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Citations for Thermo Fisher :
1801 - 1850 of 8052 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... was mixed with Taqman assays and analysed in a 384 well QuantStudio Flex 7 (Applied Biosystems Life Tech, Thermo Scientific) in 5μl reaction volumes ...
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bioRxiv - Cancer Biology 2020Quote: ... was mixed with Taqman assays and analysed in a 384 well QuantStudio Flex 7 (Applied Biosystems Life Tech, Thermo Scientific) in 5μl reaction volumes ...
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bioRxiv - Cell Biology 2021Quote: ... The quantitative PCR (Q-PCR) reactions were conducted in triplicate on a ViiA 7 Real-Time PCR system (Thermo Fisher) from 1 μL of cDNA using the PerfeCTa SYBR Green SuperMix reagent (Quantabio Cat# ...
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bioRxiv - Cell Biology 2021Quote: ... The tissues were then digested in room temperature for 7 min in a 3-mL Hank’s solution containing 0.25% Trypsin (Thermo Fisher, 15090046) and 0.1 μg/μL DNase (Sigma D5025 ...
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bioRxiv - Cancer Biology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher Scientific, 89960; IL). The original murine anti-PD1 monoclonal antibody (63 ...
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bioRxiv - Genetics 2022Quote: ... The volume was filled up to 7 ml with water and 50 units of T4 DNA ligase (Thermo Fisher Scientific) were added ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... and qPCR was performed on 5μL of the cDNA libraries diluted at 1:5 using the Precision Plus 2x 598 qPCR Mastermix (Primer Design) according to the manufacturer’s instructions and the Quant Studio 7 Flex (Applied Biosystems).
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bioRxiv - Immunology 2022Quote: pDC generation: bone marrow cells were isolated (see above) and cultured for 7 days in RPMI-1640 GlutaMAX-I (GIBCO) supplemented with 10% FCS (GIBCO) ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 350ng of pCAGIG-VENUS was used to transfect the neurons two days after adding mouse glia (PNI day 7) using Lipofectamine 2000 (ThermoFisher). A full media change using conditioned media was performed 5-6 hours post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... to final concentration 0.5 mg/ml protein and 6x dye and heated from 10 to 70 °C with fluorescence reading every 0.1 °C (4 to 5 sec) in a QuantStudioTM 7 Flex Real-Time PCR System (Life Technologies). Protein thermal melting curve were generated using the Protein Thermal Shift TM software ...
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bioRxiv - Neuroscience 2022Quote: ... jGCaMP7b-XC or jGCaMP7b-XN and 1 μg cDNA encoding CFP (for labelling the soma area and neurites) were transiently transfected into DIV 5-7 cultured cortical neurons by Lipofectamine 2000 (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Microbiology 2022Quote: ... as per manufacturer’s instructions in 96-well plates and conducted in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Primers were designed using Primer3 94 to target the hucL gene (HucL_fw ...
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The diluted sample was then passed through a Zeba Spin Desalting Column (7 kDa cut-off, Thermo Fisher Scientific, France) equilibrated in acquisition buffer containing 0.005% LMNG (w/v) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted from rats aged postnatal day 5-7 and placed in working buffer (Hank’s balanced salt solution (HBSS) (Life Technologies) with 0.5% chromatographically purified bovine serum albumin (BSA ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
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bioRxiv - Microbiology 2021Quote: ... The number of PCR cycles for sufficient DNA for downstream applications was empirically determined by quantitative PCR (ViiA 7 Real-Time PCR System, ThermoFisher) in the presence of 1X Evagreen (20X Evagreen ...
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bioRxiv - Cancer Biology 2020Quote: ... Reactions were performed using the pre-set program of the ABI ViiA 7 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... marrow was extracted from femurs and tibias of 8 to 12 week-old male and female mice and differentiated for 7 days into BMM in Dulbecco’s Modified Eagle’s Medium with glutamine (DMEM, Thermo Fisher Scientific), containing 10% heat-inactivated ...
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bioRxiv - Microbiology 2022Quote: ... targeting a region in the viral ORF1ab gene and using a QuantStudio™ 7 Real-Time PCR System (Applied Biosystems). Data were processed using GraphPad Prism software (V8.0 ...
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bioRxiv - Genetics 2022Quote: ... were combined and concentrated to ∼1 mL and dialyzed using 7 KMWCO Snake Skin dialysis tubing (Thermo Scientific, Waltham, Massachusetts) into 20 mM Tris 150 mM NaCl pH 8.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Real-time quantitative PCR was performed on a ViiA 7 Real-Time PCR System with OptiFlex Optics System (Applied Biosystems) using PowerUp SYBR Green PCR kit (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative PCR was performed using SYBR green reagents on an Applied Biosystems QuantStudio 3 and ViiA 7 Real-Time PCR Systems (ThermoFisher).
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bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
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bioRxiv - Molecular Biology 2019Quote: ... 250 ng of m1A-containing synthetic oligonucleotide (see Extended Data Table 7) were spotted in triplicate on a BrightStar membrane (ThermoFisher), allowed to briefly air-dry ...
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bioRxiv - Biophysics 2019Quote: ... N-((2-(iodoacetoxy)ethyl)-N-Methyl)- amino-7-Nitrobenz-2-Oxa-1,3-Diazole (IANBD ester) and Oregon Green 488 maleimide were purchased from LIFE TECHNOLOGIES LTD (Paisley ...
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bioRxiv - Biophysics 2019Quote: ... N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine] (Thermo Fisher Scientific, Inc., Waltham, MA). Y-ATRAM (Table 1 ...
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bioRxiv - Pathology 2020Quote: ... Gene expression by qPCR was performed using a ViiA™ 7 Real-Time PCR System (Applied Biosystems, Carlsbad, CA, USA). All reactions were performed as biological triplicates and technical duplicates using a GoTaq® qPCR Master Mix Kit (Promega) ...
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bioRxiv - Biochemistry 2019Quote: ... RNAs after hDcp2 treatment were resolved electrophoretically on denaturing 15% acrylamide / 7 M urea 1xTBE gel stained with SYBR Gold (Invitrogen) and visualized using a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Microbiology 2019Quote: ... LC-MS/MS data was collected as previously described.7 The raw data files were processed and quantified using Proteome Discoverer software v1.4 (Thermo Scientific) and searched against bacterial genome and horizontally acquired resistance genes as described previously.8
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bioRxiv - Systems Biology 2019Quote: All the reactions were performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems by Life Technologies). Cycling conditions were ...
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bioRxiv - Systems Biology 2019Quote: All the reactions were performed on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems by Life Technologies). Cycling conditions were ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed on an ABI PRISM 7900HT or QuantStudio 7 Flex Real-Time PCR System (Life Technologies) with Power SYBR Green PCR Mix (Life Technologies) ...
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bioRxiv - Physiology 2019Quote: ... Quantitative Real-time PCR (qRT-PCR) was performed in duplicate using the ViiA 7 Real-time PCR system (Applied biosystems). All expression data were normalized by the 2(-ΔCt ...
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bioRxiv - Microbiology 2020Quote: ... The dose-response plates are 200 times diluted in 300μL Phosphate Buffered Saline (PBS; pH= 7-7.2 without Ca/Mg) (Gibco, 14190-144) by transferring 1.5 μL of serially diluted compound ...
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bioRxiv - Physiology 2020Quote: ... and incubated in cell* dishes at 37° C for 4 – 7 hours before adding cell culture medium (RPMI 1640, -glucose +glutamine, Gibco), supplemented with penstrep (10,000 U/ml penicillin / 10,000 μg/ml streptomycin ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Quantitative transcript analysis was performed using a QuantStudio 7 Flex Real-Time PCR System with the Power SYBR Green RNA-to-Ct Real-Time qPCR Kit (all ThermoFisher). Total RNA template (25 ng per reaction ...
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bioRxiv - Pathology 2021Quote: ... Quantitative miR expression data were acquired and analyzed using the ViiA 7 real-time PCR system (Applied Biosystems, Cat# 4453545).
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bioRxiv - Pathology 2021Quote: ... and 001234) and ViiA 7 real-time PCR system (Cat# 4453545) were purchased from Applied Biosystems (Foster City, CA, USA). Rabbit polyclonal anti-calnexin (CNX ...
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bioRxiv - Molecular Biology 2021Quote: ... using a 7900HT Fast Real-Time PCR System or a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). The mRNA expression levels were calculated with the 2-ΔΔCt method ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time PCR was performed and analysed using the ABI ViiA™ 7 real time PCR system (Thermo Fisher Scientific). TaqMan Gene Expression Assays (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2020Quote: ... African Green monkey kidney epithelial cells) and Huh-7 cells (human hepatocellular carcinoma cells) were cultured in Dulbecco’s minimal essential medium (DMEM; Life Technologies), supplemented with 10 % fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... Digestion was stopped by addition of an 8.6% SDS/7 mM EDTA solution and crosslinks were reversed by adding Proteinase K (20 mg/ml; Life Technologies) and incubating 6 hr at 65°C ...
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bioRxiv - Cell Biology 2019Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (AL007A, HiMedia, Mumbai, Maharashtra, India) supplemented with 10% FBS (10270-106, GIBCO-Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and from day 7 cells were fed with fresh RPMI medium supplemented with B27 with insulin (#17504044, Thermo Fisher Scientific) without extra supplement ...