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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Mouse Leptin ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... The antibodies were diluted to 1 µg/ml in PBS and 100 µl of this solution per well were applied to the Nunc MaxiSorp ELISA plate (Thermo Fisher) for coating overnight ...
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bioRxiv - Immunology 2019Quote: ... 50 μL of HLA binding neutralisation buffer was added to each well and the resulting supernatants were incubated for 2 hours at room temperature in ELISA plates (Nunc, Denmark) previously coated with 10 μg/mL of the monoclonal antibody L243 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1/512) and 50μl of each dilution was added to Immulon™ 2 HB flat bottom 96-well ELISA plates (Thermo Scientific) in triplicate ...
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bioRxiv - Microbiology 2021Quote: ... The peroxidase reaction was terminated by adding 50 μL of H2SO4 (1 M) before the final optical density (OD) was taken using an ELISA plate reader (Multiskan Go, Thermo Scientific) at the wavelength of 450 nm ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was then washed several times with 250 μl of PBS-0.05% Tween-20 and incubated with 100 μl of SuperSignal ELISA Pico chemiluminescent substrate (Thermo Scientific #37069). The resulting signal was read 10 minutes post addition of the substrate For quantification ...
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bioRxiv - Molecular Biology 2020Quote: ... The washing procedure was repeated and 50 μL of the buffer containing substrate (1-Step Ultra TMB ELISA Substrate, Thermo Scientific) was added to develop the colorimetric reaction for 15 min ...
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bioRxiv - Microbiology 2021Quote: CVL supernatants were recovered by centrifugation for assessment of IL-8 levels by Enzyme-Linked Immunosorbent Assay (ELISA) (Invitrogen, Paisley, UK), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Determination of SARS CoV-2 spike protein-specific human IgG antibody titers was performed in Maxisorp Immuno 96-well ELISA plates (Thermo Scientific) coated overnight at 4°C with 100 μl/well of 2 μg ml−1 recombinant SARS CoV-2 spike protein produced and purified from 293 cells in PBS ...
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bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
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bioRxiv - Microbiology 2019Quote: ... A final washing step after 1 h incubation at TR was followed by addition of 100 µl 1-Step Slow TMB-ELISA substrate (Thermo Scientific) to each well and absorbance was measured after 10 min incubation at TR using the Bio-Rad Model 680 Microplate Reader (655 nm ...
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bioRxiv - Synthetic Biology 2020Quote: ... were used for overnight coating of individual wells in a MaxiSorp high protein-binding capacity 96-well ELISA plate (Nunc, Denmark) at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 48 h and cytoplasmic DNA was extracted according to the protocol as previously described.35 The cytoplasmic DNA lesion and plasma levels of DNA damage were measured using the DNA Damage Competitive ELISA (Thermo Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2021Quote: ... plates were washed thrice with PBS-T and 20 μL of freshly prepared SuperSignal ELISA Pico Chemiluminescent Substrate (Thermo Scientific, #37069) (mixed 1:1 ratio and diluted in equal volume with dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... ELISA wells were washed four times with PBS-T and incubated with 50 μL/well of anti-GST (Invitrogen MA4-004) or anti-MAR (Millipore-Sigma MAB1076 ...
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bioRxiv - Immunology 2021Quote: ... Cell culture supernatants were collected for ELISA assays and RNA was extracted from infected cells using Trizol (ThermoFisher; catalog no. 15596026) following manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Plates were washed 5 times with washing buffer and developed by addition of 100µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific) for 3 min ...
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Immunology 2020Quote: ... The MMP Luminex panel (PPX-05/ PROCARTAPLEX MMP1, MMP2, MMP3, MMP9 and MMP13 plex) and IL-6 Elisa (both from Life Technologies) was performed on co-cultures supernatants.
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bioRxiv - Microbiology 2021Quote: ... diluted 1:3000 in 1% PBS-milk supplemented with 0.1% Tween-20 for 1 h at 37C and detected using 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific). Plates were read using a Cytation 5 imager (BioTek ...
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bioRxiv - Microbiology 2020Quote: Rabbit serum immunized with the DENV-2 NGC strain was coated onto Nunc Maxisorp ELISA plates (Thermo Fisher Scientific, Waltham, MA). Then ...
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bioRxiv - Microbiology 2022Quote: ... plates were washed 3 times and then developed by adding 100 µl/well of TMB-ELISA substrate (ThermoFisher Scientific, catalog# 34029). Reactions were stopped after 10 minutes by adding 50 µl/well of 3M H2SO4 to all the wells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Venom was diluted in 100 mM carbonate-bicarbonate coating buffer pH 9.6 and coated at 100 ng per well in 96-well ELISA plates (Nunc MaxiSorp, ThermoScientific) and incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... content in isolated mitochondria from WT and APP cells was measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer instructions (ThermoFisher khb3544).
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bioRxiv - Microbiology 2022Quote: IgG binding to SARS-CoV-2 or MeV antigens was measured by ELISA using clear flat-bottom immuno nonsterile 96-well plates (Cat# 442404, ThermoFisher Scientific) coated overnight at 4°C with 100 ng of recombinant SARS-CoV-2 proteins or 1 μg of MeV bulk antigen (Cat# BA102VS ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 RBD antigens expressed in 293T cells were used to coat the 96-well ELISA plates (Cat No. 44240421, Thermofisher, USA). 50µL (2µg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... except for antibodies used for detection in ELISA and flow cytometry. Genetic constructs were synthesized by Eurofins Genomics. Plasmid DNA midiprep (cat. K210004) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Using the standard curve from the ELISA and the amount of gDNA/sample (quantified by Nanodrop Spectrophotometer from Thermo Fisher Scientific), percentage of methylated DNA (5-mC ...
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was taken at 590 nm with a reference filter of 620 nm using an ELISA reader (Multiskan Spectrum Microplate Reader, Thermo Scientific). All data were normalized against DMSO blank controls.
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bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) at 2 µg/mL were coated onto 96 well ELISA plates (Immulon 2 HB, Thermo Fisher) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... plates were washed 3 times with PBST and added with 50 μL of 1-Step Ultra TMB-ELISA (ThermoFisher, Cat. #34028). Reactions were stopped by the addition of 50 μL of 2 M sulfuric acid ...
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bioRxiv - Microbiology 2024Quote: ... plates were washed 3 times with PBST and 50 μL of 1-Step Ultra TMB-ELISA was added (ThermoFisher Cat. #34028). Following a 2 to 5-min incubation ...
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bioRxiv - Neuroscience 2024Quote: ... Triton- and Guanidine-soluble samples were subjected to analysis using species- and isoform-specific antibodies in Soluble and Insoluble Aβ ELISA (Invitrogen, Life Technologies; Grand Island ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were probed with mouse anti-HA (12CA5) and mouse anti-Pgk1 (22C5D8, Molecular Probes), followed by probing with mouse anti-Pgk1 (22C5D8 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse primary antibody diluted 1:10,000 and anti-mouse-HRP secondary antibody (Thermo Fisher Scientific) diluted 1:50,000.
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 555 Mouse and Alexa Fluor 488 Mouse secondary antibodies were obtained from Invitrogen. The polyclonal antiserum to the recombinant human megalin cytoplasmic domain (anti-megT ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The probes for mouse TNF-α (Mm00443260_g1) and mouse β-actin (Mm00607939_s1) (Thermo Fisher Scientific) were used ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse IgG/IgM AlexaFluor 488 and anti-mouse IgG/IgG2α AlexaFluor 546 (both Invitrogen) were used ...
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bioRxiv - Immunology 2022Quote: ... and anti-pp65 antibody (mouse, Argene) combined with goat anti-mouse antibody (AF488, Thermo Fisher), respectively.
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bioRxiv - Genetics 2020Quote: ... cells were incubated in secondary antibodies Mouse Texas Red X (ThermoFisher, anti-mouse, 1:500) and Cy5 (anti-rabbit,1:500) ...
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bioRxiv - Immunology 2022Quote: ... mouse-anti-mouse/human/rat monoclonal PP1a (1:1000 dilution, number MA5-17239, ThermoFisher Scientific), rabbit-anti-Y.pestis polyclonal YopM (1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... Alex Fluor 647 goat anti-mouse and Alexa Fluor 594 goat anti-mouse from Invitrogen. All secondary antibodies were used at a concentration of 1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti 6xHis (mouse monoclonal, HIS.H8, Thermo Fisher Scientific, MA1-21315; WB 1:2000), anti GFP (mouse monoclonal ...
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bioRxiv - Genomics 2023Quote: ... EY.T434 mouse embryonic fibroblast cells mouse embryonic fibroblast cells were grown in DMEM (ThermoFisher 10566016) supplemented with 15% (vol/vol ...
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bioRxiv - Immunology 2024Quote: ... and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were obtained from Thermo Scientific. BHA (Cat# B1253 ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 350 goat anti-mouse and Alexa Fluor 488 goat anti-mouse IgG (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... in PBS1X containing MOM reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution, Invitrogen, R37621) at RT ...
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bioRxiv - Microbiology 2020Quote: ... Slides were washed with PBS and incubated for four hours with anti β-actin antibody labelled with 594 fluorophore using the Zenon Mouse IgG Labeling Kit (Thermo Fisher Scientific). Dilutions of primary and secondary antibodies were the same as the ones used for the staining of human cells described above ...