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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Akata cells were grown at 37°C under 5% CO2 in RPMI-1640 supplemented with 10% FCS and 2 mM of L-Glutamine (Gibco, 25030-081). Cells KD for BHRF1 were generated from Akata cells (see below ...
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bioRxiv - Systems Biology 2021Quote: ... Peptide sample was loaded onto the pre-analytical column (100 µm x 2 cm, nanoViper C18, 5 µm, 100A; Thermo Fisher Scientific) at a flow rate of 5 µl/min ...
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bioRxiv - Microbiology 2020Quote: ... One-hundred microliters of 2 ×104 to 5 ×104 conidia/ml were added to 100 ml of RPMI 1640 liquid medium (Thermo Sci Gibco, California, USA) in microtiter plates with final concentrations of antifungals ranging from 0.015 to 16 μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Fresh tissue samples were collected within 2 h of surgery and were sliced to 5 mm3 and immersed in 10 vol of RNALater (Ambion, Austin, TX). The tissue samples were stored at –80 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Sample were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2020Quote: ... cells or tissue explants were incubated with virus (MOI 0.05-5) for 1-2 hr or overnight respectively at 37°C using fresh media supplemented with fetal bovine serum (Thermo Fisher Scientific). For co-infection assays ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed with BSA-free KRB buffer with no glucose and loaded with 5 µM Fura-2 (Invitrogen, Amarillo, TX) and 0.04% Pluronic F-127 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: TSA assays were performed by mixing 5 μL protein solution at 2 mg/mL with 5 μL SYPRO®Orange (1:50 diluted from 5000x stock solution, Invitrogen, USA), 5 μl of 8-fold buffer (1200 mM NaCl and 400 mM Tris pH 8) ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were kept at 37°C and 5% CO2 in neurobasal medium containing 2 mM L-glutamine and B27 supplement (Thermo Fisher Scientific) with an exchange of half the medium after 4 days in culture.
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bioRxiv - Immunology 2022Quote: ... 1.5 μg aliquot of each sample was loaded at 15 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were maintained at 37°C in a 5% CO2 humidified atmosphere and were passaged every 2 days by dissociation with TrypLE (Thermo Fisher Scientific). Culture media was replaced with fresh stocks daily ...
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bioRxiv - Bioengineering 2020Quote: The rADSCs of the 4th passage were seeded on a six-well culture plate at a density of 5 ×105 cell/ml with 1 ml of neurosphere medium containing serum-free DMEM/F12 with 2 % B27 supplement (Thermo Fisher, USA) and 20 ng/ml of basic fibroblast growth factor (bFGF ...
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Plant Biology 2020Quote: Young seedlings were incubated in 20 μM 5-ethynyl-2’-deoxyuridine (EdU) (Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit, ThermoFisher Scientific/Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... 5×106 cells/ml PBMC were incubated at 37°C for 18 h or 2×106 cells/ml PBMC for 5 days in RPMI 1640 GlutaMAX medium (Life Technologies/Invitrogen) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2020Quote: Mice were injected intra-peritoneally at P4 and P5 with 25ug/gm weight of 5-Ethynyl-2-deoxyuridine (EdU) (Thermo Fisher Scientific) and chased for times specified (Vin WT P-21 and P-53 and Vin KO P-20 and P-36) ...
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bioRxiv - Microbiology 2019Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100 Å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 μm × 50 cm ...
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bioRxiv - Bioengineering 2020Quote: ... 30 and 60 min after induction of neutralization of Col 1 and enzymatic gelation (n=2-5 samples/group) of freshly prepared materials with a Multiskan™ GO Microplate Spectrometer (Thermo Scientific). The following solutions were analyzed ...
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bioRxiv - Genetics 2020Quote: Proteins were extracted form 20 μL of ammonium bicarbonate resuspended fractions by adding 5 μL of lysis buffer (10% NP40, 2%SDS in PBS) and quantified by BCA protein assay kit (ThermoFisher Scientific, 23225).
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bioRxiv - Biochemistry 2021Quote: ... 100 ng of the sample were loaded onto a C18 precolumn (100-µm × 2-mm Acclaim PepMap100, 5 µM, Thermo Fisher Scientific) with 2.5 % ACN/0.1 % TFA (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm × 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Immunology 2019Quote: ... In some groups culture media was supplemented with recombinant osteopontin (2 μg/ml) (R&D) or anti-CD44 (5 μg/ml) (Thermofisher; clone IM7). Cells were cultured in 5% CO2 at 37°C ...
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bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
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bioRxiv - Microbiology 2020Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 µm × 2 cm, nanoViper, C18, 5 µm, 100 Å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 µm × 50 cm ...
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bioRxiv - Immunology 2020Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100 Å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at 5×106 cells/mL in 1 mM MgCl2/PBS and treated with 2 mM DSG cross-linker (Thermo Fisher Scientific) at room temperature for 30 min on a rotator ...
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bioRxiv - Neuroscience 2022Quote: ... except that they also received 2 injections of 10 mg/kg 5-ethynyl-2’-deoxyuridine (EdU) from the Click-iT EdU Alexa Fluor 647 imaging kit (Invitrogen, Carlsbad, CA), the day after DOX injection (4 DPN ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells (CCL-2; ATCC) were cultured at 37°C and 5% CO2 in DMEM/high-glucose/GlutaMAX medium (10564029; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... 2 μL of combined 1μM forward and reverse primers (see Supplementary table 2) and 5 μL SYBR green master mix (#A25742; Applied biosystems, Waltham, USA) and run on the QuantStudio7Flex (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with 2 µl of recombinant yeast Poly(A) Polymerase (PAP) together with 5 µl of 5X PAP buffer (Thermo Fisher Scientific), 2.5 µl of 10 µM GTP/ITP/MgCl2 (Jena Bioscience) ...
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bioRxiv - Neuroscience 2022Quote: ... and the trap column used was Acclaim PepMap 100 (100 μm x 2 cm, nanoViper, C18, 5 μm, 100 Å, Thermo Scientific). Initial data analysis was performed using Proteome Discoverer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 5% Fetal Bovine Serum (FBS), 4°C) and incubated (37°C, 2 minutes) in protease solution (TrypLE express, Thermo Fisher 12604013) supplemented with DNase (100 U/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 4-5 times in PBS for 2 hours and mounted onto glass slides using ProLong Gold Antifade Mountant (Invitrogen; Cat #P36930). Sections were imaged on a Leica SP8 upright confocal laser scanning microscope using a X10/NA 0.45 objective ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Cell Biology 2022Quote: ... Immunostained cells were then washed 2 times with DPBS for 5 minutes at room temperature and mounted on glass slides using Fluoromount G (Thermo Fisher Scientific) and a coverslip ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... After the secondary antibody was washed three times for 5 min, nuclei were stained with DAPI (4’,6- Diamidino-2-Phenylindole, Dihydrochloride) (#D1306, Thermo Fisher Scientific) (dilution 1:1,000 in PBS ...
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bioRxiv - Biophysics 2023Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (75 μm x 35 cm ...
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bioRxiv - Cell Biology 2023Quote: ... Percent of cell death was determined by the staining with 5 µM Hoechst 33342 and 2 µM propidium iodide (PI) (Invitrogen, Carlsbas, CA). Images were taken and counted by Zeiss microscope equipped with automated computer-assisted software (Axiovision 4.6 ...
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bioRxiv - Cell Biology 2023Quote: ... Quantification of neuronal survival was determined by staining treated cultures with 5 µM Hoechst 33342 and 2 µM propidium iodide (PI) (Invitrogen, Carlsbad, CA) 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were trapped on an Acclaim Pepmap 100 C18 trap column (100 μm x 2 cm, particle size 5 μm, Thermo Fisher Scientific) and separated on an in-house packed analytical column (75 μm x 35 cm ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides from each fraction were resuspended in 50 µl 1% formic acid and aliquots of 4 μL were loaded at 10 μL/min onto a trap column (100 μm × 2 cm, PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
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bioRxiv - Developmental Biology 2023Quote: COV434 cells were transfected with the plasmid cloned with the gRNA directed toward the 5’ region of exon 2 of the NR2F2 gene using Lipofectamine 3000 (Thermo Fisher Scientific). Blue fluorescence protein (BFP)-positive cells were observed and photographed using a fluorescence microscope (Zeiss ...
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bioRxiv - Biochemistry 2023Quote: ... and Caco-2 cells engineered to express ACE2 88 were cultured at 37°C in 5% CO2 in DMEM containing GlutaMAX (Gibco, Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... 300 μl of the induced samples were centrifuged at 8,000xg for 2 minutes at 25 °C and resuspended in 5 μl of Dulbecco’s phosphate-buffered saline (DPBS) (Thermo Fisher Scientific 14200075), supplemented with 1 mg/ml membrane stain FM1-43 for bacteria and FM4-64 for yeast (Thermo Fisher Scientific T35356 and T13320 ...