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Citations for Thermo Fisher :
1501 - 1550 of 8052 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR assays were performed in technical quadruplicates in 384-well plates on QuantStudio 7 instrument (Life Technologies), with RT2 SYBR Green (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... prehybridization was carried out for 2 h at 42°C in 7 mL of prehybridization buffer ULTRAHyb (Ambion). Hybridization was performed overnight at 42°C in the same buffer in the presence of a [γ-32P]-labeled DNA oligonucleotide probe ...
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bioRxiv - Microbiology 2021Quote: ... Unbound GTP was removed using 2 mL Zeba column with 7 kDa molecular weight cutoff (Thermo Scientific, 89889) and exchanged into 30 mM Tris pH = 7.5 buffer containing 1 mM DTT ...
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bioRxiv - Molecular Biology 2022Quote: ... Quantitative Polymerase Chain Reaction (qPCR) was performed in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) using SYBR Green Real-Time PCR Master mix (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... Purification was performed using spin columns (7 kDa exclusion limit, 0.5 mL Zeba spin desalting columns, ThermoFisher Scientific). After purification ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transiently transfected into HEK293T or MCF-7 cells at 70% confluence using Lipofectamine 3000 (Invitrogen, #L3000015) in a 4:1 v/w ratio following manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: MA104-NSP5-EGFP cells (MA-NSP5-EGFP) (7) were cultured in DMEM supplemented with 10% FBS (Life Technologies), 50 μg/ml gentamycin (Biochrom AG ...
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bioRxiv - Cancer Biology 2019Quote: ... The RT-qPCR assays were completed using the ABI ViiA™ 7 sequence detection system (Applied Biosystems®) with the following parameters ...
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bioRxiv - Microbiology 2019Quote: ... Haematopoietic stem cells differentiated into BMDMs by incubating for 7 days in BMDM medium (1:1 SFM:DMEM, Gibco) supplemented with 10 % FCS (Biochrom) ...
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bioRxiv - Cancer Biology 2020Quote: ... Each murine sample was analyzed in triplicate on ViiA™ 7 Real-Time PCR System (Applied Biosystems®). Data generated was normalized to β-actin.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and Huh 7 engineering by lentivirus transduction to overexpress the human ACE2 (Huh-7hACE2) were cultured in Dulbecco’s modified 7 Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco). Working stocks of SARS-CoV-2 ICGEB-FVG_5 isolated in Trieste ...
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bioRxiv - Neuroscience 2021Quote: ... Forty cycles of amplification were run on a Quant Studio 7 Flex Real-Time PCR System (Applied Biosystems) and the resulting data was analyzed using the comparative ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... at a final DNA concentration of 0.4 ng/μl using the ViiA 7 Real Time PCR System (ThermoFisher). Amplification conditions were as follows ...
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bioRxiv - Genetics 2021Quote: ... and expression levels were quantified in the QuantStudio(tm) 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... reduced whole cell lysates were electrophoresed on a NuPAGE 7% Tris-Acetate pre-cast 1.0 mm gels (Invitrogen) for 50 minutes at 150 V and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
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bioRxiv - Biochemistry 2021Quote: ... ice cold PBS at 2e6 cells/ml and stained with 7-AAD viability dye (1 ug/mL, Invitrogen). Cell sorting was done on a FACSAria III instrument ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were concentrated in an Amicon Ultra 7 kDa filter and buffered exchanged into nuclease free water (Invitrogen), to which the indicated buffer and salt concentrations were added ...
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bioRxiv - Genomics 2021Quote: ... qPCR was performed on Viia 6/7 Real time PCR system using SYBR Green master mix (Life Technologies). The primers used are listed in Supplementary table 1 ...
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bioRxiv - Molecular Biology 2021Quote: ... HepG2 and Huh-7 cells were transfected with miRNA or siRNA using LipofectamineTM RNAiMAX transfection reagent (ThermoFisher Scientific) according to the manufacturer’s instructions and following experiments were performed 48 h after transfection ...
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bioRxiv - Biophysics 2021Quote: ... Cells were finally switched to serum-reduced medium comprising DMEM (HEK293T, COS-7) or FluoroBrite DMEM (Gibco, #A1896702) (HeLa) ...
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bioRxiv - Cell Biology 2021Quote: ... These experiments were carried out with the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). Expression of circRNAs was calculated relative to the housekeeping genes 18S or ACTB ...
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bioRxiv - Immunology 2021Quote: ... Whole bone marrow cultures were then cultured in 10 cm non-tissue culture treated dishes for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... bone marrow monocytes were immuno-magnetically isolated and culture for 7 days in Dulbecco’s modified Eagle’s medium (DMEM) containing 25 mM glucose (Invitrogen) and supplemented with 100 U/ml penicillin and 100 ng/ml streptomycin (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... polyclonal CD8+ T cells were labelled with 20 μM CellTracker™Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen) for 20-30 min at 37° C ...
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bioRxiv - Immunology 2021Quote: ... Real-time qPCR reactions were performed using the QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... siRNAs (3μl of 10μM conc.) and RNAiMax (7 μl) were mixed separately with 75 μl Optimem (Thermofisher, 31985062). Knockdown was initiated by mixing both siRNA and RNAiMAX suspensions together ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
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bioRxiv - Cell Biology 2022Quote: WT organoids passage 10 were collected 5-7 days after passaging and digested with TrypLE (Thermo Fisher Scientific) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at 7 days in vitro (DIV) using Lipofectamine 2000 (Thermo Fisher, Cat. no. 11668019). Experiments were carried out after 20-24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... with 7 to 14 cycles of PCR using the Phusion High-Fidelity PCR Master Mix (Thermo Fisher Scientific). The ctDNA yield was quantified with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Technical replicates for each experimental sample were analyzed in a ViiA 7 Real-Time PCR system (Applied Biosystems) using the standard curve method ...
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bioRxiv - Developmental Biology 2022Quote: ... together with its IgG2a kappa PE/Cyanine 7-Isotype control (1:500, Thermo Fisher Scientific, 25-4321-81), CD31-APC with human specificity for HUVEC experiments (1:500 ...
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bioRxiv - Cell Biology 2022Quote: COS-7 cells (ATCC CRL-1651) were cultured in DMEM (5.56 mM glucose, glutaMAX supplement, Thermo Fisher 10567022) supplemented with 10% fetal bovine serum (ThermoFisher 10438-034) ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: COS-7 cells were transiently transfected with the relevant DNA constructs using Lipofectamine 2000 (Invitrogen, catalog number 11668). The media was collected ...
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bioRxiv - Genomics 2022Quote: ... Reactions were carried out on a QuantStudio 7 Flex 384-well Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using 2xSybRGreen Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions of 7 to 12 representing the translation level were taken for RNA isolation with TRIzol reagent (Ambion). To perform RNA-seq experiment ...
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bioRxiv - Systems Biology 2022Quote: ... 4 × 108 cells per biological replicate were used after 7 days of blasticidin selection (10 mg/mL, Gibco), which was 9 days post-infection ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from 7 µl of RNA using the High-Capacity RNA-to-cDNA Kit (Thermo Fisher). TaqMan assays (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... was added to each well and amplification continued in a QuantStudio 7 Flex qPCR machine (Thermo Fisher Scientific) until signal plateaued out of exponential amplification using the following programme ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR assays were conducted using a QuantStudio 7 Pro Real Time PCR system from Thermo Fisher Scientific Inc ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we ran two technical replicate reactions in an QuantStudioTM 7 Flex Real-Time PCR System (Applied Biosystems™). We used 4μL of genomic template ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
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bioRxiv - Developmental Biology 2022Quote: ... Stained cell suspension was passed through a cell strainer and 7-aminoactinomycin D (Molecular probes by Life Technologies) was added for dead cell identification ...
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bioRxiv - Neuroscience 2023Quote: ... equal amounts of protein were loaded in 7% Bis-Tris gels and run with MOPS buffer (Thermo Scientific) and transferred to membranes (Amersham GE Healthcare) ...