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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... An automatic cell counter (Countess II; Thermofisher) was employed to analyze cell counts at indicated time points.
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bioRxiv - Cell Biology 2024Quote: ... 2 mg/ml AlbuMax-II (Gibco, 11021045), Small molecules ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-Strep-tag II (NC9261069, Thermo Fisher), anti-FLAG epitope tag (L00018 ...
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bioRxiv - Biochemistry 2023Quote: ... using the SuperScript II reverse transcriptase (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Taqman Universal Mastermix II with UNG (ThermoFisher), NEBNext Small RNA Library Prep kit (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... and dispase II (1.1 U/ml, Gibco) for 40 minutes at 37℃ with mild agitation ...
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bioRxiv - Cell Biology 2020Quote: ... thermoblocks at 90°C and 50°C (e.g., Thermo Fisher, 88870004), an ultracentrifuge capable of spinning 50 ml falcon tubes at 10,000 rpm (Beckman Coulter Avanti J-30 I ultracentrifuge and a Beckman JA-12 fixed angle rotor) ...
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bioRxiv - Neuroscience 2024Quote: ... Fast SYBR™C Green Master Mix (Applied Biosystems™C) was employed along with primers at a concentration of 5nM ...
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bioRxiv - Cell Biology 2020Quote: ... The Flp-In T-Rex HEK293 cells were transfected with the N-terminal GFP-tagged FAM83F packaged in a pcDNA5-FRT/TO vector along with Flp recombinase pOG44 (Invitrogen) in a ratio of 1 μg ...
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bioRxiv - Genetics 2021Quote: ... https://www.lifetein.com) polyclonal antisera raised against the N-terminal peptide of CenH3 [66] and anti-Rabbit-Alexa Fluor 488 (ThermoFisher Scientific). KNL2 and CENP-C were detected in root nuclei as previously described [67] using custom made anti-AtKNL2 [37] or anti-AtCENP-C antibodies (http://www.eurogentec.com/ ...
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bioRxiv - Neuroscience 2022Quote: Genes encoding the full-length sequences of Human PP2A A subunit and PP2A C subunit with N-terminal His tag and non-cleavable HA tag were sub-cloned into the pFastBac-Dual vector (Invitrogen). Sequences of PP2A A and C subunits are shown in Table 1 ...
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bioRxiv - Plant Biology 2022Quote: ... AtSUMO1 was cloned into destination vector pK7WGR2 (N-terminal mRFP tag, (Karimi et al., 2002)) by LR reactions using Gateway technology (Invitrogen). Vector specific primer p35s-F used in plasmid construction are listed in Table S2.
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bioRxiv - Biochemistry 2019Quote: ... pET151/D-TOPO plasmids with the sequences of interest inserted under control of Lac operon and T7 viral promoter with N-terminal hexa-histidine tag and Ampicillin selection marker was obtained from Invitrogen. Sequences were optimized for expression in E ...
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bioRxiv - Biophysics 2020Quote: A full-length cDNA encoding the methyltransferase SUV39H1 was amplified by PCR and fused with an N-terminal His6-MBP tag in pET-Duet1 (Invitrogen).
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bioRxiv - Neuroscience 2019Quote: ... a Tet-inducible EGFP-RBM45 construct was generated by inserting the EGFP-RBM45 (N-terminal EGFP fusion) into the pcDNA4/TO vector (Invitrogen). The sequences of all constructs were confirmed by Sanger DNA sequencing and the sizes of the expressed proteins confirmed by Western blot.
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bioRxiv - Evolutionary Biology 2020Quote: ... and 0.2 μg empty pQCXIP or pQCXIP-WT IFITM3 or the indicated mutant fused with amino-terminal FLAG using Lipofectamine2000 (Invitrogen). At 48 hours post-transfection ...
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bioRxiv - Neuroscience 2020Quote: ... a selected cell terminal was puffed for 5 s with a solution containing 3-5 μM FM1-43 (Molecular Probes) and (in mM) ...
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bioRxiv - Systems Biology 2020Quote: ... the mouse mNCK2 cDNA sequence was cloned via restriction enzymes in frame with a N-terminal 3XFLAG tag in pcDNA3.1(-) (ThermoFisher Scientific) for western blot (WB ...
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bioRxiv - Microbiology 2021Quote: ... nucleotide sequences encoding N-terminal FLAG-tagged VACV proteins were subcloned between the BamHI and XbaI restriction sites of a pcDNA4/TO vector (Invitrogen). Alternatively ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein with an N-terminal His10 tag was expressed in Sf9 cells following the Bac-to-Bac baculovirus system (Invitrogen). Cells were collected and solubilized to extract protein in the buffer containing 25 mM MES pH 6.0 ...
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bioRxiv - Biophysics 2022Quote: The SARS-CoV-2 Omicron RBD and the N-terminal peptidase domain of ACE2 were expressed using the Bac-to-Bac baculovirus system (Invitrogen) as previously stated ...
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bioRxiv - Immunology 2022Quote: ... Gamma and Omicron NTD mutants with an N-terminal human IL-2 or Mu phosphatase signal peptide and a C-terminal polyhistidine tag followed by an AviTag were used to express soluble NTD proteins by transiently-transfecting Expi293F cells (GIBCO). After four days ...
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bioRxiv - Neuroscience 2022Quote: Projection neurons were retrograde labeled from their axon terminals by pressure injection of Alexa-conjugated Cholera Toxin B (Ctb, Invitrogen) or Fast Blue (Polysciences ...
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bioRxiv - Genetics 2022Quote: ... WT and missense variant cDNAs were transferred from pDONR223 to MSCV_IP_N terminal-HA-FLAG vector using Gateway recombination (Thermo Fisher Scientific). These vectors were used to produce retrovirus by transfection of HEK293T (ATCC ...
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bioRxiv - Biochemistry 2019Quote: HelQ and the N-terminal HelQ fragment (N-HelQ) were over expressed using the Bac-to-Bac baculovirus expression system from Gibco BRL in SF9 insect cells ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Microbiology 2020Quote: ... and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen), previously digested with the same enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... and recombined with an RPS5A::XVE promoter and an N-terminal GFP in a pB7m34GW backbone (Karimi et al., 2007) via an LR reaction (Invitrogen) and verified by restriction digest.
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bioRxiv - Biophysics 2021Quote: ... norvegicus with a HA-tag added to the C-terminal end representing the N-terminal domain (Met 1 to Val 469) of sAC (57) was cloned into pcDNA5/FRT (Invitrogen).
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bioRxiv - Microbiology 2020Quote: The infectivity of lentivirus particles carrying SARS-CoV-2 spike proteins was determined using a vector containing an HIV-1 or MLV long terminal repeat (LTR) that expresses a Gaussia luciferase reporter (Gluc)(50, 51) 293T cells were cultured in DMEM (Gibco) media ...
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bioRxiv - Biochemistry 2022Quote: The T7 gp5.9 gene from phage T7 was produced as a synthetic gene construct with an N-terminal 3C-cleavable histidine tag (GeneArt, Invitrogen). This was subcloned into the pACEBAC1 (MultiBac ...
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bioRxiv - Immunology 2022Quote: ... The Hc gene with a N-terminal gp67 signal peptide and Lc gene with a N-terminal honeybee melittin signal peptide were subcloned into pFastBac Dual vector (Invitrogen) containing two multiple cloning sites ...
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bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... dUTP was added to DNA ends by pelleting stored cells and resuspending in 100 μL of elongation buffer (1X terminal deoxytransferase [TdT] buffer, 2.5 mM CoCl2, 0.3 mM 5-bromo-2’-deoxyuridine [BrdU] [Invitrogen, Waltham, MA]), then adding 5 U of TdT (Thermo-Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products of ame1 and okp1 point mutants (with C-terminal TAF tag and G418 marker) were amplified from the mutant plasmids using a high-fidelity DNA polymerase (SuperFi, Invitrogen) and transformed into the respective haploid ame1 and okp1 null mutants ...
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bioRxiv - Neuroscience 2023Quote: Mice were given a terminal dose of pentobarbital and perfuse-fixed with PBS followed by 4% formaldehyde solution (Thermofisher 28908) in 0.1M PB pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: hiPSC-derived neurons after 38-42 days of terminal differentiation were transfected with pCAG-MaxGFP (GFP) using Lipofectamine 2000 (Invitrogen). The GFP-labeled neurons were imaged at 10x magnification using a Nikon C2 confocal fluorescence microscope ...
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bioRxiv - Plant Biology 2023Quote: ... For C-terminal fusions the CDS of PYE and OLV versions were amplified from cDNA of Fe deficient Arabidopsis WT roots with primer pairs PYE_B1 fw/PYEns_B2 rev and OLV_B1 fw/OLVns_B2 rev carrying B1 and B2 attachment sites without stop codon or from existing pDONR with N-terminal fusion (Supplemental Table S1) and transferred via Gateway cloning into pDONR207 (Invitrogen) according to the manual (Gateway ...
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bioRxiv - Cell Biology 2023Quote: ... we performed a Click-iT Plus TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay (Invitrogen C10617, Alexa Fluor 488), which detects DNA breaks formed when DNA fragmentation occurs at the end of apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: ... and patient-derived cell cultures (P022.C, P024.C, P030.C) were cultured in Dulbecco’s modified Eagle medium (DMEM, #41966, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... The protein denaturation was monitored by obtaining the fluorescence signal by increasing the temperature from 22 °C-95 °C at 0.5 °C/minute rate using QuantStudio 3 real-time PCR (ThermoFisher). The melting temperature (Tm ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: Genomic DNA was extracted from the PhaZ-producing strains (RNA/DNA purification kit, Norgen Biotek for C. testosteroni, and GeneJET Genomic DNA Purification Kit, ThermoFisher Scientific for other strains). Inserts were obtained by amplifications of the mature phaZ genes (without signal peptides ...
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bioRxiv - Microbiology 2020Quote: ... The Madin-Darby Canine Kidney II (MDCK-II) cell line was maintained in Eagle’s Minimum Essential Medium (EMEM, Gibco), supplemented with 5% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... Gateway recombination reactions were performed with BP Clonase II and LR clonase II enzyme mixes (Invitrogen, Carlsbad, CA, USA) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and stored at −80°C until DNA extraction with a magnetic-based method (MagMAX CORE extraction kit – ThermoFisher).