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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 13,000 rpm for 15 minutes at 40°C and then incubated with Protein G Dynabeads (Life Technologies) in the presence of an anti-Myc antibody (9E10 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were boiled at 95°C for 5 min before running on a NuPAGE 4-12% Bis-Tris protein gradient mini gel (Invitrogen) at 200 V for 5 min in MOPS running buffer (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: ... The samples were incubated at 98°C for 10 minutes and 10 μl of each mixture and a SeeBlue Plus2 pre-stained protein ladder (Thermofisher) were loaded onto a NuPAGE 4-12 % Bis-Tris polyacrylamide gel (ThermoFisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by a digestion with the Nuclease and Enhancer proteins at 42°C before being electrophoresed on a 10% TBE Gel (Thermofisher). The gel was then stained in 100 ml 1x TBE buffer with 2 μl of 10 mg/ml ethidium bromide for 5 min ...
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bioRxiv - Microbiology 2019Quote: ... Samples were heated in Laemmli buffer at 100°C for 5min before proteins were separated on gradient polyacrylamide gels (4-12%, NuPage, Invitrogen), which were run at 175V for 50min ...
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bioRxiv - Pathology 2020Quote: ... The samples were kept at -80°C until protein extraction using the PARIS™ kit (Thermo Fisher, Waltham, MA, USA). Protein concentration was quantified by BCA assay (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell debris was pelleted by centrifugation at 13,000g for 10 minutes at 4°C and the protein concentration measured by bicinchoninic acid (BCA) assay (Pierce, Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Protein lysates (10-15 mg, tissues) were then incubated overnight at 4 °C with antibody-coated dynabeads (Thermo Fisher Scientific). Bound immune complexes were washed twice with lysis buffer containing NP-40 ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein samples (7.5 μg) were incubated for 10 min at 70°C with Bolt LDS Sample Buffer (ThermoFisher Cat# B0007) and Bolt Reducing Agent (ThermoFisher Cat# B0004) ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 3 minutes at 95°C and loaded on NuPAGE 4 - 12 % Bis-Tris Mini Protein Gel (ThermoFisher Scientific). Proteins were separated by electrophoresis in NuPAGE MOPS SDS running buffer and transferred in Bjerrum Schafer-Nielsen transfer buffer (48 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Developmental Biology 2022Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were centrifuged at 14,000 rpm for 20 min at 4°C and cleared with protein A-Sepharose beads (Thermo Scientific). Pre-cleared lysates were then incubated with anti-GluN2A-pS1459 coupled to protein A-Sepharose overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted cell lysates were incubated for 16 h at 4°C with Dynabeads Protein G (100-O3D, Thermo Fisher Scientific) precoated with 5 μL of anti-MYC antibody (ab32 ...
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bioRxiv - Biochemistry 2021Quote: ... Solubilised protein was cleared by centrifugation (10 min, 125000xg, 4°C) and incubated with antibodies cross-linked to Dynabeads (Invitrogen) by overhead rotation for 2 h on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mg of pre-cleared HeLa nuclear extracts were incubated overnight at 4°C with either 40 μg ATRX antibody or rabbit IgG cross-linked to Dynabeads Protein G (Invitrogen). Beads were washed three times with 5 ml wash buffer (20 mM HEPES ...
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bioRxiv - Genetics 2022Quote: ... Lysates were centrifuged at 15,000g for 15 min at 4 °C and quantified by the Bradford Protein Assay (Thermo Scientific). SDS-PAGE gel was used for protein electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were clarified by centrifugation at 20,800 g at 4°C for 10 min and supernatant protein concentrations quantified using the Bradford assay kit (ThermoFisher, #23236)
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bioRxiv - Cell Biology 2019Quote: ... Infected Sf9 cells were grown in spinner culture for 48–96 h at 27°C and His-tagged protein-purified using Ni2+-NTA agarose (Invitrogen) according to standard procedures ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysed cells were then centrifuged at 14,000 g for 15 min at 4°C and the supernatant was added to pre-washed and antibody-coupled Protein G Dynabeads (Invitrogen). For each RIP ...
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bioRxiv - Cell Biology 2020Quote: ... were denatured at 95°C for 5min and run on NuPAGE(tm) 4-12% Bis-Tris Midi Protein Gels (Invitrogen) at 200V for 1h ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear lysates were incubated overnight at 4°C with/without the indicated antibody then immune complexes were captured by addition of protein A/G magnetic beads (ThermoFisher) for 2h at RT ...
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bioRxiv - Genetics 2019Quote: ... 550 µl of cell lysates were pre-incubated with the following antibodies for hours at 4°C prior to overnight incubation under rotation with magnetic Dynabeads-protein-G (Invitrogen): for ORC ChIP ...
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bioRxiv - Molecular Biology 2020Quote: ... Six hundred μg of the supernatant were incubated for 4 hours under rotation at 4°C with 20 μl of protein A Dynabeads (Invitrogen) containing anti-UNR antibody or rabbit IgG as control in a final volume of 600 μl ...
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bioRxiv - Systems Biology 2019Quote: ... The protein pellet was resuspended and incubated at 65°C in 20 μl 1x NuPage loading buffer (Thermo Scientific, NP0007), resolved on 4–12% NuPAGE gels (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then incubated at 37°C for 30 minutes and loaded directly into a NuPAGE 4-12% Bis-Tris protein gel (Thermofisher) or stored at −20° C.
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bioRxiv - Microbiology 2020Quote: ... Lysate was centrifuged for 10 min at 10,000 × g at 4°C and protein concentration in the supernatant measured by BCA assay (Thermo scientific). Lysate equivalent to 5 mg of total protein was incubated with 30 μl anti-myc magnetic beads (Thermo scientific ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were incubated overnight on wheel at 4°C with immunoprecipitation buffer containing magnetic Protein A DynaBeads (Invitrogen, ThermoFisher Scientific) conjugated with human PKR antibody (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were incubated overnight on wheel at 4°C with immunoprecipitation buffer containing magnetic Protein A DynaBeads (Invitrogen, ThermoFisher Scientific) conjugated with human PKR antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-OC43-S or HCoV-HKU1-S C-terminally fused to the green fluorescence protein (GFP) using Lipofectamine 2000 (Invitrogen). Two days post transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were subjected to centrifugation at 14,000 × g and the supernatant was stored at −80 °C or collected for protein quantification using a BCA assay kit according to the manufacturer’s instructions (ThermoFisher 23227). Equal volumes of whole-cell protein extract were subjected to electrophoresis through denaturing 10% bis-Tris polyacrylamide gels and transferred to PVDF membrane ...
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... truncation and a TEV protease cleavage site followed by a double maltose-binding protein-(MBP) tag at its C terminus was cloned into the pFastBac vector (Invitrogen). To obtain a SV1–Gs complex with good homogeneity and stability ...
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bioRxiv - Immunology 2020Quote: ... then incubated another 5 hr at 37 C with additional Protein Transport Inhibitor Cocktail (eBioscience 00-4980-03, Thermo Fisher). Cells were then fixed and permeabilized with BD Cytofix/Cytoperm Kit (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed in 1x Laemmli Buffer pre-warmed to 95°C and the total protein concentration was measure using Nanodrop (Thermofisher). Proteins (15μg/sample ...
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bioRxiv - Molecular Biology 2022Quote: ... PABP-GFP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-GFP (clones 19F7 and 19C8 ...
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bioRxiv - Biochemistry 2022Quote: ... 1% (v/v) Triton X-100 and proteins eluted by resuspension for 10 min at 37 °C in LDS sample buffer (Invitrogen) diluted four-fold in 2.5% (v/v ...
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bioRxiv - Evolutionary Biology 2022Quote: We synthesized the Bowhead whale CDKN2CRTG protein with mouse codon usage (GeneScript) and directly cloned the synthesized gene into the pcDNA3.1-C-eGFP vector (Life Technologies), which added a C-terminal eGFP tag ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were centrifuged (13,000 × g for 5 min at 4°C) and supernatants are precleared with 10 μl protein G beads (Invitrogen; 1004D) at 4°C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 16000g for 20 min at 4°C and Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) was used to determine cleared lysate concentration ...
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bioRxiv - Plant Biology 2022Quote: ... and immunocomplexes were next incubated for 1h at 4°C with 40 μL of Dynabeads Protein A (Thermo Fisher Scientific). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... endogenous PABP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-PABP (clone 10E10 ...
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bioRxiv - Developmental Biology 2022Quote: ... 4°C and supernatant was collected and the protein content was quantified with a BCA kit (Thermo Fisher Scientific, 23225). Equal amount of protein was denatured by mixing with 4x Laemmli buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: The transfection of a mixture of specific siRNA against c-Myc protein (exon 2 and exon 3, IDs s9129 and s9130, respectively; Ambion) and DOT1L protein (IDs s39011 ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were centrifuged for 25 mins at 35,000 x g and the supernatant was adjusted to approximately 3 mg/ml protein (Nanodrop 2000 C, Thermofisher Scientific). To capture the SVs for content detection ...
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bioRxiv - Plant Biology 2024Quote: ... Protein-DNA complexes were immunoprecipitated by incubating the diluted chromatin overnight at 4°C with 15 μl of Dynabeads Protein-G (Invitrogen) equilibrated with 2 μg of anti-GFP antibody (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein was transferred (400 mAh, 60 min, 4 °C) in Towbin buffer onto PVDF membrane (Cat. No. 88518, Thermo Scientific). Total protein was quantified with Ponceau S stain ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C by adding 0.3 µg of Pierce trypsin protease (MS grade, Thermo Fisher Scientific), followed by a second incubation with 0.3 µg trypsin for three hours.