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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from human hippocampal/frontal cortex samples and cultured cells was extracted with mirVana’s isolation kit (Ambion, TX, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Cell Biology 2024Quote: ... The process entailed cloning the various human ACTL7B gene variants into the pENTR TOPO vectors using the pENTR d-Topo cloning kit (Invitrogen), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... and incubated at 30 °C for 48 h in Bioscreen C (ThermoFisher), where the OD600 was measured at 1 h intervals ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-c-myc beads (Pierce Anti-c-Myc Agarose, Thermo Scientific), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... anti-c-myc beads (Pierce Anti-c-Myc magnetic beads, Thermo Scientific), and anti-HA beads (EZ view Red Anti-HA Affinity Gel ...
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bioRxiv - Plant Biology 2021Quote: ... CLSY3 and CLSY4 were amplified from wild-type genomic DNA (Table S15) and cloned into the pDONR-P4P1R vector using the Gateway BP Clonase II Enzyme kit (Invitrogen, Cat# 11789020). These donor plasmids were recombined with a pDONR221 vector containing the GUS gene ...
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bioRxiv - Developmental Biology 2021Quote: ... we generated a targeting vector by inserting deGFP in pCR®-Blunt II TOPO® (Zero Blunt TOPO PCR cloning kit, Invitrogen). Homology arms PCR-amplified from endogenous sequences upstream of the start codon and downstream of the stop codon from REX1 were cloned to flank pCR®-Blunt II TOPO®-deGFP ...
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bioRxiv - Molecular Biology 2021Quote: ... Northern blots were carried out using γ-32P 5’-end-labelled oligonucleotide probes or random primed probes prepared using α-ATP32 and the DECAprimeTM II labelling kit (ThermoFisher Scientific). Quantification of northern blots was performed using Storm 860 PhosphorImager (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes of agarose blots were hybridized with a probe against znuC1 generated by PCR using primers "probe znuC1 Hvo_2398 fw/rev" and genomic DNA as template and the product was labelled using [α-32P]-dCTP and the random primed DNA labelling kit DECAprime™II (Invitrogen). In addition ...
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bioRxiv - Neuroscience 2019Quote: ... PCR amplified DNA from T7E1 digest positive individuals of the G1 generation was cloned into Dual Promoter TA Cloning® Kit pCR®II Vector (Invitrogen; ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Taqman MicroRNA Reverse Transcription Kit and Taqman Universal Master Mix II with the TaqMan MicroRNA Assay (Applied Biosystems, Foster City, CA, USA) were to test miR-216a-5p expression ...
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bioRxiv - Bioengineering 2022Quote: ... two rounds cRNA synthesis starting with 5 ng of total RNA were performed using the MessageAmp II aRNA amplification kit (Life Technologies, AM1751) and MessageAmp II-biotin enhanced aRNA amplification kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... media was collected from each well at 24 h and virus RNA was isolated using MagMAX Virus/Pathogen II Nucleic Acid Isolation Kit (Thermo Fisher Scientific) and automated extraction was carried out using KingFisher™ Flex Purification System ...
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bioRxiv - Plant Biology 2020Quote: ... p35S:3xFLAG-GUS was prepared by LR-recombining pJ2B-3xFLAG 61 with pENTR GUS (from LR clonase II kit, 11791020, Thermo Fisher Scientific). To prepare the PAD4 expression construct for complementation ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into pCR-Blunt II-TOPO vector using the Zero blunt PCR cloning kit (Life Technologies, Invitrogen, catalog no. K2800-40). Plasmid DNA was harvested from bacterial colonies for Sanger-sequencing to determine the precise mutations ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 2 µg of total RNA using oligo(dT) primers and Superscript II First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Quantitative RT-PCR was performed with FastStart Universal SYBR Green Master Mix and a LightCycler 96 Real-Time PCR System (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... or 250 ng (Fig. 7) of RNA were used to synthesize cDNA with the SuperScript™ II Reverse Transcriptase kit (ThermoFisher Scientific). Real-time PCR was performed using 2µl of cDNA and QuantiTect SYBR green PCR master (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The purified fragments were cloned into the pCR® II TA vector using the TA cloning kit (Thermo Fisher Scientific, United States) and sequenced at the TsingKe Biotechnology (Nanjing ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Total RNA (2 μg) was reverse transcribed using a Superscript II® Reverse Transcriptase Kit and random primers (Invitrogen Canada, Burlington, ON). Reactions were incubated at 42°C for 50 min and at 90°C for 5 min ...
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bioRxiv - Genomics 2020Quote: Total RNA was reverse transcribed to synthesize the first-strand cDNA with random hexamers and Superscript II reverse transcriptase kit (Invitrogen, Carlsbad, USA). Sequencing was attempted on all samples regardless of Ct value including negative controls prepared from nuclease-free water and NA12878 human gDNA ...
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bioRxiv - Pathology 2022Quote: ... Sequencing of OXR1 cDNA from lymphocytes cDNA was prepared from fresh lymphocytes of patient II-4 and control using Maxima™ 1st strand cDNA synthesis kit (Thermo Scientific) and amplified by PCR using Phusion High-Fidelity PCR Master Mix (Thermo Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... subcloned it into pENTR1A at the EcoRI restriction sites using the In-Fusion HD Cloning Kit and then transferred it into the destination vector pMpGWB10465 using Gateway LR Clonase II Enzyme mix (Thermo Fisher Scientific). To construct proMpICE2:Citrine-GUS-NLS ...
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bioRxiv - Microbiology 2021Quote: ... The amplified DNA product was also cloned into pCR Blunt II TOPO vector using a Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific) and the spike NTD sequence of individual clones was determined by Sanger sequencing (Genewiz ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into pCR-Blunt II-TOPO vector using the Zero blunt PCR cloning kit (Life Technologies, Invitrogen, catalog no. K2800-40). Plasmid DNA was harvested from bacterial colonies for Sanger-sequencing to determine the precise mutations ...
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bioRxiv - Microbiology 2021Quote: ... We enriched the mRNA fraction and diluted inhibitory substances in the RNA extracts using the MessageAmp™ II-Bacteria RNA Amplification Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and Nr3c1 cDNAs were cloned into the lentiviral Gateway Vector using LR clonase II and a pENTR-D-TOPO cloning kit (Thermo Fisher Scientific). A mutant form of Nr2f6 (C112S ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... where the PSMB7 alleles are under the control of the constitutive GPD (TDH3) promoter (Gateway® LR Clonase® II enzyme mix kit from Invitrogen). The conditions for the error-prone PCR were selected to introduce 1-4 mutations per Kbp.
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... The verified hybrid human gene-yeast tail swap variants were cloned into the yeast expression vector pAG416GPD-ccdB (URA3; CEN6) by LR cloning (Gateway® LR Clonase® II enzyme mix kit from Invitrogen).
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bioRxiv - Plant Biology 2023Quote: ... the first-strand cDNA was synthesized using 2μg of DNase-treated total RNA as a template and superscript II from the reverse transcription kit (Invitrogen, Carlsbad CA, USA) follow to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The resulting 1291 bp product was cloned into PCR II TOPO vector using TOPO TA cloning kit (ThermoFisher Scientific, catalog number 461020) and sequenced with T7 and SP6 primers to confirm the sequence of FIN4 mRNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then counted and viability was checked using the Countess II automated cell counter and the readyprobes red/blue viability kit (Thermo Fisher Scientific). Libraries were prepared in accordance with the protocol for 10x Chromium Single Cell 3’ v2 (10x Genomics) ...
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bioRxiv - Microbiology 2023Quote: We adjusted RNA concentrations and performed Reverse-Transcription (RT) on extracted RNAs using a SuperScript II RT kit (Thermofisher, Waltham, Massachusetts, USA) and random primers (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: The RNA was measured with Qubit® RNA HS Assay Kits again and libraries were prepared with NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads (Invitrogen Life Technologies (Ambion Applied Biosystem) ...
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bioRxiv - Biochemistry 2023Quote: ... University of Freiburg and cloned into pCDNA5-FRT/To vector with a C-term 3xFLAG-HBH tag (kind gift from Ibrahim Avsar Ilik, Max Plank Institute, Freiburg) using Gateway LR clonase II enzyme kit (Life Technologies 11791020). pSupS1 and pJA2 M1 RNA were kindly provided by Prof ...
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bioRxiv - Microbiology 2023Quote: ... Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific, Waltham, MA, USA) and subsequently used for E ...
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bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... the PCR products from using the primer Cartpt-F and Cartpt-3’ junk-R 5’- GTAAGCTGAGGTGAAGCCAGACATgtcgac or Cartpt-5’ junk-F 5’- AGAGGGAATATGGGAACgaattcgc and Cartpt-R were subcloned into the pCR Blunt II TOPO vector (Zero Blunt TOPO PCR Cloning Kit, Thermo Fisher Scientific) and sequenced using M13-Foward and M13-Reverse primers.
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bioRxiv - Neuroscience 2024Quote: ... the PCR products from using the primer DBH-WT-F and DBH-3’junc-R 5’- TCTGAAGAATAGCTTCTCACAAgagctcag were subcloned into the pCR Blunt II TOPO vector (Zero Blunt TOPO PCR Cloning Kit, Thermo Fisher Scientific) and sequenced using M13-Foward and M13-Reverse primers.
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bioRxiv - Microbiology 2024Quote: ... and 1492R (5’-TACGGYTACCTTGTTACGACTT-3’) [29–31] with the Invitrogen Platinum II Taq Hot-Start DNA Polymerase kit (Cat. No. 14966001, Invitrogen, Waltham, MA). The 16S rRNA gene amplicons were sequenced by GENEWIZ (Azenta Life Sciences ...
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bioRxiv - Microbiology 2019Quote: ... Anti-c-Myc (Invitrogen), Anti-double stranded J2 antibodies (Scicons).
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bioRxiv - Microbiology 2021Quote: ... at 4°C (Nunc MaxiSorp flat bottom ...
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bioRxiv - Immunology 2021Quote: ... C media (ThermoFisher Scientific) and plated onto Luria-Bertani
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bioRxiv - Genetics 2022Quote: ... Gateway Cassette C (Invitrogen) was cloned into the p413 vector just downstream of (3’ to ...
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bioRxiv - Developmental Biology 2023Quote: ... 37°C (ThermoFisher, AM1350). The mRNA was purified (RNeasy Mini kit ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...