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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... cells were stained for 10 min in 0.5 µL/mL calcein-AM and 2 µL/mL ethidium homodimer-1 from the Live/Dead assay kit (L3224, Invitrogen). Then ...
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bioRxiv - Developmental Biology 2023Quote: ... Stained samples were washed (3 x 5 minutes) and incubated in DAPI solution (Invitrogen, D3571; diluted 1:1000 in DPBS) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: The stained cells were washed with 1X PBS+1% FBS and centrifuged at 1200×g for 5 min twice and stained with the 1:1000 Far Red live/dead staining (Invitrogen) at room temperature for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was precipitated in 5 ml Eppendorf tubes using 1 ml of isopropanol with 3.7 μl GlycoBlue (Thermo Fisher Scientific) and incubating at −80°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended to a final concentration of 5 x 106 cells/ml and labelled with 1 µM CTV (Invitrogen) in PBS for 10 min at 37LJ°C ...
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bioRxiv - Biophysics 2024Quote: ... to which 2.5 μL of ddH2O and 2.5 μL of reverse transcription (RT) mix (1 μL 5X First strand buffer (Thermo Fisher), 0.01 M DTT ...
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bioRxiv - Immunology 2023Quote: Zebrafish larvae (6 day-post-fertilization) were incubated in embryo medium with 1% DMSO and 5 mM EdU (Invitrogen C10340) for 8 hours at 29°C after the 16 hour Vc infection ...
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bioRxiv - Biochemistry 2023Quote: ... The chamber was subsequently incubated for 5 minutes with 50 µL of 50 µg mL-1 of NeutrAvidin (Life Technologies) in casein buffer ...
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bioRxiv - Immunology 2023Quote: ... A total of 5 × 106 cells were resuspended in 500 μl of cell culture medium with 5 μg/ml of Indo-1 AM and 0.5 μg/ ml of pluronic F-127 (both from Life Technologies) and incubated in the dark for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... At 72 hours media was replaced with complete growth media containing 5 µg.mL-1 of Puromycin Dihydrochloride for selection (Gibco, A1113803). Following complete death of non-transduced cells ...
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bioRxiv - Biophysics 2023Quote: ... 300 mL NaCl, 5 mM imidazole, 10% glycerol, 0.2 mM 2-mercaptoethanol, 1 mM PMSF, and Pierce Protease Inhibitor Tablets [Thermo Fisher]). Cells were sonicated for 5 min (30% amplitude ...
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bioRxiv - Bioengineering 2023Quote: ... HeLa cells were maintained at 37 °C in a humidified incubator with 5% CO2 and passaged at 70–80% confluency using 1× TrypLE Express (Gibco).
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bioRxiv - Cell Biology 2023Quote: ... 40 cycles of 95°C for 1 s and 60°C for 20 s on the QuantStudio 5 Real-Time PCR System (ThermoFisher). Relative quantification of each gene was done by QuantStudio Design&Analysis v1.5.1 software (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then washed three times in 1x PBS for 5 minutes and countered stained with DAPI (1:20,000, Thermo Fisher). Slides were coverslipped using ProLong™ Gold Antifade Mountant (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... 15 and 21 days after the infection the media of infected and uninfected cells were incubated in the dark at 37°C/ 5% CO2/ 1% O2 with eBioscienceTM Calcein Violet 450 AM Viability Dye (Invitrogen) and Ethidium Homodimer III (Biotium ...
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bioRxiv - Bioengineering 2024Quote: ... 300 g Bloom type A gelatin which was dissolved at 15% w/v 70 °C for 1 h was diluted to 5% w/v using DMEM/F12 with HEPES (Gibco) and supplemented with CaCl2 to a final concentration of 2.5 mM and 5 U mL-1 thrombin ...
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bioRxiv - Cancer Biology 2024Quote: ... NCI-H1048 were grown in HITES medium (DMEM media supplemented with 5% FBS, 1% penicillin/streptomycin, Insulin-Transferrin-Selenium (Gibco), 10 nM Hydrocortisone (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were incubated in blocking buffer (PBS with 5% BSA) for 30 min and incubated with Alexa Fluor-568-conjugated donkey anti goat secondary antibodies (1:1000, Invitrogen) in PBS with 2.5% BSA for 1 h at RT ...
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bioRxiv - Microbiology 2023Quote: Purified DNA was used as a template for qPCR reactions by mixing 1 μl of DNA to 5 μl of 2X SYBR Select Master Mix (ThermoFisher), 3.6 μl of nuclease-free water ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked in 5 % BSA in TBS-T for 1 0.2 µg/ml Streptavidin-HRP (S911, Thermo Fisher Scientific) in 5 % BSA in TBS-T at 4 °C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2µg of DNA (LynFRB : mCherry-FKBP constructs 5:1 w/w) and 0.25µl of Lipofectamine 2000 (Thermo Fisher Scientific, 11668019), diluted in 20µl OptiMEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Neuroscience 2024Quote: ... The following day the sections were washed 3 times for 5 min each in PBS-T and incubated with the corresponding secondary antibodies (all 1:1000, Invitrogen), for 2 h at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were washed with TBST 3x for 5 min and incubated in corresponding secondary antibody for 1 hour room temperature: goat anti-rabbit antibody (Invitrogen) or rabbit anti-mouse antibody (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated neurons were plated and allowed to adhere for 2 hours at 37°C in 5% CO2 wells were flooded with DMEM/F12 plus GlutaMAX culture media containing 1% penicillin/streptomycin (15070063, Thermofisher), 10 ng/mL mouse nerve growth factor (01-125 ...
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bioRxiv - Physiology 2024Quote: Mouse pancreata or human and mouse islet sections were then washed with PBS for 5 minutes and permeabilized and blocked with 1% normal donkey serum and 0.15% Triton X-100 (Fisher Scientific) in PBS for 1 hour ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... cortical neurons were resuspended in a plating medium (5% FBS, insulin, glutamate, G5 and 1 × B27) supplemented with 100 × L-glutamine in Neurobasal medium (Invitrogen) and plated at a density of 100,000 cells per cm2 on polyornithine- and fibronectin-coated coverslips (Carolina Cover Glasses ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed in PBS three times (5 mins) and incubated in the secondary antibodies Alexa Fluor donkey anti goat 488 (1:500, cat# A-11055, Invitrogen), diluted in blocking solution for two hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were washed three times for 5 minutes in PBS and incubated for 1 hour in blocking solution [10% normal goat serum (NGS; #50197Z, Invitrogen), 0.1% Triton X-100 in PBS] ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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bioRxiv - Bioengineering 2024Quote: ... and splenocyte to resuspend post centrifugation for 5 minutes and subsequently diluted with 5mL ice-cold FACS Buffer (2 or 10% FBS in 1× HBSS (Gibco) or 1X PBS ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... using standards covering a range from 10-1 to 10-5 ng/µl fungal DNA (measured using a NanoDrop spectrophotometer, Thermofisher). Each run included the standards and a negative control ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were incubated with secondary antibodies diluted 1:1000 in 5% BSA in PBST (anti-mouse Alexa Fluor 555, CST 4409S; anti-mouse Alexa Fluor 647, Invitrogen, A-21235 ...
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bioRxiv - Developmental Biology 2024Quote: ... 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’) and 1 μl of 10 mM dNTP mix (Thermo Fisher). Illumina libraries were prepared by using a modified smart-seq2 protocol [Picelli 2014] using SuperScript IV RT and tagmentation procedure as previously described [Henning 2018] ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were visualized by electrophoresis on 0.5% Tris-acetate-EDTA (TAE) agarose gel at 100 V for 1 h using SYBR Safe DNA Gel Stain (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Fisher) solution ...
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bioRxiv - Biochemistry 2024Quote: ... and HFF-1 cells were maintained at 37°C under 5% CO2 atmosphere in RPMI-1640 medium (ThermoFisher, for HeLa) and DMEM (ThermoFisher ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and (MtWOX5) 5’-CACCATGCAGACGGTCCGAGATCTGTC-3’ and 5’- CCTTCGCTTAAGTTTCATGTAA-3’ (AhWOX5) and cloned into pENTR-dTOPO (Invitrogen). The entry clones of AhWOX5 & MtWOX5 recombined through LR clonase Gateway technology (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were selected 5 days after the transfection with 5 µg/mL blasticidin (Thermo Fisher Scientific). They were then seeded at low density and single cell colonies were picked and genotyped by PCR ...
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bioRxiv - Physiology 2020Quote: ... Eyes were decontaminated by submersion in 5% povidone-iodine ophthalmic solution (Betadine 5%, Fisher Scientific, NC9771653) for two minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... the PCR products obtained with 2 primers (AV48_SMCHD1_gPCR_F: 5’-AGGAGCGCGTTTGAATCGG-3’, AV47_SMCHD1_gPCR_R 5’-CTTCGCGTACCTGACACACAC-3’) were TOPO-cloned (Thermo Fisher, #450071) and sent for sequencing.
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bioRxiv - Genomics 2019Quote: ... after which cells were frozen at 5 × 106 cells/mL in 5% DMSO (Thermo Fisher Scientific). For the MPRA ...
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bioRxiv - Microbiology 2020Quote: ... 5% CO2 and 5% O2 at 37°C in RPMI 1640 containing AlbuMAXII (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2022Quote: ... C18 trap (300 μm i.d x 5 mm, C18 PepMap100, 5 μm, 100 Å; Thermo Scientific) analytical columns (Acclaim PepMap TM RSLC ...
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bioRxiv - Cell Biology 2023Quote: ... Target mtDNA gene (Forward primer: 5′-CACCCAAGAACAGGGTTTGT-3′, Reverse primer: 5′-TGGCCATGGGTATGTTGTTAA-3′, Invitrogen custom primers) and reference 18S ribosomal RNA gene (Forward primer ...
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bioRxiv - Synthetic Biology 2023Quote: ... URA3 containing CRISPR plasmids were removed using the 5-Fluoroorotic Acid (5-FOA) (Thermo Fisher Scientific) counter-selection method with a synthetic defined medium supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... 5-fold serial dilutions of serum (assay diluent: PBS + 5% goat serum [Gibco] + 0.05% Tween-20) was added and incubated for 2 hours at 37°C (IgG ...
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bioRxiv - Genetics 2023Quote: ... non-specific sites were blocked with PBS/5% FBS/5% NGS (normal goat serum, Life technologies) for 1 h at RT ...
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bioRxiv - Biochemistry 2024Quote: ... blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher) at 95% humidity and 4 °C ...