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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Reactions were diluted 2–5 fold with H2O and qPCR was performed using PowerUp SYBR Green PCR Master mix (Thermo Fisher Scientific), 2 μL diluted cDNA preparation ...
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bioRxiv - Neuroscience 2023Quote: ... the fish were paralyzed before being mounted in agarose by bathing them for 2-5 min in a solution of 1 mg/mL α-bungarotoxin (Thermofisher Scientific) in E3 medium ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 1 ng of coding plasmid diluted in 1.5 μg of pCEP4-△CMV carrier plasmid (77) per mL of culture at 2 ξ 106 cells/mL using Expifectamine (ThermoFisher Scientific). The medium was replaced 2 h post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: Human/PDX/PBMC cells were resuspended in PBS+2% FBS at a final concentration of 10×106 cells/mL in 5 mL FACS tubes after filtering (Fisher Scientific #352235). Cells were blocked with mouse IgG (Sigma #I5381 ...
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bioRxiv - Cell Biology 2023Quote: ... intestinal fragments were incubated in epithelial cell solution (10 mM EDTA [Invitrogen, #15575020]-HBSS, 5 mM HEPES [Invitrogen, #15630080], 100 U/ml Pen-Strep [Invitrogen, #15140122], and 2% FBS [Life Technologies, #26140079]) for 30 min on ice with continuous gentle shaking ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Plates were measured at A470 and 25 °C every 5 min for 2 h using a Varioskan Flash multimode reader (Thermo Scientific, USA). For dose-dependent assays ...
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bioRxiv - Cell Biology 2023Quote: ... the cells (or fibers) were washed with PBS and counterstained with 4’6- diamidino-2-phenylindole (DAPI, 5 mg/ml, Molecular Probes, Invitrogen, Carlsbad, USA) in PBS to label nuclei.
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed 3×5 min in PBS and incubated for 2 h with an Alexa Fluor® Plus 555-conjugated secondary antibody (Invitrogen A32816) diluted in the blocking solution ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated for 5 min with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Dojindo, D523) and then mounted with PermaFluor (Thermo Fisher Scientific). Images were acquired using a BC43 or LSM800 instrument equipped with a Zeiss Axio Observer Z1 and a LSM 800 confocal unit with Airyscan module ...
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bioRxiv - Immunology 2023Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Neuroscience 2023Quote: ... and the cultures were incubated at 37°C with 5% CO2 for 2 hours before the culture medium was replaced with growth medium (Neurobasal A (Thermo Fisher, 12349015) supplemented with 1 x B27 Plus (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Whole brains were extracted from euthanized mice (5% isoflurane exposure followed by cervical dislocation) and incubated for 5 minutes on ice in cellular stabilizing solution: 45% HEBG medium (2% 50X B27 (Thermo Fisher; 17504044) and 0.25% 100X Glutamax (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: To analyze proliferation in vitro, B cells (2 x 106, purified as above) were labeled with 5 µM CellTrace Violet (CTV) (Invitrogen, Waltham, MA) and then activated and cultured as above ...
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Activation of glucocorticoid receptor signaling inhibits KSHV-induced inflammation and tumorigenesisbioRxiv - Cancer Biology 2023Quote: ... Cell cycle was examined by flow cytometry following 5′-bromo-2′-deoxyuridine (BrdU) labeling (BrdU: Sigma-Aldrich, MB5002; BrdU antibody: Invitrogen, B35129) and propidium iodide (PI ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 µm x 2 cm, particle size 5 µm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (35 cm x 75 µm ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatographic separation was carried out using an Acclaim Pepmap RSLC trap column (100 µm × 2 cm, 5 µm particles, 100 Å pores, C18, Thermo Fisher), and nanoEase M/Z Peptide BEH analytical column ...
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bioRxiv - Developmental Biology 2023Quote: ... three T175 flasks of Ishikawa cells at 60-70% confluence were supplemented with 1 µM 5-ethynyl-2’-deoxyuridine (EdU, Thermo Fisher Scientific) and incubated overnight to allow EdU incorporation into the DNA ...
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bioRxiv - Cell Biology 2024Quote: Sor-treated myotubes on 18 mm laminin-coated coverslips were loaded with 5 µM Ca2+ indicator FURA-2 AM (#F1221, Thermo Scientific, US) along with 2.5 mM Probenecid (#P36400 ...
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bioRxiv - Biochemistry 2023Quote: ... 6 μL was injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 μm C18 material (Thermo Fisher, 164564) using 0.1% formic acid in water (solvent A) ...
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bioRxiv - Cancer Biology 2023Quote: LN-229 cells were seeded at 2×10^5 cells/well into in 6-well Nunc™ Cell-Culture Treated Multidishes (ThermoFisher, #140675) and incubated overnight in reduced serum media at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 μg aliquot of each sample were loaded at 15 μL/minute onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293FT and HEK293FT-LP cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco 25300-054). All HEK293FT cell lines generated by engineering either the HEK293FT or HEK293FT-LP parent cell lines were cultured in the same way ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (35 cm x 75 μm ...
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bioRxiv - Immunology 2024Quote: ... 2 μg of each sample were loaded at 15 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
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bioRxiv - Molecular Biology 2023Quote: ... Then 3 µl of sample at 0.07 mg/ml concentration were applied to the glow-discharged grids and blotted for 2 s with blotting force -5 using a FEI Vitrobot (Thermo Fisher Scientific) at a temperature 10 °C and 100% humidity ...
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bioRxiv - Neuroscience 2024Quote: ... Digests were loaded onto a trapping guard column (EASY-column, 2 cm long, ID 100 μm, packed with Reprosil C18, 5 μm particle size from Proxeon, Thermo Fisher Scientific) at a maximum pressure of 160 Bar ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription (RT-PCR) was performed on 2-5 μg of RNA using the SuperScript III First-Strand Synthesis System (ThermoFisher Scientific, 18080051). Real time qPCR was conducted in a 384-well PCR microplate (Fisher scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 12 alternating fractions were resuspended in 2% formic acid/5% acetonitrile and subsequently injected for analysis on an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific). For the yeast cell PISA ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... ITS (5 μg/ml insulin, 5 μg/ml transferrin, and 5 ng/ml sodium selenite, Gibco), and antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin) ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 0.1 M NaCl and 1% n-Dodecyl β-D-maltoside (DDM, Anatrace, Affymetrix) and stirred for 18 hours for solubilization ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was performed with random hexamers and M-MLV Reverse Transcriptase (Invitrogen). Real-time PCR was performed in triplicates with LC FastStart DNA Master SYBR Green I on a LightCycler rapid thermal cycler system (Roche Diagnostics ...
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bioRxiv - Cell Biology 2020Quote: ... 1µg RNA was reverse-transcribed using M-MLV reverse transcriptase (Invitrogen, 28025-021). Quantitative PCR was performed with TaqMan fast Mastermix (435204 ...
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bioRxiv - Cell Biology 2020Quote: ... and was reverse transcribed using random hexamers and M-MLV Reverse Transcriptase (Invitrogen). SYBR green-based quantitative RT-PCR was performed using the Applied Biosystems 7900 Real Time PCR System ...
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bioRxiv - Genetics 2021Quote: ... and reverse transcribed into cDNAs using the M-MLV Reverse Transcriptase kit (ThermoFisher). cDNA from wild-type embryos were subjected to allele-specific PCR to eliminate heterozygotes ...
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bioRxiv - Plant Biology 2022Quote: ... RNA was reverse transcribed using M-MLV reverse transcriptase (Cat No.: 28025013, Thermofisher) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Molecular Biology 2020Quote: ... rRNA bound oligos were separated by Streptavidin conjugated magnetic Dynabeads M-280 (Invitrogen) followed by ethanol precipitation of rRNA depleted total RNAs ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse-transcription was then performed by adding M-MLV reverse-transcriptase (Fermentas/ThermoFisher) and RNase Inhibitor (Fermentas/ThermoFisher) ...
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bioRxiv - Cell Biology 2019Quote: ... Human M-CSF and Ficoll histopaque were purchased from Invitrogen (Carlsbad, CA, USA). Human CD14 magnetic beads were obtained from Miltenyi Biotec (Auburn ...
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bioRxiv - Genetics 2019Quote: ... cDNA synthesis was performed using M-MLV reverse transcriptase and random hexamers (Invitrogen). For each sample ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed directly in M-PER Mammalian Protein Extraction Reagent (ThermoFisher Scientific), separated on a NuPAGE 4-12% Tris-Glycine Gel (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... and was reverse transcribed using random hexamer and M-MLV Reverse Transcriptase (Invitrogen). SYBR green-based quantitative RT-PCR was performed using the BIO-RAD CFX96™Real-Time System ...
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bioRxiv - Biochemistry 2019Quote: ... made up to a final concentration of 1 M NaCl (Fisher Scientific, UK), pH 8.0 and incubated at 60°C for 24 hours ...