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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 5 μl of the Phire PCR reaction was verified on a 1% agarose gel (Ultra-Pure agarose, # 16500-500, Invitrogen), with a 1kb bench top ladder (G754A ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... Samples were diluted 1:5 in sample loading buffer and quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific). Lysates were normalized to 100μg/mL in 1X Bolt™ LDS Sample Buffer (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Samples were then blocked with 5% FBS in PBS for 20 min before stained with Alexa Flour 594 phalloidin (1:100, Invitrogen) for 1 h and 10 μg/ml DAPI (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Normal adult donor blood (in EDTA) was diluted 1:5 with RPMI 1640 supplemented with pen/strep and 2mM L-glutamine (Gibco). Cells were incubated at 37°C for 2 hours in the presence of polymyxin B (1.25μg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... iPSCs were split with EDTA at 1:5 ratios in culture dishes coated with matrigel and culture in N2B27 medium (comprised of DMEM/F12 medium (Invitrogen) supplemented with 1% MEM-nonessential amino acids (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... 1 ng-5 μg of RNA per reaction was used in SuperScript™ First-Strand synthesis kits (Invitrogen, ID: 11904018). Nucleic acid purity was assessed using a NanoPhotometer® (Implen).
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bioRxiv - Neuroscience 2022Quote: ... 5 mM EDTA and 1% BSA in HBSS) and 2μL of 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific, A1310) at a concentration of 0.1 mg/mL were added ...
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bioRxiv - Synthetic Biology 2022Quote: ... A 1:10 dilution was performed and 2μl of diluted DNA was amplified by mean of a QuantStudio 5 (Applied Biosystems) thermal cycler using the SYgreen Blue Mix (PCRbio ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Microbiology 2023Quote: ... any contaminating DNA was removed by treating 1-5 μg of RNA with the TURBO DNase enzyme kit and purified according to the manufacturer’s instructions (ThermoFisher Scientific). For mouse bladder RNA samples ...
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bioRxiv - Microbiology 2023Quote: ... 1 μL of dNTPs (10 mM) and 0.5 μL of Platinum Taq DNA polymerase (5 U/ml) (Invitrogen, Marseille, France) and 1 μL of forward and reverse primer (20 μM) ...
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bioRxiv - Microbiology 2023Quote: ... The infection was incubated for 2-3 hours at 37°C and then the viral inoculum was removed and replaced with a pH 7.2 methyl cellulose overlay medium (1% methyl cellulose [Sigma-Aldrich, MA]/5% FBS [Gibco, MA]/1X penicillin-streptomycin [Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The infection was incubated for 2-3 hours at 37°C and then the viral inoculum was removed and replaced with a pH 7.2 methyl cellulose overlay medium (1% methyl cellulose [Sigma-Aldrich, MA]/5% FBS [Gibco, MA]/1X penicillin-streptomycin [Gibco, MA]/44 mM sodium bicarbonate [Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... The cells were spun down at 1,200 xg for 5 minutes and washed with 1X PBS+ 1% FBS once before staining with Aqua Live/Dead stain (Invitrogen) at room temperature for 25 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 2.5 µg of RNA was treated with 1 unit of Turbo DNase using the TURBO DNA-free Kit (AM1907, Invitrogen) and incubated for 30 min at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... The concentrated virus was diluted 1:5 in hepatocyte medium containing N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid buffer (HEPES; 20 mM; Gibco) and 4 μm/mL polybrene (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to the culture medium (0.5 mM) for the final 1 h of drug/vehicle treatment and cellular RNA was purified using Trizol reagent (ThermoFisher). Newly synthesized ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific]; 5 M NaCl [#01094764, ThermoFisher Scientific]; 90 mM KCl [#01276444, ThermoFisher Scientific] ...
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bioRxiv - Cell Biology 2023Quote: ... cells were then treated for 5 min at RT with Hoechst 33342 (1 µg/mL in PBS buffer, Invitrogen, H1399), followed by two additional washes of 10 min each with PBS buffer ...
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bioRxiv - Cell Biology 2023Quote: ... the iMACs were washed 3 x 5 min with PBS and incubated for 1h with 1:1000 goat anti-mouse IgG-AF488 (A11001, Invitrogen). Afterwards the cells were again washed 3 x 5 min with PBS ...
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bioRxiv - Biophysics 2023Quote: ... the tissue was incubated for 5 min with 1 µM TO-PRO-3 iodide (Invitrogen, Life Technologies Corporation, Oregon, USA) followed by washing with 1x DPBS (nuclei data not shown) ...
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bioRxiv - Cell Biology 2023Quote: ... REF52 cells were grown in six-well plates to 60% confluency and transfected with 1µg of the vector together with 1 µl of Lipofectamine 3000 and 5 µl of P3000 solution (ThermoFisher Scientific). Two days post transfection cells were detached ...
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bioRxiv - Cancer Biology 2023Quote: ... 5% non-fat milk powder) and then incubated with primary antibody against ERα (ThermoFisher #MA5-14104, 1:1000 in BS), EHMT2/G9a (Abcam #ab133482 ...
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bioRxiv - Cancer Biology 2023Quote: ... Horseradish-peroxidase conjugated secondary antibodies diluted 1:5000 in 5% non-fat milk were used and visualized with SuperSignal West Pico or Femto substrate kits (ThermoFisher). The following commercial primary antibodies supplemented with 0.02% sodium azide were used for immunoblot analysis ...
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bioRxiv - Neuroscience 2023Quote: ... The total RNA concentration was detected using a Thermo Scientific MultiskanGO Microplate Spectrophotometer and 1 μg was immediately retro-transcribed using a mixture containing: 5× first strand buffer (18080-044, Invitrogen,), 2.5 μM of random hexamers (N112470 ...
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bioRxiv - Neuroscience 2023Quote: ... or DIV12 (overexpression) primary hippocampal neurons were transfected with 1-2 μg plasmid DNA and 5 μL Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The cells were spun down at 1200×g for 5 minutes and washed with 1X PBS+ 1% FBS once before staining with Aqua Live/Dead stain (Invitrogen) at room temperature for 25 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed brains were blocked in 5% NGS then incubated overnight with the primary antibodies anti-GFP (1:100, Invitrogen: G10362), anti-Fas2 [1:25 ...
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bioRxiv - Microbiology 2023Quote: ... and immortalised HFF cells (ATCC CRL-4001) were grown at 37°C with 5% CO2 in DMEM medium supplemented with 10% FBS and 1% Glutamax (Gibco). Gold grids with SiO2 film (Quantifoil ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were subjected to selection media (DMEM-GlutaMax plus 5% FBS) containing 400 μg mL−1 of Geneticin (Invitrogen). The growth of single cell-derived colonies was monitored over two weeks ...
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bioRxiv - Pathology 2023Quote: ... 24 hours after plating medium was replaced by Basal medium supplemented with 5 ng/ml EGF and 1/100 dilution of Insulin-Transferrin-Sodium Selenite (ITS) Supplement (Invitrogen) and 3 % of fresh Matrigel ...
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bioRxiv - Genomics 2023Quote: ... 0.5-1 µg of RNA was reverse-transcribed with random hexamer primers using SuperScript III reverse transcriptase (18080-051; Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... slices containing the dorsal hippocampus were washed for 5 mins with 1X PBS three times before staining the slices for 10 minutes with DAPI (1:1,000, Life Technologies) and mounted on slides with Vectashield (Vector Labs) ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and the nuclei pellet was resuspended in 2 mL of ice-cold resuspension buffer (1 M HEPES [#J16924- AE, ThermoFisher Scientific]; 5 M NaCl [#01094764, ThermoFisher Scientific] ...
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bioRxiv - Bioengineering 2023Quote: ... from IDT and 2.5µL of the diluted cDNA (1:5 dilution) in a 10µL reaction with Fast SYBR Green Master Mix (Life Technologies) and a LightCycler® 480 Real-time PCR System (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... the buffer was exchanged to HBS buffer (1×HBS pH 7.2, 5% (v/v) glycerol) by gel filtration (Zeba Spin Desalting column, 2 mL, Thermo Fisher). For immunoblotting ...
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bioRxiv - Biochemistry 2022Quote: ... Tissue culture was performed at 37°C in a 5 % CO2 humidified incubator using 1 ml of DMEM media supplemented with 20 % FBS (GIBCO), 100 U/ml penicillin (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed 3 times with 5% milk TBST and incubated with HRP-conjugated secondary antibodies (1:5000, 32230/32260; Invitrogen). Data were visualized using chemiluminescence detection on ChemiDoc Touch (Bio-Rad Laboratories).
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bioRxiv - Genetics 2023Quote: ... The nuclear extract was denatured at 95-100°C for 5 minutes in 1× NuPAGE LDS sample buffer (ThermoFisher, NP0007) containing 10% v/v β-Mercaptoethanol (Millipore Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated for 5 minutes with 1 ml of Supersignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) and imaged using the ‘chemi high-resolution’ setting on a Bio-Rad ChemiDoc MP System.
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bioRxiv - Molecular Biology 2023Quote: ... Blots were incubated for 5 minutes with 1 ml of Supersignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34095) and the final blot were imaged using the ‘chemi high-resolution’ setting on a Bio-Rad ChemiDoc MP System.
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with PBS and incubated with 1 µg/mL Hoechst 33342 (Thermo) and 5 μg/mL Wheat Germ Agglutinin Oregon Green 488 Conjugate (Invitrogen) for 10 min at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed again two times in 1XPBS 5’ each and incubated with Alexa Fluor Tyramide 594 (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... washed three times in 1XPBS for 5’ each and incubated for 10’ at RT with Hoechst (1:1000, in 1XPBS, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... were enzymatically and mechanically generated and cultured in suspension in the presence of BMP4 (1 ng/mL) and ROCK inhibitor (5 µM) for 24 hours in StemPro-34 media (Gibco) supplemented with L-glutamine (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... at RT for 10 min and blocked at RT for 1 hr in blocking solution (5% NBCS, 2x antibiotic-antimycotic (Gibco), 0.1% sodium azide (Sigma s2002-100G) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed again in 0.1% PBS-Triton and the nucleic acids were labeled with 1 µg/mL DAPI for 5 minutes before cells were mounted on a slide with ProlongGold (ThermoFisher). Fluorescence images were taken using a confocal fluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin] in a 37 °C incubator with 5% CO2 [all reagents were purchased from Gibco, Thermo Fisher Scientific (Waltham ...
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bioRxiv - Bioengineering 2023Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...