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Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to a heating ramp from 25°C to 95°C at a rate of 1°C/s using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, USA). Data was analyzed by Protein Thermal Shift Software v1.4 to obtain melting temperature (Tm).
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bioRxiv - Cell Biology 2023Quote: ... The lysates were digested overnight at 37°C with 1-2 µL of the Lys-C (125-05061, Wako) and trypsin mixtures (Pierce, Thermo Fisher Scientific) (10 ng/μL each) ...
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bioRxiv - Immunology 2023Quote: ... followed by 40 cycles at 95°C for 15 sec then 60°C for 1 min using a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Extracted MAC DNA was used as the quantification standard.
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bioRxiv - Cancer Biology 2022Quote: ... The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938). The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cas9 over expressing cells were transduced with lentiviruses expressing sgRNA targeting human hypoxanthine phosphoribosyltransferase 1 (HPRT1) (LentiArray™ CRISPR Positive Control Lentivirus, human HPRT, Thermo Fisher Scientific Cat # A32829). HPRT1 knockout cells shows resistance to 6-thioguanine (6TG ...
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bioRxiv - Plant Biology 2023Quote: ... Tween20) for 1 h at 25 °C or overnight at 4 °C before labelling with the primary antibody (α-GSH, ThermoFisher, MA1-7620, 1:1000 in 2.5% (w/v) milk powder dissolved in TBS-T) ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
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bioRxiv - Genetics 2021Quote: ... donkey-anti-rabbit and mouse-anti-human IgG secondary antibodies (Molecular Probes, 1:1000), or NbALFA-Atto647 (NanoTag Biotechnologies ...
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bioRxiv - Developmental Biology 2021Quote: ... or of the rabbit anti-Human Rac1 polyclonal antibodies (1:40, Thermofisher PA1-091) were added in 1 % BSA-PBS and incubated O/N at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Systems Biology 2021Quote: ... followed by the secondary goat anti-human HRP-conjugated antibody (1:4000, A18805, Invitrogen). Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... anti-CD9 (1:500, mouse monoclonal anti-human CD9, clone MM2/57, Invitrogen, AHS0902). The membranes were then washed three times with 0.1% Tween 20 in PBS for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: Human primary fibroblasts FIB-1 and FIB-2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS and Pen-Strep antibiotics ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 633-conjugated goat anti-human antibody (1:500; A21091 from Life Technologies); Alexa Fluor 555-conjugated goat anti-rabbit (1:500 ...
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bioRxiv - Bioengineering 2020Quote: ... Viable human tumor tissue pieces of ∼1-2mm3 were thawed in prewarmed DMEM (Gibco) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2019Quote: The human colorectal cancer cell line DLD-1 was cultured in DMEM (Life Technologies) supplemented with heat-inactivated 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Systems Biology 2020Quote: ... The human monocytic cell line THP-1 was maintained in RPMI 1640 medium (Gibco). All media were supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Human IgG Fc HRP Secondary Antibody (ThermoFisher Scientific Catalog #31423, 1:10,000), and Rabbit anti-Human IgG F(ab’)2 HRP Secondary Antibody (ThermoFisher Scientific Catalog #31482 ...
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bioRxiv - Biochemistry 2021Quote: ... and secondary anti-human IgG antibodies (1:2000) conjugated to Alexa fluor 488 (Invitrogen). Cells staining positive for Spike were measured by flow cytometry on a Becton Dickinson BD LSR II Special Order System Flow Cytometer With HTS Sampler ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 647–conjugated goat anti-human (1:1000; A-21445; Thermo Fisher Scientific). Secondary antibodies were dilute in PBST and incubated for 30 minutes at RT ...
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bioRxiv - Microbiology 2022Quote: ... stained using FITC-conjugated F(ab’)2-Goat anti-human IgA (Invitrogen; 1/1000) in PBS + 0.1% BSA for 20 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa fluor 488 Goat anti-Human IgG Secondary (#A-11013, dilution 1:100, Invitrogen); APC anti-human CD4 Antibody (#357408 ...
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bioRxiv - Immunology 2024Quote: ... and anti-human IgG Alexa Fluor™ 647 (ThermoFisher, Cat. #A-21445, 1:3000) for 10 min at room temperature in dark ...
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bioRxiv - Microbiology 2024Quote: ... Human THP-1 monocytes obtained from ATCC were grown in RPMI 1640 medium (Gibco) containing 2 mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: ... incubated with a 1:10,000 dilution goat anti-human IgG (H+L)-HRP (Invitrogen), and washed twice more before detection with ECL (ECL Plus Western Blotting Substrate ...
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bioRxiv - Microbiology 2023Quote: ... AlexaFluor 647-conjugated anti-human and AlexaFluor 594-conjugated anti-mouse (Invitrogen, 1:1,000) antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... and an Alexa Fluor 488- conjugated goat anti-human secondary antibody (1:200, Invitrogen) were then used to reveal the immunopositive structure ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Biophysics 2023Quote: The gene encoding human A2AAR (1-316) was cloned into a pPIC9K vector (Invitrogen) at the BamHI and NotI restriction sites ...
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bioRxiv - Genomics 2023Quote: A serial dilution from 1 ng to 1,000 ng Human Liver Total RNA (ThermoFisher) was prepared to generate the various input samples ...
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bioRxiv - Physiology 2024Quote: ... and C3 (rabbit anti human, 1:100, cat. no.: PA5-21349, Thermo Fisher Scientific). Thereafter ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant human PDZD8 (1-28) - FLAG was expressed in Expi293 Cells (Thermo Fisher Scientific) using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Human foreskin fibroblast (HFF-1) cells (ATCC SCRC-1041) were maintained in DMEM (Gibco) with 20% FBS ...
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bioRxiv - Physiology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco, cat no. 11875093) supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2021Quote: ... Human recombinant fibroblast growth factor (human FGF2) (10 ng/ml, Invitrogen) was added to Dulbecco’s Modified Eagle’s Medium (DMEM)/F-12 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used for human samples were anti-human CD31 (#17031942, Invitrogen), anti-human CD45 (#17945942 ...
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bioRxiv - Immunology 2020Quote: ... Alexa-Fluor 555 conjugate secondary antibodies (Thermo Fisher Scientific, USA, 1:400, 1 h at 37°C) were used for labelling Spike ...
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bioRxiv - Molecular Biology 2023Quote: ... and BE(2)-C were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Bioengineering 2023Quote: Hydrogel-embedded PCLS punches were maintained at 37°C with 5.0% CO2 in DMEM:F12 (1:1; Gibco) media with 0.1% fetal bovine serum (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... A preheated (90°C, 1 min) number 1 German borosilicate sterile 2 well chamber coverglass slide (ThermoFisher) was loaded with 750 μL of melted 0.7% Omnipur agarose (EMD Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Plant Biology 2019Quote: Trypsin or Lys-C digested protein samples were analyzed by a Q Exactive mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) connected to Easy NanoLC 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 was isolated from 0.35 mg of lysate by 90 min incubation at 4°C with 20 µl Protein-G Dynabeads (ThermoFisher, 10004D) and 2 µg of each of three anti-CDC20 mAbs ...
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bioRxiv - Immunology 2021Quote: Splenocytes were processed as described in the previous section and stimulated with RBD peptides for 5 hours at 37°C with protein transport inhibitor (Invitrogen) and anti-mouse CD107a-FITC antibody (BioLegend) ...
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bioRxiv - Biochemistry 2020Quote: Protein samples were heated at 95°C for 5 minutes before loading and separated onto SDS-PAGE cassette (Thermo Fisher Scientific Bolt™ 4-12% Bis-Tris Plus Gels ...
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bioRxiv - Developmental Biology 2021Quote: ... at 4°C for 2 hours with gentle agitation and subsequently with 45 μL Dynabeads Protein G (Thermo Fisher Scientific) at 4°C overnight with gentle agitation ...