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Citations for Thermo Fisher :
1451 - 1500 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Sections were then incubated with secondary antibodies at room temperature for 2 h at 1:500 dilution: anti-rabbit AF594 (Invitrogen, A21207); anti-rat AF488 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were washed three times with 5 mL of 0.1% Triton X-100/PBS and incubated with 2 μL of anti-rabbit IgG secondary-APC (Invitrogen, A-10931) in 100 μL of 0.1% Triton X-100/PBS for 40 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were rinsed 2 times with PBS and incubated for 2 hours in 1:5000 Alexa Fluor 750 goat anti-rabbit secondary antibody (Life Technologies). The sections were rinsed 2 times with PBS and mounted in ProLong diamond antifade mounting media (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were stained with rabbit α-desmin and respective secondary antibody (Supplementary Table 2) and Hoechst 33342 (Thermo Fisher Scientific), and imaged using an ImageXpress Pico Automated Cell Imaging System (Molecular Devices) ...
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bioRxiv - Physiology 2024Quote: ... then incubated at RT for 2 h with goat anti-Rabbit IgG (H+L) Alexa Fluor Plus 488 (ThermoFisher, cat#A32731) and goat anti-guinea pig IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GFP rabbit (A11122, Invitrogen, CA, USA), chicken anti-GFP (AB16901 ...
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bioRxiv - Immunology 2021Quote: ... mouse serum samples were added to rat anti-mouse IgE mAb-coated 96-well Nunc MaxiSorb plates (Thermo Scientific). An HRP-conjugated goat anti-mouse IgE antibody (SouthernBiotech ...
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bioRxiv - Bioengineering 2019Quote: STP and mAb binding to the carriers was quantified indirectly using a bicinchoninic acid assay (BCA) kit (Thermo Fisher) according to the manufacture’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAb anti-ZO-1 (clone ZO1-1A12) used in immunofluorescence 1:300 on zebrafish embryos was from Invitrogen. Rat mAb anti-HA High Affinity (clone 3F10) ...
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bioRxiv - Microbiology 2020Quote: ... The resin was subsequently washed with 3 resin volumes (RV) of the same buffer before mAb P3.2 (Thermo Fisher) was eluted using 3 RV of 0.2 M glycine pH 2.5 ...
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bioRxiv - Microbiology 2021Quote: ... COV2-2196 and COV2-2130 mAbs were produced after transient transfection using the Gibco ExpiCHO Expression System (ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... used at 1:5,000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, pH7.5) + 10% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Microbiology 2020Quote: ... all mAbs were made as IgG1 and expressed in Chinese hamster ovary cells (ExpiCHO; Thermo Fisher Scientific, Waltham, MA). The mAbs were purified using HiTrap Protein A HP column (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Immunology 2020Quote: ... PE-Cy7-conjugated anti-human CD196 (CCR6) and PE-Cy7-conjugated anti-human MAVS mAbs were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Immunology 2020Quote: ... and α-CD28 (clone 9.3) mAbs at a concentration of 1 μg/ml in RPMI medium 1640 (Life Technologies) supplemented with 10% FCS and 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Endogenous TGF-βRII was probed using by two monoclonal antibodies (mAbs: sc-17792, Santa Cruz, and PA5-36115, ThermoFisher). Neither detected endogenous protein in our immunofluorescence or western blot studies.
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bioRxiv - Immunology 2022Quote: ... The supernatant containing secreted mAb was harvested at day 4 and purified using protein A-agarose beads (Thermo Fisher) as detailed previously56.
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bioRxiv - Microbiology 2024Quote: ... directed against the cytoplasmic domain of E-cad and a monoclonal anti-Occludin mouse mAb (331500, Life Technologies SA) and revealed using an anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... mAbs binding to the S proteins were detected using 25 µl/well of PNPP (p-nitrophenyl phosphate; Thermo Fisher) and the reaction was measured at a wavelength of 405nm using the Varioskan Lux Reader (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... The mAbs were detected using Alexa Fluor-594 conjugated goat anti-mouse IgG (H+L) (Invitrogen Cat# A-11032) and the reference Ab with an Alexa Fluor-488 conjugated goat Ab against the appropriate host (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Certain mAbs were loaded with 50 μL/well to 96 well microplates (Thermo fisher maxisorp, catlog No: M9410-1CS) and incubated overnight After three washes with PBS-T (PBS with 0.05% Tween 20) ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... The preparation of eVLPs expressing either Wuhan reference Spike or Beta variant Spike were formulated in Aluminum phosphate (Alum, Adjuphos®, Invitrogen) to obtain vaccine candidate VBI-2902a and VBI-2905a ...
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bioRxiv - Immunology 2022Quote: ... and then subjected to measurement of IL-1β protein using the Mouse IL-1 beta-uncoated ELISA kit (Invitrogen, Waltham, MA), following manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: Aß1-40 and Aß1-42 concentrations from cleared supernatants of differentiated iPSCs were quantified by using the Amyloid beta 40/ 42 Human ELISA Kits (#KHB3441 and KHB3481; Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2019Quote: ... A predesigned qPCR assay (Integrated DNA technologies) was used to detect the housekeeping gene beta actin and all samples were run on the StepOnePlus system (Applied Biosystems). In macrophage studies ...
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bioRxiv - Immunology 2021Quote: RBDwt was conjugated to CuMVTT using the cross-linker succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, Waltham, MA, USA) at 7.5 molar excess to CuMVTT for 30 min at 25 °C ...
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Corticocortical Connections of the Rostral Forelimb Area in Rats: A Quantitative Tract-Tracing StudybioRxiv - Neuroscience 2020Quote: ... Cholera toxin beta subunit conjugated to AlexaFluor 647 (CTB647, 5 µg/µL in 0.9% sterile saline, C34778, Invitrogen, Grand Island, NY) was injected (with the same configuration and outside diameter as BDA10kDa ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... the Anti-beta actin antibody was reprobed after stripping the membranes using Restore™ stripping buffer (Thermo Fisher Scientific, Cat# 21059).
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Cell Biology 2024Quote: The fluorescent cholesterol probe was tracked by incubating the cells with a final concentration of 20 μg/ml of TopFluor® Cholesterol (810255, Avanti) and 200 μg/ml of methyl-beta- cyclodextrin (C4555-1G, ThermoFisher) in PBS for 2 min at room temperature ...
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bioRxiv - Immunology 2022Quote: IL-1β ELISA was performed according to manufactures instructions using the Invitrogen IL-1 beta Mouse Uncoated ELISA Kit (Invitrogen, #88701388) with the exception that half-volume ELISA plates were used.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: mouse anti-Beta-actin (1:1000, Thermo Fisher Scientific, AM4302) and rabbit anti-PROX1 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Dried lysed cellular lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Biochemistry 2023Quote: ... Dried cell lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant baculovirus was generated using the Bac-to-Bac baculovirus expression system (Fisher Scientific) and was produced in Sf9 cells ...
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bioRxiv - Immunology 2021Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant antibody was labelled with Alexa Fluor 594 following the manufacturer’s instructions (Life technologies). Standard molecular biology techniques were used to introduce a cysteine residue at position 239 in the CH2 domain of each heavy chain (44) ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant baculovirus was produced using the Bac-to-Bac Baculovirus expression system (Thermo Fisher). The mouse utrophin (NP_035812 ...
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bioRxiv - Bioengineering 2021Quote: Recombinant baculovirus were generated by using a Bac-to-Bac expression system (Invitrogen, USA) according to the manufacturer’s instructions ...