Labshake search
Citations for Thermo Fisher :
1151 - 1200 of 10000+ citations for 6 hydroxy 2 methylquinazolin 4 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 0.5:1:0.1 (4 μg/6-well dishes) using Lipofectamine LTX PLUS (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Grids were blotted for 4-6 seconds with blotforce of 15 on a Vitrobot Mark IV Vitrobot (ThermoFisher) and directly plunged into liquid-nitrogen-cooled liquid ethane.
-
bioRxiv - Cell Biology 2023Quote: Cells cultured in 6 well plates were washed with PBS and fixed with 4% Paraformaldehyde (11400580, Fisher Scientific) in PBS for 5min at room temperature (RT) ...
-
bioRxiv - Cell Biology 2024Quote: ... we used 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH; Thermofisher Scientific Cat. No. T204). Yeast cells were stained with 0.5µM TMA-DPH ...
-
bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
-
bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected with the indicated plasmids for 3h in serum depleted medium (Opti-MEMTM, ThermoFisher) using lipofectamine 2000 (Life Technologies) ...
-
bioRxiv - Microbiology 2022Quote: ... COS-7 cells grown on 13 mm cover slips were transfected with either the empty pcDNA3.1(+) vector or one expressing the SARS-CoV-2 E protein using Turbofect transfection reagent (ThermoFisher). At 24 h pt ...
-
bioRxiv - Developmental Biology 2022Quote: ... embryos at the desired developmental stage were fixed for one hour using MAD fixative (2 parts methanol [Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
-
bioRxiv - Biochemistry 2023Quote: ... Restriction-digested shuttle vectors -1-6 (33) and -2-1 (14) for wc-1 and wc-2 respectively were recombined with PCR (Thermo Fisher Scientific, Catalog # F549S) products amplified with primers bearing point mutations of wc-1 or wc-2 ...
-
bioRxiv - Genetics 2020Quote: ... RNA was quantified using a NanoDrop™ One (#ND-ONE-W, ThermoFisher Scientific) and 2 μg per sample were used for cDNA synthesis by performing a retro-transcription reaction with the SuperScript™ VILO™ cDNA Synthesis Kit (#11754050 ...
-
bioRxiv - Microbiology 2021Quote: ... as measured by NanoDrop One/One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific) was loaded onto a 50-cm μPAC capLC column (PharmaFluidics ...
-
bioRxiv - Microbiology 2022Quote: NanoDrop One Microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific, catalog #: ND-ONE-W).
-
bioRxiv - Cancer Biology 2021Quote: ... RNA purity was assessed using a NanoDrop One (ThermoFisher Scientific, ND-ONE-W). The RNA samples (1μg ...
-
bioRxiv - Microbiology 2022Quote: ... One infected and one uninfected flask were treated with DSS Crosslinker (Thermo Scientific) following manufacturer instructions for intracellular crosslinking ...
-
bioRxiv - Biophysics 2022Quote: ... RNA was then quantified using a Nano Drop One (Thermofisher #ND-ONE-W) and the concentration adjusted to 60 ng/uL ...
-
bioRxiv - Physiology 2023Quote: ... One hundred microliters of One-step Ultra TMB ELISA substrate (Thermo Scientific #34028) was added to each well ...
-
bioRxiv - Immunology 2023Quote: ... the IgG concentration was determined using NanoDrop One (Thermo Fisher, ND-ONE-W). All monoclonal antibodies were assessed for integrity by SDS-PAGE analysis.
-
bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined using a NanoDrop One (Thermo Fisher, ND-ONE-W) using absorbance.
-
bioRxiv - Physiology 2019Quote: Total RNA samples were extracted from one thousand 4-7 day-old Culex female antennae with TRIzol reagent (Invitrogen, Carlsbad, CA). Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad ...
-
bioRxiv - Neuroscience 2020Quote: ... through one channel of each tetrode and subsequent transcardial perfusion with saline followed by 10% buffered formalin (SF100-4, ThermoFisher Scientifc). Brains were extracted and stored in fixative for one day ...
-
bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-CD63 (TS63) (1:500, #10628D, Life Technologies), anti-CD171 (L1CAM ...
-
bioRxiv - Neuroscience 2021Quote: ... The designated samples were then subjected to one-dimensional gel electrophoresis (1DE) employing precast NuPAGE 4-12% Bis-Tris 10-well mini protein gels (Invitrogen, Germany) with 2-[N-morpholino]ethanesulfonic acid (MOPS ...
-
bioRxiv - Developmental Biology 2022Quote: ... and incubated overnight at 4°C with 1 mg/ml BSA and 4% sheep serum in PBST and one or more of the following: Alexa-fluor 488 Phalloidin 1:300 (Molecular Probes), anti-beta tubulin antibody 1:100 (E7 ...
-
bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C on rotation and for one additional hour with mixed Dynabead protein G and A (20:10 µl, Life Technologies). Beads were washed with TSE- 150 (0.1% SDS,1% Triton ...
-
bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
-
bioRxiv - Neuroscience 2024Quote: ... were added and incubated for 4 hours at 4°c followed by one wash with lysis buffer (Pierce) with added Halt protease and phosphastase inhibitors (Thermo Scientific) and two washes with PBS and resuspended in PBS for Mass spectrometry.
-
bioRxiv - Biochemistry 2023Quote: ... A drop of 3.5-4 µl sample was applied on one side in a Vitrobot (Vitrobot Mk IV, Thermo Fisher Scientific) at 4 °C and 100 % humidity ...
-
bioRxiv - Immunology 2020Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was diluted 1: 25 and 6 µl was used for one qPCR reaction with PowerUp™ SYBR™ Green Master Mix (Thermo Fisher Scientific, A25776). Relative gene expression was calculated using the ΔΔCT method relative to ACTB control.
-
bioRxiv - Microbiology 2021Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
-
bioRxiv - Genomics 2023Quote: ... the ORF4-6 genomic region was amplified directly from the clinical samples using the SuperScript™ IV One-Step RT-PCR System (Thermo Fisher Scientific, Waltham, MA). Primers were designed using Primer3 (26 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 mM of 4-(2-hydroxyethyl)-1-piperazine-1-ethanesulfonic acid (HEPES) (Gibco) and 1 ng/mL of human bFGF (Sigma-Aldrich) ...
-
bioRxiv - Biochemistry 2020Quote: Cultured DRG neurons were loaded with 4 μM Fura-2 AM (Life Technologies) in culture medium at 37°C for at least 60 minutes before use ...
-
bioRxiv - Cell Biology 2021Quote: LX-2 and TWNT-4 human HSCs were lysed in TRIzol (Life Technologies) and total RNA was purified following the supplied protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... 4 mL of 2 mM disuccinimidyl glutarate (DSG,Life Technologies, #20593, Carlsbad, CA) prepared in 1X PBS was added per 1 × 107 cells to the conical tube and the solution was mixed thoroughly by pipetting to remove clumps ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 mmol/l 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Gibco, Germany #15630056), 2.5 ng/ml human fibroblast growth factor-basic (hFGF2 ...
-
bioRxiv - Biophysics 2020Quote: ... 2 mM MgCl2) and 4 μM of the phosphobinding protein (PBP) (Life Technologies) to detect the inorganic phosphate (Pi ...
-
bioRxiv - Immunology 2020Quote: ... P2X7a 451L or P2X7a 451P were loaded with 2 μM Fluo-4 (Invitrogen) for 20 min at 4°C and 10 min at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1:4 pD614G-SARS-CoV-2-spike:pPyEGFP using Lipofectamine 2000 (Life Technologies), and media was replaced on day 1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... XEN cells were passaged every 2–4 days using TrypLE Express (Thermo Fisher) up to passage 20.
-
bioRxiv - Cell Biology 2022Quote: ... 9 μl of carboxyl latex bead (4% w/v, 2 μm; Invitrogen, C37278), and 2 μl of Concanavalin A (1 mg/ml ...
-
bioRxiv - Physiology 2023Quote: Freshly isolated cardiomyocytes were loaded with 2 μM Fluo-4-AM (Thermo Fisher) for 20 min at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 5% human serum (Valley Biomedical) ...
-
bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 10% human serum (Valley Biomedical ...
-
bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 5% human serum (Valley Biomedical) ...
-
bioRxiv - Cell Biology 2022Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (Invitrogen, Life Technologies, Grand Island, NY) supplemented with 10% human serum (Valley Biomedical ...