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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were cultured on human recombinant vitronectin in StemFlexTM media (Life Technologies) and differentiation was initiated by plating singularised hiPSCs on human embryonic stem cells (hESC)-qualified Matrigel (Corning ...
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bioRxiv - Biophysics 2024Quote: ... Recombinant CtNAT10 protein was expressed in both Sf9 cells (ThermoFisher, cat #12659017) and homemade bGroESL cells (See Experimental Model and Subject Details) ...
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bioRxiv - Cell Biology 2024Quote: ... 20 % FBS and 200 ng/mL Human M-CSF Recombinant Protein (ThermoFisher). Six days after the initial isolation ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Escherichia coli BL21(DE3) pLysS (Invitrogen) transformed with respective plasmids ...
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bioRxiv - Neuroscience 2019Quote: ... was then applied for 2 hours along with an Alexa Flour 546 anti rabbit antibody (Life technologies, 1:500). After five washes ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... before 2-h incubation at room temperature with a secondary antibody Alexa 555 made in rabbit (Invitrogen, 1:500). After several washes ...
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bioRxiv - Immunology 2020Quote: ... PE-conjugated F(ab’)2-goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody was purchased from Invitrogen.
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bioRxiv - Immunology 2021Quote: ... and biotinylated anti-Ym1/2 (polyclonal, R and D Systems Cat# BAF2446, RRID:AB_2260451) followed by Zenon anti–rabbit reagent (Thermo Fisher Scientific Cat# Z25302 ...
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bioRxiv - Immunology 2021Quote: ... The sections were then incubated for 2 hours at room temperature using: rabbit polyclonal antibody-1 for myeloperoxidase (Invitrogen) to examine neutrophils ...
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bioRxiv - Genomics 2021Quote: ... and incubated with AbDil containing 2 ug/ml Alexa 647 fluorophore conjugated goat anti-rabbit secondary antibody (Molecular Probes) for 30 minutes ...
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bioRxiv - Biophysics 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit Alexa 647, Invitrogen, A-27040 ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 1:5000 dilution of Alexa Fluor 488 labeled goat anti-rabbit IgG 2° Ab (Invitrogen, A-11070) with blocking buffer and incubated for 30 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142, Thermo Fisher, France) and revealed using an anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in goat anti-rabbit Alexa Fluor 568 secondary antibody (1:200 in 2% BSA and 0.1% Triton; Invitrogen) overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The Alexa Fluor 647 rabbit@goat secondary antibody (2 µg/mL in BB, #A-21446, Invitrogen, Thermo Scientific, Germany) was added to the cells and incubated for 1 h at room temperature with gentle shaking ...
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bioRxiv - Biophysics 2023Quote: ... The Alexa Fluor 647 rabbit@goat secondary antibody (2 µg/mL in BB, #A-21446, Invitrogen, Thermo Scientific, Germany) was added to the cells and incubated for 1 h at room temperature with gentle shaking ...
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bioRxiv - Microbiology 2024Quote: ... rabbit (goat anti-rabbit IgG, Invitrogen), sheep (rabbit anti-sheep IgG ...
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bioRxiv - Neuroscience 2020Quote: ... anti-IFN-γ neutralizing mAb and/or an isotype IgG1 (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) were intraperitoneally injected daily to mice from PND5 to PND10 ...
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bioRxiv - Microbiology 2022Quote: ... The V5 epitope was detected with mouse mAb anti-V5 (diluted 1:1000) (Invitrogen, catalong no. R96025). Toxoplasma-specific antibodies include mouse mAb m-IMC1 (diluted 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... anti-Utr2 mAbs or a commercially sourced anti-Candida mouse IgG2a monoclonal antibody (MA1-7009) (Fisher Scientific) in pre-warmed supplemented CO2-independent medium and incubated at 37 °C with gentle shaking for 40 min.
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bioRxiv - Biochemistry 2021Quote: ... The endoplasmic reticulum was stained using an Alexa488-coupled anti-calnexin (1:200, mAb AF18, ThermoFisher Scientific) in 10% goat serum overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of the purified mAb was determined using the Coomassie Plus protein assay reagent (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Oregon green 488 was first conjugated to mAb 18B7 using Oregon Green 488 Protein Labeling Kit (ThermoFisher). BMDMs were plated at a density of 1.25 × 105 cells/well on 24-well plate with 12 mm circular coverslip ...
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bioRxiv - Immunology 2021Quote: ... The following primary antibodies (Ab) were used: occludin (mouse monoclonal [mAb], clone OC-3F10, Thermo Fisher Scientific), TFF3 (rabbit polyclonal [pAb] ...
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bioRxiv - Microbiology 2023Quote: ... Blots were then incubated (1:500 dilution) with mouse anti-human E-cad mAb (4A2C7, Life Technologies) and anti-human HtrA (PA523395 ...
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bioRxiv - Microbiology 2023Quote: ... they were stained with a mastermix of anti-cytokine/transcription factor mAbs containing BCL6 PE (ThermoFisher Scientific) at 4 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: The following primary antibodies were used: anti-ezrin mouse monoclonal antibody (mAb) (1:500; MA5-13862, Invitrogen); anti-p75 NTR rabbit mAb (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were produced at MAB Discovery GmbH using the FreeStyle™ 293 expression system from Thermo Fisher. Transfected HEK-293 FreeStyle™ cells were incubated for 10-11 days at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the TCO- or Tz-conjugated mAbs were then labeled with Alexa Fluor 488 (Thermo Fisher Scientific, A2042), Alexa Fluor 594 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The assay for MUC5AC was performed using biotin-conjugated mouse anti-MUC5AC mAb (45M1, Invitrogen, #MA5-12175) as described previously (12) ...
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bioRxiv - Biophysics 2024Quote: ... The 3 mAbs were specifically conjugated to HRP using EZ-Link™ Plus Activated Peroxidase Kit (ThermoFisher) and detected with ECL (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... Cells are differentiated for 4 days in embryonic body (EB) differentiation medium (DMEM/F12 [Hyclone], 20% FBS, NEAA [1×, GIBCO], GlutaMAX [1×, GIBCO], 0.1% beta-Mercaptoethanol [GIBCO]). For mixed embryonic body (EB ...
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bioRxiv - Genomics 2019Quote: ... with 1% beta-mercaptoethanol for Smart-seq2 and 384-well plates containing 0.6 µl 1% NP40 (Thermo Fisher Scientific) for CEL-Seq2.
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bioRxiv - Cancer Biology 2020Quote: ... Tumor-bearing mice were administered 25 mg/ kg of MeTC7 or vehicle control (40% Hydroxypropyl-beta-cyclodextrin [Acros Organics] & solutol HS15 [Sigma] in sterile water ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Cell Biology 2021Quote: ... first dimensional lanes are cut from the gel and incubated in 1% SDS with 1% beta-mercaptoethanol for 1 hour before the gel slice was loaded into an NuPAGE 4-13% 1.0mmx2D gel (Invitrogen). Gels were run at 200V for 50 minutes at room temperature in MOPS ...
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bioRxiv - Microbiology 2020Quote: ... was suspended in 600 μL of Qiagen RLT lysis buffer supplemented with one percent beta-mercaptoethanol and 0.3 U/μL Superase-In (Invitrogen). The suspension was subjected to bead beating for 3 minutes using 1.0 mm Zirconia/Silica beads ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial pellets were suspended in 600 μL Qiagen RLT lysis buffer supplemented with one percent beta-mercaptoethanol and 0.3 U/μL Superase-In (Invitrogen). The suspension was subjected to bead beating for 3 minutes using 1.0 mm Zirconia/Silica beads ...
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bioRxiv - Neuroscience 2022Quote: ... mice received a unilateral injection of cholera toxin beta subunit coupled to Alexa Fluor 594 (CTb-594, Invitrogen C34777). Mice were anesthetized with isoflurane and treated with proparacaine ophthalmic drops (1%) ...
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bioRxiv - Neuroscience 2022Quote: ... Primer pairs specific to mouse RFC1/SLC19a1 (Mm00446220_m1) and Tubb4a (Mm00726185_s1, beta 4A class IVA as a housekeeping gene) were constructed and verified by Life Technologies to use with TaqMan qPCR chemistry (TaqMan™ Gene Expression Assay ...
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bioRxiv - Cancer Biology 2023Quote: The MCF7 cells were grown in their aforementioned media (see section 2.3) and treated with 10nM beta-estradiol in DMEM no phenol red (Gibco), 2% charcoal-stripped FBS (cFBS) ...
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bioRxiv - Biochemistry 2023Quote: ... 10x serial dilutions were made in 0.1% formic acid 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) in LCMS grade water ...
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bioRxiv - Neuroscience 2024Quote: ... Both fractions were then used to measure the amyloid beta 1-42 (Aβ1-42) using a specific ELISA kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) in 1X blocking buffer for 45 min ...
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bioRxiv - Systems Biology 2020Quote: ... fixed and blocked HeLa CCL-2 cells were incubated with 5 μg/mL rabbit anti-Ki67 (Thermo Fisher Scientific; RB1510P1ABX) for 45 min in 1X blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... the intestines were incubated 2 h at room temperature with secondary antibody Alexa Fluor 488 donkey anti-rabbit IgG (#A21206, Invitrogen) 1:600 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with secondary antibodies in vehicle for 2 h at room temperature (goat-anti-rabbit Alexa 546, 1:500, ThermoFisher A-11035 ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: ... Samples were then incubated for 2 h with anti-rabbit Alexa Fluor 594 secondary antibody (1:500; Thermo Fisher Scientific). Slices were washed and mounted on microscope slides ...