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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... samples were incubated for 2 h at room temperature with secondary antibody Alexa 488 goat anti-rabbit IgG or rhodamine-phalloidin (1:500, Invitrogen, Molecular Probes). Samples were washed three times in 0.5% PBS-T and mounted in Vectashield containing DAPI.
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bioRxiv - Bioengineering 2022Quote: ... retinas were incubated for 2 hours at RT with 546-conjugated anti-rabbit IgG antibody (1/300; Life Technologies, Saint Aubin, France). Cell nuclei were labelled with Draq5 (1/500 ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBST and subsequently incubated for 2 hrs at room temperature in AlexaFluor™ 488-conjugated goat anti-rabbit (1:500, Invitrogen A11008) diluted in PBST containing 4% NGS ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed in PBS-T and incubated for 2 h (room temperature) with donkey anti-rabbit IgG (Alexa Flor™ 488; Invitrogen, A21206), donkey anti-goat IgG (Alexa Flor™ 568 ...
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bioRxiv - Pathology 2023Quote: ... appropriate secondary antibodies were treated for 1-2 hours in the dark (AF555 anti-Rabbit, AF555 anti-Rat, AF647 anti-Rat (Invitrogen, Waltham, MA)) and washed in PBS containing 0.01% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated for 2 hours with a secondary goat anti-rabbit IgG antibody conjugated with Alexa Fluor 647 (Invitrogen, A-21244). Nuclei were labeled by 4-,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Immunology 2023Quote: ... For hamster samples a SARS-CoV-2 Nucleocapsid antibody (SinoBiological #40588) followed by a goat anti-rabbit IgG Alexa Fluor 488 antibody (ThermoFisher Scientific #A32731) was used (6) ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 hours at room temperature before overnight incubation with rabbit polyclonal anti-ELAPOR1 antibody (Thermo Fisher Scientific, PA5-72691, 1:150). Cells were then washed three times with PBS for 15 min and incubated with anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... the cells were treated with 2 μg/ml Alexa Fluor 594- conjugated goat anti-rabbit IgG (catalogue no. A11032; Thermo Fisher Scientific). Nucleus were stained blue with Hoechst 33342 (1:5,000 dilution in PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... the brain sections were washed for 5 min x3 in 0.1% Tween 20/PBS and incubated in fluorescence tagged secondary antibodies for 2 hours at RT: Alexa Fluor 594 anti-rabbit (1:500, ThermoFisher Scientific, A11037), Alexa Fluor 488 anti-mouse IgG1 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton-X 100 and 2 % BSA in PBS: Alexa 488-conjugated donkey anti-rabbit (1:500, Life technologies/ Invitrogen, Cat# A11055), Alexa 555-conjugated donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.2% Triton-X 100 and 2 % BSA in PBS: Alexa 488-conjugated donkey anti-rabbit (1:500, Life technologies/ Invitrogen, Cat# A11055), Alexa 555-conjugated donkey anti-mouse (1:500 ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA pol I and II visualization was accomplished by blocking (2) and incubating samples with rabbit anti-p-UBF1 (1:100 dilution; Invitrogen, PA5-105512) and mouse anti-RNA pol II (1:100 dilution ...
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bioRxiv - Immunology 2024Quote: ... the slices were incubated for 2 hours at room temperature with a tertiary antibody in PBS-Triton 0.01%: Anti-Alexa Fluor 488 (Rabbit, Thermofisher Invitrogen, A-11094) 1:400 and for 1 hour at room temperature with the quaternary antibody ...
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bioRxiv - Immunology 2024Quote: ... 1:400 and for 1 hour at room temperature with the quaternary antibody: F(ab)’2-goat anti-rabbit IgG (H+L) A488 (Invitrogen, A-11070) 1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer followed by PBS wash 15 min three times and then incubation for 2 h at room temperature with Alexa Fluor 594 conjugated donkey anti-rabbit antibody (1:1000, ThermoFisher A-21207). Stained samples were then washed for 15 min three times in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit (1:500 goat anti-rabbit, ThermoFisher A11012) conjugated with two-photon-excitable fluorophores (488nm and 594nm ...
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bioRxiv - Microbiology 2024Quote: ... or rabbit (Donkey anti-rabbit AF594, Invitrogen, Cat# A11012). Cells were washed in 1x PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Immunology 2020Quote: ... The peptide was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 47 ...
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bioRxiv - Microbiology 2021Quote: ... The mutations for Alpha and Beta (Fig. 4A) were added in silico to the codon-optimized Wuhan strain and ordered as synthetic genes (GeneArt, Thermo Fisher Scientific) and cloned into the same backbone (Planas et al. ...
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bioRxiv - Physiology 2021Quote: The concentrations of IL-1β in tissue extracts of ovaries and livers were determined using an IL-1 beta Pro-form Mouse Uncoated ELISA kit (88-8014-22; Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... In immunofluorescence and western blot study we used the following antibodies: mouse Beta Tubulin loading control (BT7R) (Thermo fisher, cat no MA516308), mouse monoclonal CDK5 antibody (invitrogen ...
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bioRxiv - Neuroscience 2021Quote: The cells were lysed directly in the wells by addition of 300 μl Buffer RLT supplemented with 15 mM beta-mercaptoethanol (Thermo Fisher Scientific) after a wash with Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Neuroscience 2021Quote: The cells were lysed directly in the wells by addition of 300 μl Buffer RLT supplemented with 15 mM beta-mercaptoethanol (Thermo Fisher Scientific) after a wash with Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Immunology 2019Quote: ... SHIV RNA was visualized with the 1-Plex ViewRNATM ISH Tissue Assay Kit using SIVmac239 or Beta actin (positive control) probe sets and ViewRNA Chromogenic Signal Amplification Kit (ThermoFisher, Waltham, MA). These two sequential slides were indivudually imaged with a Zeiss AxioObserver microscope and AxioCam MRm camera ...
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bioRxiv - Immunology 2021Quote: ... and was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 10 ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2022Quote: ... mPSCs were cultured using N2B27-based medium with addition of LIF and inhibitors for GSK3-beta and ERK (NB2iL) comprised of 48% Neurobasal Medium (Gibco, Gaithersburg, MD), 48% Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Cell Biology 2023Quote: Supernatants of cell culture medium collected during medium exchanges were analysed for albumin (10242, Diagnostic Systems) and 3-beta-hydroxybutyrate (Autokit 3-HB, Fujifilm Wako) on an Indiko Plus chemical analyzer (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μM isopropyl-β-D-thiogalactopyranoside (IPTG) and 100 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside (X-gal) (Thermo Fisher Scientific). After incubation overnight at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: Xenopus immunostaining was carried out as previously described102 with a primary antibody against beta-tubulin (1:100, DSHB E7) and a secondary goat anti-mouse fluorescent antibody (1:250, Life Technologies A32723).
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bioRxiv - Immunology 2020Quote: ... Rabbit anti-Fezf2 and rabbit anti-involucrin were revealed with Cy3-conjugated anti-rabbit IgG (Invitrogen) and biotinylated anti-TPA was revealed with Alexa Fluor 488-conjugated streptavidin (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-TRF1 antibody (Goat Pab sc-1977, SantaCruz), or anti-PAR (Mouse Mab 10H ALX-804220, Alexis) recovered with Protein-G dynabeads (Invitrogen), run on PAGE together with input sample (1:20 of amount of immunoprecipitated proteins ...
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bioRxiv - Immunology 2022Quote: ... we labeled the gp120-specific antibody (A32 mAb) with Allophycocyanin (APC) following the manufacturer instructions (Zenon Human IgG labeling kit, Invitrogen) and measured cell binding by flow cytometry ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... The purified mAbs were immediately passed over a custom made rhTNFα affinity column (NHS-activated agarose beads, Thermo Fisher Scientific) in gravity mode ...
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bioRxiv - Neuroscience 2020Quote: ... 1 ml of Dynal sheep-anti mouse IgG paramagnetic beads were non-covalently coupled with 60 µg of anti-His mAb (Invitrogen) in PBS plus 0.1% immunoglobulin-free BSA (Sigma ...
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bioRxiv - Pathology 2021Quote: Recombinant Fab fragments were produced in HEK cells by introducing a stop codon at the end of CH1 and purified by affinity chromatography on AKTA Xpress Mab System (Cytiva) with UNICORN 5.11 software version (Build 407) using CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific), buffer exchanged to PBS using a HiPrep 26/10 desalting columns (Cytiva) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: After SDS-PAGE and Western blotting the membranes were probed with anti-NP MAbs R001 and 1C7 at 1:1000 diluted in Superblock Buffer (ThermoFisher). Then antigen-antibody complexes were detected using appropriate anti species HRP-conjugates and West Femto substrate.
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bioRxiv - Microbiology 2022Quote: ... non permeabilized samples were incubated with an anti-SAG1 mAb followed by Alexa 488-conjugated anti-mouse IgG antibodies (Invitrogen). After permeabilization with 0.5% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... used at 1:5,000 in MAB buffer (100 mM Maleic acid, 150 mM NaCl, and pH 7.5) + 20% heat-inactivated lamb serum (Gibco 16070096) + 2% blocking reagent (Sigma 11096176001) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins bound to GP96 were immunoprecipitated with an antibody against Grp94 (Rat, mAb, Enzo) and Dynabeads Protein G (Thermo Scientific), then examined using mass spectrometry analysis ...
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bioRxiv - Microbiology 2020Quote: ... Cleavage of junctional proteins was detected by Western blot analysis using specific antibodies against E-cadherin (Mouse, mAb, Thermo Scientific), p120-catenin (Rabbit ...
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bioRxiv - Microbiology 2021Quote: ... with the following changes: goat anti-rat and streptavidin Molecular Probes Alexa-Fluor-488 and 555 conjugated secondary mAbs (Invitrogen) were used at 4µg/ml ...
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bioRxiv - Immunology 2020Quote: ... The half-maximal inhibitory concentrations (IC50) of the evaluated mAbs were tested by the Varioskan LUX Microplate Spectrophotometer (Thermo Fisher), and calculated by a four-parameter logistic regression using GraphPad Prism 8.0.
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bioRxiv - Microbiology 2021Quote: ... The sequencing of the selected mAbs was performed according with the protocol described in the commercial kit BigDye Terminator v3.1 (Life Technologies), and for that purpose the same primers described for PCR were used ...
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bioRxiv - Microbiology 2019Quote: ... paired heavy and light chain plasmids for each mAb were expressed in Expi293F cells (Cat# A14527, ThermoFisher Scientific, Waltham, MA) in a 96-well format ...