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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for 6 hydroxy 2 methylquinazolin 4 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... and with NanoDrop One (ThermoFisher) with target OD 260/280 and OD 260/230 ratios of 1.8 and 2.0-2.2 ...
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bioRxiv - Microbiology 2021Quote: ... One-step Ultra TMB (ThermoFisher) was then used for detection ...
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bioRxiv - Immunology 2021Quote: ... one labeled with AlexaFluor647 (Invitrogen), were used in this panel in order to increase specificity of the detection of SARS-CoV-2-specific B cells ...
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bioRxiv - Immunology 2021Quote: ... one labeled with AlexaFluor488 (Invitrogen), one labeled with AlexaFluor647 (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... and NanoDrop One (Thermo Fisher).
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bioRxiv - Genomics 2023Quote: ... spectrophotometric (Nanodrop One, Fisher Scientific) determination of purity ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli One Shot TOP10 (Invitrogen) were transformed with the resulting vector ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 2-4 seconds at a blotting force of 4 and plunge-frozen in liquid ethane using a MarkIV Vitrobot (Thermo Fisher Scientific). The chamber was maintained at 8 °C and 100% humidity during freezing ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded with the calcium indicator Fluo-4 by incubating in supplemented NB medium that contained 2 µM of Fluo-4 AM (Invitrogen, Carlsbad, CA), and Pluronic F-127 (0.04% ...
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Lot 134874), and 4-(2-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, purity ≥ 99.%, CAT #BP310-1, Lot 052975) were from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted from stages 2-4 hr embryos using Trizol reagent (Invitrogen). RNA quality of three biological replicates was tested by Agilent Bioanalyzer ...
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bioRxiv - Bioengineering 2021Quote: FBS free culture media with 15mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Gibco) in DMEM/F12 supplemented with 1% P/S was used for all experiments performed in the lung on a chip devices ...
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) 1M (ThermoFisher, ref. 15630106) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... 1-2 x 107 cells were incubated in 4 µM PBS-diluted CFSE (Invitrogen) for 10 min ...
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bioRxiv - Immunology 2022Quote: Overnight cultured-T cells were stained with 2 μM fluo-4 AM (Thermo Fisher) in Hank’s solution (SL6080 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Neurons were treated with 2 μM Fluo-4 AM (Life Technologies, Grand Island, NY) and 0.02% Pluronic F-127 (Life Technologies ...
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bioRxiv - Synthetic Biology 2020Quote: ... were transfected with 2 µg plasmid DNA and 4 µL Turbofect (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Cells (typically 2-4×106) were lysed in 350μl Trizol reagent (Ambion, Life Technologies) and RNA extracted using the Direct-zol RNA Miniprep kit (Zymo ...
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bioRxiv - Immunology 2020Quote: Cells (typically 2-4×106) were lysed in 350μl Trizol reagent (Ambion, Life Technologies) and RNA extracted using the Direct-zol RNA Miniprep kit (Zymo ...
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bioRxiv - Microbiology 2022Quote: ... and 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco, Gaithersburg, MD, USA). Cells were maintained at 37 °C in a humidified incubator with 5% CO2.
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/ml CD40 protein (ThermoFisher, A42565) in fresh PBS ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed in 4% formaldehyde containing 2 mg/mL DAPI nuclear stain (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 2-4 μg of plasmid was mixed with 500 μL Opti-Mem (Gibco 31985070), after which 2.5 μL Plus Reagent and 8 μL Lipofectamine LTX (Invitrogen 15338100 ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher) to remove excess unbound biotin ...
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bioRxiv - Neuroscience 2023Quote: FOs from 2 or 3 independent differentiations were fixed using 4% paraformaldehyde (Thermo Scientific) in PBS (Gibco ...
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bioRxiv - Immunology 2024Quote: ... for 2 h at 4°C and processed through Zeba spin desalting columns (ThermoFisher) to remove excess unbound biotin.
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bioRxiv - Developmental Biology 2024Quote: ... sperm were loaded with 2 mM of Fluo-4 AM (Invitrogen, Carlsbad, California, USA) diluted in 10% (w/v ...
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bioRxiv - Biophysics 2024Quote: ... HEPES ((4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)) was obtained from Fisher Scientific (Pittsburg, PA). Peptides were reconstituted at 25 mg ml-1 in nuclease-free ultra pure water as per the manufacturer’s instructions and stored as aliquots at -20°.HP1α was reconstituted (0.5 mg ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Microbiology 2021Quote: ... 2×104 iBMDMs (6-well plates) were transfected in suspension with 20 nM siRNA using Lipofectamine RNAiMAX (Invitrogen) in 200 μ Opti-MEM I (ThermoFisher).AllStars negative-control siRNA (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... treated coverslips and incubated at 37°C for 2-6 hours in Dulbecco’s modified Eagle’s medium (F-12/DMEM; Invitrogen) supplemented with 1% fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated MEFs were plated in 2 wells of a 6-well plate and cultured overnight in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: C57BL/6 or db/db mice were ear-punched using a 2 mm Animal Ear-Punch (Fisher Scientific) and 1×108 CFU of bacteria topically applied to the entire mouse using sterile cotton swabs at Day 0 and Day 4 post ear-punch ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected as follows: 2 μg eGFP-SHLD3 constructs and 6 μl Lipofectamine 2000 (Thermo Scientific) were incubated in Opti-MEM for 20 min at room temperature before being added to the cells whose media had been changed to McCoy’s with 10% FBS and no antibiotics ...
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
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bioRxiv - Immunology 2023Quote: ... RD114 cells were seeded at 2 × 105 cells/well in a 6-well plate with DMEM (Gibco, USA) supplemented with 10% FCS (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: Skin samples were collected from the cheek of mice at the indicated time points with a 4- or 6-mm biopsy punch into cold RPMI 1640 medium (RPMI; Gibco) and minced into smaller pieces with surgical scissors ...
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bioRxiv - Plant Biology 2019Quote: ... The respective antibodies or IgG were coupled for 4-6 hour to Protein G Dynabeads (50µl, 10004D, Thermo Fisher Scientific) and subsequently incubated overnight with equal amounts of sonicated chromatin ...
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bioRxiv - Neuroscience 2021Quote: ... were harvested from male and female adult WT C57BL/6 mice and immersed in a 4% paraformaldehyde solution (PFA) made in phosphate-buffered saline (PBS, Cat #AAJ19943K2, ThermoFisher) and stored at 4 °C overnight prior to transfer to 15% sucrose solution in PBS for an additional 24 h ...
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bioRxiv - Microbiology 2020Quote: ... Organoids in expansion media grew in culture for 4-6 months and needed to be routinely passaged with TRYPLE (Gibco). To induce differentiation to a hepatocyte-like fate ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells in 6-well plates were transiently transfected with 4 µg of siRNA using Lipofectamine 2000 (Invitrogen). At 48h post-transfection ...