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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for Recombinant Human CD96 Protein T7 His TEV tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 10 U/μL T7 polymerase (ThermoFisher Scientific), 1 U/μL RiboLock RNase Inhibitor (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2019Quote: ... using a mMESSAGE mMACHINE T7 kit (Ambion). Oocytes ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1x T7 MEGAscript Transcription Buffer (Ambion). After a total incubation for 3 hours at 37°C ...
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bioRxiv - Genomics 2021Quote: ... The MEGAscript T7 Kit (Thermo Fisher Scientific) was used to generate dsRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... T7 or T3 mMessage machine kit (Ambion), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and MEGA shortscript T7 kit (Life Technologies), respectively ...
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bioRxiv - Cell Biology 2020Quote: mRNAs were transcribed using T7 mMessageMachine (Thermofisher) using manufacturer’s recommendation as described (Lee et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... transcribed (MEGAscript T7/SP6 ThermoFisher Scientific, AM1333), and cleaned up (Zymo Research ...
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bioRxiv - Immunology 2022Quote: ... and mMESSAGE mMACHINE T7 Ultra Kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... by MEGAscript® T7 Kit (Ambion, USA). The labeling process involved utilizing a BamHI-digested plasmid as a template ...
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bioRxiv - Developmental Biology 2023Quote: ... by MEGAscript® T7 Kit (Ambion, USA) using BamHI digested plasmid as a template ...
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bioRxiv - Developmental Biology 2023Quote: ... with the mMACHINE T7 Ultra kit (Ambion). The Tol2 mRNA was generated using the same protocol ...
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bioRxiv - Genetics 2023Quote: ... A MEGAshortscript T7 Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... with the MEGAshortscript T7 kit (Ambion, AM1354M). 1 nl of a mixture of 700 ng of the Cas9 protein (Toolgen ...
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bioRxiv - Microbiology 2024Quote: ... The mMESSAGE mMACHINE T7 transcription kit (Invitrogen) was used to generate the replicon RNAs in the correct orientation from the linearized vector according to manufacturer’s instruction ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... MEGAscript T3 or T7 RNA polymerases (Thermofisher) were used to synthesize complementary RNAs from PCR templates ...
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bioRxiv - Neuroscience 2019Quote: ... All proteins were expressed in Human Embryonic Kidney (HEK) 293 Freestyle cells (Invitrogen) in suspension culture using serum-free media ...
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bioRxiv - Immunology 2023Quote: ... RBD proteins were expressed in-house in Expi293F human cells (Thermo Fisher Scientific) by transfection of the cells with purified DNA and polyethylenimine (PEI) ...
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bioRxiv - Biochemistry 2023Quote: Proteins from human cell lines were extracted in IP lysis buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... AHA-labeled proteins were detected with 1 μM alkyne-tagged Alexa Fluor-488 using Click-iT cell detection reagents (Thermo Fisher Scientific) for 45 min at room temperature as previously described (3).
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Molecular Biology 2021Quote: ... Co-purification of 6His-tagged PGC-1α protein domains was detected by western blot using anti-6His antibody (clone His.H8, ThermoFisher Scientific MA1-21315).
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bioRxiv - Cell Biology 2021Quote: ... and anti-TRIP4 antibodies were raised in rabbits against affinity purified N-terminally hexahistidine-tagged full-length proteins and purified with the respective antigens coupled to SulfoLink resin (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... SH-SY-5Y cells stably expressing GFP or a GFP-tagged construct of the protein of interest were cultured for at least six doublings in SILAC DMEM (89985; Thermo Fisher Scientific) supplemented with 10% dialyzed FBS (F0392 ...
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bioRxiv - Biophysics 2023Quote: RBL-2H3 cells stably expressing clathrin light chain A tagged with green fluorescent proteins were cultured in minimum essential medium (MEM) (Invitrogen 11095-09) with 20% fetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... ABE8e was expressed as an 8xHis tagged protein from a rhamnose-inducible promoter in BL21-Star DE3 cells with low RNase activity (Thermo Fisher Scientific). At an OD600 of ∼0.8 ...
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bioRxiv - Molecular Biology 2023Quote: ... GST pull-down of PARylated proteins using GST-tagged RNF146(100-182) or GST-tagged AF1521(K35E/Y145R) was done using the Pierce GST protein interaction pull-down kit (Thermo Fisher Scientific). Glutathione agarose resin (50μl ...
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Srs2 binding to PCNA and its sumoylation contribute to RPA antagonism during the DNA damage responsebioRxiv - Molecular Biology 2024Quote: ... The activated form of Mec1 was enriched by immunoprecipitating myc-tagged Ddc2 using Protein A beads and anti-Myc antibody (Thermo Fisher 9E10) for 2–4 h at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected with pDisplay encoding myc-tagged GluA1-GluA4-ATD or pCAGGS encoding FLAG-tagged Nrx or GFP with Lipofectamin 2000 (Invitrogen). On the following day ...
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bioRxiv - Microbiology 2020Quote: Human IFITM proteins and SARS-CoV-2 viral proteins were cloned into MaMTH N-term tagged Prey and C-term tagged Bait vectors respectively using Gateway cloning technology (ThermoFisher). Correctness of recombined insertions was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Cell Biology 2023Quote: U2OS were transfected with the MAC-tagged GTPBP8 expression vector or a control MAC-tagged GFP vector using Fugene 6 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Genomics 2020Quote: ... 1.5X T7 reaction buffer, 1.54 U/μl SUPERaseIN inhibitor and 2.3U/μl T7 enzyme; all from ThermoFisher Scientific, USA) was added and sealed with 40μl of Vapor-Lock oil (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of RNAs in vitro by T7 RNA polymerase was then carried out using the MEGAscript T7 kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Approximately 5 μg of BAC DNA was linearized with NotI and full-length RNA was obtained by in vitro transcription with T7 RNA polymerase followed by lithium chloride precipitation according to the manufacturer’s protocol (mMessage-mMachine T7 Kit; Ambion). 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031 ...
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bioRxiv - Microbiology 2022Quote: ... The resulting plasmids were linearized with XhoI prior to T7 RNA transcription using mMESSAGE mMACHINE T7 Transcription kit (Invitrogen). The pAVIC1- derived HAstV1 RNA was electroporated into BSR cells as previously described 16 ...
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bioRxiv - Neuroscience 2022Quote: ... Australia) and RNA transcribed by T7 RNA polymerase using the mMESSAGE mMACHINE T7 kit (Thermo Fisher Scientific, Sydney, Australia).
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bioRxiv - Immunology 2023Quote: ... The T7-sgRNA PCR product was purified and used as the template for IVT using MEGAshortscript T7 kit (Thermofisher). Both sgRNAs were purified using the MEGAclear kit (Thermofisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... and T7 promoter sequence that allowed for in vitro synthesis of sgRNA using MEGA Script T7 Kit (Invitrogen #AM1334). Synthesized sgRNA was mixed with Cas9 protein (NEB# M0646T ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA’s were in vitro transcribed from the T7 promoter using the mMESSAGE mMACHINE T7 Ultra kit from ThermoFisher Scientific according to the manufacturers protocol (#AM1345) ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Microbiology 2021Quote: ... recombinant SARS-CoV-2 Spike RBD protein (RBD, His Tag) was conjugated with EZ-LinkTM Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... IPS cells were thawed and cultured at all times cultured on recombinant human laminin LN521 (Biolamina, 2021-21) kept in E8 basal medium (Thermo Fisher Scientific) supplemented with primocin (0.1 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs were dissociated with TrypLE Express for 5 mins at 37°C and induced into EpiLCs by addition of human recombinant basic fibroblast growth factor (bFGF) (Invitrogen, 13256-029) and activin A (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: ... Phages displaying human Fabs were enriched after three rounds of biopanning on biotinylated recombinant human ACE2 immobilised to streptavidin Dynabeads (Dynal M-280, Invitrogen, cat # 112.06D). After the third round of panning ...
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bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were pulsed in starvation buffer supplemented with 0.1% w/v bovine serum albumin at 4 °C for 40 min with 50 ng/mL human recombinant EGF (Thermo-Fisher, Gibco™, PHG0311L) to allow ligand bind to the EGFR ...