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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1×106 cells were incubated with 5□μg/mL of Indo□1 AM (Invitrogen) and 0.5□μg/mL of pluronic F□127 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... media was changed to 1:1 KSR:N2B media with puromycin (5 μg/ml, Gibco). On day 3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 10% FBS (Cytiva HyClone) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were maintained in 5% CO2 and DMEM/F12 1:1 media (Gibco; Invitrogen) supplemented with 10% FBS (Cytiva HyClone) ...
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bioRxiv - Cell Biology 2021Quote: Cross-linking experiments of Sgo11-415 and CPCISB10-280 were performed using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Fisher Scientific) in the presence of N-hydroxysulfosuccinimide (NHS ...
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
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bioRxiv - Microbiology 2024Quote: ... Serum samples were assayed at 3-fold dilutions starting at a 1:3 dilution in Blocker Casein in PBS (Thermo Fisher Scientific) diluent ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by transfer to a positively charged nylon membrane and then crosslinked with EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) at 60°C for 1–2 hours and prehybridized with ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen, cat # AM8670). The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... HsTMEM120A was subjected to protease digestion with 1mg/ml proteinase K at 37°C for 1h (Thermo Scientific; EO0491). 0.2% SDS was added to the digestion solution to stimulate the activity of proteinase K ...
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bioRxiv - Microbiology 2022Quote: ... and incubated for 1h at RT with AlexaFluor488-conjugated donkey anti-mouse IgG secondary antibody (0.2 µg/ml; ThermoFisher) diluted PBS-HS ...
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bioRxiv - Biophysics 2022Quote: ... Samples were incubated for 1h with a filtered and sonicated solution of red fluorescent carboxylate-modified beads (FluoSpheres, Invitrogen) of 100nm (220nm filter ...
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bioRxiv - Neuroscience 2020Quote: ... the strips were incubated with goat anti-human IgG Fc HRP-conjugated secondary antibody for 1h (Thermo Fisher Scientific). The film was exposed following routine immunoblotting steps ...
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bioRxiv - Microbiology 2020Quote: ... for 1h at room temperature with gentle mixing followed by inactivation with 40U of SUPERase-In RNase inhibitor (Ambion). Cell extracts were passed through Sephacryl S400 spin columns (GE ...
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bioRxiv - Neuroscience 2021Quote: ... which were then blocked during 1h at RT with Superblock™ in PBS blocking buffer (Thermo fisher Scientific #37515) or PBS containing 0.1% Tween 20 and 3% BSA ...
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bioRxiv - Immunology 2023Quote: ... Slides were washed twice in PBS-T for 5min and incubated for 1h with anti-rabbit AlexaFluor555 (Invitrogen, #A32794) or anti-rabbit AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Electrophoresis was carried out in MOPS buffer by applying a constant current of 50mA for 1h (Electrophoresis kit, Invitrogen). Tubulin concentrations were assessed either by using Coomassie staining or acetylated tubulin content ...
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bioRxiv - Neuroscience 2022Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphate buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Physiology 2022Quote: ... Hearts were again washed trice in PBT (0.2% Triton X-100 in PBS) for 1h each and incubated in blocking solution (10% FBS (4133, Invitrogen), 1% BSA (A7030-10G ...
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bioRxiv - Neuroscience 2024Quote: ... eBiosciences) for 1h before sections were mounted in 0.1 M phosphat buffer and cover-slipped with Flouromount G (Invitrogen) mounting media ...
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bioRxiv - Evolutionary Biology 2023Quote: ... corresponding secondary antibodies were added for 1h at RT: (Alexa-594-conjugated goat anti-rabbit IgG (H+L) (Invitrogen), Alexa-594-conjugated goat anti-chicken IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... and incubated for 1h at RT with a chicken anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies, Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were mounted with Fluoromount G™ containing 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI, Invitrogen) for nuclei detection ...
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bioRxiv - Cell Biology 2021Quote: ... at a 1:1:1 ratio (5 mg each) using 250 ml of Opti-MEM (Gibco, 31985088) and 45 ml (1 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... or DRAQ5 (1:500; 5 min; RT; Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng of GeneRuler 1 kb DNA ladder (Thermo Scientific) was used as a standard for DNA length ...
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bioRxiv - Bioengineering 2021Quote: ... 1 x 5 mg (Thermo Fisher Scientific; cat. no: A44520). Note ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 5 g L−1 yeast extract (ThermoFisher Scientific) (Sorg and Dineen 2009) ...
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bioRxiv - Cell Biology 2022Quote: ... and fetal bovine serum (1:5 Thermo Fisher Scientific A3160801)) and cells were spun down for 6 min at 650 rcf ...
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bioRxiv - Cancer Biology 2022Quote: ... and HEPES (1 M, 5 ml, Gibco, cat no. 15630049) were added to a full bottle of advanced DMEM/F12 (500 ml ...
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bioRxiv - Cell Biology 2020Quote: ... 5% fetal bovine serum (FBS) and 1 % penicillin/streptomycin (Gibco, Life Technologies,
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bioRxiv - Immunology 2021Quote: ... containing 1 µl Glycogen (5 µg/µl, Thermo Fisher Scientific). 0.8 µg total RNA was used for reverse transcription and 0.5 µl of cDNA was used for quantitative real time polymerase chain reaction (qRT-PCR) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 5% FBS (Bodinco) and 1% Pen/Strep (Invitrogen) (Kortekaas et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 1% GlutaMax and 5 g/mL gentamycin (all from Gibco) at 37 °C in humidified atmosphere (95% air ...
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bioRxiv - Neuroscience 2024Quote: ... serotonin receptor 2C (5-HTR2C; Invitrogen, Waltham, MA; 1:1000). Membranes were washed three times in 0.1% Tween-20 in 1X TBS (TBST ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-SST (Thermo Fisher, PA-5-85759, 1:250), mouse anti-NeuN (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit IgG anti-claudin-5 (Invitrogen, #34-1600, 1:1000), and rabbit IgG anti-NLRP3 (Cell Signaling Technologies ...
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bioRxiv - Genomics 2024Quote: ... 1 μl of 5 mg/mL Glycogen was added (Invitrogen) per sample ...
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bioRxiv - Physiology 2024Quote: ... and 5 µL of 1-µm fluorescent beads (Invitrogen, #F13080) were intravenously injected prior to imaging ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 ml of 5 μM Rapamycin (Fisher Scientific BP2963-1) + 150 nM GSK-A1 (Sigma SML2453 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5) Opal 690/anti-CD34 (1:100, QBEND/10, Invitrogen), 6 ...
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bioRxiv - Cell Biology 2022Quote: ... 5□ng□ml−1 β-FGF (PHG0026, Thermo Fisher Scientific) and 1% P/S.
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bioRxiv - Cell Biology 2022Quote: ... 0.5 ml of 5 μM Rapamycin (Fisher Scientific BP2963-1) + 150 nM GSK-A1 (Sigma SML2453 ...
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bioRxiv - Cell Biology 2023Quote: ... and goat anti-rabbit Cyanine 5 (Invitrogen, A10523, 1:200) were applied for 2 hours at RT in the dark ...
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bioRxiv - Systems Biology 2023Quote: ... 5) Opal 690/anti-CD34 (1:100, QBEND/10, Invitrogen), 6 ...