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Citations for Thermo Fisher :
1551 - 1600 of 8052 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and then primers listed in Supplemental Table 2 on a ViiA 7 real-time PCR system (Applied Biosystems) with the following cycling parameters ...
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bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed twice and resuspended in FACS buffer containing 7-AAD viability staining solution (Life Technologies). Cell sorting was performed using a BD FACSAria III (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Cells were differentiated for 7 days in RPMI 1640 medium with L-glutamine (Gibco Thermo Fisher Scientific 21875034) supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001 ...
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bioRxiv - Neuroscience 2023Quote: ... equal amounts of protein were loaded in 7% Bis-Tris gels and run with MOPS buffer (Thermo Scientific) and transferred to membranes (Amersham GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... washed with 1 x PBS (pH 7) and subsequently labelled with 25 μg/ml AF488 C5 Maleimide (Invitrogen) for 10 min in the dark followed by at least two additional washing steps ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR for pri-let-7 and eft-2 were performed with Absolute Blue SYBR Green (Thermo Scientific) on the CFX63 Real Time System Thermocyclers (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... The qRT–PCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System (Thermo Fisher Scientific). The sequences of primers and probes for Adar1 p150 and GAPDH have been previously reported (16) ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Real-time quantitative RT-PCR (Q-PCR) was performed in a QuantStudio 7/12k Flex System (Applied Biosystems) with the following primers Crbn.F 5’-TGAAATGGAAGTTGAAGACCAAGATAG-3 ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggregation medium (BSA supplemented N2B@7 media) consisted in 500ml 1:1 mix of Neurobasal (Invitrogen 21103-049) and DMEM/F12 (Invitrogen 21331) ...
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bioRxiv - Neuroscience 2023Quote: ... 7×106 cells were plated in 12ml of transfection media (Opti-MEM I Reduced Serum Medium (Gibco #31985070), 1x GlutaMAX Supplement ...
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bioRxiv - Biophysics 2023Quote: ... potassium phosphate buffer (KP) 0.5 M at pH = 7 (adjusted with pH meter Orion Star A111, Thermo Fisher). BSA and urease stock solutions concentration are measured after their preparation using a UV-vis spectrophotometer (Cary 60 Spectrophotometer ...
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bioRxiv - Cell Biology 2023Quote: ... MCF-7 cells (HTB-22) and A431 cells (CRL-1555) were all cultured in DMEM (Gibco, 31966-021) supplemented with 10% FCS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
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bioRxiv - Biochemistry 2023Quote: ... This solution was incubated for 1h at ~7°C with 0.25 ml of HisPur Cobalt resin (Thermo Scientific). The beads were allowed to settle to the bottom of a protein purification column and washed 5 times with 10 ml of washing buffer comprising 10 mM imidazole and 0.1% Triton X-100 in 2xPBS ...
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bioRxiv - Biophysics 2023Quote: ... The DTT was removed using a 2 ml ZebaSpin MWCO 7 kD desalting column (Thermo Fisher Scientific, USA). The columns were pre-equilibrated by washing them with 1 ml 10 mM TRIS buffer at pH 7.5 for three consecutive times by spinning them at 1000 G for 2 minutes for each washing step ...
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bioRxiv - Cell Biology 2022Quote: Live/Dead™ Cell Imaging Kit and CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) were used for detection of cell apoptosis based on the manufacturer instructions ...
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bioRxiv - Biophysics 2023Quote: ... The protein was then buffer-exchanged into HBS using a 7 kDa Zeba desalting column (Thermo Fisher, 89882) and stored at -80 ºC until use.
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bioRxiv - Genomics 2022Quote: ... HPC-7 cells were maintained at density of 2-10 x105/ml in Iscove’s modified Dulbecco’s medium (Invitrogen) supplemented with 50 ng/ml of mouse stem cell factor (Gemini Bio-Products) ...
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bioRxiv - Biochemistry 2023Quote: ... Fluorescence data for SYPRO Orange signal was collected with a ViiA 7 Real-Time PCR System (Applied Biosystems) and QuantStudio Real-Time PCR Software v1.2 using 470 ± 15 nm excitation and 586 ± 10 nm emission filter ...
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bioRxiv - Biochemistry 2023Quote: ... for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific, Waltham, MA) in water ...
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bioRxiv - Cell Biology 2023Quote: ... HSPCs were incubated with 20µM NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen) for 30 minutes at 4°C for Golgi staining or Cytopainter (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments ...
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bioRxiv - Microbiology 2023Quote: ... using a QuantStudio 7 Flex Real-Time PCR System utilizing Fast SYBR™ Green Master Mix (Applied Biosystems).
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bioRxiv - Bioengineering 2023Quote: ... HUVECs were cultured for 6-7 days prior to detachment with 0.25% trypsin-EDTA (Life Technologies, 25200-056) for use in experiments.
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bioRxiv - Developmental Biology 2023Quote: ... HPC-7 cells were maintained at density of 1-10 ×105/ml in Iscove’s modified Dulbecco’s medium (Invitrogen) supplemented with 50-100 ng/ml of mouse stem cell factor (Gemini Bio-Products) ...
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bioRxiv - Immunology 2023Quote: ... All samples were performed in duplicates and measured on either ViiATM 7 Real-Time PCR System (ThermoFisher Scientific) or ABI Prism 7300 Sequence Detector (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
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bioRxiv - Molecular Biology 2023Quote: ... transfections were performed by adding the following to each well: 7 µL of pre-warmed OptiMEM media (Invitrogen), 500 ng of 5′ -capped and 3′ -polyadenylated Nluc reporter mRNA ...
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bioRxiv - Cell Biology 2023Quote: ... the IR-specific 83-7 antibody was coupled to Dynabeads™ MyOne™ Tosylactivated magnetic beads (Invitrogen™) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... HeLa and COS-7 cells were seeded onto dishes coated with 5 mg fibronectin (Life Technologies 33016-015) in diH2O ...
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bioRxiv - Cell Biology 2024Quote: ... Z-stack images were taking on day 6-7 after seeding using EVOS™ 7000 Imaging System (Invitrogen). and used for quantification of organoids.
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bioRxiv - Neuroscience 2024Quote: ... 1.5 μL of cDNA from each sample was run in duplicate using a QuantStudio 7 Flex (Thermo Fisher) and analyzed with the 2-ΔΔCT method.
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bioRxiv - Plant Biology 2024Quote: ... Data was analysed with QuantStudio 6 and 7 Pro Real-Time PCR Systems Software (Thermo Fisher Scientific, America). For RT-qPCR ...
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bioRxiv - Cell Biology 2024Quote: ... Treated and untreated (PBS) organoids were collected at day 7 and dissociated to single cells using TrypLE (Invitrogen) incubation at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Transcripts were quantified by real-time quantitative PCR on a Quantstudio 7 Real-Time PCR instrument (Thermo Fisher). Probes for 24 cryptic splice variants (and the respective canonical variants ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Cell Biology 2024Quote: COS-7 cells (ATCC CRL-1651) were cultured in DMEM (5.56 mM glucose, glutaMAX supplement, Thermo Fisher 10567022) supplemented with 10% fetal bovine serum (ThermoFisher 10438-034) ...
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bioRxiv - Microbiology 2020Quote: ... Tissues were fixed in formalin for 7 days then were transferred to 70% ethanol (ThermoFisher Scientific, Waltham, MA, USA) prior to trimming for embedding ...
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bioRxiv - Biophysics 2021Quote: Standard DNA-PAINT reagents were ordered and prepared according to [7]: buffer A (10 mM Tris-HCl pH 7.5, 100 mM NaCl; Ambion), buffer B (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was isolated from 7×106 cells using trizol according to the manufacturer’s instructions (Invitrogen - catalogue no. 15596026). Following precipitation ...
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bioRxiv - Cancer Biology 2021Quote: We performed apoptosis assays using the annexin-V FITC/PI kit manual (catalog# P50-929-7; Thermo Fisher Scientific). Cells treated with chemotherapeutics were resuspended in fresh medium and plated in a T-75 flask at 37 °C for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... then loaded with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethylester (BCECF-AM, 1.6 μM, Life Technologies) for 30 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Immunology 2021Quote: ... 2020) and reactions were carried out using a QuantStudio 6 and 7 Flex Real-Time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Ovaries collected from 7-day-old pups were cultured on polycarbonate membrane (Whatman Nucleopore Polycarbonate membrane) in MEM (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... and the hematopoetic stem cells were allowed to differentiate for 7 days in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...