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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Mouse Leptin ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... CRP and IL-6 were measured in UCB plasma using a high-sensitivity ELISA (Life Technologies, Carlsbad CA) per the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... and the assay was developed with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher, Cat#34029) and blocked with 2N H2S04 solution before reading ...
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bioRxiv - Immunology 2022Quote: ... 200 ng/ml) and medium was collected for analysis of the amount of secreted TNFα by ELISA (Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The concentration of Aß40 and Aß42 in the culture medium was quantified by ELISA (Thermo Scientific, Waltham, MA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were collected for cytokine analysis by ELISA (IL-2 BD-OptEIA™; IL-17A, IFN-γ ThermoFisher) following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Immunology 2022Quote: ... the plate was developed using 50 µl of 1-StepTM Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well ...
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bioRxiv - Neuroscience 2023Quote: ... the substrate of the peroxidase (100µL, 3,3’,5,5’-tetramentylbenzidine solution; 1-StepTM Ultra TMB-ELISA, 34029, Thermo Scientific) was added in each well for 10 to 25 minutes at RT and in darkness ...
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bioRxiv - Bioengineering 2023Quote: ... 2ug/mL of SARS CoV2 BA.1 RBD protein were coated on 96-well ELISA plates (Nunc Maxisorp) and left overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: Total IgA and IgG ELISAs were performed using 384-well flat-bottom MaxiSorp plates (ThermoFisher, Cat No. 464718) coated with either an unconjugated anti-human IgA (Novusbio ...
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bioRxiv - Immunology 2023Quote: ELISA plates (Microtitre) were coated with 50 µl/well of 5 µg/ml NeutrAvidin (Thermo Fisher Scientific, #31000) for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed five times and developed with tetramethylbenzidine (1-Step Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrations of IL33 and periostin in culture supernatants were measured by ELISA according to the manufacturer’s instructions (Invitrogen, cat # ...
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bioRxiv - Microbiology 2023Quote: ... Plates were developed with 1-Step ultra TMB-ELISA substrate solution (Thermo Fisher Scientific, Waltham, MA, USA, #34028) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
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bioRxiv - Immunology 2023Quote: ... the ELISA plates were incubated with horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (1:5000, Invitrogen) for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The concentrations of human α-synuclein were measured using a human α-syn-specific ELISA (ThermoFisher Scientific #KHB0061) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were then enriched via negative selection using Dynabeads Untouched Mouse CD4 T cells Kit (Thermo Fisher Scientific) according to manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... mice which were maintained on tamoxifen diet for 4-5 wks and from CD45.1 mice (as described above) which were subjected to T cell depletion using Dynabeads FlowComp Mouse Pan T (CD90.2) Kit (ThermoFisher Scientific). BM cells from WT or FL mice were mixed with the BM of the competitor donor at 1:1 ratio and then transplanted by intravenous injection into lethally irradiated CD45.1 mice (900 rad) ...
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bioRxiv - Microbiology 2020Quote: Luminex profiling was performed on whole blood that was allowed to clot for 20 minutes and then spun down using a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and then stained with triple-washed anti-IgG magnetic beads (Untouched Mouse T Cells Kit, Thermo Fisher) rotating at 4°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: Using a subcloned and sequenced PCR fragment (162 bp – Table S1) of the mouse 5-HT2AR cDNA (Zero Blunt TOPO PCR Cloning kit, Invitrogen), high specific-activity RNA probes were produced from a linearized plasmid (HindIII ...
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bioRxiv - Microbiology 2022Quote: Chemokine and cytokine profiles of serum and lung samples were assessed using Immune Monitoring 48-plex mouse ProcartaPlex Panel kits (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... containing a 1/250 dilution of Alexa 488-conjugated anti-Spike antibody (GTX632604 conjugated using the Zenon Alexa Fluor 488 Mouse IgG Labeling Kit, ThermoFisher) and washed 4 times in FACS buffer ...
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bioRxiv - Physiology 2021Quote: ... RNA samples were prepared from the mouse tissues using the PureLink RNA Mini Kit (Invitrogen by ThermoFisher Scientific, catalog #12183025). Briefly ...
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bioRxiv - Immunology 2020Quote: Peripheral T cells were isolated from mouse spleen by a CD4+ T-cell negative (Stem Cell) or positive selection kit (Invitrogen). Enriched CD4+ T cells were further sorted for either YFP+ (Foxp3+ ...
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bioRxiv - Neuroscience 2020Quote: ... were designed against the mouse Auts2 coding sequence using the BLOCK-iT Pol II miR RNAi Expression Vector Kit guidelines (Invitrogen). Auts2 miRNA constructs were subcloned into the pCL20c-trL7 ...
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bioRxiv - Immunology 2020Quote: Splenic T cells from MRL/Lpr mice were purified with Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit and dissocated from beads as per manufacture’s instructions (ThermoFisher). Purified T cells were staind with PE-conjugated anti-CD138 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... For direct labeling of the anti-CC10 antibody Zenon Alexa Fluor 546 mouse IgG1 kit was used according to the manufacturer’s recommendations (Invitrogen, 25004). Stained cryosections were analyzed on an epifluorescence microscope (Zeiss Imager.M2 ...
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bioRxiv - Immunology 2021Quote: ... S98I to the SIRPα domain which was then subcloned and fused to mouse IgG1 N297A via GeneArt Seamless Plus Cloning Assembly Kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Barcoded libraries were prepared using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit as per the manufacturer’s protocol and sequenced using an Ion S5 system (ThermoFisher Scientific). Differential gene expression analysis was performed using the Transcriptome Analysis Console (TAC ...
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bioRxiv - Immunology 2021Quote: MesLNs were collected and CD4+ T cells isolated using the Dynabeads™ Untouched™ Mouse CD4 Cells Kit (Thermo Fisher) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... naïve OT-I and OT-II cells were obtained by negative selection using the Dynabeads Untouched Mouse CD4 cell kit (ThermoFisher). Flow cytometry indicated >85% enrichment in all cases ...
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bioRxiv - Immunology 2021Quote: MRL/Lpr mice splenic T cells were purified with Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit and dissociated from beads according to manufacturer’s instructions (ThermoFisher). After staining purified T cells with PE anti-CD138 antibody ...
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bioRxiv - Immunology 2022Quote: ... CD4+CD25+ natural Tregs were isolated from the splenocytes using the DynabeadsFlowComp Mouse CD4+CD25+ Treg cells Kit (11463D, Invitrogen) following the manufacturer’s instructions and as previously described (37)(38) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from either cultured neurons (>1.5×106) or mouse spinal cord tissue (10-20 mg) using the PureLink RNA Mini kit (Thermo Scientific) and treated with DNase I (NEB ...
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bioRxiv - Cancer Biology 2023Quote: 20 μg protein per sample was used for the IFN-gamma mouse ProcartaplexTM Simplex High Sensitivity kit (Thermo Fisher Scientific) and the protocol was followed as per the product manual ...
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bioRxiv - Physiology 2023Quote: ... was incubated with 5 µl Zenon component A solution for five minutes and then with 5 µl Zenon component B solution (ZenonTM Mouse IgG1 Labeling Kit Z-25006, Alexa Fluor 568, Invitrogen). PBS with 0.1% triton was added to the Zenon-labeled antibody mix to a final 1:75 dilution ...
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bioRxiv - Cell Biology 2023Quote: A PCR product encoding mouse RAB18 was subcloned into an intermediate TOPO vector using a TOPO PCR Cloning Kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... both following manufacturer’s suggested protocols for the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (Thermo Fisher Scientific, San Jose, CA). Differentially expressed genes (DEGs ...
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bioRxiv - Physiology 2023Quote: First and second-order mesenteric arteries from each mouse were pooled and placed into lysis buffer from the RNAqueous Micro Kit with DNase from Ambion Inc ...
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bioRxiv - Cell Biology 2023Quote: ... commercially available kits were purchased and the assays done following the manufacturer’s protocol: IL-1β (Mouse: BMS6002; Human: BMS224INST, Thermo Fisher) and HMGB1 (Mouse ...
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bioRxiv - Immunology 2023Quote: ... Mouse FM expression of IL1B and KC was measured by RT-qPCR using the SuperScript II kit (Invitrogen; Waltham, MA) and the Kapa SYBR Fast qPCR kit (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... We followed the kit protocol except for conjugating 5µg of anti-total RNAPII to sheep anti-mouse dynabeads (Life Technologies). Sequencing libraries were generated using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina.
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched from homozygous TCR-transgenic OT-II (CD45.1/.2) donors using the Dynabeads Untouched™ Mouse CD4 Cells kit (Invitrogen). 1 x 105 OT-II cells were transferred intravenously (i.v. ...
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bioRxiv - Physiology 2020Quote: ... βTC3 cells were transfected with plasmids encoding mouse furin and/or mouse insulin receptor (pcDNA3.1 backbone) and/or mouse Atp6ap1/Ac45-Flag using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... for more than 1.5 hours in a shaker and incubated with the primary antibody (Mouse anti- GAD67, Millipore; Mouse anti-CaMK2a, Abcam; Mouse anti-mCherry, Invitrogen) in 0.2% Triton and 5% Goat serum in PBS at 4 °C for 24 -36 hours ...