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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Lysates were then incubated overnight at 4 °C with 5 μg of prebound anti-SP100 or rabbit IgG isotype control protein A dynabeads (Thermo Fisher). The next morning ...
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cell extracts and purified protein samples were boiled at 95°C for 5 minutes followed by fractionation on NuPAGE Bis-Tris polyacrylamide gels (Thermo Scientific). Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of total protein were mixed with 4X NuPAGE LDS sample buffer and 10X NuPAGE reducing agent and subsequently denatured for ten minutes at 95°C with a thermo mixer and proteins were separated by size using 4-12% Bis-Tris Plus Gels (Life Technologies) in a SDS Polyacrylamide Gel Electrophoresis System (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... at 4 °C and the soluble protein in the supernatant was separated from the debris by centrifugation (13000 rpm, 20 minutes, 4 °C in Thermo Scientific™ Fiberlite™ F12-6 × 500 Fixed-Angle Rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4-20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... elution of proteins was carried out by incubating the beads at 80 °C with 2.5X NuPAGE LDS sample buffer (NP0007, Thermo Fisher Scientific). Samples were loaded on 4-12% NuPAGE Bis-Tris polyacrylamide gel (NP0322 ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µg of the proteins was incubated with equivalent amount of GST-α synuclein fusion protein at 4°C overnight in the present of Ni-NTA beads (Invitrogen). The pull-downs were washed 4 times with lysis buffer and boiled in 2X Laemmli sample buffer for five minutes.
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bioRxiv - Genetics 2023Quote: ... Extracts were incubated with rotation with rabbit polyclonal anti-Piwi antibodies (4 µg per sample) at 4°C for 4h followed by overnight incubation with DynabeadsTM Protein A (50 µl, Invitrogen, 10002D) at 4°C with rotation ...
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bioRxiv - Biophysics 2023Quote: ... heated to 95 °C for 5 min and loaded onto the gel with PageRuler™ Prestained Protein Ladder (Thermo Fisher Scientific) as a reference marker ...
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bioRxiv - Immunology 2023Quote: ... then S-glutathionylated proteins were released and prepared for SDS polyacrylamide gel electrophoresis (SDS-PAGE) with reducing sample buffer at 100°C (ThermoFisher #39000); immunoblotting was performed with anti-PDCE2 antibodies (GeneTex #GTX109766) ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were performed overnight at 4°C on 40μg of chromatin with antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific). The antibodies used are listed in Table S10 ...
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4 °C with mouse anti-ee antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific( ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were washed twice with PNK buffer and protein-RNA complexes were eluted for 10 minutes at 70°C in 1x LDS buffer (Invitrogen, NP0007) containing 25mM DTT ...
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Comparative Proteomic Study of Retinal Ganglion Cells Undergoing Various Types of Cellular StressorsbioRxiv - Molecular Biology 2023Quote: ... and reduced with DTT then denatured at 70°C for 10min prior to loading 20μg onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, Cat.# NP0315BOX) and separated appropriately (@ 200 constant V) ...
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bioRxiv - Immunology 2023Quote: ... and urine samples were stored at -80°C and analyzed to determine urinary protein concentration by a Bradford assay using the Pierce detergent compatible kit (#23246, ThermoFisher Scientific). The 24-hr urine protein amount was calculated based on 24-hr urine volume and total urinary protein concentration.
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bioRxiv - Molecular Biology 2023Quote: ... 1 mg of cell lysate was immunoprecipitated overnight at 4° C under rotation with Dynabeads Protein A (1.5 mg) (Thermo Fisher Scientific) and 10 µg anti-ZZZ3 antibody (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... Lysates from the secretomes and intracellular fractions were centrifuged (10 min, 14,000 g, 4 °C) and soluble protein concentrations were determined (Bradford assay, Thermo Fisher Scientific). For digestion ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged at 300g for 5 minutes at 4°C and homogenized in cold N-PER Neuronal Protein Extraction Reagent (Thermo Fisher) or cold RIPA lysis buffer (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... and then incubated for another 5 hours at 37 °C with additional Protein Transport Inhibitor Cocktail containing Brefeldin A and Monensin (eBioscience 00-4980-03, Thermo Fisher). Cells were then fixed and permeabilized with BD Cytofix/Cytoperm Kit (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Lysates were centrifuged for at 13,000 RPM 15 minutes at 4°C to recover supernatant and quantified for protein concentration by BCA assay (Thermo Scientific, 23225). 70µg of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... snap-frozen lungs were homogenized at 4°C at 20% w/v in protein extraction reagent (T-PER, Thermo Fisher Scientific) containing Halt™ Protease Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 100 ng of protein per well was coated overnight at 4°C onto 96-well NUNC MaxiSorp plates (Thermo Fisher Scientific). Coated plates were washed 3x using PBS and subsequently blocked using PBS supplemented with 2% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was precleared for on a rotating wheel at 16 rpm 30 min at 4°C with 10 µl of Protein G dynabeads (Thermo Fisher) that were previously washed with 100 µl of RIPA buffer (10□mM Tris–HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody or IgG (as control) was added and incubated for 4 hrs at 4°C with Protein G Dynabeads (Life Technologies) for antigen coupling ...
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bioRxiv - Molecular Biology 2024Quote: ... and denatured at 70°C for 10 min prior to loading onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, NP0315BOX) and separated half way (15min at 200 constant voltage) ...
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bioRxiv - Genomics 2024Quote: ... Proteins were then digested by 1h incubation at 55°C with 4 μl of 20 mg/ml Proteinase K (Ambion, AM2546). DNA was then recovered by phenol/chloroform extraction and ethanol precipitation ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... cells were isolated from human foreskin tissue and cultivated at 37 °C and 5% CO2 in Eagle’s minimal essential medium (Gibco, Thermo Fisher Scientific Inc., Waltham, MA, USA) containing 7 % fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from purified virus using the GeneJET genomic DNA purification kit (Thermo Fisher), and the fidelity of the changed regions was verified by Sanger sequencing ...
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bioRxiv - Genetics 2021Quote: RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus (M-MLV; Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... wt Flock House virus (FHV) was generated by transfecting S2 cells with pMT plasmid vectors (Invitrogen) containing respective genomes (NC_004146 for RNA 1 ...
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bioRxiv - Biochemistry 2019Quote: ... 293FT producer cells with ViraPower packaging mix (pLP1, pLP2, and pLP/vesicular stomatitis virus; ViraPower; Invitrogen) to generate the lentivirus according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... cells were washed 3 times and supplemented with virus production serum-free media (VP-SFM, Gibco) containing 0.6 µg/ml trypsin ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... Clarified virus working stock supernatant was overlaid onto a 25% sucrose-NTE (100nM NaCl (ThermoFisher Scientific), 10mM Tris (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... Reprogramming to pluripotency was carried out using the CytoTune 2.0 sendai virus kit (Thermo-Fisher Scientific) to overexpress four transcription factors (OCT4 ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated virus stocks in reduced-serum DMEM (2% FBS) supplemented with 50mM HEPES buffer (Gibco #15630080) were stored at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... unbound virus was removed by washing three times with PBS (Life Technologies, Carlsbad, California, United States). Next ...
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bioRxiv - Microbiology 2020Quote: ... The virus in the growth medium was recovered by Polyethylene Glycol 8000 (PEG-8000, Fisher Scientific) precipitation ...
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bioRxiv - Biophysics 2020Quote: Virus was attached to a gold-coated probe using dithiobis succinimidyl propionate (DSP) linkers (Thermo Scientific). Dithiobis succinimidyl propionate (DSP ...
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bioRxiv - Cell Biology 2020Quote: ... and sendai-virus iPSCs were cultured on matrigel-coated plates (Geltrex™ LDEV-Free, ThermoFisher Scientific) in Essential 8™ medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from virus-containing medium or cell lysates using TRIzol LS (ThermoFisher Scientific, #10296010) according to the manufacturer’s instructions and stored at −80°C for RT-qPCR ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was reverse transcribed using Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific), in the presence of oligo (dT)15 primers ...
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bioRxiv - Genomics 2021Quote: ... we reprogrammed fibroblast samples from 139 iPSCORE individuals using non-integrative Cytotune Sendai virus (Life Technologies) and the 149 iPSCs (7 subjects had 2 or more clones each ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from 100 µl of virus stock using a KingFisher Flex (Thermo Fisher Scientific) in combination with the MagMax Viral Pathogen kit II (Thermo Fisher Scientific) ...