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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 6 1 4 Dioxa 8 azaspiro 4.5 dec 8 yl 3 pyridinyl boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... and 3-6 μl Lipofectamin 2000 (Life Technologies). See the Supplemental Information for detailed description.
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bioRxiv - Immunology 2020Quote: ... and QuantStudio 3 or 6 Flex (ThermoFisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2021Quote: FBS free culture media with 15mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Gibco) in DMEM/F12 supplemented with 1% P/S was used for all experiments performed in the lung on a chip devices ...
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) 1M (ThermoFisher, ref. 15630106) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... and 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco, Gaithersburg, MD, USA). Cells were maintained at 37 °C in a humidified incubator with 5% CO2.
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bioRxiv - Biophysics 2024Quote: ... HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)) was obtained from Fisher Scientific (Pittsburg, PA). Peptides were reconstituted at 25 mg ml-1 in nuclease-free ultra pure water as per the manufacturer’s instructions and stored as aliquots at -20°.HP1α was reconstituted (0.5 mg ml-1 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... and 2-NDBG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] (N13195) were purchased from Invitrogen. Recombinant murine SCF (250-03) ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Microbiology 2020Quote: ... or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16, Thermo Fisher Scientific) in a modified SC medium ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Molecular Biology 2020Quote: ... after 3h of incubation was pelleted (3300 x g for 8 min, 4°C) and RNA was isolated using Trizol® Plus RNA Purification Kit (Ambion, Life Technologies, USA). The quality and quantity of RNA was determined by agarose gel electrophoresis and also using a NanoDrop ND-2000 spectrophotometer ...
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bioRxiv - Immunology 2021Quote: ... selected human splenic T cells from BLTS-humanized mice and human T cells from PBMCs were seeded at 8×10^4 cells per well (96-well plate) and stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Life Technologies Cat# 11131D) and PBS was used as vehicle control ...
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bioRxiv - Cell Biology 2022Quote: ... Hank’s balanced salt solution (HBSS Ca2+/ Mg 10X), BSA, sodium bicarbonate (NaHCO3) supplemented with 4% Paraformaldehyde (PFA, 40%, carl roth®) in 8-well plates (ThermoFisher Scientific, Cat # 177402). Cells were then washed twice and incubated 1h at RT in PFA (40% ...
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bioRxiv - Molecular Biology 2022Quote: ... CMCs were fixed for 5 min in 4% PFA in PBS and subsequently plated on laminin-coated 8-well Nunc™ Lab-Tek™ Chamber Slides (Thermo Fisher Scientific). CMCs were permeabilized and blocked for 1 hour in blocking solution containing 5% normal goat serum and 0.2% Triton X-100 in PBS after which sections were incubated with primary antibodies (see Key Resources Table ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were reconstituted in 0.1% trifluoroacetic acid and fractionated on Sep-Pak® Vac cartridges using methodology and reagents from Pierce™ High pH reversed-phase peptide fractionation kit (8 fractions for global proteomics and 4 for phosphoproteomics skipping every other; Thermo Fisher Cat No: 84868). Samples were run (1/8th of each global and 1/5th of each phosphopeptide fraction ...
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bioRxiv - Biophysics 2023Quote: ... cells were passaged 1:8 and placed in selection media—DMEM growth media containing 1 ug/mL puromycin (Thermo Fisher #A11138-03)—and selected for 7 days ...
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bioRxiv - Biophysics 2023Quote: ... and U-251 cells were seeded onto PLL-PEG-RGD-coated 8-well chambers in the respective medium with penicillin (1 unit/mL) and streptomycin (1 µg/mL; Gibco, Life Technologies) at densities between 0.5 x 104 to 2.5 x 104 cells/well ...
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bioRxiv - Systems Biology 2019Quote: ... the media was aspirated and replaced with 100μL phenol-red free RPMI-1640 and 12mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494) was added to the cells ...
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bioRxiv - Microbiology 2022Quote: Cell toxicity due to the compound treatment was determined using the standard colorimetric MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was measured by MTT assay using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide kit (Thermo-Fisher Scientific M6494). For the isobologram assays ...
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bioRxiv - Genetics 2022Quote: ... TO-PRO-3 Iodide carbocyanine monomer nucleic acid stain (1:1000, Thermo Fisher, cat# T3605) was used to stain DNA ...
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bioRxiv - Microbiology 2019Quote: 40 ng of RNA was amended with 8 μl of a 10,000X dilution of External RNA Control Consortium47 (ERCC) Spike-In Mix 1 (Ambion). The ERCC mix consists of 92 transcripts ranging from 250 to 2,000 nt in length with a large dynamic fold range ...
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bioRxiv - Systems Biology 2021Quote: ... 75,000 cells were seeded into collagen-1 (Cultrex #3442-050-01) coated 8-well plates (Thermo Fisher Scientific 267062) in GM ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were washed extensively (8 times) in extraction buffer before being resuspended in 1 ml of Trizol (Invitrogen, 15596026) and vortexed vigorously ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Microbiology 2020Quote: ... BS-C-1 cells were seeded into Nunc™ 8-well Lab-Tek II chambered coverglass slides (Thermo Scientific) to obtain 50 to 70% confluence on the day of tracking ...
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bioRxiv - Neuroscience 2021Quote: A solution of 2 μM of Calcein-AM and 8 μM of ethidium homodimer-1 (both from L3224, ThermoFisher) in DPBS (14190-144 ...
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bioRxiv - Genetics 2022Quote: ... Cells were pelleted at 3,500 x g for 8 minutes and the pellet resuspended in 1 ml of Trizol (Invitrogen) and incubated for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 1 × 104 293T-GRET/-PalmGRET cells were seeded per well into a glass-bottom 8-well chamber (#1.5, Nunc™ Lab-Tek™ II Chamber Slide™ System ...
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bioRxiv - Molecular Biology 2020Quote: BMDMs were seeded at 1×105 cells/well in 8 wells Nunc Lab-Tek II Chamber Slide (Thermo Scientific). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and plated onto coverslips (1:2 split) or Nunc 8 well chamber slides (Thermo Fisher # 155409PK) (20-40k cells per well).
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bioRxiv - Bioengineering 2023Quote: ... the cells were cultivated for 8 passages in expansion medium containing DMEM (gibco) supplemented with 1 % antibiotic– antimycotic (Invitrogen) and 10 % FBS (South Afrika FBS ...
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bioRxiv - Zoology 2024Quote: ... injected embryos were frequently cultured through stage 8 in HL saline containing roughly 1% commercial penicillin-streptomycin solution (Gibco).
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bioRxiv - Microbiology 2023Quote: ... The RNase T1 ladder was prepared by denaturing 0.4 pmol of 5′ labelled Aar with 8 µL of 1× sequencing buffer (Ambion) at 95°C for 1 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was replaced daily from 8 hpf and supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) to inhibit melanogenesis ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed 3 times for 8 minutes each and then placed in the secondary antibodies conjugated to either Cy3 or FITC (Thermo Fisher Scientific, Waltham, MA, USA) at a dilution of 1:1000 in 1% BSA for 2 hours ...
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bioRxiv - Genetics 2023Quote: Twenty μg protein samples were loaded into lanes of a 3-8% Tris-acetate gel (cat. EA0378BOX, NuPAGE™, Invitrogen, Thermo Fisher Scientific, CA, USA) and subjected to electrophoresis ...
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bioRxiv - Genetics 2023Quote: Twenty μg protein samples were loaded into lanes of a 3-8% Tris-acetate gel (cat. EA0378BOX, NuPAGE™, Invitrogen, Thermo Fisher Scientific, CA, USA) and subjected to electrophoresis ...
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bioRxiv - Neuroscience 2020Quote: ... 1 nl solution of 3 μM FM 4-64 (Molecular Probes, Invitrogen) dissolved in DMSO was injected in the otic cavity of 96 hpf zebrafish embryos mounted laterally in low gelling agarose (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 nl solution of 3 μM FM 4-64 (Molecular Probes, Invitrogen) dissolved in DMSO was injected in the otic cavity of 96 hpf zebrafish embryos mounted laterally in low gelling agarose (Sigma) ...
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bioRxiv - Synthetic Biology 2021Quote: ... samples were diluted 3:1 in 4× NuPAGE LDS sample buffer (Invitrogen) supplemented with 10% beta-mercaptoethanol (v/v) ...
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bioRxiv - Cell Biology 2022Quote: ... in 4:3:1 ratio into 293FT cells (Thermo Fisher Scientific, #R70007) with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... in a 4:3:1 mass ratio with Lipofectamine 2000 (Invitrogen # 11668019) in a 2:1 ratio of Lipofectamine 2000 ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µM 3-isobutyl-1-methylxanthine (Thermo Scientific, Cat # 28822-58-4), 1 µM dexamethasone (Thermo Scientific ...