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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... for 2 h at 55°C and DNA was purified using DNA clean-up kit (Thermo Fisher Scientific). DNA fragmentation range was assessed by electrophoresis.
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bioRxiv - Molecular Biology 2021Quote: ... for 4 h at 55°C and DNA was purified using DNA clean-up kit (Thermo Fisher Scientific). DNA fragmentation range was assessed by electrophoresis.
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bioRxiv - Plant Biology 2020Quote: ... and afterwards digested overnight at 37 °C with trypsin (Sigma; in-gel tryptic digestion kit, Thermo Fisher Scientific), desalted with C18 Ziptips (Millipore) ...
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bioRxiv - Genetics 2020Quote: mRNA was extracted from AX2 cells stored at −80 °C using Dynabeads mRNA direct micro purification kit (Invitrogen). Samples were lysed using tissue homogenizer (Kontes ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was subjected to bisulphite-meidated C to U conversion using MethylCode Bisulfite Convrsion Kit (ThermoFisher Scientific), and then used as a template for PCR for 35 cycles with EpiTaq (TAKARA ...
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bioRxiv - Physiology 2021Quote: ... DNase treatment was performed during 30 min at 37°C following the manufacturer’s protocol (TURBO DNase, kit Invitrogen), except that RNasine 0.36 U/μL (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... and Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C-10424) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was reverse transcribed for 30 min at 50°C using the SSIII One-Step Kit (Thermo Fisher) followed by 45 PCR cycles of 94°C for 15 seconds ...
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bioRxiv - Genetics 2022Quote: ... DNA from the affected siblings in family C was exome sequenced using Ampliseq whole exome kit (ThermoFisher Scientific) and run on a Proton Sequencer ...
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bioRxiv - Genetics 2022Quote: ... Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific) or the Pierce HA Tag IP/Co-IP Kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... C-Myc) introduced by Sendai virus according to the manufacturer’s instructions (Cytotune iPS 2.0 Sendai reprogramming kit, ThermoFisher) or by using an episomal method ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and the concentration determined using a BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific). After running an SDS PAGE (12%) ...
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bioRxiv - Microbiology 2024Quote: ... fixed at 50°C for 50 minuftes and stained with LIVE/DEAD BacLight Kit (Molecular Probes-Invitrogen, USA). Biofilms were visualized by confocal laser scanning microscopy (CLSM-Leica SP8 inverse ...
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bioRxiv - Microbiology 2024Quote: ... fixed at 50°C for 50 minuftes and stained with LIVE/DEAD BacLight Kit (Molecular Probes-Invitrogen, USA). Biofilms were visualized by confocal laser scanning microscopy (CLSM-Leica SP8 inverse ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Bioengineering 2023Quote: ... The protein samples were heated from 25 °C to 95 °C at a rate of 0.3°C/sec in a StepOnePlus real-time PCR system (Applied Biosystems), and fluorescence was captured in the ROX channel ...
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bioRxiv - Cell Biology 2020Quote: ... The concentrations of IgG in cell lysates and supernatants after recycling were measured with an IgG Human ELISA Kit (88-50550, Invitrogen), following the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... The extraction and purification of human genomic DNA were performed using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific) and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... cerevisiae EKY3/pYES2-TOP1 induced for expression of wild-type human TOP1with the PrepEase Histidine-Tagged Protein Purification Midi Kit – High Specificity (from Affymetrix) according to the manufacturer’s procedures ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Physiology 2022Quote: ... human TACAN or their mutants were synthesized by in vitro transcription using the T7 mMESSAGE mMACHINE kit (Invitrogen, Waltham, MA) and injected into oocytes (25 ng each) ...
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bioRxiv - Systems Biology 2022Quote: ... RNA from human skin and wounds was reverse transcribed using TaqMan® Advanced miRNA cDNA Synthesis Kit (Thermo Fisher Scientific). Individual miRNA expression was then quantified using TaqMan® Advanced miRNA Assays (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Total or individual CD8+ or CD4+ T cells were purified from PBMC suspensions from our PLWH and HIV-1 negative control study cohorts by negative immunomagnetic selection (purity >90%) using the Untouched Human T cell and CD4 T cell Dynabeads Kits (Invitrogen) and the MojoSort Human CD8 T cell (BioLegend ...
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bioRxiv - Cancer Biology 2019Quote: ... and CD19+ B-cells were isolated by negative selection using the Dynabeads® Untouched™ Human B Cells kit (Invitrogen). Mantle cell lines (Z-138 ...
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bioRxiv - Molecular Biology 2019Quote: ... The resulting supernatant was decanted and the concentration of secreted IL6 was measured by the human IL6 ELISA kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2019Quote: ... Extracted DNA samples were quantified following the Quantifiler Duo Human DNA Quantification Kit (Thermo Fisher Scientific, Inc., Waltham, MA, USA) as recommended by the manufacturer in 7500 Real-time PCR System (Thermo Fisher Scientific Company ...
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bioRxiv - Bioengineering 2021Quote: ... IL-8 concentrations in medium samples from the basal compartment were measured using the IL-8 Human Uncoated ELISA Kit (88-8086-88, Invitrogen). Assays were performed in Nunc MaxiSorp (tm ...
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bioRxiv - Biophysics 2020Quote: Expression was conducted according to the manual of 1-Step Human High-Yield Mini in vitro translation (IVT) kit (Product No. 88891X) distributed by ThermoFisher Scientific (Pierce Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells and NK cells were isolated from cryopreserved PBMCs using commercial kits (MagniSort Human CD4+ T Cell Enrichment; Affymetrix, and MagniSort™ Human NK cell Enrichment ...
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bioRxiv - Immunology 2022Quote: ... Globin mRNA was depleted from a portion of each total RNA sample using the GLOBINclear™-Human kit (Thermo Fisher). Following the removal of globin transcripts transcriptome profiles were generated via mRNA sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit, Invitrogen). All the steps were carried out following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Medium from each condition was removed and E-cadherin concentrations were quantified by using Human E-Cadherin Quantikine ELISA kit (Invitrogen). The Human E-Cadherin EIA kit protocol was followed as described ...
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bioRxiv - Immunology 2019Quote: ... Concentrations of 30 cytokines were measured using the 30-plex magnetic human Luminex cytokine kit (all using lot ID 1805187A, ThermoFisher). Samples were measured on a LX200 (Luminex ...
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bioRxiv - Molecular Biology 2021Quote: ... Human globin mRNA was depleted from all samples except from samples #1 and #2 using the GLOBINclear kit (ThermoFisher Scientific). The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent ...
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: The VEGF-A concentration in the culture medium was analyzed using a VEGF-A Human ProcartaPlex Simplex Kit (Cat. no. EPX01A-10277-901, Invitrogen), specific for the detection of VEGF-A165 ...
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Immunology 2020Quote: ... the cytokine levels were determined by Luminex xMAP technology using Human Custom ProcartaPlex Assay kit (Thermofisher, Cat PPX-04-MXRWEYU) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and enriched for CD8+ T cells with the MagniSort™ Human CD8+ T cell Enrichment Kit (#8804-6812-74, Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from rat glomeruli tissue and human podocyte samples using the mirVanaTM Isolation Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... total cell RNA was extracted from FACS-purified LP or HP cell populations from B16-F10 murine or LM-MEL-28 human melanoma cells using PureLink RNA Mini kits (ThermoFisher), including on-column DNase treatment ...
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bioRxiv - Cell Biology 2022Quote: ... >1.5 kb 5’and 3’ homology arms were amplified from genomic DNA isolated from one 6-cm dish of immortalized human retinal pigment epithelial cells (hTERT-RPE1 cells, ATCC) using a Purelink Genomic DNA Mini Kit (ThermoFisher). The π-element was PCR amplified from plasmid #1 ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... PBMCs were isolated by density gradient using Lymphoprep (Axis-Shield) and CD4+ T cells purified by negative selection using the Dynabeads Untouched Human CD4+ T Cell Isolation kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... commercially available kits were purchased and the assays done following the manufacturer’s protocol: IL-1β (Mouse: BMS6002; Human: BMS224INST, Thermo Fisher) and HMGB1 (Mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... The pENTR-hMCAK construct was generated by amplifying the coding sequence of human MCAK by PCR and inserting it into a pENTR entry clone using the pENTRTM/D-TOPO kit (ThermoFisher). The pFB-6HF2hMCAK construct was created from pFB-6HFRET2-DEST vector and pENTR-hMCAK constructs using Gateway® cloning technology (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... These mRNAs were generated from cloned human cDNAs inserted into pCS2+ and in vitro transcribed using mMessage mMachine SP6 kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Transcriptome libraries were generated using automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The transcriptome libraries were barcoded ...