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Citations for Thermo Fisher :
1751 - 1800 of 8052 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... total bone marrow cells were isolated from the femur and tibias of mice and differentiated into macrophages for 7 days in complete DMEM medium (500 mL, ThermoFisher) supplemented with M-CSF (40 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from pygidia and several posterior segments of young regenerates (7-10 days of regeneration) of Alitta virens and Platynereis dumerilii using TRIzol reagent (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng template was used per reaction with SYBR Green Mastermix in ViiA 7 Real-Time PCR System (Applied Biosystems). As endogenous control ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was then performed for at least 3 biological replicates using Biozym Blue S’Green qPCR Mix Separate ROX (No. 331416XL) on QuantStudio 7 Flex Real-Time PCR System from Applied Biosystems. Pax3 fold change was calculated from ΔCt using Gapdh as housekeeping gene (2^-(ΔCt)) ...
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bioRxiv - Developmental Biology 2021Quote: Micropatterned coverslips with 225-μm diameter synthetic lung buds at day 7 of lung induction were dissociated with TrypLE Express (Gibco) for 10 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... was cultured in Dulbecco’ ss Modified Eagle Medium (DMEM) supplemented with 7% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco) at 37°C with 5% CO2.
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bioRxiv - Immunology 2022Quote: ... was used and results were analyzed by quantitative RT-PCR with a Vii 7 Real-time PCR system (Applied Biosystems). The comparative threshold cycle method and internal control (Gapdh ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Developmental Biology 2020Quote: ... We use the following conditions for the 40-cycle reaction on the Quant Studio TM 7 Flex System (Thermo Fisher): a ...
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bioRxiv - Microbiology 2019Quote: ... Samples with RIN > 7 and DNA contamination <10% were used for cDNA synthesis using SuperScript III first-strand synthesis kit (Invitrogen). RT-qPCR was performed using 2xSYBR FAST qPCR universal MasterMix kit (Kappa Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... After 7-10d culture cells were shaken in an orbital shaker at 350-400rpm (1.9cm orbital radius, MaxQ 4450, ThermoFisher Scientific) at 37°C to obtain a culture of cortical astrocytes ...
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bioRxiv - Biophysics 2019Quote: 20-nm fluorescent dark red beads samples (Fig. 2d, Supplementary Fig. 5) were prepared using a 5.10−7 dilution of the initial solution (F8783, Thermo Fisher). We performed the dilution in PBS + 5% glucose to match the index of the dSTORM imaging buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF-7 and MDA-MB-231 cells were plated on glass coverslips in serum-free Gibco OptiMEM medium (Life Technologies) and transduced with 4.8×1010 PFU of Ad5-Laconic (Vector Biolabs) ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... of WT and Adrb2-/- Cl4 mice (7-12 weeks old) with a negative isolation kit according to the manufacturer’s instructions (Invitrogen #11417D). Donor T cells were derived from CD90.1 congenic animals in order to track their frequency in CD90.2 hosts ...
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bioRxiv - Neuroscience 2020Quote: ... Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at −80 °C until sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Biochemistry 2021Quote: ... cell pellets were incubated with 300 µL of 10 mg/mL lysozyme (prepared fresh in sterile H2O) and incubated for 7 minutes at 30°C/250 rpm to allow for efficient lysis by TRIzol reagent (Ambion). 10-15 glass beads (4mm diameter ...
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bioRxiv - Bioengineering 2021Quote: ... All proteins were purified with desalting columns (Zeba Spin Desalting Columns, 0.5 mL with 7 kDa MWCO, Thermo Fisher Scientific) by washing with PBS three times (centrifuging 1500 rcf ...
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bioRxiv - Bioengineering 2020Quote: ... A biphasic solvent overlay was employed to capture patchoulol by adding 7 mL of filter-sterilized n-dodecane (Acros Organics) to each culture (10% v/v) ...
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bioRxiv - Bioengineering 2020Quote: ... The samples were sectioned using Kawamoto’s cryofilm type 2C (SECTION-LAB Co. Ltd., Japan) to 7 µm thickness using a cryotome (CryoStar NX70, Thermo Scientific) according to the Kawamoto protocol [48] ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from HTM cells ± DEX at 7 d (N = 3 per group and donor) using PureLink RNA Mini Kit (Invitrogen). RNA concentration was determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Biochemistry 2020Quote: MCF-7 cells (150–1000 cells/well) were plated in Labtek Permanox Plastic Chamber slide system (Nunc, Thermo; cat. #177445) and incubated overnight ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Fractions containing purified protein were pooled and dialyzed overnight against storage buffer (0.02 M sodium phosphate, pH 7) in a 10 kD cut off dialysis cassette (ThermoFisher, 66380). Purified protein was stored at 4 °C for use within days or at −20 °C for longer-term storage.
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bioRxiv - Cancer Biology 2020Quote: ... and the doxycycline-inducible COV434 pIND20 BRG1-2.7 and TOV21G pIND20 ARID1A (generously donated by Dr. Bernard Weissman, University of North Carolina) were cultured in DMEM (Gibco) and supplemented with 10% TET free FBS (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... Eyes were first incubated in 1 mL of high glucose Hanks balanced salt solution/0.5 mg/ml proteinase K (37°C, 7 min) and then in Neurobasal medium (Life Technologies) plus 10% fetal calf serum to stop enzymatic activity ...
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bioRxiv - Plant Biology 2021Quote: ... pistils and young seeds (7 DAP from CT and 5 DAP from HT) were isolated following a protocol for Trizol (Invitrogen). RNA from 26 DAP seeds was isolated using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2020Quote: ... qPCRs were carried out on an Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Applied Biosystems, Foster City, California, USA) using isolated genomic DNA as template ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cultures were grown at 37°C either in M9 media [M9 salts (248510, Difco)] supplemented with 0.4% glucose (6363-53-7, Fisher Scientific), 1 mM MgSO4 (Sigma Aldrich) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Microbiology 2021Quote: ... and qPCR were performed on 5µL of the cDNA libraries diluted at 1:10 using the Precision Plus 2x qPCR Mastermix (Primer Design) according to the manufacturer’s instructions and the Quant Studio 7 Flex (Applied Biosystems). For validation of the translatomic results ...
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bioRxiv - Cell Biology 2020Quote: Cos-7 cells were maintained in DMEM + 10% fetal bovine serum and all transfections were conducted using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... HEK 293T/17 (ATCC® CRL-11268) and LLCMK-2 (monkey kidney epithelial cells, ATTC CCL-7) were maintained in Tissue Culture Medium (DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR was performed using 384-well plates (4titude Ltd, UK) in the ViiA™ 7 System (Applied Biosystems, UK) with the SensiFAST™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... Strip tubes were loaded onto the Genie-II or Genie-III (OptiGene Ltd) and 96-well plates run on a QuantStudio 7 thermocycler (ThermoFisher). Reactions were incubated at 65°C for 30 min with fluorescence acquisition every 30 sec (Genie instruments ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions with RNase T2 were done at room temperature for 10 minutes in 1× Structure Buffer (10 mM Tris pH 7, 100 mM KCl and 10 mM MgCl2; Ambion). Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 7 x 107 MRC5 cells were infected as described above and total RNA was isolated from cells using TRIzol (Invitrogen), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell death was assessed by measuring caspase 3 cleavage using a CellEvent Caspase3/7 Green Flow Cytometry kit (Thermo Fisher), according to the manufacturer’s descriptions ...