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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap, C18 5 µm, 100 µm x 2 cm, Thermo Scientific) connected to an analytical column (Acclaim PepMap 100 ...
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bioRxiv - Cell Biology 2023Quote: ... From day 0 to day 5 the medium was changed daily with 2 mL DMEM/F-12 containing 20% KnockOut™ Serum (ThermoFisher), 1 x Non Essential Amino Acid (NEAA ...
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bioRxiv - Cell Biology 2023Quote: ... Cells obtained from fractions 2–5 were combined according to the genotype and expanded in α-MEM containing 10% FBS (Gibco), 100 U/ml penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... Two larvae at a time were cold anesthetized on ice in a custom recording chamber for 5 minutes and immobilized dorsal side up in 2% low melting point agarose (BP1260-100, Fisher Scientific). Recording chambers were placed on the stage of an upright microscope (Olympus BX-51W ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed in PBS (2 x 5 min) and incubated in 0.1% Tween in PBS containing streptavidin Alexa 594 conjugate (ThermoFisher, 1:1500) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were desalted on-line using a capillary trap column (Acclaim Pepmap100, 100 μm, 75 μm × 2 cm, C18, 5 μm (Thermo Scientific)) and then separated using a 180 min RP gradient (4–30% acetonitrile/0.1% formic acid ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: Proliferative activity of 2D hydrogel cell cultures was measured using an EdU (5-ethynyl-2’-deoxyuridine) labeling kit (Click-iT™ EdU Cell Proliferation Kit for Imaging, Invitrogen). 5 μM EdU solution was dosed to cells 12 h prior to fixing with 4% paraformaldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... SCC25-Luc-GFP cells co-cultured with mouse or naked mole-rat fibroblasts were incubated with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher) for 1.5 hours ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Amoebae were grown from frozen spores at room temperature on 100 x 15mm plates filled with 30mL SM/5 agar (2 g glucose (Fisher Scientific), 2 g Bacto Peptone (Oxoid) ...
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bioRxiv - Microbiology 2023Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were placed in a digestion solution containing HBSS-glucose and 5 mM CaCl2 (Cat. #: 1564A930, Fischer Scientific) and a combination of collagenase II (2 mg/ml, Cat. #: 17101-015, Life Technologies) and dispase (5 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Pathology 2024Quote: ... The samples were loaded onto in Acclaim PepMap 100 trap column (100 μm x 2 cm, nanoViper, C18, 5 μm, 100Å; Thermo Scientific) and separated on an Acclaim PepMap RSLC (75 μm x 50 cm ...
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bioRxiv - Systems Biology 2024Quote: ... comprising a Acclaim PepMap 100 trapping column (2 cm x 100 µm i.d., 5 µm 100 Å C18, Thermo Fisher Scientific) and a consecutive nanoEase M/Z Peptide BEH analytical column (25 cm x 75 µm i.d. ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... Nuclei were labelled with a PBS-Hoechst solution at 2 μg/mL for 5 min at RT and samples were covered in mounting medium (ProLong P36930, Thermofisher scientific). Labelled cells were analysed with an inverted LSM 980 confocal microscope with Airyscan 8Y (Carl Zeiss Ltd.) ...
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bioRxiv - Pathology 2024Quote: ... 2 µl drops of yeast suspension were plated in five 1:5 serial dilutions on 100 mm square plates (Thermo Fisher) with selective SD medium and increasing concentrations (0.07–7 µg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Cell Biology 2024Quote: ... Two sets of 12 100 µL 2.66 ng/ µL enriched resuspended mitochondria aliquots in 200 µL PCR tubes were made per condition and heated on a temperature gradient of 42-64°C (2°C steps) for 5 minutes at each temperature using a Veriti 96-well thermal cycler (Applied Biosystems).
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bioRxiv - Cell Biology 2024Quote: All experiments were carried out in HeLa cells ATCC CCL-2 (ECACC General Collection) grown in a humidified incubator at 37°C with 5% CO2 in DMEM (Gibco-ThermoFisher) supplemented with 10% fetal bovine serum (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was co-transcriptionally capped using m7(3’OMeG)(5’)ppp(5’)(2’OMeA)pG capping reagent (Hongene Biotech) in a “one-pot” reaction followed by digestion with DNase I (Thermo Fisher). Linear mRNA was purified using Dynabeads MyOne Carboxylic Acid beads (Thermo Fisher).
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bioRxiv - Cancer Biology 2024Quote: ... Recipient cells were incubated with either PKH67 dye-labelled or fluorescent-labelled sEVs for 2-3 days in media supplemented with 5% exosome-depleted FBS (Gibco, USA). Cells were imaged with time-lapse microscopy or washed with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, ThermoFisher) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Immunology 2024Quote: ... The cDNA generated was then diluted 2-5 fold in RNase- and DNase-free water and mixed with PowerUP SYBR Green Master Mix (Applied Biosystems) and primers (listed in Supplemental Table 3 ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER rolls and unrolled control samples were incubated at 37 °C in complete organoid media with 50 μM of 5-Iodo-2’deoxyuridine (IdU, Fisher Scientific) and 20 uM of Telox64 (courtesy of Mark Nitz ...
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bioRxiv - Biophysics 2024Quote: ... Peptides reconstituted in 0.1% formic acid were loaded onto a trap column (Acclaim C18 PepMap 100 nano Trap, 2 cm × 100 μm I.D., 5-μm particle size and 300-Å pore size; Thermo-Fisher Scientific) at 15 μL/min for 3 min before switching the precolumn in line with the analytical column (Acclaim C18 PepMap RSLC nanocolumn ...
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bioRxiv - Microbiology 2021Quote: ... 5% human serum and 5% AlbuMAX-II (Gibco)) was replaced daily until reaching maturity at day 14-post induction ...
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bioRxiv - Immunology 2023Quote: ... 2mg of 5-ethynyl uridine (5-EU, Thermofisher) was injected intraperitoneally at D12 following SRBC immunisation and similar preparation and labelling steps described for the ex vivo 5-EU assay were followed ...
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bioRxiv - Developmental Biology 2021Quote: ... treatment and binding to Dynabeads M-280 Streptavidin beads (Thermo Fisher Scientific), second-strand synthesis was performed with Klenow Exo-enzyme (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse-transcribed into cDNA with M-MLV Reverse Transcriptase (Invitrogen, 28025013). Quantitative real-time PCR was performed on CFX384 Touch System (Bio Rad) ...
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bioRxiv - Immunology 2021Quote: ... RNA was reverse transcribed into cDNA using M-MLV Reverse transcriptase (Invitrogen). RT-qPCR was performed using gene-targeted primers (Supplementary Table 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were precipitated by adding 1:1 LiCl (7.5 M) (Invitrogen AM9480) and incubating at −20°C for 30 minutes followed by centrifugation at full speed at 4°C for 15 minutes and extensive EtOH washes ...
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bioRxiv - Neuroscience 2019Quote: ... and 400 U M-MLV reverse transcriptase (ThermoFisher Scientific; Cat. No. 28025013). Real-time PCR experiments were performed using a QuantStudio 3 Real Time PCR system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and 200 U/μl M-MLV reverse transcriptase (Invitrogen, catalogue number 28025013) at 25°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 µL each of 0.1 M DTT (Invitrogen, Cat No. 1907572), RNaseOUT™ (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... an aliquot of 100 μL carboxylic acid paramagnetic Dynabeads M-270 (Invitrogen) and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... Dynabeads M-280 streptavidin magnetic beads (Thermo Fisher Scientific, Waltham MA, USA) were used to physically isolate the RAD-tagged DNA fragments ...
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bioRxiv - Genomics 2021Quote: ... 1 M Power SYBR Green PCR Master Mix (Applied Biosystems, CA, USA), and 10 M forward and reverse primers (Supplemental Table 12) ...
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bioRxiv - Systems Biology 2021Quote: ... cell pellets were initially lysed in M-PER solution (Thermo Fisher Scientific) and samples were separated by SDS-PAGE (Criterion TGX Precast gels ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 ng was used for reverse transcription with Revertaid M-MLV (Invitrogen) with random primer decamers ...
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bioRxiv - Bioengineering 2020Quote: ... cells were collected in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific) and hydrolyzed in 6M HCl for 24 hours at 110°C ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μL of the magnetic beads (Dynabeads™ M-280 Streptavidin; Invitrogen) (10 μg ...
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bioRxiv - Neuroscience 2020Quote: ... Extracted RNA was reverse-transcribed using M-MLV reverse transcriptase (Invitrogen™). Messenger RNA (mRNA ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 M sodium acetate pH 5.5 and 20 μg of glycogen (ThermoFisher). Removal of residual DNA was verified by PCR using fimA oligos ...
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bioRxiv - Pathology 2021Quote: ... cell lysate was incubated with Dynabeads® M-270 Epoxy (Life technologies) for 1 hour at 4°C under gentle rotation ...