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Citations for Thermo Fisher :
1251 - 1300 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Lactic Acid Containing Polymers Produced in Engineered Sinorhizobium meliloti and Pseudomonas putidabioRxiv - Microbiology 2019Quote: ... the gel was used for His-tag staining following the InVision(tm) His-tag In-Gel Stain protocol provided by Invitrogen.
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bioRxiv - Immunology 2022Quote: ... pAc5.1A/V5-His-CBF and pAc5.1A/V5-His-EF-1α were transfected into S2 cells using Cellfectin II Reagent (Invitrogen, USA), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was added to 15 mL of glutathione agarose resin (which binds up to 40 mg of purified recombinant GST protein per milliliter of resin) (Thermo Scientific) and incubated on ice (4°C ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 spike protein was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2021Quote: Monoclonal antibodies or recombinant SARS-CoV-2 RBD + SBD1 or NTD proteins were transfected in FreeStyle 293F suspension cells (Life Technologies) using PEIMAX transfection reagent (Polysciences) ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Microbiology 2020Quote: ... RNA was transcribed from PCR products encoding the desired bases downstream of a T7 promoter region using MEGAScript T7 Transcription Kit (Invitrogen, Carlsbad, CA) followed by purification using the MEGAClear Transcription Clean-Up Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse primers included a leading T7 promoter sequence to facilitate probe synthesis by in vitro transcription using an Ambion MegaScript T7 kit (Thermo Fisher Scientific) supplemented with digoxigenin-11-UTP (Roche ...
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bioRxiv - Genetics 2019Quote: ... The linearized Cas9 plasmids were transcribed into mRNA using the T7 m MESSAGE Kit (Ambition, USA) and gRNA was synthesized using transcript Aid T7 High Yield Transcription Kit (Thermo Scientific, USA). Zebrafish embryos at the one-cell stage were co-injected with 20pg target gRNA and 300pg Cas9 mRNA ...
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bioRxiv - Molecular Biology 2019Quote: ... the resulting PCR was used as a template for T7 RNA polymerase transcription in vitro (MEGAshortscript™ T7 Transcription Kit, Thermo Fisher).
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bioRxiv - Genetics 2019Quote: ... The PCR amplified T7-sgRNA product was used as template for in vitro transcription using the MEGAshortscript T7 kit (Thermo Fisher Scientific). The injection mix consisted of Cas9 mRNA (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... dsRNA was then generated by incubating each PCR product with T7 enzyme using the MEGAscript™ T7 Transcription Kit (Thermo Fisher Scientific), purifying the dsRNA product by phenol-chloroform extraction ...
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bioRxiv - Genetics 2021Quote: ... The DNA was then linearized and used as a template for T7 RNA in vitro transcription using the T7 MEGAshortscript™ Kit (ThermoFisher AM1354) or Thermo T7 RNA polymerase (TOYOBO ...
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bioRxiv - Microbiology 2022Quote: ssrSSa and sprB were in vitro transcribed from a PCR product template T7 promoter-containing (primers listed in Table S3) with MEGAscript T7 transcription kit (Thermo Fisher Scientific). RNAs were separated on 8 % polyacrylamide-7 M urea gel electrophoresis and eluted overnight in G50 elution buffer (20 mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... sgRNAs were generated by PCR amplifying the sgRNA sequences with a primer introducing a T7 promoter upstream of the sgRNA and performing in vitro transcription using MEGAshortscript™ T7 Transcription Kit (Thermo Fisher). After digestion ...
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bioRxiv - Molecular Biology 2020Quote: ... purified dsDNA from the previous step was incubated with T7 RNA polymerase overnight at 37°C using the MEGAscript T7 Transcription Kit (Thermo Fisher Scientific), and RNA was purified with MEGAclear Transcription Clean-up kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: The 5’-UTRs of transcripts were amplified from MIN6 cell cDNA with the addition of a T7 promoter and in vitro transcribed using the AmpliScribe™ T7-Flash™ RNA transcription kit (Ambion) and biotin-16-CTP (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR product serves as the template for in vitro T7 transcription using the MEGAshortscript™ T7 Transcription Kit (Invitrogen™, AM1354). In vitro transcription was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid minipreps were sequenced by first being PCR-amplified using the T7-Prom-F/T7-Term-R primers and cleaned up using the ExoSAP-IT PCR Product Cleanup kit (Thermo Fisher, 78200). Sanger sequencing was then carried out by SourceBioscience for verification.
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bioRxiv - Cell Biology 2024Quote: ... This linear DNA template containing the T7 promoter was used to transcribe sgRNAs with the MEGAshortscript™ T7 Transcription Kit (Invitrogen; AM1354). The synthesized gRNAs were precipitated with ethanol and dissolved in nuclease-free water.
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bioRxiv - Cell Biology 2020Quote: ... His-tags were removed with AcTEV protease (Invitrogen) overnight and proteins further purified with a HiTrap SP HP cation exchange column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: A pcDNA3.1/V5-His TOPO expression vector (Invitrogen) encoding the protein-coding sequence of human SM (NM_003129.4 ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-His antibody (Thermo Fisher, Waltham, MA) was diluted 1:2000 in 1x iBind solution ...
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bioRxiv - Biochemistry 2020Quote: ... into pT7-N-His-GST (Thermo Fisher Scientific) or pET28a (EMD Millipore) ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 10% HI-FBS (16140-071, Gibco), 100 U/mL Penicillin-Streptomycin (15140122 ...
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bioRxiv - Biochemistry 2021Quote: ... then cloned into pIB/V5-His vector (Invitrogen), fused with V5 epitope at the C-terminus ...
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bioRxiv - Biophysics 2020Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
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bioRxiv - Microbiology 2022Quote: ... and mouse anti His Alexa Fluor 555 (Invitrogen catalogue number MA1-135-A555 ...
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bioRxiv - Biochemistry 2021Quote: ... 10 % v/v Hi-Di formamide (Thermo Scientific), supplemented with 2 mM vanadyl ribonucleoside complex) ...
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bioRxiv - Cancer Biology 2020Quote: ... in 1X Hi-Fi PCR buffer (Invitrogen #52045) supplemented with 2 uL of 50 mM MgSO4 and dNTPs (Roche#11-581-295-001 ...
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bioRxiv - Genetics 2021Quote: ... and Hi - Di™ Formamide (Applied Biosystems, USA). The DNA (PCR products ...
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bioRxiv - Microbiology 2020Quote: ... MprA was detected using Anti-His antibody (Invitrogen) and anti-mouse HRP-labelled secondary antibody (Santa Cruz) ...
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bioRxiv - Immunology 2022Quote: ... 10% hi-FBS and 1% penicillin/streptomycin (ThermoFisher) in absence or presence of APRIL (Recombinant Human APRIL/TNFSF13 Protein ...
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bioRxiv - Microbiology 2022Quote: ... DMEM 2% Hi-FBS 0.5% Ultrapure agarose (Invitrogen) was used to overlay the wells ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-His (Invitrogen, #MA1-21315, 1:1000), rabbit anti-GST (Merck,#ZRB1223,1:1000) ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% (v/v) HI-FBS (Gibco), 10 U/mL penicillin and 100 U/mL streptomycin (Corning) ...
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bioRxiv - Microbiology 2023Quote: ... anti-6x-His mouse monoclonal (Invitrogen 37-2900), anti-CD99 mouse monoclonal (Invitrogen MA5-12954) ...
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bioRxiv - Biophysics 2023Quote: ... HRP-conjugated anti-His antibody (Invitrogen – Thermo Fisher), anti-H3 antibody (abcam ab1791 ...
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bioRxiv - Biophysics 2023Quote: ... HRP-conjugated anti-His antibody (Invitrogen – Thermo Fisher), anti-H3 antibody (abcam ab1791 ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (v/v) HI-FCS (Gibco), 50 μM mercaptoethanol (Sigma-Alrich) ...