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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cDNA was diluted 1:10 and 2 μl of each sample was amplified using a QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Developmental Biology 2019Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) labeling was performed using the Click-iT EdU Alexa Fluor 647 Imaging Kit (Invitrogen Cat#C10340) following manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... biological triplicates or quadruplicates of mixes of 1 µg of light-labelled and 1 µg of heavy-labelled samples were analysed on an Orbitrap Velos Pro mass spectrometer coupled to an Ultimate 3000 UHPLC system with a 50 cm Acclaim PepMap 100 or EasySpray analytical column (75 μm ID, 3 μm C18) in conjunction with a Pepmap trapping column (100 μm × 2 cm, 5 μm C18) (Thermo-Fisher Scientific). Acquisition settings were ...
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bioRxiv - Neuroscience 2019Quote: Cultured DRG neurons from wild-type and TRESK KO mice were loaded with 5 μM fura-2/AM (Invitrogen, Carlsbad, CA) for 45-60 min at 37°C in culture medium ...
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bioRxiv - Cell Biology 2019Quote: ... peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap × 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm × 50 cm ...
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bioRxiv - Immunology 2019Quote: ... mid-L3 larvae were fed on fly food containing 1 mM 5-ethynyl-2 deoxyuridine (Click-iT EdU, Thermo Fisher Scientific) for 4 hours for hemocyte staining and 3 hours for lymph gland staining at 25°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 5×107 mouse ESCs (untreated and OHT-treated) were crosslinked in 10 ml 1x PBS with 2 mM DSG (Thermo Scientific) for 45 minutes at 25°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Developmental Biology 2020Quote: ... Probe 2 (mutation; 5’ FAM – CTC CCG CCT GAT T 3’) and Platinum Quantitative PCR SuperMix-UDG w/ROX (Life Technologies). The products were amplified and analysed on an ABI StepOne PCR machine.
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bioRxiv - Microbiology 2020Quote: ... ticks were homogenised in a 2 ml reaction tube with two 5 mm steel beads and 1 ml TRIzol Reagent (Thermo Scientific) using a Tissue Lyser II (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... 5×106 cells/ml PBMC were incubated at 37°C for 18 h or 2×106 cells/ml PBMC for 5 days in RPMI 1640 GlutaMAX medium (Life Technologies/Invitrogen) supplemented with 10% FCS ...
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bioRxiv - Genetics 2020Quote: ... boiled in reducing sample buffer for 5 min and resolved on 4–12% Bis-Tris Bolt gels and transferred using an iBlot 2 (Thermo Fisher). Blots were blocked in 2.5% milk in 1% TBS-Tween before staining with antibodies (Table S5) ...
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bioRxiv - Cell Biology 2021Quote: EdU (5-ethynyl-2′-deoxyuridine) detection was performed with the Click-iT EdU Alexa Fluor 555 Imaging Kit (Thermo-Fisher Scientific). EdU was diluted in DMSO to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: Brains from 2-5 days old adult flies were dissected in PBS and briefly stored in Schneider’s insect cell medium (Life Technologies, A820) supplemented with 5% heat-inactivated FBS prior to fixation in 4% paraformaldehyde in PBS for 45 min at RT ...
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bioRxiv - Biochemistry 2021Quote: ... Dried pellets were resuspended in 43 µL of water and incubated with 5 µL of 10X TURBO DNase buffer and 2 µL of TURBO DNase (Thermo Fisher) for 1 hour at 37°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... cell pellets were resuspended in 1 mL FACS buffer (DPBS with 2% BSA, Miltenyi Biotec #130-091-376, plus 0.05mM EDTA, ThermoFisher #15575020, and 5 ng/mL SCF, ThermoFisher #PHC2115) and incubated with Zombie Violet Fixable Viability dye (BioLegend #423113 ...
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bioRxiv - Biochemistry 2021Quote: Approximately 20 micrograms of purified 6xHis-tagged KRAS4BA59T were reduced with 5 mM tris(2-carboxyethyl)phosphine (ThermoFisher Scientific, Cat #77720) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... packed with ReproSil- Saphir C18 1.5 μm (Dr. Maisch GmbH, Ammerbuch, Germany) with a 2 hours segmented gradient using an EASY-nLC 1,200 (Thermo Fisher Scientific). LC solvents were A ...
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bioRxiv - Bioengineering 2022Quote: Cell proliferation in T-SLICE zones was analyzed using an EdU (5-ethynyl-2’-deoxyuridine) Cell Proliferation Kit (Click-iT− EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 488 dye: Invitrogen). Cell culture media was supplemented throughout with 10 uM EdU ...
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bioRxiv - Cancer Biology 2022Quote: ... newly synthesized DNA was labeled using the Click-iT Plus 5-ethynyl-2’-deoxyuridine (EdU) Alexa Fluor 647 flow cytometry assay kit (ThermoFisher Scientific) according to manufacturer ‘s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Plant Biology 2022Quote: ... peptides were pre-concentrated with 0.1% TFA on a trap column (AcclaimR PepMap 100 column, 100 μm i.d. × 2 cm, C18, 5 μm particle size, 100 Å pore size; Thermo Scientific) at a flow rate of 7 μL/min for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... the media was aspirated from the cells and replaced with the Fura2-loading solution (5 µM Fura-2-AM and 0.15% Pluronic F127 (Invitrogen, Waltham, USA) dissolved in D-MEM/Ham’s F-12 with 10 %FBS) ...
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bioRxiv - Synthetic Biology 2022Quote: ... a 1:10 dilution was performed and 2 μl of diluted DNA was amplified by means of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Molecular Biology 2022Quote: ... Five microliters of sample were loaded onto the trapping column (Acclaim PepMap RSLC, 100 μm × 2 cm, nanoViper, 5 μm 100Å C 18 particles, Thermo Scientific) using 0.1% acetic acid at a flow rate of 7000 nl/min for 3 minutes at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed with 2× saline sodium citrate (SSC) buffer for 5⍰min and blocked with UltraPure Salmon Sperm DNA (Thermo Fisher) in QuikHyb Hybridization Solution (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... A 3% agarose gel with 4 μL ethidium bromide was loaded with 5 μL PCR reaction and 2 μL GeneRuler 100bp ladder Plus (Thermo Fisher) and run for 40 minutes at 100 volts ...
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bioRxiv - Microbiology 2023Quote: ... recipient animals were lightly anesthetized with isoflurane and retro-orbitally injected with 2 to 5 million live donor bone marrow cells resuspended in 50 μL of 1X PBS (Gibco, MA). Donor animals were rested for a minimum of 6 weeks before MHV-A59 infection or up to 11 weeks for assessing Ly6e expression and degree of chimerism.
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bioRxiv - Microbiology 2022Quote: ... The Hela cell line was provided by HeLa CRM-CCL-2™ and maintained at 37°C with 5% CO2 in Dulbecco’s high glucose modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... in a humidified incubator at 37 °C in a 5% CO2 atmosphere in wells containing Neurobasal medium supplemented with 2% B27 (Thermofisher #17504044), 1% N2 (Thermofisher #17502048) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 × 5 min) and incubated with secondary antibody (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) and with Alexa Fluor 633-conjugated Streptavidin (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... and 5% w/v sucrose-2% w/v agar food supplemented with 2.75% w/v yeast extract (Fisher Scientific, Waltham, MA), 2.75% w/v tryptone (VWR ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to a Orbitrap Eclipse mass spectrometer (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... the nucleus was counterstained with 0.5 µg/mL 4’6-diamidino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Prolong® Gold (Life Technologies).
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bioRxiv - Physiology 2023Quote: ... Real-time PCR was performed using RealQ Plus 2× Master Mix Green (Ampliqon, #A324402) on a QuantStudio 5 machine (Applied Biosystems). Expression was calculated using the delta-delta-Ct method ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 µm C18 material (Thermo Fisher, 164564) using 0.1% formic acid in water (solvent A) ...
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bioRxiv - Cancer Biology 2023Quote: ... Coverslips were then blocked by incubation in 2% BSA in PBS at RT for 30 min followed by 5% donor bovine serum (ThermoFisher Scientific) for 20 min ...
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bioRxiv - Plant Biology 2023Quote: ... The cDNA was then diluted 20 times and 5 µl of the dilution was mixed with 2×VeriQuest Fast SYBR Green qPCR Master Mix (Affymetrix, 75690) and primers for the real-time qPCR analysis ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were loaded on pre-column (C18, 5 µm, 100 A°, 100 µ, 2 cm Nano-viper column # 164564, Thermo Scientific) at 5 µl/min flow rate for about 10 minutes and then peptides were separated on a 50 cm column (C18 ...
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bioRxiv - Immunology 2023Quote: ... 5 μm particle size) and an analytical column (PepMap C18, 500 x 0.075 μm, 2 μm particle size, Thermo Fisher Scientific), coupled to an Orbitrap Eclipse mass spectrometer (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Samples were then resuspended in 300 μl PBS + 2% FBS together with 5 μl CountBright™ Absolute Counting Beads (ThermoFisher Scientific) and run on a 5-laser BD Fortessa (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Biochemistry 2023Quote: ... The temperature was increased from 5 °C to 95 °C over 2 hours and readings taken using a One Step Plus RT-PCR (Applied Biosystems).
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bioRxiv - Plant Biology 2023Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap, C18 5 µm, 100 µm x 2 cm, Thermo Scientific) connected to an analytical column (Acclaim PepMap 100 ...
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bioRxiv - Cell Biology 2023Quote: ... From day 0 to day 5 the medium was changed daily with 2 mL DMEM/F-12 containing 20% KnockOut™ Serum (ThermoFisher), 1 x Non Essential Amino Acid (NEAA ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...