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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... dUTP was added to DNA ends by pelleting stored cells and resuspending in 100 μL of elongation buffer (1X terminal deoxytransferase [TdT] buffer, 2.5 mM CoCl2, 0.3 mM 5-bromo-2’-deoxyuridine [BrdU] [Invitrogen, Waltham, MA]), then adding 5 U of TdT (Thermo-Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... FACS buffer (5% bovine serum albumin [BSA], 2 mM EDTA, and FluoroBrite Dulbecco’s modified Eagle’s medium [Thermo Fisher Scientific, A1896701]) was used for diluting the antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Biochemistry 2023Quote: ... and human phosphatidylinositol 5-phosphate 4-kinase type-2 beta (hPIP4KB; Uniprot #P78356) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). Gene sequences were subcloned into either bacterial ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Neuroscience 2023Quote: ... or DIV12 (overexpression) primary hippocampal neurons were transfected with 1-2 μg plasmid DNA and 5 μL Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µm particle size) and an analytical column (PepMap C18, 500 x 0.075 mm, 2 µm particle size (Thermo Scientific), coupled to a Q Exactive HF-X Orbitrap (with HCD ...
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bioRxiv - Developmental Biology 2023Quote: Aiptasia or Nematostella polyps were relaxed for 15 min in 0.1M MgCl2 in sea water or Nematostella medium and transferred to sea water or Nematostella medium containing 300 µM EdU (5-ethynyl-2′-deoxyuridine, Invitrogen) 2%DMSO 0.1M MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
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bioRxiv - Genomics 2023Quote: The PPMI iPSC lines were thawed and grown on matrigel (Corning)-coated plates with Essential 8 Flex (E8, Batches 1, 2 and 3) or Essential 6 (E6, Batches 4 and 5) media (both Gibco) for about one month (5 passages) ...
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bioRxiv - Cancer Biology 2023Quote: The sonicated samples (approximately 5 µg chromatin/sample) were cleared for 2 hours using non-coupled magnetic beads (Protein G Dynabeads, Invitrogen), then further mixed with 5 µL antibody (anti-H3K27me3 ...
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bioRxiv - Developmental Biology 2023Quote: Timed pregnant females were injected intraperitoneally with 13 μg per g body weight of 5-ethynyl-2’-deoxyuridine (Edu; Invitrogen) and sacrificed after 1 hour or 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and an Acclaim PepMap 100 C18 HPLC nanoViper Trap Column (100Å, 100 μm X 2 cm, 5 μm; ThermoFisher, USA). The tryptic peptides were separated by increasing concentrations of 80% acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Genomics 2023Quote: ... For the construction of the RNH2A KO clones, RNASEH2A (Chr19, exon 2) gRNA (5’-TAACAGATGGCGTAGACCAT-3’) was cloned into GeneArtTM CRISPR Nuclease Vector with OFP reporter (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Physiology 2023Quote: ... was washed with wash buffer II (150mM NaCl, 50mM Tris pH 7.5, 5% glycerol) on the DynaMag-2 magnet (ThermoFisher Scientific) and added to each lysate and rotated for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... TEV (2): 50 µg H*-Cdc42:CTIS was mixed with 5 or 50 U of AcTEVTM protease (10 U/µL) (Invitrogen) in SEC buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile in 0.1% formic acid and loaded at 5 μL/minute onto a PepMap C18 trap column (Thermo Scientific; 5 μm 100 Å particle size ...
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bioRxiv - Molecular Biology 2023Quote: ... cells at exponential growth phase were incubated with 200 μM of the thymidine analog EdU (5-ethynyl-2′-deoxyuridine - Invitrogen) at 26 °C for 10.2 h (estimated doubling time of L ...
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bioRxiv - Microbiology 2023Quote: ... 4×105 spores and 200µl of a bacterial suspension were spread on SM/5 agar plates ( 2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 5 min) on a rocking platform and stained with 4′,6-diamidino-2- phenylindole dihydrochloride (DAPI) (Life Technologies) staining (2 μM ...
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bioRxiv - Cell Biology 2023Quote: ... We removed the supernatant and gently resuspended cells with 2 ml 1X PBS containing 5 μl SUPERaseIn RNase Inhibitor (Invitrogen) and 2.5 μl RiboLock RNase Inhibitor (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: Protein (2 µg total lysate, 5 µg soluble lysate) was loaded onto 4-12% Bis-Tris NuPAGE minigels (Invitrogen, NP0322BOX). 10 µL of precision plus protein kaleidoscope prestained standard (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... and an Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) were used to separate tryptic peptides by increasing concentrations of 80% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
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bioRxiv - Genetics 2023Quote: ... 2–5 μg of total RNA was reverse transcribed using a Superscript First-Strand Synthesis system (Invitrogen, Carlsbad, CA, USA). The quantitative RT-PCR was performed using the suh1-4 RT primers (Supplementary Table S2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were incubated in a 5% CO2 environment at 38.5°C in 2 mL RPMI 1640 media (Gibco, UK) with 10% FBS (Gibco One-Shot FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Synthetic Biology 2024Quote: ... the full volume of media in each well was pipetted gently 4-5 times and added to 1 mL of FACS buffer containing 3 uM DAPI (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA, 3 uM DAPI (Thermo Scientific #62247)) ...
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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bioRxiv - Bioengineering 2024Quote: ... and splenocyte to resuspend post centrifugation for 5 minutes and subsequently diluted with 5mL ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS ...
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bioRxiv - Immunology 2024Quote: ... were grown as a monolayer at 37°C with 5% CO2 in RPMI 1640 supplemented with 2 mM L-glutamine (31870-025, Thermofisher), 1 mM sodium pyruvate (11360-039 ...
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bioRxiv - Microbiology 2023Quote: ... Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Fisher) solution ...
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bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, 164199, ThermoFisher) and separated on an in-house packed analytical column i.d ...
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bioRxiv - Bioengineering 2021Quote: ... and 200 μL of 0.5 M pH 8.0 EDTA (ThermoFisher, 15575020) into DPBS (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was prepared using M-MLV Reverse Transcriptase (Thermo Fisher Scientific) with random hexamers (Sigma-Aldrich-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... and captured sequences were isolated using M-270 Streptavidin Dynabeads (Invitrogen). PCR amplification was performed for 12 cycles ...
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bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
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bioRxiv - Biophysics 2021Quote: ... then was bound to Dynabeads M-280 Streptavidin beads (Life Technologies). Bound DNA molecules were subjected to end repair ...
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bioRxiv - Neuroscience 2022Quote: ... a 10×10 cm square of Parafilm M (Fisher Scientific, S37440) was first rubbed on both sides against a human skin surface free of cosmetics ...
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bioRxiv - Cell Biology 2022Quote: ... M-MLV reverse transcriptase and RNase OUT (Invitrogen, Thermo Fisher Scientific) to generate cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... M-MLV reverse transcriptase and RNase OUT (Invitrogen, Thermo Fisher Scientific) to generate cDNA ...