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Citations for Thermo Fisher :
1451 - 1500 of 8052 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and 10% glycerol) using 0.5-mL Zeba™ spin desalting column (7-kDa MWCO) (ThermoFisher Scientific, #89882). The solution was warmed to room temperature and 5 μL of 5 mg/mL taxol-stabilized MTs was added ...
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bioRxiv - Immunology 2024Quote: ... and qPCR experiments were completed on a QuantStudio 7 flex real-time PCR system (Applied Biosystems, 4485701) using the Taqman gene expression master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... LLC-MK-2 cells (rhesus monkey kidney; CCL-7/ATCC) were cultured in MEM (GIBCO #11095-080) supplemented with 100 units/ml penicillin and 100 μg/ml streptomycin (P/S) ...
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bioRxiv - Neuroscience 2023Quote: The brains of 7-day-old flies were dissected in Schneider’s insect medium (Thermo Fisher Scientific, 21720). They were then fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... Non-Tg and PS19 neurons were transfected with various constructs at DIV5-7 using Lipofectamine 2000 (Invitrogen) followed by time-lapse imaging 10-14 days after transfection prior to quantification analysis.
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Bioengineering 2024Quote: ... and passaged every 7 days by treatment with TrypLE Select Enzyme (Thermo Fisher Scientific Inc., MA, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were run and data analyzed using the QuantStudioTM 7 Flex Real-time PCR system (Applied Biosystems). Data were calculated using the 2-△△Ct method.
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bioRxiv - Bioengineering 2024Quote: ... Herceptin was buffer exchanged in PBS by using 7 K MWCO Zeba Spin Desalting Columns (ThermoFisher Scientific). Then it was incubated with 20ME (Molar equivalent ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher) at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred to nitrocellulose membranes using the iBlot 7-min blotting system (Thermo Fisher Scientific). Membranes were blocked for 1 h at room temperature in 1 x casein blocking buffer (Sigma ...
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bioRxiv - Immunology 2019Quote: ... and Para-nitro-phenyl-phosphatase (PNPP) (Thermo Fisher Scientific, USA) substrate ...
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bioRxiv - Microbiology 2020Quote: ... upon which 1 μg of yeast RNA and 10× structure buffer (0.1 M Tris at pH 7, 1 M KCl, 0.1 M MgCl2; Ambion) were added ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... cultured neurons were transfected at 7 days in vitro (DIV) with a commercial calcium phosphate transfection kit (Invitrogen) as previously described49 ...
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bioRxiv - Cancer Biology 2021Quote: ... and qPCR was performed using let-7 and 2S rRNA TaqMan MicroRNA Assay primer mix (Thermo Fisher Scientific) with the TaqMan Small RNA Assay (Applied Biosystems).
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Cell Biology 2020Quote: Following buffer exchange to 100 mM NaHCO3 with 0.5 ml 7 kDa Spin Desalting Columns (Thermo Fisher, 89882), the anti-TFAM (TA332462 ...
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bioRxiv - Cell Biology 2020Quote: ... 7- Amino-4-Chlormethylcumarin (CMAC) was part of the Yeast Vacuole Marker Sampler Kit (Y7531) from Thermo Fisher and staining was performed according to manufacturer recommendations ...
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bioRxiv - Physiology 2019Quote: ... Malpighian tubules from 7-day-old adult female flies were dissected under Schneider’s insect medium (Thermo Fisher Scientific) and isolated into 10 μl drops of a 1:1 mixture of Schneider’s medium ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) was purchased from Invitrogen. Calcein dye ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Cell Biology 2019Quote: ... q-PCR was performed using a ViiA 7™ Real Time PCR System (Applied Biosystems, Life Technologies, UK) programed to perform the following steps ...
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bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... roots of 7-day-old seedlings were incubated with 50 µM Amplex™ UltraRed Reagent (AUR, Molecular Probes) for 20 min in dark conditions and rinsed with liquid MS ...
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bioRxiv - Microbiology 2021Quote: ... mRNA expression of the indicated genes was analyzed by QuantStudio 7 Flex Real Time PCR System (Applied Biosystems) using SYBR green master mix ...
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bioRxiv - Microbiology 2020Quote: ... Vero E6 and Huh-7 cells (Apath LLC, Brooklyn) were maintained in Dulbecco’s modified eagle medium (DMEM, Gibco) supplemented with 10 % heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: EV-rich fractions (7-10) from SEC were initially determined by protein (Pierce™ BCA Protein Assay, ThermoFisher) and particle quantification using nanoparticle tracking analysis (NTA ...
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bioRxiv - Bioengineering 2021Quote: ... Apoptosis was detected by incubating devices with 5μM of CellEvent™Caspase 3/7 Green detection reagent (Invitrogen) for 30 minutes at 37°C.
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bioRxiv - Biochemistry 2020Quote: ... Hs00961560_m1 Hs INSR) and the QuantStudio™ 7 Flex Real-Time PCR system (Thermo Fisher Scientific, MA, USA) and results analysed by the comparative Ct method ...
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bioRxiv - Cancer Biology 2020Quote: ... were used to analyse mRNA expression levels using a QuantStudio 7 Flex RT PCR machine (Thermo Fisher Scientific). TaqMan probes used were FAP (Hs00990791_m1) ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a total volume of 10 μl on a ViiA 7 system (Thermo Fisher Scientific). Gapdh was used as a housekeeping gene and data were evaluated by conversion to ΔCt values.
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bioRxiv - Cell Biology 2021Quote: ... Both COS-7 and HEK 293T were transfected by means of Lipofectamine and PLUS reagent (Thermo Fisher Scientific). The calcium phosphate transfection method was instead used to transfect WT or ΔOLF Lphn2-HA-pCCL in HEK 293T cells and to produce the corresponding viral particles subsequently employed to transduce ECs ...
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bioRxiv - Microbiology 2021Quote: ... a 20μL reaction was loaded into a ViiA 7 Real Time PCR system (Thermo Fisher Scientific, Waltham, MA). The following conditions were used ...
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bioRxiv - Molecular Biology 2021Quote: ... quantitative RT-PCR analysis was performed using a sequence detection system (ABI ViiA 7 system; Thermal Fisher Scientific). The following primers sets were used for analysis ...
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bioRxiv - Neuroscience 2020Quote: ... 2x SYBR master mix (Thermoscientific) were used and the reaction was run in Thermocycler (Applied Biosystems ViiA 7) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... MEL cell line-derived clones 4D7 and 2M12 (7) were maintained in 20 µg/mL of blasticidin (Invitrogen) and 1 mg/mL of G418 (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...
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bioRxiv - Biophysics 2020Quote: Microinjection was performed in COS-7 cell line cultured in 10% (v/v) fetal bovine serum (Life technologies) DMEM at 1·105 cell/well density ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... were used for experiments in passage 4 to 7 and were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of 7-amino-actinomycin D (7AAD, 50 μg/ml working solution in PBS, Thermo Fisher Scientific) was added to the stained cells 5–10 min before measurement.
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were incubated for 30 min in standard media with CellEvent Caspase 3/7 (1:400, C10423, Invitrogen) prior to imaging to observe apoptosis.
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bioRxiv - Bioengineering 2022Quote: ... COS-7 cells were plated onto Nunc Lab-Tek II Chambered Coverglass (Thermo Fisher Scientific, 12-565-335) to achieve ~70% confluence before transfection.
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bioRxiv - Neuroscience 2022Quote: ... Tax ID 10090) protein database (2017-6-7) using Proteome Discoverer (PD) 2.2 (Version 2.2.0.388; Thermo Fisher Scientific). Label-free quantification was also performed with PD 2.2 using precursor ions quantifier nodes ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured for 5-7 days at P0 before been passaged with Tryspin - EDTA 0.05% (Thermo Scientific, 25300054). Cells were passaged every 4-5 days and until p8 ...