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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Anti-Human-HRP (Invitrogen) was diluted 1:5000 in 1% BSA/PBS ...
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bioRxiv - Cell Biology 2024Quote: ... human recombinant insulin (ThermoFisher) was added at 100 nM for 30 minutes and Smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2024Quote: ... human CD3-PE (Invitrogen), and mouse CD45-erpCP Cy5.5 (BD Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... Precleared nuclear protein lysates (500 μg) were incubated with 20 μl of anti-c-MYC tag antibody coupled to magnetic beads (Thermo Scientific). After two hours ...
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bioRxiv - Cell Biology 2019Quote: ... the cell lysates were centrifuged at 12,000 g for 15 min at 4 °C and the supernatant was precleared with Pierce Protein A/G Magnetic Beads (88802, Thermo scientific) for 15 min on ice and then incubated with 2 μg anti-CRIPT antibody at 4 °C for 2 hours and with Pierce Protein A/G Magnetic Beads for another 2 hours ...
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bioRxiv - Immunology 2021Quote: ... 1 × 106 isolated T cells were then stimulated at 37°C with eBioscience™ Cell Stimulation Cocktail plus protein transport inhibitors (Invitrogen) for 6 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and bound proteins eluted by heating at 70200°C for 10 minutes in 20 μl 4x Nupage LDS loading buffer (Invitrogen, NP0007) containing 5% β-mercaptoethanol ...
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bioRxiv - Biophysics 2022Quote: Samples were heated at 95°C for 5 minutes before loading the onto the PAA-gel (marker: PageRuler Plus Prestained protein ladder (Thermo Scientific)) ...
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bioRxiv - Cancer Biology 2019Quote: ... Total cell extracts were then centrifuged at 10000g at 4°C and supernatants were assessed for protein concentration using the BCA assay kit (Pierce, Thermo Scientific) according to the manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... Gastric homogenates were centrifuged at 13,000 × g at 4°C and the supernatants collected for analysis by the Qubit™ protein assay (ThermoFisher Scientific) and ELISA.
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bioRxiv - Developmental Biology 2019Quote: ... The lysate was centrifuged at 16,000 X g for 15 min at 4°C to remove cell debris and proteins were quantified using a BCA assay kit (Pierce/Thermo Scientific). Thirty or 40 µg of protein was resolved on Mini-PROTEAN TGX precast 12% SDS-PAGE gels (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... was incubated with 20 μg of NKp46-IgG1Fc or NKG2D-IgG1Fc fusion protein at 4° C with rotary agitation for 16 h and then incubated with 2 mM of DTSSP crosslinker (ThermoFisher Scientific) for 2 h on ice ...
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bioRxiv - Synthetic Biology 2021Quote: ... Immunoprecipitation was performed for 16h at 4°C on a rotating platform by incubating with Dynabeads protein-G (Thermo Fisher Scientific) conjugated with antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... These proteins were then reduced at 90°C for 10 min and loaded into a 4-12% bis-tris gel (Life Technologies). This gel was run for 5 min at 150 V in 1x MOPS buffer and stained using Coomassie blue (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunoprecipitation was performed for 16h at 4°C on a rotating platform by incubating with Dynabeads protein-G (Thermo Fisher Scientific) conjugated with 5 μg of one of the following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... and 30 μg (iPSCs-MNs) protein of each sample was denatured for 10 min at 70 °C in LDS sample buffer (Invitrogen NP0008) containing 2.5% 2- mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were then boiled at 100 °C for 10 min and 5 μl of each sample was used for protein quantification using the MicroBCA assay (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Biotinylated proteins were isolated by incubating the samples with equal total protein amounts overnight at 4°C and gentle agitation with 30 μl of Neutravidin-magnetic beads (Fisher Scientific, Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Thawed cell pellets were heated 5 min at 95°C before measuring protein contend by using a micro-BCA assay (Pierce, ThermoFisher Scientific) and a NanoDrop One spectrophometer (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... The supernatants of lysates were mixed with anti-c-Myc antibody (Nacalai tesque) attached to Protein G DynabeadsTM (Thermo Fisher, 10004D). After incubation for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were centrifuged (14000 g, 30 min, 4 °C) and protein concentration in the supernatant was determined with the BCA method (ThermoFisher, Germany). After boiling with 4x Laemmli buffer (1 M Tris pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2022Quote: ... then the supernatant was diluted 10x and pre-cleared for 30 min at 4 °C with rotation using 5 µl protein A- and G-coupled dynabeads (ThermoFisher Scientific). An input sample was taken ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hr at 4°C, then proceeded for immunoprecipitation with specific antibodies (Rab5A, Rab 11 and Rab7) and Protein A/G bead (Thermo Fisher) for overnight at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Microbiology 2019Quote: ... and denatured (10 min, 95 °C) Approximately 7.5 µg of total protein (as determined by A280 on a Nanodrop; Thermo Fisher Scientific) was loaded onto 8-16% TGX gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were centrifuged (14.000x g, 10 min at 4°C) and soluble protein concentrations were determined by a Bradford assay (Thermo Fisher Scientific). For digestion ...
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bioRxiv - Microbiology 2020Quote: ... Two and a half micro liter of pBAD-C+SPA+gst sample as well as 5 μL of Spectra Multicolor Low Range Protein Ladder (Thermo Scientific) and 10 μl of samples or controls were loaded on tricine-SDS-gels (Schägger ...
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C on rotation and for one additional hour with mixed Dynabead protein G and A (20:10 µl, Life Technologies). Beads were washed with TSE- 150 (0.1% SDS,1% Triton ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The lysate was clarified via centrifugation at 16,100 × g for 10 min at 4°C then total protein concentration was measured using a detergent-compatible Bradford assay (ThermoFisher 23246). Lysate was transferred to a 384-well plate (30 μL × 3 TR-FRET replicates ...
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bioRxiv - Microbiology 2020Quote: ... and pcDNA3.1 SARS-CoV-2 spikeΔ19 plasmid (encoding SARS-CoV-2 spike protein, with 19 AA truncated in C terminal) using Lipofectamine 3000 (Invitrogen, L3000-015) following the manufacturer’s instruction ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... Elution fractions were tested on 12% SDS-PAGE gel and fractions containing protein were pooled and dialyzed overnight at 4 °C using Snakeskin dialysis tubing 10K MWCO (Thermo Scientific) in kinase storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Bacterial lysate was clarified by centrifugation at 40,000 xg for 45 min at 4°C and the clarified protein bound to glutathione agarose beads (Thermo Fisher Scientific) at 4 °C for 4 hours with rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Soluble proteins were retrieved after centrifugation at 15000 g at 4°C for 10 minutes and quantified using the Coomassie protein assay (Thermo Scientific). 25 µg of proteins were separated on a NuPAGE 4% -12% gel (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Biochemistry 2022Quote: MT-4 cells with and without lentiviral plasmids were collected by centrifugation (225× g, 5 min, at 20 °C) and proteins were extracted using lysis buffer (RIPA Thermo Scientific, cOmplete™ ...
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bioRxiv - Cancer Biology 2022Quote: ... sonicated chromatin was incubated overnight at 4°C with antibodies that had first been coupled to Protein G beads (Thermo Fisher). After incubation ...
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bioRxiv - Immunology 2022Quote: ... 700 μg of protein were mixed and rotated with 0.5 μg of STING antibody for 3 h at 4 °C after which 25 μL of Dynabeads Protein G (Invitrogen, 10004D) were added to each sample and rotated for an additional hour ...
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bioRxiv - Microbiology 2022Quote: ... Filtered supernatant was then concentrated by spinning at 4°C 3000 rcf for 30 min using Pierce Protein Concentrator (ThermoFisher, 88537). Virus aliquots were stored long-term at −80°C ...
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bioRxiv - Microbiology 2022Quote: ... and heated at 70°C for 10 min before being loaded onto NuPAGE 4 to 12% bis-Tris protein gels (Life Technologies). The proteins were transferred using iBlot™ 2 Transfer Stacks (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Invitrogen, 10001D). The following antibodies was used ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were cleared by centrifugation at 21,000 x g for 10 minutes at 4°C and protein concentration was determined by Bicinchoninic acid assay (Pierce, ThermoFisher Scientific). Proteins were reduced with 5 mM dithiothreitol for 30 minutes at 55°C ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Neuroscience 2022Quote: ... followed by overnight incubation at 4°C with either 10 μl of pre-equilibrated protein A or G Ultralink beads (Thermo Scientific). The resin was washed several times with immunoprecipitation buffer spinning at 700 g for 30 sec ...
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bioRxiv - Neuroscience 2022Quote: ... RIPA-soluble extracts were clarified by centrifugation at 100,000 g for 30 min at 4°C in a Beckman Ultracentrifuge and protein content was measured using a detergent-compatible Bradford assay (Thermo Fisher).
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Molecular Biology 2024Quote: ... cell extracts and purified protein samples were boiled at 95°C for 5 minutes followed by fractionation on NuPAGE Bis-Tris polyacrylamide gels (Thermo Scientific). Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein lysates were heat-denatured for 10 min at 95°C with the addition of Bolt LDS buffer (Thermo Fisher Scientific) and Bolt Reducing agent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 5-10 ug of protein lysates of each sample was denatured for 10 min at 70 °C in LDS sample buffer (Invitrogen NP0008) containing 2.5% 2-mercaptoethanol (Sigma-Aldrich) ...