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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant baculovirus containing the sequence of ΔPRD-dynamin 1 with an N-terminal His-tag was generated by following Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). The suspension cultures of Sf9 were maintained in Sf-900 III serum-free media (SFM ...
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bioRxiv - Genomics 2020Quote: ... where 2–3 mm of the terminal ends of 3–4 filaments from the left side each fish were collected and placed in RNAlater (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The coding sequence of human PLIN2 with an N-terminal EGFP tag was provided by John McLauchlan (Targett-Adams et al., 2003) and re-cloned into pCR8 (ThermoFisher Scientific). The human PLIN3 (TIP47 ...
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bioRxiv - Bioengineering 2021Quote: ... nanobody sequences were PCR amplified with primers containing terminal regions of homology for cloning into a pRset plasmid (Addgene plasmid 3991)(Invitrogen, V35120) in frame and linked to mCherry ...
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bioRxiv - Cell Biology 2021Quote: ... The pcDNA5/FRT-PRPF4 plasmid was constructed by cloning the PRPF4 gene fused to an N-terminal FLAG/HA tag into the pcDNA5/FRT plasmid (Thermo Fisher).
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bioRxiv - Biochemistry 2022Quote: ... was cloned in frame with a N-terminal GFP tag and intervening short peptide linker (GSGEGRG) into the pcDNA5/FRT/TO bacterial expression vector (Invitrogen; V652020). A C-terminal histidine tag (Hisx6 ...
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bioRxiv - Neuroscience 2019Quote: pLVX-EF1a-TS-EGFP-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged EGFP cDNA (DNA String byGeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Neuroscience 2019Quote: ... pLVX-EF1a-TS-OPT-FUS-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged codon optimized FUS cDNA (Gene synthesis by GeneArt, Life Technologies) into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... and its N-terminal amino-acid sequence was analyzed by Edman microsequencing(39) on a Procise 494 cLC protein sequencer (Applied Biosystems), using the standard pulsed-liquid program for PVDF-blotted proteins.
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bioRxiv - Biochemistry 2023Quote: Human GRK2 S670A with a C-terminal hexahistidine tag was expressed in Spodoptera frugiperda (Sf9) cells using the Bac-to-Bac insect cell expression system (Life Technologies). The insect cells were harvested 48 hours post-infection and homogenized with buffer containing 20 mM HEPES pH 8.0 ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Genomics 2023Quote: The Myone C1 beads with the cDNA library were resuspended in 100 μl of TdT mix (1x Terminal Transferase Reaction Buffer, 0.25 mM CoCl2, 1 µM dGTP/ddGTP mix (0.95 µM dGTP (Thermo Fisher Scientific, R0161), 0.05 µM ddGTP (MilliporeSigma ...
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bioRxiv - Plant Biology 2023Quote: ... and AtILR1 open reading frame sequences deleted of the 25-N-terminal signal peptide-encoding codons were amplified using Phusion Taq Polymerase (Thermo Fisher) prior to cloning into pETM11 plasmid in the BL21 (DE3 ...
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bioRxiv - Cell Biology 2023Quote: From Expi293 cells: Constructs in pCMV10 vector with N terminal 3xFLAG tag were transfected into Expi293F cells (A14527; Thermo Fisher Scientific) with PEI (Polyscience ...
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bioRxiv - Cell Biology 2023Quote: ... All the IHO1 truncations were expressed with the 3C HRV cleavable N-terminal MBP tag in suspension culture of High Five™ Cells (Invitrogen) in Sf-900™ III SFM medium ...
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bioRxiv - Plant Biology 2023Quote: ... OLV-FL or one of the truncated OLV versions were shuttled simultaneously with PYE into the destination vector pBiFC-2in1-NN (Grefen and Blatt, 2012) (N-terminal nYFP and cYFP fusions) via multisite LR reaction (Gateway, Thermo Fisher). Hereby pBiFC-2in1-NN:OLV-FL-PYE and pBiFC-2in1-NN:PYE-OLV-FL (additionally all truncated OLV versions were cloned into pBiFC-2in1-NN combined with PYE as described for OLV-FL ...
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bioRxiv - Plant Biology 2023Quote: ... For N-terminal fusions the pDONR entry clones with PYE and OLV sequences were used for Gateway LR reaction (Gateway, Thermofisher, Scientific) to generate the destination vectors based on pH7WGY2 (N-terminal YFP ...
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bioRxiv - Neuroscience 2023Quote: ... then reverse transcribed using random primers with a N-terminal amine group and Revert-aid H-minus reverse transcriptase (Thermo Fisher). On the second day ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Biophysics 2023Quote: Plasmids encoding and expressing M with an N-terminal 6xHis tag and with and without SUMO tag fusions were designed in VectorNTI software (ThermoFisher Scientific) and synthesized de novo by GENEWIZ/Azenta Gene Synthesis service ...
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bioRxiv - Immunology 2024Quote: Plasmodium falciparum 3D7 CSP was used as a reference to generate an N-terminal immunogen conjugated to KLH (Thermo Fisher Scientific). Murine studies were performed under approved protocol 22-0007 ...
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bioRxiv - Microbiology 2024Quote: ... the adaptor-complementary primer P1 (Supplementary Table S1) was used for reverse transcription of the terminal of each strand using SuperScript™ III Reverse Transcriptase (18080093, Invitrogen) at 50°C as described by the product instruction ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured as temperature increased 1°C/minute from 25°C to 95°C in a StepOne Plus qPCR instrument (Applied Biosystems). Protein from 2 independent purifications was used ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was run over either one (at 10°C) or two (at 10°C and 2°C) immobilized pepsin columns (Applied Biosystems; Poroszyme Immobilized Pepsin Cartridge ...
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bioRxiv - Microbiology 2021Quote: ... 96°C for 1min and 25 cycles (10sec at 96°C, 5sec at 50°C and 3min at 60°C) and a 2720 Thermal cycler (Applied Biosystems). For each RT-PCR product ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the samples were run on a thermocycler (60 min, 37°C; 5 min, 95°C; held, 4°C; Applied Biosystems); cDNA was stored at −20°C until use.
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bioRxiv - Cancer Biology 2021Quote: ... human melanocyte growth supplement (HMGS, Gibco), gentamicin (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... Pool A (human) (Thermo Fisher Scientific) using the TaqMan® microRNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... human skeletal myoblasts (Thermo Fisher Scientific), and mouse C3H muscle myoblasts (C2C12 cells ...
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bioRxiv - Cancer Biology 2019Quote: ... human keratinocyte growth supplement (ThermoFisher Scientific), and penicillin-streptomycin ...
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bioRxiv - Cell Biology 2019Quote: Episomal human iPSCs30,31 (Thermo Fisher Scientific) were maintained as a monolayer on Matrigel (Corning Life Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... with human melanocyte growth supplements (Gibco). Cell lines were incubated at 37 °C in 5% CO2.
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bioRxiv - Molecular Biology 2019Quote: ... Human colon total RNA (Thermofisher Scientific) was aliquoted at 13 µg/mL and stored at −20°C
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bioRxiv - Cancer Biology 2021Quote: Human melanocytes were trypsinized (Gibco, 25300062), quenched ...
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bioRxiv - Systems Biology 2021Quote: ... The human 62-plex (eBiosciences/Affymetrix) was utilized with the modifications described below ...
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bioRxiv - Bioengineering 2020Quote: ... PE-conjugated anti-human CXCR4 (Invitrogen), FITC-conjugated anti-human ACE2 (R&D Systems) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human TGF-β1 (Life Technologies) was prepared in sterile water with 0.1% BSA following the manufacturer’s instructions and diluted in 50:50 media.
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bioRxiv - Genomics 2021Quote: ... Human neural stem cells (NSCs) (Gibco, a kind gift from Raymond Poot ...
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bioRxiv - Cell Biology 2021Quote: ... and human ATF4 (Ambion, USA, 16708), and Scrambled (Negative control ...
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bioRxiv - Microbiology 2020Quote: Normal Human Dermal Fibroblasts (NHDF; Gibco) and IRF3 -/- cells were maintained in Dulbecco’s modified high glucose media (DMEM ...