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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... integration-free vectors encoding for the Yamanaka’s genes (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) were used to transduce PBMCs ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were centrifuged at 200× g for 10 min and cultured in 24 well-plates in StemPro-34 complete medium (Thermo Fisher Scientific) supplemented with 200 mM GlutaMAX (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: Membranes were exposed to SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... then separated on a reverse-phase nano-spray column (2 μm, 75 mm i.d. × 50 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) using gradient elution ...
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bioRxiv - Neuroscience 2024Quote: ... and immersion fixed in 4% paraformaldehyde (PFA, Thermo Scientific) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: Zebrafish larvae at an age of 4 dpf were mounted in 1.5 % low melting point agarose (Thermo Fisher Scientific, Waltham, USA) and covered with E3 ...
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bioRxiv - Neuroscience 2024Quote: ... LC-MS analysis was performed using an Orbitrap Exploris 480 mass spectrometer equipped with FAIMS and interfaced to an Easy nanoLC1200 system (Thermo Fisher Scientific Cat# LC140). Peptides were loaded onto a C18 pre-column (3 μm ...
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bioRxiv - Biophysics 2024Quote: ... DAPI dilactate and YO-PROTM-1 Iodide was obtained from ThermoFisher Scientific (USA).
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bioRxiv - Neuroscience 2024Quote: ... PCR products were cloned into the pCRII-TOPO vector (Thermo Fisher Scientific, Waltham, USA). Resulting plasmids cpe_Ribo2 (pHD18 ...
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bioRxiv - Neuroscience 2024Quote: ... incubated 1 hour at room temperature in 1:500 Alexa Fluor-conjugated antibody (Invitrogen, Alexa Fluor 568 #A10042 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated for 10 min at room temperature in 1:5000 DAPI (Thermo Fisher, D1306) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then probed with anti-p75NTR (1:200; rabbit monoclonal, kindly gifted by P. Barker) and anti-GAPDH 1:8000 (mouse monoclonal IgG; Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the poly-HRP Streptavidin (Thermo Fisher, Cat# N200) was diluted at 1:8000 in 0.01M PBS-0.05% Tween ...
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bioRxiv - Biophysics 2024Quote: ... L-glutamine (catalog no. 25030024) was obtained from Thermo Scientific. Penicillin-streptomycin (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were counterstained with Hoechst 33342 (Invitrogen, H3570, 1:10,000) and mounted onto slides using Fluoromount-G (SouthernBiotech).
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bioRxiv - Neuroscience 2024Quote: ... and 647 (1:400, Life technologies; 1:1000, Cell Signaling Technology). After rinsing in PBS (three times) ...
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bioRxiv - Neuroscience 2024Quote: ... used for nNOS immunolabeling, and 568-conjugated donkey anti-rat secondary antibody (1:500; Cat#?, Lot#) used for GnRH were purchased from Invitrogen.
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bioRxiv - Neuroscience 2024Quote: ... sections were dehydrated via successive incubations in 95% ethanol and 100% ethanol and mounted onto glass slides with Permount (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... followed by a goat anti-mouse (1:3000, Invitrogen, Cat.62-6520, RRID:AB_2533947) or donkey anti-rabbit IgG (1:5000 ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentration was determined using Pierce BCA Protein Assay Kit (Cat No. 23325, ThermoFisher) according to the manufacturer’s manual.
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 488 or 568 (1:300 dilution) (Life Technologies), for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... stearic acid (10002390, Fisher Scientific), oleic acid (W281506 ...
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bioRxiv - Neuroscience 2024Quote: ... lifted with Accutase (Gibco), and plated at 375,000 cells/well of a 6-well plate onto Geltrex-coated plates (DIV0) ...
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bioRxiv - Neuroscience 2024Quote: ... Approximately 1 μg of RNA per sample (10.6 µL) was incubated with 2 µL TURBO DNase (Thermo Fisher Scientific) and 1.4 µL TURBO DNase buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... DNA extractions were performed with a kit (F140 WH, Thermo Scientific), after which Cre-recombinase was amplified by PCR ...
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bioRxiv - Neuroscience 2024Quote: ... polyclonal rabbit anti-Tau-phosphoSer422 (pSer422) (1:75 dilution) (Life Technologies, catalog no. 44-764G), monoclonal mouse anti-6H4 (1:1000 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... or Tri-IPC was sorted using a BigFoot Spectral Cell Sorter (Thermo Fisher) via single-cell precision mode into a single well of 96-well glass-bottom plates pre-coated with polyethylenimine and laminin containing 100 μL complete culture medium ...
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bioRxiv - Neuroscience 2024Quote: ... blocked with 10 % NDS-PB-TX and incubated in the dark in secondary fluorescently tagged anti-mouse antibody for either TH or GAD1 (1:200, MAB5406, EMD Milipore)) and fluorescently tagged anti-rabbit antibody for tracer (1:200, AlexaFluor, Invitrogen), overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... 10 mM NaCl, 3 mM MgCl2, 1 mM dithiothreitol, 1% BSA, 0.1% Tween 20, and 0.6 U/mL RiboLock [Thermo Fisher]). Next ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were used at 1:200 concentration (Life Technologies).
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bioRxiv - Neuroscience 2024Quote: ... resuspending in staining buffer containing Sytox Blue (Invitrogen), and sorted using BD FACSAria II sorters ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in 1X wash buffer (ACD Bio) and coverslipped using Prolong Gold anti-fade mounting media (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... and then in placed in solutions with primary antibodies FR (1:1000, Invitrogen #A6397, made in rabbit), FS (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was incubated in 3% NDS-PB-TX primary antisera to the tracer--either FR (1:1000, Invitrogen #A6397, made in rabbit), FS (1:500 ...
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bioRxiv - Systems Biology 2024Quote: ... 0.5 mL of TrypLE Express (Gibco, 12605010) was added to the suspension and incubated at 37°C for 10-15 minutes ...
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bioRxiv - Systems Biology 2024Quote: ... the cells were sorted using the Dynamag (Invitrogen) protocol ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with protease and phosphatase inhibitors without EDTA (Thermo Scientific #1861281). Protein concentration was determined by performing a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2024Quote: ... BTX ECM399 (Thermo Fisher Scientific) electroporator was used with 7 mm tweezer electrodes ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... and 36 μL Turbofect (Thermo Fisher) were mixed into 1.8 mL serum-free DMEM (Gibco) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... were mixed into 1.8 mL serum-free DMEM (Gibco), vortexed briefly ...
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bioRxiv - Systems Biology 2024Quote: The MS raw data was searched with Proteome Discoverer 2.1 software (Thermo Scientific, Waltham, MA) with Mascot 2.4 (Matrix Science ...
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bioRxiv - Systems Biology 2024Quote: ... a mixture of 50:50 carboxylate-modified Sera-Mag SpeedBeads type A and B (Cytiva) were washed three times with ddH2O (Barstead GenPure, Thermo Scientific) on a magnetic rack and 1 mg of combined beads were added to the E.coli sample ...
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bioRxiv - Systems Biology 2024Quote: Total RNA was extracted using Trizol reagents (Invitrogen Cat#15596026) as we have described before86 ...
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bioRxiv - Neuroscience 2024Quote: ... was applied for 10 minutes and the tissue sections were coverslipped using ProLong Antifade mounting medium containing DAPI (Thermo Scientific #P36935) to counterstain nuclei ...
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bioRxiv - Neuroscience 2024Quote: ... RNA concentration was determined using a Nanodrop (Thermo Fisher, Waltham, MA, USA). The cDNA was prepared using the Transcriptor High Fidelity cDNA Synthesis kit (Roche) ...
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bioRxiv - Systems Biology 2024Quote: ... 1x MEM non-essential amino acid solution (Gibco), 1mM sodium pyruvate (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2024Quote: ... and the concentration of total protein in cell extracts was determined using the Qubit® protein assay kit and the Qubit® 3.0 fluorometer (Thermo Fisher Scientific™). Cell culture supernatants were used purely ...
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bioRxiv - Systems Biology 2024Quote: ... We detected proteins by chemiluminescence using the SuperSignal® ELISA femto maximum sensitivity substrate (Thermo Fisher Scientific™, Waltham, USA) according to the manufacturer’s instructions and the Amersham™ Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Systems Biology 2024Quote: ... and HeLa digest standard (Thermo Fisher Scientific, Catalog number: 88328) were dissolved into different concentrations with 60% acetonitrile (ACN ...
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bioRxiv - Systems Biology 2024Quote: ... The entire assembly was connected by 20μm internal diameter Viper™ capillaries (Thermo Fisher Scientific) and kept at 55 °C in the column oven ...