujana (ProCure Program, Institut Català d’Oncologia, Hospitalet de Llobregat, Spain) and were grown with RPMI Medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Genomics 2024Quote: ... The genomic DNA purity and quantity were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE) and a Qubit 2.0 fluorometer (Life Technologies Ltd. ...
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bioRxiv - Molecular Biology 2020Quote: ... Beas-2B cells were cultured at 37°C in a humidified atmosphere of 5% in serum-free 1× defined keratinocyte SFM Gibco) supplemented with 5 µg/mL gentamicin (Gibco). The medium was replaced every 2–3 days and cells were passaged every 4–5 days ...
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bioRxiv - Molecular Biology 2020Quote: ... Total RNA (5 µg) was combined with ERCC Spike-In Standards (5 µl of 1:50 diluted stock solution; Invitrogen) and submitted to the University of Minnesota Genomics Center for library generation and sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... to a final concentration of 5% and fetal bovine serum to a final concentration of 5% and 1% penicillin-streptomycin (Invitrogen) or Clear X9-Stem™ at 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: Whole proteome and phosphopeptide samples were dissolved in 2% [v/v] ACN 0.1% [v/v] TFA and injected onto a C18 PepMap100-trapping column (0.3 x 5 mm, 5 μm, Thermo Scientific) connected to an in-house packed C18 analytical column (75 μm x 300 mm ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained at 37°C with 5% CO2 in DMEM-F12 + 5% HI FBS + 1% Anti-anti (Gibco) + 1% MEM NEAA + 1% L-Glutamine.
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bioRxiv - Microbiology 2020Quote: ... 20 μl RNase A/T1 mix (2 μg μl−1 / 5 U μl−1, Thermo Scientific) was added to 100 μl lysate (OD260 ~ 150 ...
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bioRxiv - Biophysics 2021Quote: ... made of 5:1:1 ratio of H2O : H2O2 (50 wt. % in H2O, stabilized, Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... made of 5:1:1 ratio of H2O: H2O2 (50 wt. % in H2O, stabilized, Fisher Scientific): NH4OH (ACS reagent ...
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bioRxiv - Cell Biology 2020Quote: ... made of 5:1:1 ratio of H2O: H2O2 (50 wt. % in H2O, stabilized, Fisher Scientific): NH4OH (ACS reagent ...
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bioRxiv - Cell Biology 2021Quote: ... made of 5:1:1 ratio of H2O : H2O2 (50 wt. % in H2O, stabilized, Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... Antibodies were NKX2-5 (mouse, R&D, 1:500) and β -actin (rabbit, Invitrogen, 1:1000). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... the media was changed to 1:1 KSR: N2B media with puromycin (5 μg/ml, Gibco), where N2B was composed of DMEM/F12 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary GR antibody (G-5, Santacruz sc-393232, 1:100) or MAP2 antibody (Invitrogen, 1:2000) incubations lasted for 1 h at RT followed by 3x washes and 1 h incubation in secondary antibody at RT (Cy3 Goat anti-mouse against GR ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Cell Biology 2023Quote: ... centrifuged at 1,000 rpm for 5 min and resuspended in DMEM:F-12 (1:1) (Gibco, C11330500BT) media supplemented with 10% FBS and 1% penicillin- streptomycin ...
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bioRxiv - Genomics 2020Quote: ... Transformed cells were recovered in S.O.C media (from ElectroMAX Stabl4 kit) for 1h and spread on Luria–Bertani agar plates (245×245 mm, Thermo Fisher Scientific) containing 100 µg/mL ampicillin ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by washing with PBS three times and incubating for 1h at RT in blocking buffer containing Alexa-Fluor-conjugated isotype-specific secondary antibodies (Thermo Fisher). Cells were washed three times with PBS and DAPI (0.4 μg/ml in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and 10-20 µg of protein were loaded into 4-12% Bis-Tris Bolt gel and run at 150-200V constant for 1h in Bolt MOPS/MES 1X running buffer (Thermo Scientific). Dry transfer of the proteins to a nitrocellulose membrane was done using IBLOT2 (Thermo Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... 6d ovaries underwent 1h of EdU labelling based on the protocol of the Click-iT™ Plus EdU Cell Proliferation Kit for Imaging (Invitrogen). Both 6d and 12d ovaries were stained for Fasciclin III (Fas3 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were allowed to adhere for 4 hours (+/-1h) before transfection with individual siRNAs (10nM) diluted in a mix of OptiMEM (Life Technologies) and 0.05μL of Interferin reagent (Polyplus Transfection) ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat antibody conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/500 in PBS ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed and incubated for 1h at room temperature with horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Invitrogen #A18805), goat anti-human IgM (Invitrogen #A18835) ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies/stains were diluted in 2% BSA PBS and samples were incubated for 1h and coverslips were subsequently washed and mounted with ProLong Gold anti-Fade moutant (Thermofisher, P36934). Localisations were visualised on a Ti-E Nikon microscope or Leica SP5 Confocal.
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bioRxiv - Genetics 2021Quote: ... Peptides were resuspended in 0.1% FA and were separated using a 1h gradient on an nLC coupled to a Q Exactive Plus tandem mass spectrometer (Thermo scientific) or an LTQ orbitrap XL mass spectrometer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... 1h in Towbin buffer (25mM Tris, 192mM glycine) with 20% methanol) in a wet tank system (Mini Blot Module, Thermo Fisher). The membranes were blocked in 5% dry milk powder in TBS-T (5mM Tris ...
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bioRxiv - Developmental Biology 2020Quote: ... 6d ovaries underwent 1h of EdU labelling based on the protocol of the Click-iT™ Plus EdU Cell Proliferation Kit for Imaging (Invitrogen). Both 6d and 12d ovaries were stained for Fasciclin III (Fas3 ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated for 1h with anti-human IgG goat polyclonal antibodies conjugated to FITC or Alexa Fluor 488 (H10301; A-11013, Invitrogen, USA) diluted 1/100 in FACS buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated 1h at RT in the dark with a secondary antibody solution containing goat anti-mouse Alexa 594 (A11032, Invitrogen, Fisher Scientific) fluorescent-coupled antibody diluted to 1:1000 in PBS-T solution ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 in blocking buffer for 1h and counterstained with DAPI prior to mounting with ProLong Gold anti-fade reagent (Invitrogen, P36930) for subsequent immunofluorescence analysis.
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with peroxidase-linked secondary antibodies for 1h at RT diluted in blocking buffer and then treated with Supersignal West Pico solution (Thermo Scientific). Images were acquired on an LAS-3000 system (Fuji Film).
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bioRxiv - Microbiology 2023Quote: ... diluted at 1:250 in blocking solution was incubated 1h at room temperature followed by a 45min incubation of a goat anti-rabbit Alexa-488 secondary antibody (Invitrogen, #A11034) diluted to 1:500 together with Hoechst and rhodamine-phalloidin ...
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bioRxiv - Neuroscience 2022Quote: ... The gel was fixed in 40% methanol and 10% acetic acid and subsequently stained for 1h using colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Fisher). Each lane from the gel was cut in 3 gel pieces and placed in 0.5-mL tubes ...
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bioRxiv - Neuroscience 2023Quote: Lumbar spinal cord and hippocampal sections were rinsed in 0.1M PB and subsequently incubated at room temperature for 1h with 30% (v/v) normal goat serum (Invitrogen, Cat#31872) in 0.1M PB containing 10% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... with 63x immersion objective magnification after 1h incubation with secondary antibodies (goat anti-mouse IgG 546 (Molecular Probes, Cat. no. A11030) and goat anti-rabbit IgG 488 (Molecular Probes ...