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Citations for Thermo Fisher :
1451 - 1500 of 2644 citations for 8 Bromoguanosine hydrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The conditioned media was replaced by a transfection mixture composed of 8 μg DNA (BACE:CH and APP:VN and BACE1:VC) and 10 μl of Lipofectamine 2000 (ThermoFisher) in 1.0 ml of Optimem (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... Then the enzyme-inhibitor mixture was placed in a 6–8 kDa MWCO dialysis membrane (Fisher Scientific, Canada) and dialyzed against 2 L of 50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in Urea buffer (8 m Urea, 50 mm ammoniumbicarbonate, Halts Protease-phosphatase-inhibitor (Thermo Scientific)) ...
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bioRxiv - Neuroscience 2019Quote: ... A custom made titanium ring (10 mm diameter) containing an 8 mm glass coverslip (Thermo Fisher Scientific, UK) was inserted in the craniotomy and secured to the skull with dental cement ...
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bioRxiv - Neuroscience 2019Quote: ... The crosslinking reaction was stopped with 1/10 volume of 1.25 M glycine pH 8 (Thermo Fisher Scientific). Crosslinked cell pellets were washed 3 times with ice-cold DPBS followed by lysis or snap freezing and storage at −80°C ...
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bioRxiv - Biochemistry 2021Quote: ... and Halo (OD500 ~8) GVs sample was frozen using a Mark IV Vitrobot (FEI, now Thermo Fisher Scientific) (4°C ...
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bioRxiv - Microbiology 2021Quote: ... 8 x 106 HEK293T cells were plated in 10-cm tissue culture dishes and transfected using Lipofectamine2000 (Invitrogen) with plasmids encoding different CoV spike proteins or VSV-G protein ...
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bioRxiv - Microbiology 2020Quote: ... Cells in antibiotic free media (8×105/6-well) were transfected 6 hours post infection with 2μg plasmid and 6μl Lipofectamine 2000 (Invitrogen) per well diluted in Opti-MEM (Invitrogen).
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bioRxiv - Evolutionary Biology 2021Quote: ... at a 1:8 dilution with an ABI PRISM 3130 Avant Genetic Analyser automated sequencer (Applied Biosystems, USA) and the primers included in the PCR protocols.
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bioRxiv - Cancer Biology 2020Quote: ES cell-derived haemogenic endothelium cultured on gelatine-coated 8-well Lab-Tek II Chamber Slide System (ThermoFisher) was fixed using 4% PFA for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... were maintained at 37 °C in a humidified atmosphere of 8% CO2 and cultured in high-glucose Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... 8 × 106 cells were seeded in a 15-cm plate with 45 mL of DMEM for SILAC (ThermoFisher) supplemented with foetal calf serum (10% v/v) ...
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bioRxiv - Microbiology 2021Quote: ... were washed twice with 300 μL 1X TBS-T (2.4 g Tris-Base, 8 g NaCl, 0.1 % Tween20) using a magnetic stand (ThermoFisher) before coupling them with 200 μl of B domains in TBS at 0.125 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... the indicated cells were transfected with LentiCRISPR-V2 plasmid carrying sgARID1A (Supplementary Table 8) using Lipofectamine 2000 (Invitrogen), and further selected by 1 μg/mL puromycin (Selleck ...
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bioRxiv - Developmental Biology 2022Quote: ... minced and processed into a single cell suspension in a Collagenase I (8 mg/5ml, 17100017, Life Technologies) /Dispase (1:10 ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed using NuPAGE 4-12% Bis-Tris and 3-8% Tris-Acetate gels (Life Technologies). Nitrocellulose membranes were developed with horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2019Quote: ... the protein samples were separated on 4-20% SDS-PAGE or Bolt™ 8% Bis-Tris gels (Invitrogen) for 1 h and blotted onto PVDF membrane ...
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bioRxiv - Bioengineering 2020Quote: Day 8 blastocysts were labelled and imaged within devices using the ratiometric mitochondrial dye JC-10 (Molecular Probes). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of proteins were resolved on 8% Bis-Tris page gel (Invitrogen, Thermo Scientific, Waltham, MA, USA). Proteins were resolved eletro-trasferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... Equal amounts of proteins were resolved on 8% Bis-Tris page gel (Invitrogen, Thermo Scientific, Waltham, MA, USA). Proteins were resolved eletro-trasferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... The droplets were injected with a reverse transcription mix constituted of 25 mM Tris-HCl pH 8 (Invitrogen), 8 mM DTT (Invitrogen) ...
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bioRxiv - Bioengineering 2020Quote: ... or P[8] under control of the AOX1 promoter using a commercial vector (pPICZ A, Thermo Fisher Scientific). Modified vectors containing markers for selection on G418 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 × 107 HTR-8/SVneo cells were homogenized using RIP lysis buffer plus RNase inhibitor (Thermo Fisher Scientific) and a proteinase inhibitor cocktail (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... 8-30μg of whole-cell extract was separated on precast 4 to 12% bis tris protein gel (Invitrogen) and transferred to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2019Quote: ... Mixtures of 20-μl of the sample (8-10 μM final, considering hexamers) and SYPRO Orange (× 100; Invitrogen) in Sol C were subjected to a temperature gradient from 20 to 100 °C with increments of 0.3 °C every 10 sec ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were 1 x washed and incubated with 8 µg / ml Alexa488 labelled anti Mouse IgG (ThermoFisher, A11001) for 1 hr at RT in the dark ...
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bioRxiv - Genomics 2021Quote: ... cells were resuspended in 1 mL cold lysis buffer: 10 mM Tris-HCl pH 8 (Thermo Scientific, AM9855G), 10 mM NaCl (Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... hiPSC-IMR90-1 cells were cultured on a Matrigel-coated 6-well plate with Essential 8 medium (Gibco). The medium was changed daily ...
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bioRxiv - Cancer Biology 2020Quote: ... was coated with Oregon Green 488-conjugated gelatin (Invitrogen; diluted 1:8 in solution of 2.5% (w/w) gelatin/2.5% (w/w ...
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bioRxiv - Neuroscience 2021Quote: ... 200 µg of human serum) were separated by SDS-PAGE on 3-8% tris-acetate gels (NuPAGE, Invitrogen) under reducing conditions at 150 V (cellular fraction ...
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bioRxiv - Molecular Biology 2021Quote: ... was added into the 48S IC and 80S IC mixtures in reverse-transcription buffer (0.5 mM dNTPs, 8 mM MgCl2, 0.15 U/μL SuperScript III reverse transcriptase (Invitrogen)) and the reaction was incubated for 45 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... for 2.5 s with a blot force of 8 using a Vitrobot Mark IV (Thermo Fisher, Waltham, MA) set to 4°C with 100% humidity ...
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bioRxiv - Cancer Biology 2020Quote: 3D acini were set up as described above in either 8-well coverglass chamber slides (Nunc, LabTek-II) or in 96 well plates (Greiner ...
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bioRxiv - Cell Biology 2020Quote: ... 1.8 mM CaCl2, 6 mM NaHCO3, 5.5 mM D-glucose, 25 mM HEPES, pH 7.4 supplemented with Gibco MEM Amino Acids and MEM Non-Essential Amino Acids solution ...
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bioRxiv - Genetics 2021Quote: ... PCR products were directly run on an 8% PAGE gel and visualized with SYBR Gold (ThermoFisher S-11494), or subject to AMPure XP beads (Beckman Coulter A63881 ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... samples were loaded under non-reduced conditions on a precasted 3-8% Tris-Acetate SDS-PAGE gel (Invitrogen). Proteins were transferred to a nitrocellulose membrane using an iBlot2 dry blotting system (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells at 5000 or 10000/cm2 were cultured on an 8-well chamber slide (Thermo Scientific, cat# 177445) or in a 6-well plate coated with 1 mg/ml Matrigel (Corning ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Genetics 2019Quote: ... A dye stock was prepared containing 0.3 mM Fluo-8 AM and 3.3% Pluronic F-127 (ThermoFisher Scientific) and mixed thoroughly ...
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bioRxiv - Genetics 2020Quote: Up to 10μg of total protein was resolved on NuPAGE™ 3-8% Tris-Acetate Protein Gels (Invitrogen) in 1x NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... and then analysed on Novex WedgeWell 4-12 % Tris-Glycine Gels or 3-8 % Tris-Acetate gels (Invitrogen). Tris-Glycine gels were run at 180 V for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... and HCT116 cells were transfected with 8 µg each of appropriate constructs using Lipofectamine 2000 (Thermo Fisher Scientific). Approximately 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged again at 500g for 8 min and resuspended in PBS (Gibco, cat. no. 14190-094) with 0.04% BSA (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions (200 μL) were incubated for 4 h in 8-well Nunc Lab-Tek chambers (Thermo Scientific #155361). Brightfield and Cy3 fluorescence images were captured on a Nikon Ti Eclipse wide-field microscope ...
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bioRxiv - Genetics 2022Quote: ... On the day of transfection 8 μg of plasmid DNA was diluted in 125 μl Opti-MEM (Thermofisher) and 25 μl of FuGENE Reagent was diluted with 100 μl Opti-MEM ...