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Citations for Thermo Fisher :
1101 - 1150 of 10000+ citations for 6 hydroxy 2 methylquinazolin 4 3H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Organoids were passaged every 4 to 6 days by dispersion with TrypLE Express (Invitrogen, Carlsbad, CA, USA) and re-embedded in Matrigel-GFR.
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bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... This was followed by foregut cell differentiation from day 4 - 6 using advanced DMEM/F12 (Gibco; 12634) with 2% B27 (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... This was followed by foregut cell differentiation from day 4 - 6 using advanced DMEM/F12 (Gibco; 12634) with 2% B27 (Gibco ...
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bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting plasmids were transfected into the One Shot OmniMAX 2-T1R Chemically Competent cells (Invitrogen, C8540-03) by heat shock ...
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bioRxiv - Synthetic Biology 2023Quote: Cloning reactions were transformed into One Shot™ ccdB Survival™ 2 T1R Competent Cells (Thermo Fisher Scientific) or ccdB-sensitive DH5α Escherichia coli via heat-shock ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The ligated product was transformed to One Shot™ ccdB Survival™ 2 T1R Competent Cells (Invitrogen, A10460), and selected on LB+Amp plates ...
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bioRxiv - Physiology 2020Quote: ... interleukin 6 (IL-6; Invitrogen, Carlsbad, CA), plasminogen activator inhibitor 1 (PAI-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) at 37 °C for 5 min in PBTX solution ...
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bioRxiv - Microbiology 2019Quote: ... and 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher), referred to as complete DMEM (cDMEM) ...
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bioRxiv - Microbiology 2019Quote: ... lentiviral containing supernatant and selected using 2-4 μg/ml puromycin (Gibco) for 3-4 days.
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A key interfacial residue identified with in-cell structure characterization of a class A GPCR dimerbioRxiv - Biochemistry 2019Quote: ... cells were incubated with 4 μM Rhod-2 (Life technologies, Eugene, USA) for 30 min at 37 °C in humidified air with 5% CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 2 h and eluted by TEV protease (Invitrogen) cleavage at 4 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was then flash frozen with 2-methylbutane (O3551-4, Fisher Scientific) and stored at −75°C until sectioning.
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bioRxiv - Neuroscience 2020Quote: ... Cells were first loaded with Fluo-4-AM (2 μM, ThermoFisher, F14201) in Neurobasal A+ media for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... On day 4 cells were passaged 1:2 with Stempro Accutase (Invitrogen) for 5 minutes at 37C and replated on hESC qualified Matrigel® ...
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bioRxiv - Microbiology 2020Quote: ... 10 ml 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), 24 mL 5% NaHCO3 (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... the cells were incubated with 4 μM Fura-2 AM (Invitrogen, F1221) and 0.4% Pluronic™ F-127 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Cells (2×10^4) were plated in phenol-free RPMI (Life Technologies) with 10% FBS in 384-well plates and treated with varying doses of BRM011 ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Gibco/Thermo Fisher Scientific (Grand Island ...
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bioRxiv - Bioengineering 2023Quote: ... 2 μL of a NeutrAvidin solution consisting of 4 μg NeutrAvidin (ThermoFisher) dissolved in 25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... and 2 mM MS(PEG)4 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) at 30 °C for 45 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 2-hydroxyazobenzen-4’-caryboxylic acid (HABA)-avidin complex was used (Thermo Fisher). Quantification was measured at 500 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... Alkaline phosphatase staining was performed using the one-step nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3’-indolyphosphate p-toluidine salt (BCIP) solution (Thermofisher).
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bioRxiv - Cell Biology 2021Quote: ... and one-fifth of the affinity purification (IP) were separated on Bolt 4-12% Bis-Tris Plus 15-well gels (Invitrogen), run in Bolt 1x MOPS SDS running buffer (Novex ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was eliminated from RNA extracts by digesting for one hour at 37°C with 4 U of TURBO DNAase (Ambion) followed by purification with MEGAclear Transcription Clean-Up kits (Ambion).
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bioRxiv - Microbiology 2021Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12 % Bis Tris precast gels (ThermoFisher) for 50 minutes at 200 V ...
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bioRxiv - Genomics 2022Quote: ... We estimated DNA quality using a NanoDrop One Microvolume UV-Vis Spectrophotometer and quantified DNA using a Qubit 4 Fluorometer (ThermoFisher) with a Quant-iT dsDNA HS Assay Kit ...
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bioRxiv - Microbiology 2022Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) for 50 minutes at 200V ...
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bioRxiv - Cell Biology 2019Quote: ... Mixed protein eluates were separated by one-dimensional gel electrophoresis on a 4–12% NuPage Novex Bis–Tris gel (Invitrogen), stained with the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... One to 20 μg of total protein were separated by NuPAGE™ 4-12% Bis-Tris Gel (Thermo Fisher Scientific) and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... 16 mL of HMA-10 buffer was added and the preparation was put on a shaking rocker for one hour at 4 °C with 3 units/mL Rnase-free Dnase (Ambion) added ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 X 105 PC3 or MCF7 cells were plated in either 6 well culture dishes (Nunc™ ...
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bioRxiv - Molecular Biology 2020Quote: ... 6’-diamidino-2-phenylindole (DAPI) for nuclear staining (Fluoromount-G™ Mounting Medium, with DAPI, Invitrogen), and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of 2-6 μg/mL S was plated onto 384-well Nunc Maxisorp (ThermoFisher) plates in PBS and sealed overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and Type 6 primary probes targeting MYCN were designed and synthesized by Affymetrix (Supplementary Table 2). Hybridization was performed according to the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: 2-3 viable human slices were incubated with Fluo4-AM (6 μM, Invitrogen cat. No. F1221) for 1h in 3 mM HEPES buffer (125 mmol/l NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of reporters were transfected into cells in 6 well plate using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed twice with PBS and stained with Laurdan dye (6-dodecanoyl-2- dimethylaminoaphthalene) (Thermofisher) at 10 µM for 45 min ...
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bioRxiv - Bioengineering 2023Quote: ... KM-met released from CS-NP was quantified at 233 nm using a NanoDrop One microvolume UV-Vis spectrophotometer for up to 6 hours at room temperature (Thermofisher Scientific, Waltham, MA, USA). At the endpoint ...
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bioRxiv - Microbiology 2023Quote: ... A NanoDrop One (ThermoFisher) was used to measure the RNA concentration and purity ...
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bioRxiv - Genomics 2023Quote: ... Nanodrop One (Fisher Scientific) for purity ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Microbiology 2021Quote: ... L454W/E455G and S262R) and mCherry (6:4:1 mixtures; 2.2 μg/well) using Lipofectamine® 2000 (Invitrogen), according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... All experiments were performed between passages 4 to 6 where cells were dissociated using TrypLE express (Life technologies), a gentle animal origin-free recombinant enzyme and seeded at a cell density of 5000 cells/cm2.
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bioRxiv - Cancer Biology 2020Quote: ... mitochondria were purified from 4-6 × 107 cells using mitochondria isolation kit for cultured cells (Thermo Fisher Scientific) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... day 4- and day 6-control and mutant ESC-derived neurospheres were collected and treated with Trizol (Invitrogen). RNAs were quantified by a Nanodrop instrument ...