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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were quantified with Pierce™ BCA Protein Assay Kit (Life Technologies) and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant T-REx-HEK-CFTR cells were generated by lipofectamine transfection (Life Technologies) of plasmids containing mutated versions of CFTR (RefSeq NM_000492) ...
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bioRxiv - Biochemistry 2023Quote: ... 6 µg RNA was used and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-H3K4me1 (recombinant polyclonal antibody, ChIP-Verified, 71095 ThermoFisher Scientific, Invitrogen, 1:100) and H3K27ac (Acetyl-Histone H3 (Lys27 ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant chimeric antibodies were produced using the Expi293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human full-length bFGF (PHG0261, Gibco; Thermo Fisher Scientific, MA, USA) at 50 ng/ml24.
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were produced in suspension culture in Freestyle 293F cells (Life Technologies) transiently transfected using FectroPro reagent (Polyplus) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... all iPSCs were cultured in Vitronectin Recombinant Human Protein (VTN-N) (ThermoFisher, A14700) pre-coated 6-well plates with 2 mL TeSR-E8 medium (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Recombinant TEVp was purified as previously described1 or was purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... were coated with 50 ng of recombinant Gag/well (35) in 1xPBS (Gibco) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The recombinant NS1 gene was cloned into the pTRC–His2c expression vector (Invitrogen) and expressed in the BirA-enzyme containing E ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with PBS and incubated with recombinant enzymes TrypLE (Thermo Fisher) for 10 minutes at 37°C for single cell dissociation ...
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bioRxiv - Neuroscience 2024Quote: For recombinant lentivirus production - HEK293FT cell line (cat# R70007, ThermoFisher Scientific, Auckland, NZ), HT1080 cell line (cat# CCL-121 ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Primary Strep-MAb classic chromeo-555 (IBA) and secondary Alexa-fluor-488 or −555 goat anti-mouse (Invitrogen) were applied sequentially with PBS washes in between ...
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bioRxiv - Bioengineering 2021Quote: ... cells were pre-incubated with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 µL of PBS with 2% FCS for 15 minutes at 4 °C ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Briefly, sheep-anti mouse IgG paramagnetic beads (Dynal, 11201D) were coupled with anti-His mAb (Invitrogen, 37-2900) in coating buffer (PBS plus 0.1% immunoglobulin-free BSA ...
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bioRxiv - Immunology 2021Quote: ... or with unconjugated 4.3.1.3 mouse MAb and secondary detection with goat anti-mouse Alexa-Fluor 647 (A-21235; Life Technologies). Immunofluorescence was used to boost GFP signal in TgBAC(lck:gfp)vcc6 specimens with a chicken anti-GFP primary (Abcam ...
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bioRxiv - Biophysics 2022Quote: ... phagosomes were washed 3X with MAB and incubated with secondary antibodies against mouse (Alexa488; A11029, Thermo Fisher Scientific) and rabbit (Alexa647 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The bound mAb was detected with adding 100 μl/well of 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Life Technologies) and incubation at room temperature for 5 min prior to the addition of 100 μl of 3% H2SO4 to stop the reaction ...
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bioRxiv - Microbiology 2021Quote: ... The isotyping of the selected clones was performed using the commercial Rapid ELISA Mouse mAb Isotyping kit (ThermoFisher). The kit determines the presence of the murine isotypes IgG1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... we obtained the sequence information of the 14.4.4 mAb VH and VL chain by isolating total mRNA from a 14.4.4 mAb-producing hybridoma cell line with the use of TRIzol (Invitrogen) followed by reversely transcribing the isolated mRNA into cDNA (First-strand cDNA Kit ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated with mouse anti-human E-cad ectodomain mAb (1:5000) (HECD-131700, Invitrogen, USA) for 2 h ...
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bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed on 50-µm sections with the anti-pTau mAb AT8 (ThermoFisher) plus the anti-mouse IgG H + L secondary antibody conjugated with Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD8+ T cells were stimulated with αCD3 and αCD28 mAb-coated dynabeads (Thermo Fisher Scientific, no. 11452D).
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bioRxiv - Microbiology 2024Quote: ... mAb pool was dialyzed in PBS pH 7.4 using Slide-A-Lyzer G2 Dialysis Cassette 3.5K (Thermo Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... His tag) (BLPSN-0986P, BETA Life Sciences, Fairfield, NJ, USA) was immobilized on a 96-well plate (442404, MAXISORP F96 NUNC Immuno-plate ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were then incubated for an addition 12 – 24 hrs with 1:400 mouse anti-beta III tubulin (Invitrogen, 480011), 1:300 sheep anti-ChAT (Abcam ...
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bioRxiv - Microbiology 2020Quote: Luminex profiling was performed on whole blood that was allowed to clot for 20 minutes and then spun down using a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Pathology 2019Quote: The primary antibody used for immunofluorescent analysis in rehydrated sections included: beta-amyloid (36-6900; 1:100, Thermo Fisher Scientific); αB-crystallin (Ab151722 ...
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bioRxiv - Cancer Biology 2020Quote: ... The relative expression of each target gene represents an average of triplicates that are normalized to the transcription levels of beta-actin (Hs99999903_m1; ThermoFisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Beta-lactamase activity was determined at 390 nm (yellow) and 486 nm (red) using a VarioSkan plate reader (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Pathology 2019Quote: ... Membranes were blocked with 2.5% BSA and incubated with primary antibodies: beta-amyloid (36-6900; 1:100, Thermo Fisher Scientific); anti-ZO-1 (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The first injection contained either vehicle (0.9% saline) or the 11-beta hydroxylase inhibitor metyrapone (20 mg/kg) (Fisher Scientific) in saline solution ...
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bioRxiv - Immunology 2021Quote: The RBD was conjugated to CuMVTT using the cross-linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, MA) at 10-molar excess for 60 minutes at 23°C ...
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bioRxiv - Neuroscience 2022Quote: ... media was changed to KSR media (Knockout DMEM, 15% KOSR, 1x MEM-NEAA, 55 μM beta-mercaptoethanol, 1x GlutaMAX; Gibco) with doxycycline (2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Serial sections were processed for immunohistochemistry using the 6F/3D mouse monoclonal amyloid-beta antibody (1:50; ThermoFisher, Waltham, MA) to visualize amyloid plaque pathology.
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bioRxiv - Bioengineering 2022Quote: ... mouse INFB1 (FAM, Mm00439552_s1) and Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited) (Thermofisher) and Applied biosystems Taqman™ Fast Universal PCR Master Mix (2x ...
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bioRxiv - Microbiology 2023Quote: ... containing a final concentration of 2.5% beta-mercaptoethanol and loaded onto Bolt 4-12% Bis-Tris gels (ThermoFisher Scientific; NW0412) at equivalent concentrations (15-20 µg for cFLIP knockout validation based on available lysates ...
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bioRxiv - Microbiology 2023Quote: ... NP and 250 ng of pPolI plasmid encoding a mvRNA were transfected alongside 100 ng of plasmid expressing firefly luciferase from the IFN-beta promoter and 10 ng of plasmid expressing Renilla luciferase using Lipofectamine 2000 (Invitrogen) to the manufacturer’s specifications ...