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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Mouse Leptin ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... the plate was washed six times and developed with 1-Step™ TMB ELISA Substrate Solutions (Thermo Fisher Scientific). The absorbance was measured at 450 nm using a SpectraMax M2 microplate reader (Molecular Devices) ...
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Immunology 2021Quote: ... Cytokine and chemokine analysis from organ lysates and plasma were performed with the mouse 36-plex ProcartaPlex cytokine/chemokine array kit (ThermoFisher Scientific), G-CSF mouse or human ProcartaPlex Simplex Kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... splenic B cells were isolated from splenocytes using the Dynabeads™ Mouse CD43 Untouched™ B Cells kit (11422D, Thermo Fisher), per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems) as described previously (21) ...
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bioRxiv - Microbiology 2022Quote: ... mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100 ...
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bioRxiv - Systems Biology 2019Quote: ... whereas the poly(A)-tailed RNA in yeast cells and mouse tissues was isolated from the total RNA using a Dynabeads mRNA Purification kit (Life Technologies) following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2019Quote: Murine bone marrow cells from the femur and tibia were depleted of lineage positive cells using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, StemCell Techologies, Vancouver, Canada) and cultured overnight in DMEM (Gibco) media supplemented with 15% FBS (Gibco) ...
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bioRxiv - Immunology 2020Quote: 1,000 naive GFP-expressing OT-I cells were purified from a TgTcraTcrb1100Mjb;Rag2−/−;Ubc-GFP+/+ spleen with the Dynabeads Untouched Mouse CD8 Cells Kit (ThermoFisher Scientific) and transferred i.v ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Bioengineering 2022Quote: ... RGCs were isolated from freshly dissociated organoids by magnetic microbead sorting for Thy1.2+ cells using the Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit (Invitrogen, 11465D) according to the manufacturer protocol.
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bioRxiv - Neuroscience 2022Quote: ... RNA samples with RNA integrity numbers (RIN) > 8.0 were further processed with the Affymetrix WT Plus kit and hybridized to Clariom S mouse arrays (ThermoFisher, Cat# 902931) as described by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: Western blot analysis was conducted using cytoplasmic extracts from mouse brain cells made with a NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher). The mixture was centrifuged at 10,000□×□g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from HeLa cells (human) or melanocytes (mouse) melan-Ink4a (Shakya et al., 2018) was isolated using a GeneJET RNA purification kit (ThermoFisher Scientific) in the presence of β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... We simultaneously determined the concentrations of cytokines in supernatant samples using a customized 28-plex immunoassay kit (ProcartaPlex Mouse 28-plex, ThermoFisher Scientific), which employs magnetic beads for detection ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells from OT-I donors were enriched using the Dynabeads® Untouched™ Mouse T Cells kit (Invitrogen) and stained in 1ml DPBS with CellTrace Violet dye (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Cytokines were quantified from 1 ml right lung homogenate using the ProcartaPlex Mouse 12-Plex Mix&Match kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: RNA Isolation: RNA extraction and isolation of mouse podocytes was performed using the PicoPure RNA isolation Kit (Thermo Fisher Scientific, #KIT0204) according to manufacturer’s protocol (100 µL instead of 50 µL of lysis buffer was applied to extract RNA) ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated from cultured mouse neurons treated with xcTauOs (250 nM total tau) or vehicle for 6 hours using the mirVana isolation kit (Invitrogen, AM1560). RNA integrity was evaluated by capillary electrophoresis using an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... ribosomal RNA was depleted from the samples using the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific, Waltham, MA). All samples were sequenced on an Illumina Hi-Seq sequencer to produce 150 bp paired-end reads ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... from 150 μl plasma (single mouse or pooled) and the TG and cholesterol content were measured using colorimetric kits (Inifinity, Thermo Scientific, TG catalog #TR22421 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked again and incubated with 2-3 drops of an anti-mouse poly-HRP-conjugated secondary antibody from the Invitrogen Tyramide SuperBoost secondary staining kit (ThermoFisher, B40912). Samples were rinsed three times with 1X PBS and the tyramide working solution conjugated to AlexaFluor 488 was added and allowed to react for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched using negative magnetic selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D).
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bioRxiv - Neuroscience 2023Quote: Mouse embryonic fibroblast (MEF) cell lysates were processed using the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842) following the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Initial isotyping of the antibodies in hybridoma supernatants was carried out using Pierce Rapid Antibody Isotyping Kits for mouse (Thermo Fisher). Antibodies were subsequently used as confluent supernatants for most techniques ...
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bioRxiv - Microbiology 2019Quote: ... goat-α-mouse-Alexa-488 and goat-α-mouse-Alexa-594 (Life Technologies). FISH was performed using a PNA probe specific to the telomeric sequence (CCCTAA)3 (TelC-Cy5 ...
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bioRxiv - Systems Biology 2024Quote: ... Incubations were performed with mouse anti-FLAG or mouse anti-V5 (ThermoFisher Scientific), and secondary anti-mouse IgG coupled with alkaline phosphatase (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and treated with 1x ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution (Invitrogen) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used were against mouse (Donkey anti-mouse, AF488; Invitrogen Cat#A21202) or goat (Donkey anti-goat AF488 ...
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bioRxiv - Developmental Biology 2019Quote: ... stained with fluorophore-labeled secondary antibodies (Alexa 488 Donkey anti-Mouse, Alexa 555 Donkey anti-Mouse, Alexa 647 Donkey anti-Mouse, Alexa 488 Goat anti-Rabbit; Invitrogen, Carlsbad, CA) and DAPI (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Coronal sections 50 um thick were immunostained for tyrosine hydroxylase (primary: mouse anti-TH, Millipore #MAB318; secondary: goat anti-mouse 647, Invitrogen #A-21236 or goat anti-mouse 405, Invitrogen #A-31553), tryptophan hydroxylase 2 (primary ...
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bioRxiv - Cell Biology 2020Quote: Whole protein extracts were prepared from JJN3 pellets (4°C, 150 x g, 5 min) according to the cell extraction protocol for ELISA sample preparation by ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: Concentrations of IFN-λ1 in basolateral medium samples from WD-PNECs were measured using a human IFN-λ1/IL-29 ELISA (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Animal Behavior and Cognition 2019Quote: Serine protease inhibition assays were performed at 37°C by using a thermostated Multiskan GO ELISA plate reader (Thermo Scientific). Serine proteases and their corresponding substrates (in brackets ...
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bioRxiv - Immunology 2021Quote: ... Samples were washed 3 times with PBS-T and then 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were assayed for IL-2 or IFNγ by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (Thermo fisher).
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bioRxiv - Biochemistry 2021Quote: ... This was followed by the plate wash step and an incubation with 1-Step Ultra TMB-ELISA (Thermo Scientific, SG) at 100 μL/well for 3 min ...
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bioRxiv - Biochemistry 2020Quote: ... 5 washes of TBST were done followed by development using 1-Step Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific, 43028) and quenching with 1N HCl ...
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bioRxiv - Microbiology 2020Quote: ... all peptides at a final concentration of 0.5 μg/mL in 50 mM pH 9.6 Na2CO3/NaHCO3 buffer were coated on ELISA plates (Nunc, Roskilde, Denmark) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... a solution of 1% baculovirus particles in 100mM sodium bicarbonate buffer pH 9.6 was absorbed onto the wells of a 384-well ELISA plate (Nunc Maxisorp) using a Tecan Freedom Evo liquid handling robot ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed again three times and developed using a SuperSignal™ ELISA Pico Chemiluminescent Substrate (ThermoFisher; Cat#37069) and the level of luminescence detected using a Cytation 3 image reader luminometer using Gen5 software.
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bioRxiv - Immunology 2021Quote: ... Murine IL-2 ELISA capture (catalog no. 14-7022-85) and detection (catalog no. 13-7021-85) antibody reagents were purchased from Invitrogen/eBioscience ...
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bioRxiv - Microbiology 2020Quote: ... washed thrice in PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (Thermo Fisher Scientific, Cat. #34028). Following a 12 to 15-min incubation ...
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bioRxiv - Immunology 2020Quote: ELISAs were performed using 50 ng of VP1 pentamer/well in an EIA/RIA Polystyrene High Bind Microplate (Fisher Scientific) coated overnight at 4°C ...