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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... a mix containing a 5:1 molar ratio of the pA-uL11K3A-HA expression vector and the selection plasmid pCoBlast (Invitrogen) was prepared ...
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was centrifuged at 200 g for 5 minutes and the resulting pellet was resuspended in 1 mL of DMEM (Gibco) supplemented with 10% FBS and 1% antibiotic-antimycotic (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... Then slides were washed 3×5’ in TBS and incubated in donkey-α-sheep-488 (1:500, Life Technologies, A11015) in TBS for 90’ ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... siRNA reverse transfection was performed by mixing 1.2 µl siRNA 5 µM with 1 µl lipofectamine RNAiMAX transfection reagent (Invitrogen, ThermoFisher Scientific) to a final volume of 100 µl with Opti-MEM® (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... were generated from H9 hESCs and cultured in suspension in the presence of BMP4 (1 ng/mL) and ROCK inhibitor (5 uM) for 24 hours in StemPro-34 medium (Gibco) supplemented with L-glutamine (Gibco) ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Immunology 2022Quote: ... albicans hyphae at roughly MOI 5 were co-incubated with Sytox green (final concentration 1 μM) (Life Technologies, Eugene, OR) for 5 hours at 37°C with 5% CO2 ...
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bioRxiv - Plant Biology 2019Quote: ... and appropriate buffer containing deoxynucleoside triphosphates (dNTPs) and MgCl2 in a final concentration of 5 mM (1×; Thermo Fisher Scientific), was incubated at 25°C for 10 min and at 37°C for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... was diluted 1:200 in PBS/5% FCS/0.1% Tween® 20 and primary MMP1 antibody (mouse anti-human; Invitrogen Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Microbiology 2021Quote: ... The cells were left to fix for 1 h at room temperature before transferring 5 μl into 15 μl ProLongTM Gold Antifade mountant (ThermoFisher). For cell membrane staining ...
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bioRxiv - Molecular Biology 2021Quote: To label HELB with QDs we incubated a 1:5 molar ratio solution of biotinylated HELB and streptavidin-coated QDs 525 (Q10143, Invitrogen) for 30 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS and incubated for 1 h at 37°C and 5% CO2 with Opti-MEM (Gibco) containing the indicated inhibitors ...
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bioRxiv - Cancer Biology 2019Quote: ... Input for each sample consisted of 500 ng of RNA and 5 μL of 1:500 diluted ERCC spike-in mix 2 (Ambion). Libraries were amplified for 11 cycles during the final amplification step ...
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bioRxiv - Biochemistry 2020Quote: ... charged by Ni2+ ions or 1 ml Tricorn 5/10 column (Cytiva) packed by Ni-NTA resin (Thermo Fisher Scientific). Columns were equilibrated by the 50 mM Na-PO4 ...
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bioRxiv - Molecular Biology 2019Quote: ... The percentage of CD4-expressing cells was measured after incubation for 10 minutes with 1 µl of anti-CD4 antibody coupled to Alexa 647 (rat isotype, RM4-5, Invitrogen). For each cell line ...
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bioRxiv - Biochemistry 2019Quote: ... blocked with 5% milk in TBST and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (ThermoFisher MA1-21315, 1:3000). Blots were incubated with primary antibodies overnight at 4 °C with rocking and were then washed (3×5min ...
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bioRxiv - Neuroscience 2019Quote: ... centrifuged for 5 minutes at 400 × g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin) ...
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bioRxiv - Microbiology 2019Quote: Images were captured using an EVOS XL Core (Fig 1) or EVOS FL (Fig 5) Cell Imaging System (ThermoFisher Scientific). To measure transfection efficiency ...
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bioRxiv - Genetics 2020Quote: ... centrifuged for 5 minutes at 400g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin Y27632 (10 µM) ...
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bioRxiv - Immunology 2021Quote: ... radius and ulna dependent on the cell numbers required and collected into PBS/5% (v/v) FBS/1% (v/v) Penicillin/Streptomycin (ThermoFisher). All subsequent processing was undertaken in a biological safety level 2 tissue culture hood with sterile reagents ...
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bioRxiv - Microbiology 2021Quote: ... or hydrogen peroxide (5 and 10 mM) and incubated with or without 50 μM of diphenyl-1-pyrenylphosphine (DPPP) (ThermoFisher) at 37°C (68 ...
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bioRxiv - Cell Biology 2021Quote: ... and the suspension diluted 1/5 by adding FACS buffer (FB; 2% heat-inactivated Fetal bovine serum (Life Technologies; 26140079), 25 mM HEPES (Life Technologies ...
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bioRxiv - Physiology 2021Quote: ... Plates were incubated for 1 h at 37°C with 5% CO2 and were then overlaid with 1X MEM (Gibco) containing 2% FBS and 0.6% Avicel for 2 days ...
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bioRxiv - Biochemistry 2021Quote: ... and 300 mM NaCl] was incubated with 20-fold excess (1 mM final concentration) of tetramethylrhodamine-5-maleimide (TMR) (Invitrogen) at room temperature in the dark for 2–4 h ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were dried for 20 min at room temperature then resuspended in 5 μL of 1 M Tris and 10 μL of protein loading buffer (2x NuPAGE LDS sample buffer (Invitrogen), 715 mM 2-mercaptoethanol) ...
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bioRxiv - Genomics 2022Quote: ... THP-1 cells were separately transduced with lentivirus expressing sgRNA targeting each candidate gene and selected with 5 μg mL-1 Puromycin (A1113802, Gibco) for 7 days ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D131-#6) were labeled by 15 min incubation with 1 μM CFSE (CellTrace CFSE, ThermoFisher Scientific, Ref: C34554). Cells were then resuspended in PBS supplemented with 30% Percoll ...
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bioRxiv - Cell Biology 2020Quote: ... with 10% FBS and 1% pen/strep and MCF10A cells were cultured in DMEM/F-12 supplemented with 5% horse serum (Gibco), 20ng/ml epidermal growth factor ...
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bioRxiv - Cell Biology 2021Quote: Whole fly RNA was prepared from mixed sex samples (5-15 flies per sample, flies < 1 week old) with Trizol reagent (Invitrogen) according the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... 0.5 mM EDTA; 0.5 mM EGTA; 1% Triton X-100; 0.5 mM PMSF; and Protease Inhibitor Cocktail [Thermo Fisher Scientific]). Protein concentration was measured using a BCA protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... DNA was stained for 5 minutes in PBS with 1 ug/ml of DAPI and coverslips mounted with Pro-Long Gold (Invitrogen). Imaging was performed using a Leica SPE2 confocal microscope using LAS X version 3.3.0 software (Leica Microsystems ...
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bioRxiv - Immunology 2021Quote: ... The lysis was performed in PCR strips by transferring 5 µl of worm pellet into 45 µl of Lysis Solution supplemented with 1:100 DNase I (Ambion). The samples were then lysed by at least 10 freeze-thaw cycles using liquid nitrogen and a warm water bath ...
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bioRxiv - Genetics 2020Quote: ... Real-time quantitative PCR were performed on 1ml of diluted RT reactions (1/5) using SYBR Green kit (Life Technologies) on a One Step Plus thermocycler (Thermo Fisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then incubated in the blocking/0.5 % BSA solution containing 1:500 of a secondary antibody (goat anti-chicken, Alexa Fluor 488, Invitrogen, # A-11039; ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: Mutagenized Aβ was then ligated into the linearized plasmid in a 5:1 ratio (insert:vector) using a ligase treatment (T4, Thermo Scientific) overnight ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 1-2×106/mL in 50-100 µL of cold HBSS containing 5% heat-inactivated FBS (Gibco), 100U/mL penicillin ...
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bioRxiv - Molecular Biology 2022Quote: ... and a goat anti-rabbit secondary antibody (1:3000, SeraCare, Milford, MA) in 5% bovine serum albumin (BSA, Thermo Fisher) in TBST ...
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bioRxiv - Biochemistry 2022Quote: ... which was hydrolyzed by adding 1.5 μL of 10 units/μL T4 PNK (polynucleotide kinase) and 1 μL RNase-Out solution (40 units/μL, ThermoFisher) to the cleavage reaction above ...
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bioRxiv - Bioengineering 2022Quote: ... Next donkey-anti mouse Alexa Fluor 647 was added to the medium to a final concentration of 1:1000 and incubated on ice for 5 min (Invitrogen). 200 μL of the medium was then introduced to the cells cultured in a well of a 96 well-plate and incubated for 5 min at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3x for 5 min with TTBS and incubated 1 h at RT under agitation with anti-GFP-HRP (Thermofisher) diluted 2,500 fold in 1% BSA in TTBS ...
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bioRxiv - Developmental Biology 2022Quote: Embryos/tadpoles were either injected intra-abdominally (at stage < 41) or bathed (at later stages) with/in a 1 mM 5-ethynyl-20-deoxyuridine solution (EdU, Invitrogen). They were then fixed after the desired time-period in 4% paraformaldehyde ...
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bioRxiv - Genetics 2022Quote: ... Membranes were blocked with 5% nonfat milk and probed with mouse anti-FLAG (MA1-91878; Invitrogen; 1:1000; RRID AB_1957945), rabbit anti-GFP (NB600-308 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cells were split with EDTA at 1:5 ratios in culture dishes coated with matrigel and culture in N2B27 medium (comprised of DMEM/F12 medium (Invitrogen) supplemented with 1% MEM-nonessential amino acids (Invitrogen) ...
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bioRxiv - Genomics 2022Quote: ZIP13K2-derived EN cells (5 x 106 /IP) were harvested and cross-linked in 1% formaldehyde (Thermo Fisher Scientific, 28908) in DPBS for 10 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... and the vesicular stomatitis virus G (VSV-G)-expressing plasmid at a ratio of 7:7:1 (5 μg total amount of DNA) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Neuroscience 2022Quote: Real-time PCR was performed with a 1:5 dilution of cDNA using QuantStudio3 real-time PCR apparatus (Life Technologies) and TaqMan® reagents with predesigned assays for the target genes GAD65 (alias GAD2 ...