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Citations for Thermo Fisher :
1201 - 1250 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... AnkB440 KO primary cortical neurons were transfected with Halo-tagged AnkB440 or GFP-tagged AnkB220 plasmids at DIV0 by lipofection (Lipofectamine 2000, Thermo Fisher Scientific). After brain dissociation ...
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bioRxiv - Immunology 2022Quote: ... of the different constructs were ordered and cloned into a pPPI4 avidin-tagged and/or hexahistidine-tagged vector by PstI-BamHI digestion and ligation with Gibson Assembly (Thermo Fisher Scientific). The recombinant human ACE-2 receptor was obtained in the same way after ordering the corresponding gBlock gene fragment (Integrated DNA Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Immunology 2021Quote: ... RBD-His and Spike-his containing supernatants were batch purified using the HisPur™ Ni-NTA Resin (ThermoFisher Scientific). Supernatants were incubated with 6 ml of resin for 1h at RT ...
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Immunology 2019Quote: ... The products of PCR-amplified T7-gRNA were used as templates for in vitro transcription (MEGAshortscript T7 kit, Thermo Fisher Scientific). The gRNAs were purified using the MEGAClear Transcription Clean-up kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Each primer contains a T7 sequence at its 5’ end for use with the MEGAshortscript T7 transcription kit (Thermo Fisher Scientific). The reaction was placed in boiling water and allowed to cool to room temperature to promote annealing ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 mRNA for mouse zygote injection was in-vitro transcribed from the XbaI-linearized Cas9 plasmid with a T7 promoter using mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies). The sgRNAs were in-vitro transcribed from PCR products with the MEGAshortscript Kit (Life Technologies) ...
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bioRxiv - Biochemistry 2020Quote: ... A T7-gRNA product was amplified by PCR and used for in vitro transcription using the MEGAshortscript T7 kit (Invitrogen AM1345). The in vitro transcribed gRNA was purified with the MEGAclear kit (Invitrogen AM1908) ...
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bioRxiv - Biophysics 2020Quote: Purified PCR products were used as the templates for in vitro transcription with the T7 MEGAScript T7 Transcription kit (ThermoFisher #AMB13345). Standard reaction conditions were used ...
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bioRxiv - Physiology 2022Quote: In vitro transcription from the gRNA template or a Cas9 plasmid (T7-Cas9-HA-2NLS) was performed to produce gRNA or Cas9 mRNA using mMESSAGE mMACHINE T7 ULTRA kit (Invitrogen, AM1344). The ssODN stock solution was prepared according to manufacturer’s recommendation (IDT Technologies) ...
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bioRxiv - Genomics 2022Quote: ... the T7-sgRNA PCR products were used as the template for in vitro transcription with the MEGAshortscript T7 kit (Life Technologies) and the products were purified using the MEGAclear kit (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... the non-structural gene 4 (NS4) was amplified with primers containing the T7 promoter sequence followed by reverse transcription with MEGAscript T7 Transcription Kit (Thermo Fisher) to generate AEFV NS4 RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Obtained dsDNA was incubated with T7 RNA polymerase overnight at 37°C using the MEGAscript T7 Transcription Kit (Thermo Fisher Scientific). crRNAs were purified using RNAXP clean beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... A T7 RNA polymerase promoter sequence was added to allow for in vitro transcription using the MEGAscript T7 transcription kit (Thermo Fisher). RNA was end labelled at the 3’ end with biotin using the Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: Cas9 mRNA for zygotic injections was generated from the XhoI-digested pCMV/T7-hCas9 plasmid (Toolgen) using the mMESSAGE mMACHINE® T7 ULTRA Transcription Kit (Ambion) and purified using RNeasy Mini Kit (Qiagen).
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bioRxiv - Developmental Biology 2021Quote: The T7-sgRNA PCR product was gel-purified and employed as the template for IVT using the MEGAshortscript T7 kit (Life Technologies). Both the Cas9 mRNA and Peg10-ORF2-sgRNA were DNase-treated to eliminate template DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Linearized template DNA (amplified from plasmid with T7/SP6 primers) was used to synthesize RNA probes with either T7 or SP6 RNA polymerase (Life Technologies), and DIG-labeling mix (Roche ...
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bioRxiv - Microbiology 2023Quote: ... In-vitro transcription of RNAs by T7 RNA polymerase was carried out using the MEGAscript™ T7 kit (Thermo Fisher Scientific) according to the manufacturer’s instructions and RNA quality was checked by electrophoresis on 6 or 10% PAA/7 M urea gels ...
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bioRxiv - Molecular Biology 2023Quote: ... The T7-sgRNA PCR product was gel-purified and employed as the template for IVT using a MEGAshortscript T7 kit (Life Technologies). Both the hCas9 mRNA and Rtl8a ...
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bioRxiv - Microbiology 2023Quote: BHK-T7 cells expressing the T7 polymerase (46) were maintained in Dulbecco′s modified Eagle′s medium (DMEM) (Invitrogen life technologies) supplemented with 10% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting plasmids were then linearized and used as a template for run-off T7 transcription using the MEGAscript T7 transcription kit (Invitrogen AMB13345). 0.05 pmol of mRNA was incubated in a 10 µL in vitro translation reaction containing 4 µL of Hela cytoplasmic extract ...
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bioRxiv - Bioengineering 2022Quote: ... The secondary antibodies tagged with Alexa Fluor 647 (Invitrogen) at 5 μg/mL were added to the slices and incubated for 1 h before three-times washing and imaging.
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bioRxiv - Molecular Biology 2021Quote: ... or Alexa647-tagged antisera (1:500; Invitrogen, Karlsruhe, Germany) in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... tagged with Alexa Fluor 594 or 488 dyes (Invitrogen), and the signal enzymatically augmented with sequential tyramide signal amplification (TSA amplification kits ...
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bioRxiv - Physiology 2020Quote: ... Amp-3 and HRP-tagged probe (Thermo Fisher Inc), one drop each for 30 ...
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bioRxiv - Immunology 2021Quote: ... and Rhodamine or Alexa Fluor 488-tagged Phalloidin (Invitrogen) were used for counterstaining cells ...
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bioRxiv - Plant Biology 2023Quote: ... antibodies and detected using fluorescently tagged secondary antibodies (Invitrogen, goat raised anti-mouse 488 and Anti-rabbit 555) ...
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bioRxiv - Molecular Biology 2023Quote: ... Whi5 tagged with GFP was obtained from Thermo Fisher Scientific GFP yeast clone collection ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were analyzed by Western blot using rabbit anti-TEV cleavage site primary antibody (ThermoFisher, PA1-119) and visualized with a compatible near-infrared secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: The pET28-MBP-TEV-based constructs were transformed into BL21 Star (DE3) (Thermo Fisher Scientific, Cat# C601003) E ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Immunology 2022Quote: All experiments were performed at 4°C using ProtoArray Human Protein Microarrays (Thermo Fisher Scientific). Microarrays were blocked for 1 h in blocking solution (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... HK-2 cells were maintained in keratinocyte serum-free (KSF) media with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco - Thermo Fisher Scientific, MA, USA). All culture conditions contained a 5% antibiotic / antimycotic mixed solution (Nacalai Tesque) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human IFNγ (285-IF) was purchased from R & D Systems (Minneapolis, MN) and mouse IFNγ (BMS326) from eBioscience (Thermo Fisher Scientific, Waltham, MA). The following antibodies were used for Western blot ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5% fetal bovine serum except for RWPE-1 and RWPE-2 cell lines which were grown in a keratinocyte serum-free medium supplemented with 5 ng/mL recombinant human epidermal growth factor (EGF) and 50 μg/mL bovine pituitary extract (Gibco™, Invitrogen, Carlsbad, CA, USA). The cell lines were tested for short tandem repeat (STR ...
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bioRxiv - Cancer Biology 2024Quote: ... PWR-1E and PIN cells were cultured in Keratinocyte Serum-Free Medium supplemented with bovine pituitary extract and recombinant human epidermal growth factor (Gibco, Life Technologies, Grand Island, NY). Myc-CaP ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
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bioRxiv - Immunology 2021Quote: Hela cells stably-expressing EGFP-tagged NLRP3 and mApple-tagged RAB5A were seeded into Nunc™ Lab-Tek™ 8-well Chambered Coverglass (155411, ThermoFisher Scientific) on the day before experiments ...
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bioRxiv - Biochemistry 2020Quote: CHO-K1 cells in 10-cm dishes were transfected with complementary DNA encoding FLAG-tagged mLPCAT2 or one of several FLAG-tagged mLPCAT2 mutants using Lipofectamine 2000 (Thermo Fisher Scientific, USA). At 48 h post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Stable transformants of ZP161 expressing rat Pex14 variants tagged with N-terminal hexahistidine (His-Pex14) were isolated by transfection of pcDNAZeo-D/His-RnPEX14 variants (see below) followed by selection with Zeocin (Invitrogen), as described (Okumoto et al. ...