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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein was transferred (400 mAh, 60 min, 4 °C) in Towbin buffer onto PVDF membrane (Cat. No. 88518, Thermo Scientific). Total protein was quantified with Ponceau S stain ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C by adding 0.3 µg of Pierce trypsin protease (MS grade, Thermo Fisher Scientific), followed by a second incubation with 0.3 µg trypsin for three hours.
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bioRxiv - Immunology 2023Quote: ... added with 50 mM DTT for 10 min at 75 C and loaded onto precast NuPAGETM 4-12% bis-tris protein gels (Invitrogen), or NuPAGETM 12% bis-tris protein gels (these ones were used for LC3 immunoblotting ...
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bioRxiv - Cell Biology 2023Quote: ... Then the cell lysate was mixed with antibodies at 4 °C overnight followed by t he addition of protein G beads (10003D, Invitrogen). Otherwise ...
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bioRxiv - Molecular Biology 2023Quote: ... and supernatants were incubated (2 h, 4°C) with 5 µg antibodies conjugated to 25 µL protein G dynabeads (Invitrogen). Samples were immobilised ...
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bioRxiv - Developmental Biology 2022Quote: Protein lysates were denatured by heating at 70 °C for 30 min with NuPAGE LDS sample buffer (Invitrogen, cat. # NP0007) and NuPAGE reducing agent (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... All FCS measurements were carried out at ∼20 nM fluorescently labeled protein at 20°C in Nunc chambered coverslips (ThermoFisher). The chambers are pre-incubated with poly-lysine conjugated polyethylene glycol (PLL-PEG ...
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bioRxiv - Plant Biology 2023Quote: ... the denatured proteins were removed by centrifugation (4,122 g, 20 min, 4°C, Haraeus Multifuge X3R, Thermo Scientific Waltham, USA). To precipitate polysaccharides and AGPs ...
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bioRxiv - Plant Biology 2023Quote: ... the entry vector was subsequently recombined into the Gateway-compatible plant binary vectors pGWB505(48) for C-terminal fusion with EGFP (Enhanced Green Fluorescent Protein) through Gateway LR reaction (Invitrogen). The 1053 bp native promoter ...
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Identification of resistance mechanisms to small-molecule inhibition of TEAD-regulated transcriptionbioRxiv - Cancer Biology 2023Quote: ... at 4⁰C and centrifuged (18,000 x g and 4⁰C) for 10 minutes prior to protein quantification by BCA assay (ThermoFisher Scientific). Proteins in lysates (1µg/ml ...
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bioRxiv - Plant Biology 2023Quote: ... and RAF39 driven by the cauliflower mosaic virus (CaMV) 35S promoter and C-terminally tagged with green fluorescent protein (GFP) was achieved using Gateway LR Clonase II (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4°C with the corresponding antibody bound to protein A/G-magnetic beads (Dynabeads, Invitrogen). Immuno-complexes were washed 4 times with IP and twice time with kinase buffer (25 mM Hepes pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... The final SEC-buffer was either supplemented with 2 mM CaCl2 or concentrated proteins were dialysed overnight at 4 °C using 3.5K MWCO Slide-A-Lyzer MINI Dialysis Devices (Thermo Scientific) in 100 mL SEC buffer containing 2 mM CaCl2 ...
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bioRxiv - Genetics 2024Quote: ... pH 8.2 by beating with 0.5 mm glass beads at 4°C and protein concentration was determined by bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Protein was reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were centrifuged at 15,000xg for 15 min at 4°C and the supernatant was pre-cleared with protein G-magnetic Dynabeads (#10004D, Invitrogen) at 4°C for 2h ...
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bioRxiv - Cancer Biology 2024Quote: ... proteins eluted by heating at 95°C for 10 min with Tris Lysis Buffer and LDS Sample Buffer 4X (Invitrogen). For western blots ...
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bioRxiv - Immunology 2024Quote: ... The solid protein was denatured at 95°C for 5 min in NuPAGE LDS Sample Buffer (4X) (Thermo Scientific Inc) containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Molecular Biology 2024Quote: ... and 0.5% SDS) and incubated for 12-16 h at 4°C with 25 μL protein G Dynabeads (Invitrogen, 10004D) and 5 μg of antibody (Supplementary table 6) ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated for 16 hours at 4°C with HA Tag-precoated Dynabeads-Protein G (Invitrogen, Cat.#: 10007D). Then the Dynabeads were rinsed with a high salt buffer (50mM Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1200 µg of protein were incubated overnight rotating at 4°C with 200 µl of streptavidin beads (Thermo Scientific, 88816) which were acetylated with Sulfo-NHS-Acetate beforehand ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... T lymphocytes were purified using the Pan T cell isolation kit (Miltenyi Biotech) and activated with Dynabeads Human T-Activator CD3/CD28 (1:1 beads:cell) (ThermoFisher) in X-vivo 15 medium (Lonza ...
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bioRxiv - Immunology 2022Quote: Supernatants were collected 3 days post Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco) stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... slides were incubated for 1 hr with goat anti-human 555 (Thermo Fisher Scientific, A-21433; 1:1000 dilution) and goat anti-rabbit 488 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitation of endogenous PRRC2B was performed using 1 μg (dilution factor 1:125) rabbit anti-human PRRC2B antibody (Invitrogen) followed by Magnetic dynabead protein A (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The Macrophage panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:50, 48-3697-82, Invitrogen) and anti-mouse Ki67-PE/Cyanine7 (1:150 ...
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bioRxiv - Cancer Biology 2024Quote: ... while the MDSC panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:300, 48-3697-82, Invitrogen). The Macrophage panel’s intracellular isotype antibodies consisted of Rat IgG2a kappa ...
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bioRxiv - Immunology 2024Quote: ... primary human CD4 T cells were activated with anti-CD3+ anti-CD28 Dynabeads (1:1) (Thermofisher scientific, cat # 11161D) + IL-2 overnight and then 1 million aliquots of cells (triplicates ...
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bioRxiv - Cancer Biology 2021Quote: ... of Iowa) was prepared by diluting virus stocks to 400 p.f.u./µL into OptiMEM (Gibco) followed by addition of CaCl2 to a final concentration of 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... and transfected for virus reconstitution into BHK-21 cells with Lipofectamine 3000 (ThermoFisher #L3000-075).
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bioRxiv - Molecular Biology 2020Quote: ... a 20μL monoplex reaction contained 5μL of 4x TaqMan Fast Virus Master Mix (Thermo Fisher), 0.6µL of each primer (0.3μM final concentration each) ...
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bioRxiv - Immunology 2019Quote: ... two-fold virus stock dilutions were prepared in U-shaped 96-well plates (Thermo Scientific). The same volume of a 1% human O+ red blood cell suspension (Innovative Research ...
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bioRxiv - Microbiology 2019Quote: ... Other antibodies were obtained from commercial vendors as follows: Anti-coxsackie virus B3 antibodies (ThermoFisher), Anti-adenovirus A5 antibodies (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... RAW264.7 cells were then transduced with virus and selected using hygromycin at 10mg/ml (Invitrogen) to select for cells containing the shRNA plasmid.
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bioRxiv - Microbiology 2021Quote: ... and the number of virus particles was determined using a Nanodrop 2000 (Thermo Fisher Scientific). The adenovirus stocks were aliquoted and stored at -80°C.
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bioRxiv - Neuroscience 2021Quote: ... The final buffer for the virus was: dPBS + 5% Glycerol + 0.001 Pluronic F-68 (Invitrogen). The AAV titre (1.71 × 1013 GC/ml ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqManTM Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... The qPCR for Sendai virus detection was performed using TaqMan Gene Expression Assays (Life Technologies). Quantitative RT-PCR was performed using SYBR® Green PCR Master Mix (Applied Biosystems™ ...
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bioRxiv - Cell Biology 2022Quote: Cell lines were generated using Sendai virus CytoTune-iPS 2.0 Sendai reprogramming kit (Thermo Fisher) for transfection of Yamanaka’s factors ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated virus was run on a 4-20% Tris-Gly SDS-PAGE gel (Life Technologies), which was then analyzed using the Pierce Silver Stain Kit (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA was extracted from amplified virus stock using Trizol reagent (Invitrogen; Cat no. #15596018). Reverse transcription was carried out using in-house produced Moloney murine leukemia virus (MMLV ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV, Invitrogen). PCR was performed in an AriaMx thermal cycler (Agilent ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UKEi1-A was reprogrammed with a Sendai Virus strategy (CytoTune iPS Sendai Reprogramming Kit, ThermoFisher). These studies were approved by the Ethical Committee of the University Medical Center Hamburg-Eppendorf (Az ...
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bioRxiv - Immunology 2022Quote: ... RNA from virus-infected cells was isolated using PureLink RNA Mini Kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... genomic DNA was extracted from 1010 virus using the PureLinkTM Genomic DNA mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The virus was dialyzed with PBS supplemented with 0.05% of Pluronic F68 (Thermo Fisher Scientific) using 100-kDa filters (Millipore) ...