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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for 6 hydroxy 2 methylquinazolin 4 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) (Invitrogen, cat.#15630080), CellMask membrane dye (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... A pCR 4-TOPO or pCR- XL-2-TOPO plasmid (ThermoFisher, Waltham, Massachusetts) with an insert spanning ITS1-5.8S rRNA-ITS2-D1/D2 region of 28S rRNA from a pure culture strain was used as a positive control ...
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bioRxiv - Microbiology 2023Quote: ... and an additional 2 mM L-glutamine (Gibco; 4 mM total L-glutamine). Cells and parasites were incubated at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... Cyclopentylidene-[4-(4ʹ-chlorophenyl)thiazol-2-yl]hydrazone (CPTH2) was purchased from ThermoFisher Scientific and used at a 50µM concentration ...
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bioRxiv - Immunology 2022Quote: HeLa-CIITA cells were incubated in 6-well plates using 2-4.105 cells/well using OPTIMEM (Gibco) complemented with 10% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... before pooling desired fractions and concentrating with Pierce PES 2-6 mL concentrator columns (Thermo Fisher Scientific). Concentrated protein was aliquoted and flash frozen in liquid N2 before storage at -80°C.
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bioRxiv - Systems Biology 2022Quote: ... the minimal medium (DMEM with 2 mM L-glutamine) was supplemented with either IL6 (interleukin-6) (ThermoFisher), TNFα (tumor necrosis factor-alpha ...
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bioRxiv - Biochemistry 2022Quote: ... The quality of obtained microsomes was tested with 9-amino-6-chloro-2-methoxyacridine (ACMA; Invitrogen A1324) fluorescence quenching assays.
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bioRxiv - Molecular Biology 2023Quote: U2OS 2-6-3 cells (Shanbhag et al., 2010) were cultured in McCoy’s 5A (Modified) Medium (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... VeroE6 and MDCK ⍺2-6 cells were cultured in minimum essential medium (MEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Cell Biology 2020Quote: ... Cultured colonies from organoids were fixed with 4% PFA for 2-4 hrs at room temperature followed by immobilization with Histogel (Thermo Scientific) for paraffin embedding ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 mm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive ...
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bioRxiv - Biochemistry 2023Quote: ... NaHCO3 and 150 μL acetone containing 4 mg/mL 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide (L-FDAA, Thermo Scientific) was added ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Systems Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 μm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive high-performance benchtop quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μl of 6 μM samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies) or native gels (NativePAGE 4-16% Bis-Tris Gel ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Microbiology 2020Quote: 4 μl of purified spores (6×107 spores/ml) were mixed with 40 μl of NucBlue (Invitrogen, catalog #R37605) and incubated at 25°C for 40 min ...
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bioRxiv - Genomics 2020Quote: ... standards (3 x 10μl) and samples (6 x ∼18μl) were loaded on a 4-12% Bis-Tris Nu-PAGE (Life Technologies) gel for electrophoresis ...
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bioRxiv - Biophysics 2022Quote: ... thoracic and lumbar DRGs were harvested from 4 to 6 week old MrprD-GFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... Organ of Corti explants were isolated from C57BL/6 mice at postnatal day 4 in cold Leibovitz’s L-15 medium (cat. # 21083027, Gibco) and held in place by two glass fibers glued to the bottom of a Petri dish ...
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bioRxiv - Developmental Biology 2023Quote: ... Images were collected manually or automatically with 4-6 µs dwell time using Maps 3.11 software (Thermo Fisher Scientific) (Burel et al. ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Pathology 2022Quote: ... sections were incubated for 3h at room temperature with secondary antibodies (1:1,000, Life Technologies, Grand Island, NY). Fluorescence was observed with a Zeiss LSM710 confocal microscope.
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bioRxiv - Developmental Biology 2021Quote: ... for 1-3h at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific). Cells were washed in 1x PBS and fixed in 10% neutral buffered formalin (HT501128 ...
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bioRxiv - Cell Biology 2021Quote: ... 6 EV samples (2 per cell type, 1 ZIKV and 1 control each) were fixed in 2% paraformaldehyde (PFA; Fisher Scientific, Cat #50-980-487) and processed as cited 59 ...
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bioRxiv - Microbiology 2020Quote: ... 2 μL of the cloning mixture was transformed into 50 μL One Shot® TOP10 Competent Cells (Invitrogen, K4300-01) by gentle mix ...
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bioRxiv - Molecular Biology 2020Quote: ... Detection of SARS-CoV-2 N1 gene sequences and host RNase P mRNA from de-identified SARS-CoV-2 positive samples was carried out with the one-step TaqMan RNA-to-Ct 1-Step Kit (ThermoFisher), using 2 μL of undiluted PEARL extract ...
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bioRxiv - Biochemistry 2020Quote: ... one µg of small RNAs was mixed with 2 µg of HPDP-Biotin (ThermoFisher Scientific, 1 mg/ml in DMSO). The reaction was incubated for three hours at room temperature protected from light and mixed every 15 minutes ...
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bioRxiv - Genetics 2019Quote: ... BbsI-linearized piggyBac-U6 backbone was mixed with purified PCE product in a vector:insert 2:1 ratio and was directly used for transformation of One Shot MAX Efficiency DH5α-T1 Competent Cells (Invitrogen).
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bioRxiv - Microbiology 2021Quote: Total RNA from 2×105 (24-well format) MDMs was isolated at one day post-infection using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... 12.5μL of 2 × reaction buffer provided with the Superscript III one-step RT-PCR system with Platinum Taq Polymerase (Invitrogen, Darmstadt ...
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bioRxiv - Microbiology 2021Quote: ... and 2 µL of total RNA was subjected to One-Step RT-qPCR using Applied Biosystems QuantStudio 3 (ThermoFisher Scientific), with the following cycling conditions ...
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bioRxiv - Microbiology 2021Quote: ... The viral DNA was transfected into UT7/Epo cells (2×105) seeded one day prior using lipofectamine 3000 reagent (Invitrogen) according to the manufacturer’s instructions ...
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β-importins Tnpo-SR and Cadmus and the small GTPase Ran promote ovarian cyst formation in DrosophilabioRxiv - Developmental Biology 2021Quote: ... dissected ovaries were incubated for one hour at room temperature in Grace's media containing 10 μM 5-ethynyl-2′-deoxyuridine (EdU; Life Technologies). The following primary antibodies were diluted in blocking solution and used overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The enrichment phase was performed by using the One-Touch 2 device with the Ion Sequencing 400 Kit according to the manufacturer’s instruction (Life Technologies) (Moitinho et al ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Immunology 2021Quote: ... cells were alternatively pre-activated with IL-2 as above and one of: 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads Human T-Expander or Human Treg Expander) ...
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bioRxiv - Microbiology 2023Quote: ... The quantity and quality of eluted DNA were measured using NanoDrop One spectrophotometer and Qubit 2 (both by ThermoFisher Scientific) to ensure that samples meet the minimum requirements for sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: A one-step phosphopeptide IMAC enrichment procedure was performed using a 4 × 50 mm ProPac IMAC-10 analytical column (Thermo Fisher Scientific). For the JY cells an equivalent of 5 mg of protein was used for one IMAC purification while for the C ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was washed with PBS and then incubated for one hour at 4°C while rocking with 1 mg/mL EZ-Link NHS-SS-Biotin (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Pathology 2023Quote: ... cryotubes containing one million progenitors were thawed and seeded at 23,500 cells/cm² on Lab-Tek™ 4-well chamber slides (Thermofisher Cat# 154526PK) coated with collagen I (1:60 dilution) ...
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bioRxiv - Pathology 2023Quote: ... cryotubes containing one million progenitors were thawed and seeded at 23,500 cells/cm² on Lab-Tek™ 4-well chamber slides (Thermofisher Cat# 154526PK) coated with collagen I (1:60 dilution) ...
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bioRxiv - Immunology 2023Quote: ... 2,000 cells were resuspended in 1µL of ECM mixture and plated on one end of a well on a 4 well Labtek plate (Thermo Scientific, cat#154917). 0.8ng of CXCL9 (R&D systems ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...