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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Mouse Leptin ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... OSP and CT ELISAs were then blocked with warm 200 µl/well of R10 media (RPMI + L-Glutamate (Gibco), 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... in carbonate buffer (carbonate buffer 15 mM pH 9.6) was coated overnight at 4°C in 96 well ELISA plates (Nunc). Wells were then blocked with 5% dry milk for 2h at room temperature ...
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bioRxiv - Immunology 2019Quote: Serum IL-6 and IL-17A concentrations were assessed by ELISA according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: The concentration of cytokines in the 24h and 48h supernatants from the T57 T cell activation assay was measured by ELISA according to the manufacturer’s instructions (IFNγ: Invitrogen eBioscience, cat#88731477, IL-2: Invitrogen eBioscience ...
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bioRxiv - Immunology 2020Quote: The concentration of cytokines in the 24h and 48h supernatants from the T57 T cell activation assay was measured by ELISA according to the manufacturer’s instructions (IFNγ: Invitrogen eBioscience ...
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bioRxiv - Microbiology 2019Quote: ... After 4 washes with PBS 0.01% v/v Tween-20 and 2 washes with ELISA Light washing buffer (ThermoFisher), CSPD substrate with Sapphire II enhancer (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Cytokine concentrations were measured by TM ELISAs according to the manufacturer’s instructions in Nunc MaxiSorp flat-bottom plates (Invitrogen) using Uncoated ELISA Kits (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The color reaction was started by adding 100 μL of One-step Ultra TMB ELISA substrate (Thermo Scientific #34028) was added to each well (100 μL) ...
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bioRxiv - Immunology 2021Quote: ... was coated onto each wells of Nunc maxisorp high protein-binding 96 well ELISA plate (Thermo Fisher Scientific Inc.) and incubated overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... slgA concentrations in saliva were measured using the ready-set-go ELISA system for human IgA (Affymetrix: eBioscience, USA) with a sample dilution of 1:7500 at the University Hospital RWTH Aachen (SI ...
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bioRxiv - Immunology 2021Quote: ... The hACE-2-Fc content in the supernatant was quantified by ELISA using 96-Well MaxiSorp Immuno plates (ThermoFisher) coated overnight with 200 ng/well of RBD219-WT protein ...
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bioRxiv - Bioengineering 2022Quote: ... Protocols provided by the manufacturer were adapted to allow ELISAs to be performed in a 384-well plate (ThermoFisher) to minimize medium (sample ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL/well of 1-step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, cat. # PI34029) was added to the plates and incubated for 5 to 10 min at RT ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plates were washed three times and then developed using 50 μL 1-Step Ultra TMB-ELISA substrate solution (ThermoFisher). A similar ELISA protocol was followed for detecting RNC complexes displaying glycosylated DQNATpd except that the plates were coated with 50 μL of 5-μg/mL mouse mAb FB11 in PBS and were subsequently detected with HRP-conjugated anti-mouse IgG.
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were collected for cytokine profiling by quantitative ELISA for IFNγ and IL-17A as per manufacturer’s instructions (Invitrogen). Prior to flow cytometric analysis for cytokine production ...
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bioRxiv - Biochemistry 2023Quote: ... the plate was developed using 50 µl of 1-Step TMB Turbo-TMB-ELISA Substrate Solution (Thermo Fisher Scientific) per well ...
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bioRxiv - Pathology 2023Quote: ... CCL2 levels were measured in conditioned media by ELISA (Invitrogen, cat nos. 88-7391-88 and 88-7399-88).
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bioRxiv - Biochemistry 2023Quote: ... followed by the development of signal by treating cells with 200 μl TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue colour appeared ...
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bioRxiv - Bioengineering 2023Quote: ... Plates were extensively washed six times and incubated with the optimized volume of Ultra TMB- ELISA Substrate Solution (ThermoFisher) for 20 min ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Microbiology 2024Quote: ... The absorbent (A450) was measured by the plate reader after HRPO substrate (1-Step Ultra TMB-ELISA, ThermoFisher Scientific) was ...
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bioRxiv - Molecular Biology 2022Quote: Resting primary B and T cells were isolated from mouse spleens by negative selection using Mouse CD43 (Untouched™ B Cells) and Untouched Mouse T Cells Dynabeads respectively (Invitrogen). For experiments done in quiescent splenocytes ...
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Immunology 2021Quote: ... Cytokine and chemokine analysis from organ lysates and plasma were performed with the mouse 36-plex ProcartaPlex cytokine/chemokine array kit (ThermoFisher Scientific), G-CSF mouse or human ProcartaPlex Simplex Kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... splenic B cells were isolated from splenocytes using the Dynabeads™ Mouse CD43 Untouched™ B Cells kit (11422D, Thermo Fisher), per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... We generatead F(ab’)2 fragments using Pierce™ Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher Scientific #44980). ROS production upon stimulation with or without 10 μM peptide S100A6-1 coated to a Microfluor® 2 white-bottom 96-well plate (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... and GAPDH was detected as an endogenous control using the Pre-Developed TaqMan Assay Reagent kit for mouse GAPDH (Applied Biosystems) as described previously (21) ...
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bioRxiv - Microbiology 2022Quote: ... mouse α-σ3 (10C1 or 10G10) directly labelled with AlexaFluor 647 using the Apex AlexFluor 647 Ab-Labelling Kit (A10475, Invitrogen) at 1:100 ...
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bioRxiv - Systems Biology 2019Quote: ... whereas the poly(A)-tailed RNA in yeast cells and mouse tissues was isolated from the total RNA using a Dynabeads mRNA Purification kit (Life Technologies) following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2019Quote: Murine bone marrow cells from the femur and tibia were depleted of lineage positive cells using the EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, StemCell Techologies, Vancouver, Canada) and cultured overnight in DMEM (Gibco) media supplemented with 15% FBS (Gibco) ...
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bioRxiv - Immunology 2020Quote: 1,000 naive GFP-expressing OT-I cells were purified from a TgTcraTcrb1100Mjb;Rag2−/−;Ubc-GFP+/+ spleen with the Dynabeads Untouched Mouse CD8 Cells Kit (ThermoFisher Scientific) and transferred i.v ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Microbiology 2022Quote: Fluorescein-labeled 300 base β-actin-Mouse was synthesized using MAXIscript T7 Transcription Kit (AM1312, Invitrogen by Fisher Scientific, Waltham, MA) and Fluorescein RNA Labeling Mix (11685619910 ...
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bioRxiv - Bioengineering 2022Quote: ... RGCs were isolated from freshly dissociated organoids by magnetic microbead sorting for Thy1.2+ cells using the Dynabeads™ FlowComp™ Mouse Pan T (CD90.2) Kit (Invitrogen, 11465D) according to the manufacturer protocol.
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bioRxiv - Neuroscience 2022Quote: ... RNA samples with RNA integrity numbers (RIN) > 8.0 were further processed with the Affymetrix WT Plus kit and hybridized to Clariom S mouse arrays (ThermoFisher, Cat# 902931) as described by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: Western blot analysis was conducted using cytoplasmic extracts from mouse brain cells made with a NE-PER nuclear and cytoplasmic extraction kit (Thermo Fisher). The mixture was centrifuged at 10,000□×□g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from HeLa cells (human) or melanocytes (mouse) melan-Ink4a (Shakya et al., 2018) was isolated using a GeneJET RNA purification kit (ThermoFisher Scientific) in the presence of β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... ribosomal RNA was depleted from the samples using the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific, Waltham, MA). All samples were sequenced on an Illumina Hi-Seq sequencer to produce 150 bp paired-end reads ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated from cultured mouse neurons treated with xcTauOs (250 nM total tau) or vehicle for 6 hours using the mirVana isolation kit (Invitrogen, AM1560). RNA integrity was evaluated by capillary electrophoresis using an Agilent Bioanalyzer ...
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bioRxiv - Molecular Biology 2023Quote: ... from 150 μl plasma (single mouse or pooled) and the TG and cholesterol content were measured using colorimetric kits (Inifinity, Thermo Scientific, TG catalog #TR22421 ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were blocked again and incubated with 2-3 drops of an anti-mouse poly-HRP-conjugated secondary antibody from the Invitrogen Tyramide SuperBoost secondary staining kit (ThermoFisher, B40912). Samples were rinsed three times with 1X PBS and the tyramide working solution conjugated to AlexaFluor 488 was added and allowed to react for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched using negative magnetic selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D).
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bioRxiv - Immunology 2023Quote: ... Initial isotyping of the antibodies in hybridoma supernatants was carried out using Pierce Rapid Antibody Isotyping Kits for mouse (Thermo Fisher). Antibodies were subsequently used as confluent supernatants for most techniques ...
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bioRxiv - Neuroscience 2023Quote: Mouse embryonic fibroblast (MEF) cell lysates were processed using the Mem-PER™ Plus Membrane Protein Extraction Kit (Thermo Fisher, 89842) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: RNA Isolation: RNA extraction and isolation of mouse podocytes was performed using the PicoPure RNA isolation Kit (Thermo Fisher Scientific, #KIT0204) according to manufacturer’s protocol (100 µL instead of 50 µL of lysis buffer was applied to extract RNA) ...
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bioRxiv - Immunology 2024Quote: ... Libraries were generated using the Ion AmpliSeq™ Transcriptome Mouse Gene Expression Core Panel and Chef-Ready Kit (Thermo Fisher Scientific), according the manufacturer’s instructions ...