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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Mouse Leptin ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Cytokines were quantified from 1 ml right lung homogenate using the ProcartaPlex Mouse 12-Plex Mix&Match kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... We simultaneously determined the concentrations of cytokines in supernatant samples using a customized 28-plex immunoassay kit (ProcartaPlex Mouse 28-plex, ThermoFisher Scientific), which employs magnetic beads for detection ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells from OT-I donors were enriched using the Dynabeads® Untouched™ Mouse T Cells kit (Invitrogen) and stained in 1ml DPBS with CellTrace Violet dye (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... goat-α-mouse-Alexa-488 and goat-α-mouse-Alexa-594 (Life Technologies). FISH was performed using a PNA probe specific to the telomeric sequence (CCCTAA)3 (TelC-Cy5 ...
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bioRxiv - Systems Biology 2024Quote: ... Incubations were performed with mouse anti-FLAG or mouse anti-V5 (ThermoFisher Scientific), and secondary anti-mouse IgG coupled with alkaline phosphatase (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and treated with 1x ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used were against mouse (Donkey anti-mouse, AF488; Invitrogen Cat#A21202) or goat (Donkey anti-goat AF488 ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Animal Behavior and Cognition 2019Quote: Serine protease inhibition assays were performed at 37°C by using a thermostated Multiskan GO ELISA plate reader (Thermo Scientific). Serine proteases and their corresponding substrates (in brackets ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed 3 times with PBS-T and then 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were assayed for IL-2 or IFNγ by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Thermo fisher).
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bioRxiv - Biochemistry 2021Quote: ... This was followed by the plate wash step and an incubation with 1-Step Ultra TMB-ELISA (Thermo Scientific, SG) at 100 μL/well for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific, 43028) and quenching with 1N HCl ...
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bioRxiv - Microbiology 2020Quote: ... all peptides at a final concentration of 0.5 μg/mL in 50 mM pH 9.6 Na2CO3/NaHCO3 buffer were coated on ELISA plates (Nunc, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... a solution of 1% baculovirus particles in 100mM sodium bicarbonate buffer pH 9.6 was absorbed onto the wells of a 384-well ELISA plate (Nunc Maxisorp) using a Tecan Freedom Evo liquid handling robot ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed again three times and developed using a SuperSignal™ ELISA Pico Chemiluminescent Substrate (ThermoFisher; Cat#37069) and the level of luminescence detected using a Cytation 3 image reader luminometer using Gen5 software.
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... washed thrice in PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (Thermo Fisher Scientific, Cat. #34028). Following a 12 to 15-min incubation ...
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bioRxiv - Immunology 2020Quote: ELISAs were performed using 50 ng of VP1 pentamer/well in an EIA/RIA Polystyrene High Bind Microplate (Fisher Scientific) coated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... gH/gL or gL (diluted in PBS; 100 µl/well) were coated overnight on Nunc MaxiSorp high protein-binding capacity ELISA plates (ThermoFisher). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... plates were washed thrice with PBST and 50 µL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 12 to 15-min incubation ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: Medium samples from cells and from the engrafted scaffolds were used to measure TGFβ using ELISA (88-8350-22, ThermoFisher), following manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... submandibular bleeding was performed to collect blood for measurement of serum IL-1β levels by ELISA (Invitrogen 88-7013-22). At 4 hours ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Microbiology 2022Quote: ... To measure the protein concentration of IFN-λ1 and -λ2 present in the SN we performed an ELISA following the protocol from the manufacturer: IFN-λ1 (IL-29) from Invitrogen/Thermo Fisher Scientific cat ...
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bioRxiv - Molecular Biology 2022Quote: ... the phage enrichment was evaluated by pooled phage ELISA in 384-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark). Bait proteins and GST control were immobilized (5 μg in 50 μl PBS per well ...
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bioRxiv - Cell Biology 2022Quote: ... HMGB1 was measured in cell-free supernantants using an enzyme-linked immunosorbent assay (Tecan HMGB1 ELISA, cat# ST51011, Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... pH 7.2 (PBS) and 100 ul added to wells of a 96-well medisorp ELISA plate (Thermo Fisher Scientific #467320) and incubated overnight at 4° C ...
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bioRxiv - Systems Biology 2024Quote: ... We detected proteins by chemiluminescence using the SuperSignal® ELISA femto maximum sensitivity substrate (Thermo Fisher Scientific™, Waltham, USA) according to the manufacturer’s instructions and the Amersham™ Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies ...
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bioRxiv - Pathology 2024Quote: ... and C-X-C Motif Chemokine Ligand 1 (CXCL1) levels were measured in liver protein lysates and EDTA-plasma by enzyme-linked immunosorbent assay (ELISA) (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... deglycosylated GCase and the GCase N370S mutant were solid-phase coated on the bottom of a 96-well ELISA plates (Maxisorp Nunc-immuno plate ...
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bioRxiv - Bioengineering 2024Quote: ... the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10 ...
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bioRxiv - Immunology 2024Quote: Sera competition ELISA assay plates were coated with PBS containing 250 ng of anti-6x His tag monoclonal antibody (Invitrogen) and left covered overnight at 4°C ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488/555/647 anti-rabbit IgG, anti-mouse IgG1, anti-mouse IgG2a, anti-mouse IgG2b, or anti-goat IgG; 1:1,000, Invitrogen, Grand Island, NY, USA). Nuclei were counterstained with DAPI (#62248 ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated and purified from cultured mouse TPCs (passage 1) with Trizol followed by complementary DNA synthesis with RevertAid™ H Minus kit (#K1632 Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from spleen and mesenteric lymph nodes single cells suspensions by positive separation using a Dynabeads FlowComp Mouse CD4 Kit (11461D, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: RNA was prepared from total mouse brains of mice and from BECs using Trizol and Cells-to-CT kit (Thermo Fisher Scientific), respectively ...
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bioRxiv - Microbiology 2022Quote: Lysates extracted from mouse lung tissues with a bead shocker were processed and digested using an EasyPep Mini MS Sample Prep Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... ME) using the EasySep™ mouse T cell isolation kit (StemCell, Cambridge, MA) and labeled with carboxyfluoroscein succinimidyl ester (CFSE; Life Technologies) as described previously [18] ...
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bioRxiv - Immunology 2021Quote: ... Insertion of single base pair changes for the generation of human and mouse FOXN1 variants was achieved by site-directed mutagenesis using the Phusion site-directed mutagenesis kit (Thermo Fisher Scientific).Specific primers for the introduction of single base pair deletions or single base pair changes were designed using the following software www.agilent.com/store/primerDesignProgram.jsp ...
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bioRxiv - Developmental Biology 2020Quote: ... biotinylated with Ovation kits from NuGEN (San Carlos, CA) and hybridized to the Affymetrix mouse genome 430 2.0 array (Affymetrix, Santa Clara, CA). Each array was probed with RNA isolated from an individual mouse ...
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bioRxiv - Immunology 2021Quote: T cells were enriched from the lymph nodes and spleen of mice using the Dynabeads untouched mouse T cell kit (Thermo Fisher Scientific) or the CD4+ T cell selection kit (Miltenyi) ...
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bioRxiv - Neuroscience 2022Quote: ... > 8.0 were further processed with the Affymetrix WT Plus kit and hybridized to Clariom S mouse arrays as described by the manufacturer (Thermo Fisher, Germany). Briefly ...
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bioRxiv - Immunology 2022Quote: ... RNA-seq libraries were prepared with 1 ng of total RNA using an Ion AmpliSeq Transcriptome Mouse Gene Expression kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... TNF-α and IL-5 cytokines using a mouse cytokine 11-plex antibody bead kit (Th1/Th2 Cytokine 11-Plex Mouse ProcartaPlex™ Panel, Cat No# EPX110-20820-901, Thermo Fisher). Preparation of samples ...