Labshake search
Citations for Thermo Fisher :
1351 - 1400 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Following preset incubation times (0, 0.5, 1, 3, or 5 h) cells were harvested by trypsinization (500 μl TrypLE, Gibco) for 5 min at 37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... approximately 1 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, Thermo Fisher Scientific) and separated over a C18 column (50 cm × 75 μm ...
-
bioRxiv - Cell Biology 2023Quote: ... the samples were incubated with DAPI (1/25,000 in PBS) for 5 min and mounted with Prolong Diamond antifade mounting medium (Invitrogen). The samples were imaged and analyzed with C-Plan Apochromat 63x/1.4 Oil DIC M27 objective under confocal microscope (LSM780NLO ...
-
Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... sections were washed three times for 5 min in PBS (0.1 M, pH 7.4) and incubated with rabbit anti-GFP (1:500; Invitrogen, A11122) and chicken anti-GFAP (1:500 ...
-
bioRxiv - Neuroscience 2023Quote: ... Neurons were resuspended for a final concentration of 50,000 cells per 5 μL in Neuronal media supplemented with 1% anti-anti (GIBCO), 5% KSR ...
-
bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
-
bioRxiv - Molecular Biology 2023Quote: ... The membranes were blocked with 5% BSA and probed against P-S19-TH (#PA5-104765, ThermoFisher Scientific; 1:1000), P-S40-TH (#p1580-40 ...
-
bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
-
bioRxiv - Cell Biology 2023Quote: ... 5×107 ES cells engineered with T7-dTAG-TAF1 were fixed with 1% formaldehyde (methanol-free, Thermo Fisher Scientific) for 10 min at 25C under constant gentle rotation ...
-
bioRxiv - Biochemistry 2022Quote: ... After membrane permeabilization with 1% Triton X-100/D’PBS for 5 min cells were incubated for 30 min in the primary antibody aPMP70 (rabbit, 1:500, Invitrogen) in D’PBS supplemented with 1% BSA ...
-
bioRxiv - Cell Biology 2022Quote: ... SPD-5 and GIP-1 cDNAs was amplified by PCR and cloned into the entry vector pDONR201 (Thermo Fisher). Thereafter ...
-
bioRxiv - Cell Biology 2023Quote: ... 300-1500 ng DNA was incubated with 1-5 μl Lipofectamine 2000 in 150 μl Optimem (Thermo Fisher, 31985047) for 10 minutes at room temperature and then given to cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... On day 5 the culture medium was replaced with DMEM/F12 containing 1% N2 (v/v, Gibco, Cat#17502048), 1% GlutaMAX (v/v) ...
-
bioRxiv - Genomics 2023Quote: ... 5 μL of each restriction digest reaction was combined with 1 μL 6X TriTrack DNA Loading Dye (Thermo Fisher) and run on a 2% agarose gel pre-stained with ethidium bromide ...
-
bioRxiv - Biophysics 2023Quote: ... immunostained cells were exposed for 5 min to 1 mg/100 ml Hoechst 33342 (Thermo Fisher Scientific, Waltham, Massachusetts) in water.
-
bioRxiv - Bioengineering 2023Quote: ... in PBS for 1 hour at room temperature and subsequently blocked with 5% bovine serum albumin (ThermoFisher Scientific 37525) and 10% donkey serum (Lampire 7332100 ...
-
bioRxiv - Immunology 2023Quote: ... Confluent TeloRF cells were infected with 1 MOI UCD52 RhCMV in low serum (5%) medium in T75 flasks (ThermoFisher). Mock infection was performed in parallel ...
-
bioRxiv - Biophysics 2023Quote: ... HeLa and MDCK cells were cultured at 5% CO2 and 37°C in Dulbecco’s modified Eagle medium (DMEM, Capricorn) with 1% Penicillin Streptomycin (Gibco) and 10% fetal bovine serum (FBS ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Tissue was washed for 5 min on ice with 1× Hanks’ balanced salt solution (HBSS, Gibco, Carlsbad, CA, USA) and cut into small pieces ...
-
bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039; anti-mouse-Alexa-Fluor-647, Invitrogen A21235 ...
-
bioRxiv - Microbiology 2023Quote: ... The RNase T1 ladder was prepared by denaturing 0.4 pmol of 5′ labelled Aar with 8 µL of 1× sequencing buffer (Ambion) at 95°C for 1 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% Fetal Bovine Serum (BioWest, cat# S1620) and 1% Penicillin/Streptomycin (Fisher Scientific, cat#15-140-122) unless otherwise noted ...
-
bioRxiv - Cancer Biology 2023Quote: Approximately 1 × 107 cells were resuspended in 0.5 ml cold TNE buffer (25 mMTris-HCl, pH 7.5, 150 mM NaCl, 5 mM EDTA, and 1% Triton X-100; Surfact-Amps, ThermoFisher) with a tablet of protease inhibitors (Roche) ...
-
bioRxiv - Biophysics 2023Quote: ... About 5 µl of a 1 µg/µl sample was injected into a QExactive-Orbitrap mass spectrometer (Thermo Scientific) and a data-independent acquisition (DIA ...
-
bioRxiv - Cancer Biology 2023Quote: Single cell suspensions from dissociated larvae in 5 mL FACS tubes were stained with 1:1000 DAPI (Thermofisher, #62248) to indicate cell viability and FACS was carried out at the QMRI Flow facility using a BD 5 Laser LSR Fortessa cell analyser ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 3 nM of fluorescently labeled and biotinylated DNA (or 2x1.5 nM with experiments for the intermolecular cases) were bound to 5 nM Streptavidin in PBS and with 1:1800 Tetraspeck beads (0.1 µm, ThermoFisher). After ∼10 min incubation time ...
-
bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with secondary goat anti-rabbit antibodies (dilution 1:1000 in TBST/5% milk; Invitrogen, 32460) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... 20.000 BMDCs were co-cultured with purified T cells in a 1:5 DC:T cell ratio in sterile tissue culture medium (TCM; RPMI (GIBCO) containing 10% fetal calf serum (Bodinco) ...
-
bioRxiv - Microbiology 2024Quote: ... Confluent TRF cells were infected with 1 MOI FL-RhCMV in low serum (5%) medium in T75 flasks (ThermoFisher). Mock infection was performed in parallel ...
-
bioRxiv - Biochemistry 2024Quote: To perform DSF, 5 µM protein was mixed with substrate (0, 1, or 15 mM) and 2.5x SYPRO Orange dye (ThermoFisher), and made up to a total volume of 50 µl with DSF buffer (50 mM Tris pH 8 ...
-
bioRxiv - Genetics 2024Quote: ... Tissues were rehydrated with PBS and blocked for 1 hour at room temperature with 5% normal goat serum ((Gibco) source ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed with PBS-0.1%Tríton-X three times (5 minutes each) and incubated with the secondary antibodies (1:2000; Alexa-Fluor conjugated; Invitrogen), at RT for 1h ...
-
bioRxiv - Cancer Biology 2021Quote: ... 4mM L-Glutamine and 1X ITS (5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL selenium) (all from Gibco, Thermo Fisher Scientific, Waltham, MA). The Wit49 cell line was a gift from Dr ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
-
bioRxiv - Biochemistry 2020Quote: ... and 4 μg ml−1 Blasticidin (Thermo Fisher), single cell colonies were collected and subsequently expanded ...
-
bioRxiv - Developmental Biology 2022Quote: ... anti-Ki67 (ThermoFisher, 4-5698-82, 1:100), anti-Actinin (Cell Signaling ...
-
bioRxiv - Bioengineering 2021Quote: ... 1-2 drops of CytoSeal (Thermofisher, 8312-4) were placed on each before mounting a coverslip ...
-
bioRxiv - Bioengineering 2020Quote: ... 4’-6-diamidino-1-phenylindole (DAPI, Life Technologies) was applied at 1 μg/mL for 90 minutes to stain the nuclei and the samples were washed and mounted with AquaPoly/Mount (Polysciences) ...
-
bioRxiv - Cell Biology 2022Quote: ... ERCC 1:4 × 107 dilution (Ambion, cat: 4456740) in 384-well plates prior to library preparation.
-
bioRxiv - Genomics 2021Quote: ... 1 mL of hexane (Fisher Scientific; H303-4) followed by 1 mL of deionized water was added to each sample ...
-
bioRxiv - Bioengineering 2023Quote: ... and 4 μM Ethidum homodimer-1 (Invitrogen™) in PBS for 30 min at 37°C ...
-
bioRxiv - Genomics 2023Quote: ... 4 µL DNase I (ThermoFisher, 1 unit/µL), 4 µL RNase A (ThermoFisher ...
-
bioRxiv - Immunology 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Invitrogen) and 2-mercaptoethanol (Gibco) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... were labeled in loading buffer (HBSS with 1% FBS, 1 mM CaCl2 and 1 mM MgCl2) containing 1 μM Fluo-4 AM (Thermo Fisher) with 0.02% Pluronic F-127 at 37 °C for 30 min with gentle agitation every 10 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5 days post-transfection cells were selected with 5 μg/mL blasticidin (Thermo Fisher Scientific). When the survival rate of the cells improved ...
-
bioRxiv - Molecular Biology 2022Quote: ... 5 μl 10x Turbo DNase buffer and 5 μl 2U/μl TurboDNase (Thermo Fisher Scientific) were added to 85 μl of sample and incubated for 20-30 min at 37°C ...
-
bioRxiv - Biophysics 2021Quote: ... ×5 mm C18 PepMap 100 trap column with 5 μm particle size (Thermo Fisher Scientific) to desalt and concentrate the samples before loading onto the C18 nanocolumn for separation ...
-
bioRxiv - Biophysics 2021Quote: ... stock was prepared at 5 mg/mL in dimethyl sulfoxide (#67-68-5, Fisher Scientific) and stored at −20 °C ...
-
bioRxiv - Physiology 2020Quote: ... The 5’ end of the cDNA was further amplified using RACE (5’ RACE kit, Invitrogen) and specific primers ...
-
bioRxiv - Microbiology 2019Quote: ... 1x 10^5 cells were resuspended in water containing 5 µM propidium iodide (ThermoFisher Scientific) and incubated at room temperature for 10 min ...