Labshake search
Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS and incubated (2 h) with secondary antibodies anti-rabbit or anti-mouse Alexa488 (1:1000, Invitrogen), and/or anti-rabbit or anti-mouse CY3 (1:1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue was then washed with PB and incubated overnight in PBT-X (2% NHS, 0.2% TritonX-100 in PB) with secondary antibody (1:1000 AlexaFluor 488-conjugate anti-rabbit, ThermoFisher Scientific). Tissue was washed with PB and mounted onto gelatinised slides ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated for 1 hr with goat anti-rabbit Alexa Fluor 488F(ab’)2 Fragment (1:1000, A11070; Invitrogen) and Alexa Fluor 568 Phallodin (A12380 ...
-
bioRxiv - Neuroscience 2020Quote: ... the free-floating sections were incubated for 2 h in staining solution together with anti-rat Alexa 594 and anti-rabbit Alexa 647 (1:400, ThermoFisher). Slices were washed 3 times with PBS and mounted using Dako Fluorescent mounting medium (Agilent) ...
-
bioRxiv - Neuroscience 2020Quote: ... and AlexaFluor 555-conjugated F(ab’)2 fragment goat anti-rabbit (1:500; A21428, Invitrogen, Thermo Fisher Scientific, Waltham, MA). After five 5-minute washes with PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... and AlexaFluor 555-conjugated F(ab’)2 fragment goat anti-rabbit (1:500; A21428, Invitrogen, Thermo Fisher Scientific, Waltham, MA). After five 5-minute washes with PBS ...
-
bioRxiv - Microbiology 2021Quote: ... in 10% FBS for 1-2 hours followed by incubation with secondary goat anti-rabbit AlexaFluor 594 antibody (1:1000, Invitrogen) in 10% FBS for 30 min ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... slices were incubated for 2 hours with a secondary anti-rabbit antibody conjugated to Alexa 488 (1:500, Fisher Scientific) and then washed in PBS at RT ...
-
bioRxiv - Microbiology 2020Quote: ... and incubation with the Alexa Fluor 647-conjugated secondary anti-rabbit antibody (Molecular probes Cat# A21245, RRID:AB_141775, 2 μg/μl), Acti-stain 488 phalloidin (Cytoskeleton #PHDG1 ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 hours at RT with secondary antibodies: goat anti-rabbit Alexa Fluor633 (1:500, Invitrogen, A21070), goat anti-mouse Alexa Fluor488 (Jackson Immunoresearch 115-545-003 ...
-
bioRxiv - Microbiology 2021Quote: ... and incubated for three hours at 37°C in 2% BSA in PBS complemented with secondary antibody (goat anti-rabbit-Oregon Green, goat anti-mouse-A594, Invitrogen). Gels were washed three times in PBST before a second overnight expansion in ddH2O ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2 h at room temperature with goat anti-rabbit (H+L) or goat anti-mouse HRP-linked secondary antibodies (Invitrogen) respectively ...
-
bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated in primary antibody (rabbit-anti-p65/RELA; 2 ug/mL; ThermoFisher, Waltham, MA, #10745-1-AP) diluted in blocking solution for 1h ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by 2-hour incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]). Following three additional 10-min washes in PBST ...
-
bioRxiv - Immunology 2022Quote: ... transfected HEK293T cells were stained with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) and an Alexa Fluor 488-conjugated goat anti-rabbit IgG (A11034, Invitrogen). Cells were then acquired on a BD Accuri C6 plus and analyzed by FlowJo software version 10.6.2.
-
bioRxiv - Immunology 2022Quote: ... Cells were then blocked with 20% FBS in PBS and incubated with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) followed by an Alexa Fluor 594-conjugated goat anti-rabbit IgG (A11037, Invitrogen). DNA was stained with Hoechst ...
-
bioRxiv - Neuroscience 2019Quote: ... larval brains were incubated for 2 hours at room temperature with secondary antibody Alexa Fluor 488-conjugated goat anti-rabbit (A-11034, ThermoFisher) and FITC-conjugated goat anti-HRP (123-095-021 ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by a 2 hour incubation period at room temperature with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen] ...
-
bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 hours with a secondary goat anti-rabbit IgG antibody conjugated with Alexa Fluor 647 (Invitrogen). The sections were then coverslipped and mounted on microscope slides ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... secondary antibodies were applied for 2 h in the dark (1:250 anti rabbit IgG coupled to AlexaFluor-555 [Invitrogen, A31572] and 1:250 anti mouse IgG coupled to AlexaFluor-488 [Invitrogen ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissue was washed and incubated for 2 hr at room temperature in goat anti-rabbit IgG conjugated to Alexa 594 (1:500, Invitrogen). Sections were again washed ...
-
bioRxiv - Microbiology 2022Quote: ... antibody and the secondary Alexa Fluor 488 (green) labeled goat anti-rabbit IgG (H + L; F (ab’)2 fragment) (Invitrogen) targeting the anti-E ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed for 60 min with TBST at room temperature and then incubated for 2 h at room temperature with goat anti-rabbit (H+L) HRP-linked secondary antibody (Invitrogen) respectively ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M ...
-
bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 times in TBS for 10 min each and incubated for 1 h at room temperature with a combination of 2 fluorescent dye-labelled secondary antibodies: a Cy3-conjugated goat anti-rabbit antibody (1:200; Invitrogen, Waltham ...
-
bioRxiv - Neuroscience 2023Quote: ... with TBST and incubated for 2 hours with horseradish peroxidase coupled secondary goat anti-rabbit or anti-mouse IgG (1:5000, Invitrogen) in TBST ...
-
bioRxiv - Cancer Biology 2023Quote: ... followed by incubation for 2 hours in secondary antibody (Alexa Fluor 633 goat anti-rabbit IgG (H+L) (Invitrogen A21071) 1:250 in block solution) ...
-
bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were washed once in PBS and incubated in the following secondary antibody and lipid droplet dye for 2 hr at RT: donkey anti-rabbit 555 (1:500; Invitrogen) and LipidSpot 488 (1:1000 ...
-
bioRxiv - Developmental Biology 2023Quote: ... followed with 2 hour incubation with anti-rabbit Alexa-fluor 488 conjugated secondary antibody (Thermo Fisher Scientific, Cat# A-11008) diluted 1:500.
-
bioRxiv - Immunology 2024Quote: ... After 2 hours incubation with HRP-conjugated secondary antibody (1:10,000; anti-mouse or anti-rabbit, Invitrogen #31430 and #31460) in TBST ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were incubated with one of the following secondary antibodies at RT for 2 hours: anti-rabbit Alexa Flour-488 conjugated antibody (Cat# A-11008, Invitrogen), anti-rabbit Alexa Flour-555 conjugated antibody (Cat# A-21428) ...
-
bioRxiv - Neuroscience 2024Quote: ... neurons were washed twice with 10% Formamide and 0.5% BSA in 2× SSC and incubated twice with goat anti-rabbit secondary antibody labeled with Alexa 647 (Invitrogen, A21245), 10% Formamide ...
-
bioRxiv - Molecular Biology 2023Quote: ... Purifications were carried out from 150 μl of the whole-cell extract (2 mg protein) per experiment using 37 µl of Dynabeads M-280 sheep anti-rabbit IgG (Invitrogen) and 2 µl of the anti-LytA antibody (Biomedal) ...
-
bioRxiv - Cell Biology 2020Quote: ... and recombinant SHH was purified with Detoxi-Gel™ Endotoxin Removing Gel (ThermoFisher) and dialyzed ...
-
bioRxiv - Plant Biology 2020Quote: All recombinant plasmids were generated by the GATEWAY cloning system (Invitrogen, http://www.invitrogen.com/). The primers used for gene amplification are listed in S3 Table ...
-
bioRxiv - Developmental Biology 2021Quote: ... Recombinant lentivirus was produced by transfecting HEK293T cells with Lipofectamine 2000 (Invitrogen, 11668019) or TransIT-2020 transfection reagent (Mirus ...
-
bioRxiv - Biochemistry 2022Quote: Recombinant proteins were labeled with Alexa Fluor(tm) 647 NHS ester (Thermo Fisher) as described previously18 ...
-
bioRxiv - Biochemistry 2022Quote: ... the recombinant protein mixtures were combined with 12.5 μM diFMUP (Life Technologies, E12020), added to 384-well plates ...
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant adenovirus plasmids were homologously recombined with pAd/CMV/V5-DEST vectors (Invitrogen), as per manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
-
bioRxiv - Developmental Biology 2020Quote: ... Recombinant lentiviruses were produced by transient transfection in HEK293FT cells (Invitrogen, CA, USA), as described earlier 18 ...
-
bioRxiv - Genomics 2019Quote: ... 4) We used RNase Inhibitor in place of RNaseOUT Recombinant Ribonuclease Inhibitor (Invitrogen) in the first-strand RT reaction ...
-
bioRxiv - Immunology 2019Quote: Chimeric VLPs or monomeric recombinant proteins were coated onto Maxisorp microtiter plates (Nunc) at 1μg/ml in PBS and incubated overnight at 4°C ...
-
LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... the recombinant proteins were dialyzed using Slide-A-Lyzer™ cassettes (Thermo Fisher) into cold coupling buffer containing 100mM NaHCO3 and 500mM NaCl ...